Structured Review

TaKaRa xhoi
Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xhoi/product/TaKaRa
Average 94 stars, based on 518 article reviews
Price from $9.99 to $1999.99
xhoi - by Bioz Stars, 2020-05
94/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Reduced Folate Carrier: an Entry Receptor for a Novel Feline Leukemia Virus Variant
Article Snippet: .. PCR products were digested with EcoRI and BglII for feRFC and mRFC, and with XhoI and BglII for huRFC, and then each fragment was inserted into pMSCVneo retroviral vector (TaKaRa). .. The feRFC, huRFC, and feTHTR1 ( ) retroviral expression vectors were transfected into PLAT-E (ecotropic MuLV packaging) cells or PLAT-A (amphotropic MuLV packaging) cells using Lipofectamine 3000 (Invitrogen).

Clone Assay:

Article Title: Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus.
Article Snippet: .. This product was cut with XhoI and EcoRI, and the obtained gene was cloned into multiple cloning site (MCS) of pEGFP-N1 vector (Clontech), producing a p3b-EGFP plasmid. .. The pEGFP-3b, truncated 3b constructs were made in a similar fashion, and the oligonucleotide primers used were listed in Table 1 .

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: .. The cDNA of N-terminally FLAG-tagged mSiglec-14 was amplified using this construct as a template and primers “Xho1 PPT Fwd” and “p3×FLAG Seq Rev” (annealing to the downstream of ORF), digested with XhoI and NotI, and cloned to XhoI-NotI sites of pMSCVpuro (Clontech/Takara). .. The plasmid was designated FLAG-Siglec-14/pMSCVpuro.

Amplification:

Article Title: Voltage-Dependent Anion Channel Protein 2 (VDAC2) and Receptor of Activated Protein C Kinase 1 (RACK1) Act as Functional Receptors for Lymphocystis Disease Virus Infection
Article Snippet: .. Briefly, the amplified VDAC2 and pcDNA3.1 plasmids were doubly digested by HindIII and KpnI restriction enzymes (TaKaRa, Japan), the amplified RACK1 and pCIneo-GFP plasmids were doubly digested by XhoI and EcoRI restriction enzymes (TaKaRa), and the doubly digested genes and plasmids were then linked together using T4 DNA ligase (TaKaRa). .. The recombinant plasmid was transformed into competent E. coli DH5α cells (TaKaRa), followed by detection by colony PCR and sequencing.

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: .. The cDNA of N-terminally FLAG-tagged mSiglec-14 was amplified using this construct as a template and primers “Xho1 PPT Fwd” and “p3×FLAG Seq Rev” (annealing to the downstream of ORF), digested with XhoI and NotI, and cloned to XhoI-NotI sites of pMSCVpuro (Clontech/Takara). .. The plasmid was designated FLAG-Siglec-14/pMSCVpuro.

Plasmid Preparation:

Article Title: Reduced Folate Carrier: an Entry Receptor for a Novel Feline Leukemia Virus Variant
Article Snippet: .. PCR products were digested with EcoRI and BglII for feRFC and mRFC, and with XhoI and BglII for huRFC, and then each fragment was inserted into pMSCVneo retroviral vector (TaKaRa). .. The feRFC, huRFC, and feTHTR1 ( ) retroviral expression vectors were transfected into PLAT-E (ecotropic MuLV packaging) cells or PLAT-A (amphotropic MuLV packaging) cells using Lipofectamine 3000 (Invitrogen).

Article Title: Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus.
Article Snippet: .. This product was cut with XhoI and EcoRI, and the obtained gene was cloned into multiple cloning site (MCS) of pEGFP-N1 vector (Clontech), producing a p3b-EGFP plasmid. .. The pEGFP-3b, truncated 3b constructs were made in a similar fashion, and the oligonucleotide primers used were listed in Table 1 .

Construct:

Article Title: Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Article Snippet: .. The cDNA of N-terminally FLAG-tagged mSiglec-14 was amplified using this construct as a template and primers “Xho1 PPT Fwd” and “p3×FLAG Seq Rev” (annealing to the downstream of ORF), digested with XhoI and NotI, and cloned to XhoI-NotI sites of pMSCVpuro (Clontech/Takara). .. The plasmid was designated FLAG-Siglec-14/pMSCVpuro.

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  • 92
    TaKaRa xhoi restriction sites
    Lsa63 PCR Product, Double digestion of pET28 a(+)-Lsa63 and Western Blot of rLsa63 Protein A. lane 1, Kb DNA size marker; line 2, Lsa63-specific PCR products. B. Double digestion of <t>pET28a</t> (+)-Lsa63 using <t>NdeI/XhoI:</t> (left to right) line 1, 1 Kb ladder; line 2, recombinant plasmid; line 3, double digested product; line 4, PCR product of Lsa63. C. western blot of rLsa63 protein: (left to right) line 1, protein ladder; line 2, western blot of rLsa63.
    Xhoi Restriction Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi restriction sites/product/TaKaRa
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    xhoi restriction sites - by Bioz Stars, 2020-05
    92/100 stars
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    99
    TaKaRa 4 9 kb hindiii xhoi fragment
    Schemes of CHL1-GFP/GUS Constructs. (A) CHL1 gene structure. Closed boxes represent exons containing coding regions, open boxes represent 5′ and 3′ untranslated sequences, and numbers indicate nucleotides from the start of translation. (B) HaeII reporter construct with <t>∼4.9</t> kb of CHL1 promoter sequence and exon 1 (up to amino acid 34 of CHL1) fused in frame at the HaeII site to the coding sequence of GFP or GUS . NOS indicates the 3′ termination sequence of the nopaline synthase gene. (C) <t>XhoI</t> reporter construct with the 4.9-kb CHL1 promoter and intragenic sequences up to the middle of exon 4 (up to amino acid 269 of CHL1) fused in frame to the coding sequence of GFP or GUS .
    4 9 Kb Hindiii Xhoi Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 9 kb hindiii xhoi fragment/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4 9 kb hindiii xhoi fragment - by Bioz Stars, 2020-05
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    xhoi  (TaKaRa)
    99
    TaKaRa xhoi
    Generation of <t>IL23R-CHR</t> protein. A, Restriction sites are indicated in bold, the translated amino acid and Trx-6×His-DDDDKA tag are shown in the box; B, IL23R-CHR gene from human spleen cDNA library amplified by PCR; C, One pET32a/IL23R-CHR clone digested with NcoI and <t>XhoI</t> and analyzed by agrose gel electrophoresis (1%). MW = molecular weight markers, bp. D, Trx-IL23R-CHR induced by IPTG in E.Coli BL21 (DE3), SDS-PAGE (15%), protein bands were stained with coomassie brilliant blue R250 reagent. Lane 2, uninduced bacterial lysate; Lane 3, IPTG induced bacterial lysate. E, Trx-IL23R-CHR purified from BL21 (DE3) lysate and cleaved by enterokinase, Lane 5, purified Trx-IL23R-CHR; Lane 6, purified IL23R-CHR. F, Western blot using mouse mAbs against human IL23R (Lane 8). Lane 1, 4 and 7 in D, E, F: molecular weight markers, KD.
    Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi/product/TaKaRa
    Average 99 stars, based on 518 article reviews
    Price from $9.99 to $1999.99
    xhoi - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    91
    TaKaRa xhoi linearized pfl mus81 flag eme1
    Analysis of SMX Complex Formation throughout the Cell Cycle (A) Domain organization of human SLX1-SLX4, <t>MUS81-EME1,</t> and XPF-ERCC1, showing interactions between the SLX4 scaffold and the SLX1, MUS81, and XPF nuclease subunits. Interacting domains are denoted by double-headed arrows. Abbreviations for protein domains (top to bottom, left to right) are as follows: ERCC4, excision repair cross complementing 4; HhH, helix-hairpin-helix; WH, winged helix; UBZ, ubiquitin-binding zinc finger; MLR, MEI9 XPF interaction-like region; BTB, broad complex, tramtrack, and bric a brac; SIM, SUMO-interacting motif; SAP, C-terminal SAF-A/B, acinus, and PIAS; CCD, conserved C-terminal domain; SF2, superfamily 2; RING, really interesting new gene; GIY-YIG, conserved amino acids that form the catalytic motif. (B and C) Whole-cell extracts prepared from Flp-In T-REx 293 fibroblasts expressing <t>FLAG</t> SLX4 synchronized at G1/S (B) and G2/M (C) were centrifuged through 10%–45% sucrose gradients. Fractions were analyzed by western blotting for the indicated proteins. The positions of molecular weight markers are indicated. Boxed areas show the migration positions of SLX4-free MUS81-EME1 (fractions 7–9) and the SMX complex (fractions 11–14). Asterisks denote non-specific cross-reacting proteins. See also Figure S1 .
    Xhoi Linearized Pfl Mus81 Flag Eme1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi linearized pfl mus81 flag eme1/product/TaKaRa
    Average 91 stars, based on 1 article reviews
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    Image Search Results


    Lsa63 PCR Product, Double digestion of pET28 a(+)-Lsa63 and Western Blot of rLsa63 Protein A. lane 1, Kb DNA size marker; line 2, Lsa63-specific PCR products. B. Double digestion of pET28a (+)-Lsa63 using NdeI/XhoI: (left to right) line 1, 1 Kb ladder; line 2, recombinant plasmid; line 3, double digested product; line 4, PCR product of Lsa63. C. western blot of rLsa63 protein: (left to right) line 1, protein ladder; line 2, western blot of rLsa63.

    Journal: Iranian Red Crescent Medical Journal

    Article Title: Diagnostic Efficacy of Lsa63 Antigen for Human Leptospirosis

    doi: 10.5812/ircmj.14753

    Figure Lengend Snippet: Lsa63 PCR Product, Double digestion of pET28 a(+)-Lsa63 and Western Blot of rLsa63 Protein A. lane 1, Kb DNA size marker; line 2, Lsa63-specific PCR products. B. Double digestion of pET28a (+)-Lsa63 using NdeI/XhoI: (left to right) line 1, 1 Kb ladder; line 2, recombinant plasmid; line 3, double digested product; line 4, PCR product of Lsa63. C. western blot of rLsa63 protein: (left to right) line 1, protein ladder; line 2, western blot of rLsa63.

    Article Snippet: Using Pfu polymerase, the Lsa63 gene (LIC10314) was amplified (1614 bp) and then was cloned directly into an expression vector pET28a (+) at NdeI and XhoI restriction sites by Clontech cloning kit (Clontech, USA).

    Techniques: Polymerase Chain Reaction, Western Blot, Marker, Recombinant, Plasmid Preparation

    Schemes of CHL1-GFP/GUS Constructs. (A) CHL1 gene structure. Closed boxes represent exons containing coding regions, open boxes represent 5′ and 3′ untranslated sequences, and numbers indicate nucleotides from the start of translation. (B) HaeII reporter construct with ∼4.9 kb of CHL1 promoter sequence and exon 1 (up to amino acid 34 of CHL1) fused in frame at the HaeII site to the coding sequence of GFP or GUS . NOS indicates the 3′ termination sequence of the nopaline synthase gene. (C) XhoI reporter construct with the 4.9-kb CHL1 promoter and intragenic sequences up to the middle of exon 4 (up to amino acid 269 of CHL1) fused in frame to the coding sequence of GFP or GUS .

    Journal: The Plant Cell

    Article Title: The Arabidopsis Dual-Affinity Nitrate Transporter Gene AtNRT1.1 (CHL1) Is Activated and Functions in Nascent Organ Development during Vegetative and Reproductive Growth

    doi: 10.11054/TPC.010126

    Figure Lengend Snippet: Schemes of CHL1-GFP/GUS Constructs. (A) CHL1 gene structure. Closed boxes represent exons containing coding regions, open boxes represent 5′ and 3′ untranslated sequences, and numbers indicate nucleotides from the start of translation. (B) HaeII reporter construct with ∼4.9 kb of CHL1 promoter sequence and exon 1 (up to amino acid 34 of CHL1) fused in frame at the HaeII site to the coding sequence of GFP or GUS . NOS indicates the 3′ termination sequence of the nopaline synthase gene. (C) XhoI reporter construct with the 4.9-kb CHL1 promoter and intragenic sequences up to the middle of exon 4 (up to amino acid 269 of CHL1) fused in frame to the coding sequence of GFP or GUS .

    Article Snippet: CHL1-GUS constructs were made with translational fusions of the 4.9-kb HindIII-XhoI fragment and the 6.1-kb HindIII-SpeI fragment into the HindIII-SalI and HindIII-XbaI sites of the pBI101.2-GUS vector, respectively (Clontech, Palo Alto, CA).

    Techniques: Construct, Sequencing

    Generation of IL23R-CHR protein. A, Restriction sites are indicated in bold, the translated amino acid and Trx-6×His-DDDDKA tag are shown in the box; B, IL23R-CHR gene from human spleen cDNA library amplified by PCR; C, One pET32a/IL23R-CHR clone digested with NcoI and XhoI and analyzed by agrose gel electrophoresis (1%). MW = molecular weight markers, bp. D, Trx-IL23R-CHR induced by IPTG in E.Coli BL21 (DE3), SDS-PAGE (15%), protein bands were stained with coomassie brilliant blue R250 reagent. Lane 2, uninduced bacterial lysate; Lane 3, IPTG induced bacterial lysate. E, Trx-IL23R-CHR purified from BL21 (DE3) lysate and cleaved by enterokinase, Lane 5, purified Trx-IL23R-CHR; Lane 6, purified IL23R-CHR. F, Western blot using mouse mAbs against human IL23R (Lane 8). Lane 1, 4 and 7 in D, E, F: molecular weight markers, KD.

    Journal: PLoS ONE

    Article Title: Protection against Th17 Cells Differentiation by an Interleukin-23 Receptor Cytokine-Binding Homology Region

    doi: 10.1371/journal.pone.0045625

    Figure Lengend Snippet: Generation of IL23R-CHR protein. A, Restriction sites are indicated in bold, the translated amino acid and Trx-6×His-DDDDKA tag are shown in the box; B, IL23R-CHR gene from human spleen cDNA library amplified by PCR; C, One pET32a/IL23R-CHR clone digested with NcoI and XhoI and analyzed by agrose gel electrophoresis (1%). MW = molecular weight markers, bp. D, Trx-IL23R-CHR induced by IPTG in E.Coli BL21 (DE3), SDS-PAGE (15%), protein bands were stained with coomassie brilliant blue R250 reagent. Lane 2, uninduced bacterial lysate; Lane 3, IPTG induced bacterial lysate. E, Trx-IL23R-CHR purified from BL21 (DE3) lysate and cleaved by enterokinase, Lane 5, purified Trx-IL23R-CHR; Lane 6, purified IL23R-CHR. F, Western blot using mouse mAbs against human IL23R (Lane 8). Lane 1, 4 and 7 in D, E, F: molecular weight markers, KD.

    Article Snippet: After purification with Tianquick midi purification kit (Tiangen, China), the IL23R-CHR gene was digested with NcoI and XhoI (takara) and inserted into pET32a following the manufacturer’s instructions (Novagen, Germany).

    Techniques: cDNA Library Assay, Amplification, Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Molecular Weight, SDS Page, Staining, Purification, Western Blot

    Analysis of SMX Complex Formation throughout the Cell Cycle (A) Domain organization of human SLX1-SLX4, MUS81-EME1, and XPF-ERCC1, showing interactions between the SLX4 scaffold and the SLX1, MUS81, and XPF nuclease subunits. Interacting domains are denoted by double-headed arrows. Abbreviations for protein domains (top to bottom, left to right) are as follows: ERCC4, excision repair cross complementing 4; HhH, helix-hairpin-helix; WH, winged helix; UBZ, ubiquitin-binding zinc finger; MLR, MEI9 XPF interaction-like region; BTB, broad complex, tramtrack, and bric a brac; SIM, SUMO-interacting motif; SAP, C-terminal SAF-A/B, acinus, and PIAS; CCD, conserved C-terminal domain; SF2, superfamily 2; RING, really interesting new gene; GIY-YIG, conserved amino acids that form the catalytic motif. (B and C) Whole-cell extracts prepared from Flp-In T-REx 293 fibroblasts expressing FLAG SLX4 synchronized at G1/S (B) and G2/M (C) were centrifuged through 10%–45% sucrose gradients. Fractions were analyzed by western blotting for the indicated proteins. The positions of molecular weight markers are indicated. Boxed areas show the migration positions of SLX4-free MUS81-EME1 (fractions 7–9) and the SMX complex (fractions 11–14). Asterisks denote non-specific cross-reacting proteins. See also Figure S1 .

    Journal: Molecular Cell

    Article Title: The SMX DNA Repair Tri-nuclease

    doi: 10.1016/j.molcel.2017.01.031

    Figure Lengend Snippet: Analysis of SMX Complex Formation throughout the Cell Cycle (A) Domain organization of human SLX1-SLX4, MUS81-EME1, and XPF-ERCC1, showing interactions between the SLX4 scaffold and the SLX1, MUS81, and XPF nuclease subunits. Interacting domains are denoted by double-headed arrows. Abbreviations for protein domains (top to bottom, left to right) are as follows: ERCC4, excision repair cross complementing 4; HhH, helix-hairpin-helix; WH, winged helix; UBZ, ubiquitin-binding zinc finger; MLR, MEI9 XPF interaction-like region; BTB, broad complex, tramtrack, and bric a brac; SIM, SUMO-interacting motif; SAP, C-terminal SAF-A/B, acinus, and PIAS; CCD, conserved C-terminal domain; SF2, superfamily 2; RING, really interesting new gene; GIY-YIG, conserved amino acids that form the catalytic motif. (B and C) Whole-cell extracts prepared from Flp-In T-REx 293 fibroblasts expressing FLAG SLX4 synchronized at G1/S (B) and G2/M (C) were centrifuged through 10%–45% sucrose gradients. Fractions were analyzed by western blotting for the indicated proteins. The positions of molecular weight markers are indicated. Boxed areas show the migration positions of SLX4-free MUS81-EME1 (fractions 7–9) and the SMX complex (fractions 11–14). Asterisks denote non-specific cross-reacting proteins. See also Figure S1 .

    Article Snippet: The pFL_MUS81Δ86 -FLAG EME1 plasmid was created by inserting MUS81Δ86 into XhoI-linearized pFL_MUS81-FLAG EME1 using InFusion HD Cloning (Clontech) (note that XhoI digestion excises full-length MUS81 from pFL_MUS81-FLAG EME1).

    Techniques: Binding Assay, Expressing, Western Blot, Molecular Weight, Migration