xhoi endonuclease  (New England Biolabs)


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  • 99
    Name:
    Endonuclease V
    Description:
    Endonuclease V 250 units
    Catalog Number:
    m0305s
    Price:
    76
    Size:
    250 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs xhoi endonuclease
    Endonuclease V
    Endonuclease V 250 units
    https://www.bioz.com/result/xhoi endonuclease/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi endonuclease - by Bioz Stars, 2020-12
    99/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

    Methylation:

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

    Isolation:

    Article Title: The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI
    Article Snippet: .. The nicking endonuclease N. Bst NBI was originally isolated from the thermophilic bacterium B.stearothermophilus (NEB 928). .. Type IIs restriction endonucleases Ple I and Mly I were isolated from the mesophilic bacteria Pseudomonas lemoignei and Micrococcus lylae , respectively.

    Purification:

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
    Article Snippet: .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of restriction endonuclease BsrD I (New England Biolabs, Ipswich, MA, USA). ..

    Inhibition:

    Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter
    Article Snippet: .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the AatII restriction endonuclease (NEB). .. Electrophoretic mobility shift assays Vectors pCRE-uno and pCRE-zero were digested with PciI and BmtI (both NEB) to produce the CRE-less 4228 bp fragment and the 180 bp fragment with one (pCRE-uno) or no CRE (pCRE-zero) and cleaned up.

    Polymerase Chain Reaction:

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
    Article Snippet: .. 5) Detection of restriction fragment length polymorphism in the metalloproteinase-1 regions Ten micrograms of purified PCR sample was digested at 65℃ for 16 hours with 10 U of restriction endonuclease BsrD I (New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Efficient genome editing in hematopoietic stem cells with helper-dependent Ad5/35 vectors expressing site-specific endonucleases under microRNA regulation
    Article Snippet: .. PCR products were hybridized and treated with 2.5 units of T7E1 endonuclease (NEB, Ipswich, MA). .. Digested PCR products were resolved by 10% Tris-borate EDTA buffer (TBE) polyacrylamide gel electrophoresis (Biorad) and stained with ethidium bromide.

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: .. 2) For HpaII digestion of genomic DNA, followed by SAMHD1 promoter-specific PCR, genomic DNA (1 μg) was digested with 10 units of HpaII endonuclease (New England Biolabs) for 16 h at 37 °C. .. The enzyme was heat-inactivated, and DNA was purified using DNA Clean and ConcentratorTM -5.

    Plasmid Preparation:

    Article Title: Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoter
    Article Snippet: .. The presence of 5-mC/5-hmC/5-fC/5-caC in the covalently closed vector DNA was verified by the inhibition of cleavage by the AatII restriction endonuclease (NEB). .. Electrophoretic mobility shift assays Vectors pCRE-uno and pCRE-zero were digested with PciI and BmtI (both NEB) to produce the CRE-less 4228 bp fragment and the 180 bp fragment with one (pCRE-uno) or no CRE (pCRE-zero) and cleaned up.

    Article Title: Promoter Methylation Regulates SAMHD1 Gene Expression in Human CD4+ T Cells *
    Article Snippet: .. Complete methylation of the pGL4-SAMHD1-luc plasmid was confirmed by digestion with the methylation-sensitive endonuclease HpaII (New England Biolabs), followed by agarose gel electrophoresis. .. Two methods were employed to confirm the methylation status of the SAMHD1 promoter in CD4+ T cells.

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  • 99
    New England Biolabs restriction endonuclease xhoi
    Production of joint molecules used to test the branch migration activity of proteins. (A) Joint molecules with a 3′ displaced ssDNA tail are produced by RAD51 using gapped DNA and <t>pBS</t> II K (+) dsDNA linearized with <t>XhoI.</t> (B) Joint molecules with
    Restriction Endonuclease Xhoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease xhoi/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease xhoi - by Bioz Stars, 2020-12
    99/100 stars
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    99
    New England Biolabs restriction endonucleases xhoi
    a Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs. 1 —Sample, isolated from IBs, b Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs treated with restriction endonuclease <t>XhoI</t> and c HindIII. 1 —Non-treated sample; 2(b) —Sample treated with XhoI; 2(c) —sample treated with HindIII; d Enzymatic digestion of nucleic acids isolated from purified hIFNγ IBs 1 —non treated sample; 2 —sample treated with mixture of <t>RNase</t> A and RNase T 1 ; 3 —sample treated with DNase I; 4 —sample treated with Proteinase K; M —Molecular weight marker, bp
    Restriction Endonucleases Xhoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases xhoi/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases xhoi - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    93
    New England Biolabs restriction endonucleases
    a Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs. 1 —Sample, isolated from IBs, b Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs treated with restriction endonuclease <t>XhoI</t> and c HindIII. 1 —Non-treated sample; 2(b) —Sample treated with XhoI; 2(c) —sample treated with HindIII; d Enzymatic digestion of nucleic acids isolated from purified hIFNγ IBs 1 —non treated sample; 2 —sample treated with mixture of <t>RNase</t> A and RNase T 1 ; 3 —sample treated with DNase I; 4 —sample treated with Proteinase K; M —Molecular weight marker, bp
    Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases/product/New England Biolabs
    Average 93 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases - by Bioz Stars, 2020-12
    93/100 stars
      Buy from Supplier

    Image Search Results


    Production of joint molecules used to test the branch migration activity of proteins. (A) Joint molecules with a 3′ displaced ssDNA tail are produced by RAD51 using gapped DNA and pBS II K (+) dsDNA linearized with XhoI. (B) Joint molecules with

    Journal: Methods (San Diego, Calif.)

    Article Title: Analyzing the Branch Migration Activities of Eukaryotic Proteins

    doi: 10.1016/j.ymeth.2010.02.010

    Figure Lengend Snippet: Production of joint molecules used to test the branch migration activity of proteins. (A) Joint molecules with a 3′ displaced ssDNA tail are produced by RAD51 using gapped DNA and pBS II K (+) dsDNA linearized with XhoI. (B) Joint molecules with

    Article Snippet: To prepare the linear dsDNA fragment, perform a digest of pBS II K (+) plasmid DNA first with the restriction endonuclease XhoI, and then with AlwNI (New England Biolabs).

    Techniques: Migration, Activity Assay, Produced

    The scheme used to produce gapped DNA. i) pBS II K (+) plasmid DNA is digested with XhoI and AlwNI. ii) The large DNA fragment from this digest is purified by gel electrophoresis in agarose gels, and then iii) annealed to circular ssDNA to generate gapped

    Journal: Methods (San Diego, Calif.)

    Article Title: Analyzing the Branch Migration Activities of Eukaryotic Proteins

    doi: 10.1016/j.ymeth.2010.02.010

    Figure Lengend Snippet: The scheme used to produce gapped DNA. i) pBS II K (+) plasmid DNA is digested with XhoI and AlwNI. ii) The large DNA fragment from this digest is purified by gel electrophoresis in agarose gels, and then iii) annealed to circular ssDNA to generate gapped

    Article Snippet: To prepare the linear dsDNA fragment, perform a digest of pBS II K (+) plasmid DNA first with the restriction endonuclease XhoI, and then with AlwNI (New England Biolabs).

    Techniques: Plasmid Preparation, Purification, Nucleic Acid Electrophoresis

    Illustration of the 0.8% agarose gel used to purify the large (2065 bp) dsDNA fragment of pBS II K (+) following digestion with XhoI and AlwNI. After electrophoresis, lanes A, C, and E are excised from the gel and stained with ethidium bromide (dashed

    Journal: Methods (San Diego, Calif.)

    Article Title: Analyzing the Branch Migration Activities of Eukaryotic Proteins

    doi: 10.1016/j.ymeth.2010.02.010

    Figure Lengend Snippet: Illustration of the 0.8% agarose gel used to purify the large (2065 bp) dsDNA fragment of pBS II K (+) following digestion with XhoI and AlwNI. After electrophoresis, lanes A, C, and E are excised from the gel and stained with ethidium bromide (dashed

    Article Snippet: To prepare the linear dsDNA fragment, perform a digest of pBS II K (+) plasmid DNA first with the restriction endonuclease XhoI, and then with AlwNI (New England Biolabs).

    Techniques: Agarose Gel Electrophoresis, Electrophoresis, Staining

    a Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs. 1 —Sample, isolated from IBs, b Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs treated with restriction endonuclease XhoI and c HindIII. 1 —Non-treated sample; 2(b) —Sample treated with XhoI; 2(c) —sample treated with HindIII; d Enzymatic digestion of nucleic acids isolated from purified hIFNγ IBs 1 —non treated sample; 2 —sample treated with mixture of RNase A and RNase T 1 ; 3 —sample treated with DNase I; 4 —sample treated with Proteinase K; M —Molecular weight marker, bp

    Journal: Microbial Cell Factories

    Article Title: Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma

    doi: 10.1186/s12934-020-01400-6

    Figure Lengend Snippet: a Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs. 1 —Sample, isolated from IBs, b Agarose gel-electrophoresis of nucleic acids isolated from purified hIFNγ IBs treated with restriction endonuclease XhoI and c HindIII. 1 —Non-treated sample; 2(b) —Sample treated with XhoI; 2(c) —sample treated with HindIII; d Enzymatic digestion of nucleic acids isolated from purified hIFNγ IBs 1 —non treated sample; 2 —sample treated with mixture of RNase A and RNase T 1 ; 3 —sample treated with DNase I; 4 —sample treated with Proteinase K; M —Molecular weight marker, bp

    Article Snippet: Enzymatic treatment of nucleic acids isolated from IBsNucleic acids extracted from purified IBs were treated with RNAse A (Thermo Scientific™, 10 mg/ml), RNase T1 (Thermo Scientific™, 1000 U/ml), DNAse I, restriction endonucleases XhoI and HindIII (New England BioLabs) and Proteinase K (Roche, 10 μg/ml) following the manufacturers’ protocols.

    Techniques: Agarose Gel Electrophoresis, Isolation, Purification, Molecular Weight, Marker