xf ksr  (Thermo Fisher)


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  • 99
    Name:
    Image IT LIVE Lysosomal and Nuclear Labeling Kit Counterstains For GFP Expressing Cells
    Description:
    The Image iT LIVE Lysosomal and Nuclear Labeling Kit provides two stains red fluorescent LysoTracker Red DND 99 dye and blue fluorescent Hoechst 33342 dye for highly selective staining of lysosomes and the nucleus respectively in live green fluorescent protein GFP transfected cells When used according to the sample protocol cell permeant LysoTracker Red DND 99 provides highly selective lysosomal staining with minimal background Hoechst 33342 dye a cell permeant nucleic acid stain that is selective for DNA and spectrally similar to DAPI is UV excitable and emits blue fluorescence when bound to DNA This dye should not interfere with GFP fluorescence and is retained after fixation and permeabilization
    Catalog Number:
    I34202
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|Cell-Based Reporter Assays|Cellular Imaging|Enzyme Reporter Assays|Fluorescent Protein (e.g. GFP) Assays|Fluorescent Protein Assays (GFP)|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Lysosomes|Nucleus, Nucleoli & Nuclear Envelope|Protein Assays and Analysis|Protein Biology|Cell Structure
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    Structured Review

    Thermo Fisher xf ksr
    The Image iT LIVE Lysosomal and Nuclear Labeling Kit provides two stains red fluorescent LysoTracker Red DND 99 dye and blue fluorescent Hoechst 33342 dye for highly selective staining of lysosomes and the nucleus respectively in live green fluorescent protein GFP transfected cells When used according to the sample protocol cell permeant LysoTracker Red DND 99 provides highly selective lysosomal staining with minimal background Hoechst 33342 dye a cell permeant nucleic acid stain that is selective for DNA and spectrally similar to DAPI is UV excitable and emits blue fluorescence when bound to DNA This dye should not interfere with GFP fluorescence and is retained after fixation and permeabilization
    https://www.bioz.com/result/xf ksr/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xf ksr - by Bioz Stars, 2021-04
    99/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: SERTAD1 PLAYS AN ESSENTIAL ROLE IN DEVELOPMENTAL AND PATHOLOGICAL NEURON DEATH
    Article Snippet: Apoptotic cells exhibit strong, nuclear red fluorescence. .. To visualize GFP positive cells, sections were then immunostained with rabbit anti GFP antibody (1:1000, Invitrogen, Carlsbad, CA) in 3% nonimmune goat serum overnight at 4°C, followed by secondary labeling with goat anti-rabbit antibody (1:4000, Alexa Fluor 488, Molecular Probes, CA) for 1 hr. .. Neuronal PC12 cells, sympathetic neurons or cortical neurons were transfected with either pCMS-Sertad1-EGFP, pCMS-EGFP, pSIREN-Sertad1-shRNA, pSIREN-Luc-shRNA, or a Random pSIREN-ZsGreen, and then 48 hrs later deprived of NGF (in case of neuronal PC12 cells and sympathetic neurons) or treated with 1.25 μM dodecamer Aβ (in case of cortical neurons).

    Software:

    Article Title: C3a receptor blockade protects podocytes from injury in diabetic nephropathy
    Article Snippet: The percentage of podocytes with an altered mitochondrial pattern, in terms of fragmentation and perinuclear redistribution, on total cells per field was assessed by staining with MitoTracker in 15 random fields per sample. .. Mitochondrial morphology was evaluated in 15 random fields per sample using the MiNA tool set ( ) in the Fiji software and normalized for the number of Hoechst-positive cells. .. The quantification of JC-1 red and green areas was performed in 15 random fields per sample by using ImageJ 1.40g software, and mitochondrial polarization was expressed as the ratio between red and green fluorescent areas and normalized for the number of Hoechst-positive cells.

    Article Title: C3a receptor blockade protects podocytes from injury in diabetic nephropathy
    Article Snippet: Mitochondrial morphology was evaluated in 15 random fields per sample using the MiNA tool set ( ) in the Fiji software and normalized for the number of Hoechst-positive cells. .. The quantification of JC-1 red and green areas was performed in 15 random fields per sample by using ImageJ 1.40g software, and mitochondrial polarization was expressed as the ratio between red and green fluorescent areas and normalized for the number of Hoechst-positive cells. .. Statistics The sample size for the in vivo studies was estimated to be at least 4 mice per group to enable the detection of a mean of paired difference in albuminuria of 30 μg/d with an estimated standard deviation of 10 (α = 0.05, paired t test, 2-tailed test) with an 80% power, based on our previous published data ( ).

    Live Cell Imaging:

    Article Title:
    Article Snippet: .. On the day of live cell imaging, the green fluorescent dye ThT (UPR-indicative dye) (Sigma-Aldrich) was freshly prepared as previously described , and the blue fluorescent live cell nuclear dye Hoechst 33342 (counterstain dye) (Thermo Fisher Scientific) was freshly prepared at a final concertation of 5 μg/ml. .. The staining with ThT was for 1 hour, and this was followed by substituting the culture media (containing test compounds and ThT) with PBS containing Hoechst 33342 for 15 minutes in a CO2 incubator.

    Fluorescence:

    Article Title: Age and axon-specific forms of cortical remyelination by divergent populations of NG2-glia
    Article Snippet: .. For two-photon fluorescence microscopy, 775nm was used to excite Hoechst nuclear dye, 920nm was used to excite mEGFP, and 1040nm was used to excite tdTomato. ..

    Microscopy:

    Article Title: Age and axon-specific forms of cortical remyelination by divergent populations of NG2-glia
    Article Snippet: .. For two-photon fluorescence microscopy, 775nm was used to excite Hoechst nuclear dye, 920nm was used to excite mEGFP, and 1040nm was used to excite tdTomato. ..

    Staining:

    Article Title: Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence
    Article Snippet: Upon termination of all trials, cells were fixed in formaldehyde (4% for 15 min), incubated with Hoechst stain (0.0005% for 15 min) (ThermoFisher, Inc., Waltham, MA), then imaged at 4X on the CellInsight CX5 High Content Screening (HCS) Platform. .. The machine was allowed to find optimal focus for each Hoechst stain image. .. One color channel was permitted such that images produced showed live nuclei in white and dead cells in black.

    other:

    Article Title: Excitable axonal domains adapt to sensory deprivation in the olfactory system
    Article Snippet: The OB is a highly stratified structure whose layers are clearly delimited using the nuclear label Hoechst ( ).

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  • 99
    Thermo Fisher dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium
    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with <t>Dulbecco's</t> modified <t>Eagle's</t> medium <t>KSR‐XF</t> alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.
    Dulbecco S Modified Eagle S Medium Dmem Ksr Xf Ksr Xf Knockout Dmem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium - by Bioz Stars, 2021-04
    99/100 stars
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    94
    Thermo Fisher ksr xf
    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with <t>Dulbecco's</t> modified <t>Eagle's</t> medium <t>KSR‐XF</t> alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.
    Ksr Xf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ksr xf/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ksr xf - by Bioz Stars, 2021-04
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    95
    Thermo Fisher xf ksr human embryonic stem cell hesc medium
    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with <t>Dulbecco's</t> modified <t>Eagle's</t> medium <t>KSR‐XF</t> alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.
    Xf Ksr Human Embryonic Stem Cell Hesc Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xf ksr human embryonic stem cell hesc medium/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xf ksr human embryonic stem cell hesc medium - by Bioz Stars, 2021-04
    95/100 stars
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    Image Search Results


    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with Dulbecco's modified Eagle's medium KSR‐XF alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with Dulbecco's modified Eagle's medium KSR‐XF alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Modification, Immunocytochemistry, Marker, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay

    Stage 2: Generation of a mixed RPE population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Dulbecco's modified Eagle's medium (DMEM) KSR‐XF alone (Control) or Induction Medium 3 (ActA) for 19 days. Quantitative polymerase chain reaction was used to measure expression of a panel of RPE markers. RPE generated by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). (B): Representative images showing immunocytochemistry for CRALBP and MERTK in cells derived by directed differentiation and cultured as in panel A. Dotted squares indicate the region that is magnified in the panels below. RPE generated by spontaneous differentiation were used for comparison. Images are captured at ×10 magnification. (C): Quantification of CRALBP immunocytochemistry at day 28 in which cells during stage 2 were treated with DMEM KSR‐XF (Control) or Induction Medium 3 (ActA) for 3 days, 5 days, 10 days, or 19 days. For fewer than 19‐day ActA treatments, DMEM KSR‐XF was used for the remainder of the 19‐day period. RPE derived by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). Abbreviations: ActA, Activin A; d, days; RPE, retinal pigment epithelium; Sp. RPE, spontaneous differentiation retinal pigment epithelium.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 2: Generation of a mixed RPE population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Dulbecco's modified Eagle's medium (DMEM) KSR‐XF alone (Control) or Induction Medium 3 (ActA) for 19 days. Quantitative polymerase chain reaction was used to measure expression of a panel of RPE markers. RPE generated by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). (B): Representative images showing immunocytochemistry for CRALBP and MERTK in cells derived by directed differentiation and cultured as in panel A. Dotted squares indicate the region that is magnified in the panels below. RPE generated by spontaneous differentiation were used for comparison. Images are captured at ×10 magnification. (C): Quantification of CRALBP immunocytochemistry at day 28 in which cells during stage 2 were treated with DMEM KSR‐XF (Control) or Induction Medium 3 (ActA) for 3 days, 5 days, 10 days, or 19 days. For fewer than 19‐day ActA treatments, DMEM KSR‐XF was used for the remainder of the 19‐day period. RPE derived by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). Abbreviations: ActA, Activin A; d, days; RPE, retinal pigment epithelium; Sp. RPE, spontaneous differentiation retinal pigment epithelium.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Generated, Immunocytochemistry, Derivative Assay, Cell Culture

    Stage 3: Generation of a homogenous and functional retinal pigment epithelium (RPE) population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Induction Medium 3 for a period of 19 days. At day 28, cells were replated in Dulbecco's modified Eagle's medium KSR‐XF and cultured for a period of 14 days. Representative images showing immunocytochemistry for indicated RPE markers are shown. Images are captured at ×10 magnification. (B): Cells cultured as in panel A were further dissociated and replated on transwells and cultured for a period of 10 weeks. A representative image showing en face immunocytochemistry for ZO‐1 is shown. Images are captured at ×20 magnification. (C): Spent medium from transwells described in panel B was collected from the top and bottom chambers and quantified for vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF) concentration. The ratio of [VEGF]:[PEDF] was quantified in media from the two compartments ( n = 3, ±SD). (D): Representative confocal images showing phagocytosis of fluorescent bead (red) by RPE. ZO‐1 immunocytochemistry (green) shows the cell edge, and the presence of bead within the cell boundary indicates internalization by phagocytosis. Images are captured at ×63 magnification. Abbreviations: PEDF, pigment epithelium‐derived factor; VEGF, vascular endothelial growth factor.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 3: Generation of a homogenous and functional retinal pigment epithelium (RPE) population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Induction Medium 3 for a period of 19 days. At day 28, cells were replated in Dulbecco's modified Eagle's medium KSR‐XF and cultured for a period of 14 days. Representative images showing immunocytochemistry for indicated RPE markers are shown. Images are captured at ×10 magnification. (B): Cells cultured as in panel A were further dissociated and replated on transwells and cultured for a period of 10 weeks. A representative image showing en face immunocytochemistry for ZO‐1 is shown. Images are captured at ×20 magnification. (C): Spent medium from transwells described in panel B was collected from the top and bottom chambers and quantified for vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF) concentration. The ratio of [VEGF]:[PEDF] was quantified in media from the two compartments ( n = 3, ±SD). (D): Representative confocal images showing phagocytosis of fluorescent bead (red) by RPE. ZO‐1 immunocytochemistry (green) shows the cell edge, and the presence of bead within the cell boundary indicates internalization by phagocytosis. Images are captured at ×63 magnification. Abbreviations: PEDF, pigment epithelium‐derived factor; VEGF, vascular endothelial growth factor.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Functional Assay, Modification, Cell Culture, Immunocytochemistry, Derivative Assay, Concentration Assay