xbai (Thermo Fisher)
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Name:
XbaI 10 U µL
Description:
5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
Catalog Number:
er0681
Price:
None
Category:
Proteins Enzymes Peptides
Applications:
Cloning|Restriction Enzyme Cloning
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Structured Review
Thermo Fisher
xbai
5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
https://www.bioz.com/result/xbai/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
https://www.bioz.com/result/xbai/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
xbai - by Bioz Stars,
2021-03
86/100 stars
Images
1) Product Images from "Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)"
Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
Journal: 3 Biotech
doi: 10.1007/s13205-018-1552-0
Figure Legend Snippet: Southern blot analysis of the four STS markers- CtR-431, CtR-594., CtR-496, and AFLP-376. Restriction enzymes used are EcoRI, HinDIII and XbaI . M, molecular weight marker; R resistant genotype Punjab Lal; S susceptible genotype Arka Lohit. Hybridization
Techniques Used: Southern Blot, Molecular Weight, Marker, Hybridization
Related Articles
Plasmid Preparation:Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Article Snippet: The product was digested with XbaI and SpeI (part of the amplified pBluescript backbone, compatible with XbaI) and cloned into the XbaI site of pG-640-CAT/EP2. pG-1240-CAT/EP2 (1240F ): the first 1240 bp of PAG1 were amplified by PCR with the primers PAG1down2 and 1240up on the genomic DNA clone λPAG2-711 and the product cloned into pCR®2.1-TOPO (Invitrogen). .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and Article Title: Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells Article Snippet: Plasmid Construction The full-length coding sequence of human IL-7 ( ) was commercially synthesized in pUC57 by ShineGene (Shanghai, China). .. After digestion with SalI and Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis Article Snippet: Construction of the recombinant pcDNA3.1 (+)/HspX-PPE44-EsxV vector The optimized HspX-PPE44-EsxV fusion segment was synthesized in the pGH vector by Generay Company (China) in BamHI and XbaI restriction sites at the 5´and 3´ ends. .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and Article Title: Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance Article Snippet: 4.2. pP15A, kanaR Vector Confirmation PCR amplification of the M13K07 plasmid DNA fragment containing P15A origin of replication and kanaR gene resulted in a 2592-bp band on agarose gel electrophoresis ( ). .. The plasmid DNA purified from a single positive clone containing self-ligated pP15A, kanaR was confirmed by both single and double digestion reactions with BamHI and Clone Assay:Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Article Snippet: The product was digested with XbaI and SpeI (part of the amplified pBluescript backbone, compatible with XbaI) and cloned into the XbaI site of pG-640-CAT/EP2. pG-1240-CAT/EP2 (1240F ): the first 1240 bp of PAG1 were amplified by PCR with the primers PAG1down2 and 1240up on the genomic DNA clone λPAG2-711 and the product cloned into pCR®2.1-TOPO (Invitrogen). .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and Article Title: The development and characterization of SDF1α-elastin-like-peptide nanoparticles for wound healing Article Snippet: SDF1 was fused to ELP via these 2 restriction sites. .. The NdeI and Amplification:Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Article Snippet: The product was digested with XbaI and SpeI (part of the amplified pBluescript backbone, compatible with XbaI) and cloned into the XbaI site of pG-640-CAT/EP2. pG-1240-CAT/EP2 (1240F ): the first 1240 bp of PAG1 were amplified by PCR with the primers PAG1down2 and 1240up on the genomic DNA clone λPAG2-711 and the product cloned into pCR®2.1-TOPO (Invitrogen). .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and Polymerase Chain Reaction:Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Article Snippet: The product was digested with XbaI and SpeI (part of the amplified pBluescript backbone, compatible with XbaI) and cloned into the XbaI site of pG-640-CAT/EP2. pG-1240-CAT/EP2 (1240F ): the first 1240 bp of PAG1 were amplified by PCR with the primers PAG1down2 and 1240up on the genomic DNA clone λPAG2-711 and the product cloned into pCR®2.1-TOPO (Invitrogen). .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and Sequencing:Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Article Snippet: The product was digested with XbaI and SpeI (part of the amplified pBluescript backbone, compatible with XbaI) and cloned into the XbaI site of pG-640-CAT/EP2. pG-1240-CAT/EP2 (1240F ): the first 1240 bp of PAG1 were amplified by PCR with the primers PAG1down2 and 1240up on the genomic DNA clone λPAG2-711 and the product cloned into pCR®2.1-TOPO (Invitrogen). .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and Pulsed-Field Gel:Article Title: Molecular Characterization of Carbapenem Resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae Isolated from Lebanon Article Snippet: .. Pulsed-field gel electrophoresis (PFGE) PFGE fingerprinting was performed using the Agarose Gel Electrophoresis:Article Title: Molecular Characterization of Carbapenem Resistant Klebsiella pneumoniae and Klebsiella quasipneumoniae Isolated from Lebanon Article Snippet: .. Pulsed-field gel electrophoresis (PFGE) PFGE fingerprinting was performed using the Gel Extraction:Article Title: Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells Article Snippet: Plasmid Construction The full-length coding sequence of human IL-7 ( ) was commercially synthesized in pUC57 by ShineGene (Shanghai, China). .. After digestion with SalI and Expressing:Article Title: Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells Article Snippet: Plasmid Construction The full-length coding sequence of human IL-7 ( ) was commercially synthesized in pUC57 by ShineGene (Shanghai, China). .. After digestion with SalI and Generated:Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis Article Snippet: Construction of the recombinant pcDNA3.1 (+)/HspX-PPE44-EsxV vector The optimized HspX-PPE44-EsxV fusion segment was synthesized in the pGH vector by Generay Company (China) in BamHI and XbaI restriction sites at the 5´and 3´ ends. .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and Purification:Article Title: Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance Article Snippet: 4.2. pP15A, kanaR Vector Confirmation PCR amplification of the M13K07 plasmid DNA fragment containing P15A origin of replication and kanaR gene resulted in a 2592-bp band on agarose gel electrophoresis ( ). .. The plasmid DNA purified from a single positive clone containing self-ligated pP15A, kanaR was confirmed by both single and double digestion reactions with BamHI and DNA Ligation:Article Title: The development and characterization of SDF1α-elastin-like-peptide nanoparticles for wound healing Article Snippet: SDF1 was fused to ELP via these 2 restriction sites. .. The NdeI and Mutagenesis:Article Title: Mutation of the histidin residue of the DRH motif in vasopressin V2 receptor expression and function Article Snippet: The PCR products were confirmed by staining of the agarose gel with ethidium bromide using a UV transilluminator, and the obtained DNA bands were detected. .. The products containing the desired mutation were digested by BamHI, EcoRI and |
