xbai (New England Biolabs)


Name:
XbaI
Description:
XbaI 15 000 units
Catalog Number:
r0145l
Price:
290
Category:
Restriction Enzymes
Size:
15 000 units
|
Buy from Supplier |
Structured Review

XbaI 15 000 units
https://www.bioz.com/result/xbai/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A"
Article Title: Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A
Journal: mBio
doi: 10.1128/mBio.01298-18

Figure Legend Snippet: Generation of a trxA deletion mutant. A schematic representation of the WT A. baumannii Ci79 and Δ trxA mutant genome surrounding the trxA locus with predicted fragment sizes detected by probe following either HindIII (white arrowhead) or XbaI (black arrowhead) digestion as well as probe target region (dotted box) (A). Southern blot (B) and Western blot (C) analyses of genomic and protein extracts, respectively, from WT Ci79, ΔtrxA , and ΔtrxA c A. baumannii were subsequently performed. PCR amplification of the trxA gene locus was performed using DNA from WT Ci79, ΔtrxA , and ΔtrxA c A. baumannii isolates. The PCR amplicons were subsequently digested with SalI and subjected to agarose gel electrophoresis (C). Total bacterial proteins were separated by electrophoresis and visualized in a Coomassie blue-stained polyacrylamide gel (D, left panel) or probed with anti-TrxA antibody to detect TrxA expression (D, right panel). Molecular marker sizes for DNA (bp; B and C) and protein (kDa; D) are provided.
Techniques Used: Mutagenesis, Southern Blot, Western Blot, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Electrophoresis, Staining, Expressing, Marker
2) Product Images from "Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria"
Article Title: Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria
Journal: Korean Journal for Food Science of Animal Resources
doi: 10.5851/kosfa.2018.e17
![... (CCC type); 3: pTG262 ( luc + ) (EcoRI-XbaI). Restriction of pTG262 ( luc + ) (A) and physical genetic map of pTG262::pFI2576 rep ( luc + ) (B). M: HyperLadder™ 1 kb (Bioline Reagents Ltd.); 1: pTG262 [CCC (covalently closed circular) type]; 2: pTG262 ( luc + ) (CCC type); 3: pTG262 ( luc + ) (EcoRI-XbaI).](https://storage.googleapis.com/bioz_article_images/PMC6131385/kosfa-38-4-816-g2.jpg)
Figure Legend Snippet: Restriction of pTG262 ( luc + ) (A) and physical genetic map of pTG262::pFI2576 rep ( luc + ) (B). M: HyperLadder™ 1 kb (Bioline Reagents Ltd.); 1: pTG262 [CCC (covalently closed circular) type]; 2: pTG262 ( luc + ) (CCC type); 3: pTG262 ( luc + ) (EcoRI-XbaI).
Techniques Used: Countercurrent Chromatography
Related Articles
Agarose Gel Electrophoresis:Article Title: Antimicrobial Resistance, Virulence Profiles and Molecular Subtypes of Salmonella enterica Serovars Typhi and Paratyphi A Blood Isolates from Kolkata, India during 2009-2013 Article Snippet: .. The DNA plugs were digested with 40 U of Article Title: Generation and Characterization of a Transgenic Pig Carrying a DsRed-Monomer Reporter Gene Article Snippet: A Southern blot analysis was performed to confirm the transgene. .. The DNA were digested using Article Title: Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A Article Snippet: Genetic manipulation was confirmed by Southern blot analysis. .. Genomic DNAs from Ci79, Δ trxA , and Δ trxA c strains were isolated using the GeneJET genomic DNA purification kit (Thermo Scientific, Rockford, IL), digested with Article Title: Broad Meloidogyne Resistance in Potato Based on RNA Interference of Effector Gene 16D10 Article Snippet: .. For each plant line, 15 µg DNA was digested with 50 U Size-exclusion Chromatography:Article Title: Antimicrobial Resistance, Virulence Profiles and Molecular Subtypes of Salmonella enterica Serovars Typhi and Paratyphi A Blood Isolates from Kolkata, India during 2009-2013 Article Snippet: .. The DNA plugs were digested with 40 U of Staining:Article Title: Antimicrobial Resistance, Virulence Profiles and Molecular Subtypes of Salmonella enterica Serovars Typhi and Paratyphi A Blood Isolates from Kolkata, India during 2009-2013 Article Snippet: .. The DNA plugs were digested with 40 U of Diffusion-based Assay:Article Title: Generation and Characterization of a Transgenic Pig Carrying a DsRed-Monomer Reporter Gene Article Snippet: A Southern blot analysis was performed to confirm the transgene. .. The DNA were digested using Pulsed-Field Gel:Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain Article Snippet: .. Pulsed-field gel electrophoresis (PFGE) was performed using the Isolation:Article Title: Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A Article Snippet: Genetic manipulation was confirmed by Southern blot analysis. .. Genomic DNAs from Ci79, Δ trxA , and Δ trxA c strains were isolated using the GeneJET genomic DNA purification kit (Thermo Scientific, Rockford, IL), digested with DNA Purification:Article Title: Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A Article Snippet: Genetic manipulation was confirmed by Southern blot analysis. .. Genomic DNAs from Ci79, Δ trxA , and Δ trxA c strains were isolated using the GeneJET genomic DNA purification kit (Thermo Scientific, Rockford, IL), digested with Polymerase Chain Reaction:Article Title: A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism Article Snippet: PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the following cycling parameters: 30 s of initial incubation at 98°C, 35 cycles at 98°C for 10 s and at 72°C for 2 min, and a final elongation step of 2 min at 72°C. .. The PCR product was purified using QIAquick Gel Extraction Kit (Qiagen), digested with Hind III (NEB) and Purification:Article Title: A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism Article Snippet: PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the following cycling parameters: 30 s of initial incubation at 98°C, 35 cycles at 98°C for 10 s and at 72°C for 2 min, and a final elongation step of 2 min at 72°C. .. The PCR product was purified using QIAquick Gel Extraction Kit (Qiagen), digested with Hind III (NEB) and Gel Extraction:Article Title: A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism Article Snippet: PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the following cycling parameters: 30 s of initial incubation at 98°C, 35 cycles at 98°C for 10 s and at 72°C for 2 min, and a final elongation step of 2 min at 72°C. .. The PCR product was purified using QIAquick Gel Extraction Kit (Qiagen), digested with Hind III (NEB) and Clone Assay:Article Title: A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism Article Snippet: PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the following cycling parameters: 30 s of initial incubation at 98°C, 35 cycles at 98°C for 10 s and at 72°C for 2 min, and a final elongation step of 2 min at 72°C. .. The PCR product was purified using QIAquick Gel Extraction Kit (Qiagen), digested with Hind III (NEB) and Plasmid Preparation:Article Title: A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism Article Snippet: PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with the following cycling parameters: 30 s of initial incubation at 98°C, 35 cycles at 98°C for 10 s and at 72°C for 2 min, and a final elongation step of 2 min at 72°C. .. The PCR product was purified using QIAquick Gel Extraction Kit (Qiagen), digested with Hind III (NEB) and Article Title: Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria Article Snippet: PCR product was digested with SalI restriction endonuclease (New England Biolabs, Hitchin, UK) and inserted into pUC19 cloning vector digested with the same restriction enzyme. .. A luciferase gene (luc+ ) was liberated with Luciferase:Article Title: Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria Article Snippet: PCR product was digested with SalI restriction endonuclease (New England Biolabs, Hitchin, UK) and inserted into pUC19 cloning vector digested with the same restriction enzyme. .. A luciferase gene (luc+ ) was liberated with Recombinant:Article Title: Construction of a Bioluminescent Labelling Plasmid Vector for Bifidobacteria Article Snippet: PCR product was digested with SalI restriction endonuclease (New England Biolabs, Hitchin, UK) and inserted into pUC19 cloning vector digested with the same restriction enzyme. .. A luciferase gene (luc+ ) was liberated with |