xbai restriction endonuclease  (Thermo Fisher)


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    Name:
    Endonuclease V
    Description:
    Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
    Catalog Number:
    en0141
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Genotyping & Genomic Profiling|Rare & Low-Frequency Mutation Analysis|Gene Expression Analysis & Genotyping
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    Structured Review

    Thermo Fisher xbai restriction endonuclease
    Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
    https://www.bioz.com/result/xbai restriction endonuclease/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai restriction endonuclease - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Diversity of Green-Like and Red-Like Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes (cbbL) in Differently Managed Agricultural Soils
    Article Snippet: Each 10-μl digestion reaction mixture consisted of 2 μl of purified plasmid, 1 μl of buffer O+ (Fermentas GmbH), and 2 U of EcoRI (Fermentas GmbH) and was incubated at 37°C for 2 h. Clones which harbored a correctly sized red-like cbbL insert were screened by restriction fragment length polymorphism (RFLP). .. Ten microliters of each PCR product was hydrolyzed with 2 U of the restriction endonuclease BbvI (MBI Fermentas). .. Restriction fragments were analyzed in 3.5% (wt/vol) agarose gels (PeqLab) and visualized as described previously.

    Article Title: TILLING to detect induced mutations in soybean
    Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Genomic DNA restriction endonuclease digestion followed by high-throughput TILLING The gmrhg4 PCR product was amplified from the Forrest background and cloned using the pCR 4-TOPO TA kit (Invitrogen, Carlsbad, CA). .. Both strands of several clones were sequenced to generate consensus sequences for gmrhg4 and the homeolog/paralog.

    Incubation:

    Article Title: Multiplex quantitative analysis of microRNA expression via exponential isothermal amplification and conformation-sensitive DNA separation
    Article Snippet: The first 10 uL of extension reaction was used as the cleavage reaction and was added with 5 U of Nb.Bpu10I nicking endonuclease and 10 uL of 1× Buffer R. The remaining 10 uL was added with 10 uL of 1× buffer R and used as the negative control. .. The cleavage and control reactions were incubated at 37 °C for 3 h. Bsm DNA polymerase, Buffer R, and Nb.Bpu10I nicking endonuclease were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). .. Exponential isothermal amplification Two reaction mixtures were prepared separately on ice.

    Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
    Article Snippet: RNA sequences were analyzed by 8 M–urea 15% polyacrylamide gel electrophoresis (PAGE) and stained with SYBR green II for 20 min. Gels were visualized with a Molecular Imager FX Pro (Bio-Rad Laboratories). .. Chemical cross-linking analysis The purified ARMAN-2 tRNA endonuclease (∼500 ng) was incubated in PBS buffer containing 50 mM NaCl with various concentrations (0–5 mM) of BS3 (Bis[sulfosuccinimidyl] suberate) (Thermo Scientific, Rockford, IL, USA) at room temperature for 30 min. .. Reactions were stopped by adding 1 M Tris–HCl (pH 8.0) and analyzed by 10–20% PAGE containing SDS.

    Isolation:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

    Clone Assay:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

    Article Title: TILLING to detect induced mutations in soybean
    Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Genomic DNA restriction endonuclease digestion followed by high-throughput TILLING The gmrhg4 PCR product was amplified from the Forrest background and cloned using the pCR 4-TOPO TA kit (Invitrogen, Carlsbad, CA). .. Both strands of several clones were sequenced to generate consensus sequences for gmrhg4 and the homeolog/paralog.

    Sequencing:

    Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)
    Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the restriction endonuclease (RE) Mbo I, according to the manufacturers’ instructions (Invitrogen Life Technologies), resulting in a smear with DNA fragments ranging between 3 kb and 100 bp. .. The restriction products were later cloned using routine procedures (FERMENTAS Life Science, Invitrogen Life Technologies).

    Transfection:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    Construct:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    Expressing:

    Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
    Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Cas9 endonuclease together with ecotropic packaging plasmids, using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. .. Media containing viruses were collected 48 h after transfection and then utilized to infect HeLa and HT29 cells.

    High Throughput Screening Assay:

    Article Title: TILLING to detect induced mutations in soybean
    Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Genomic DNA restriction endonuclease digestion followed by high-throughput TILLING The gmrhg4 PCR product was amplified from the Forrest background and cloned using the pCR 4-TOPO TA kit (Invitrogen, Carlsbad, CA). .. Both strands of several clones were sequenced to generate consensus sequences for gmrhg4 and the homeolog/paralog.

    Amplification:

    Article Title: TILLING to detect induced mutations in soybean
    Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Genomic DNA restriction endonuclease digestion followed by high-throughput TILLING The gmrhg4 PCR product was amplified from the Forrest background and cloned using the pCR 4-TOPO TA kit (Invitrogen, Carlsbad, CA). .. Both strands of several clones were sequenced to generate consensus sequences for gmrhg4 and the homeolog/paralog.

    Article Title: Entamoeba moshkovskii Infections in Children in Bangladesh
    Article Snippet: In the nested PCR, 1.0 µL of first PCR product was used as the template DNA and the annealing temperature was raised to 62°C, leaving the other parameters of the amplification cycles unchanged. .. E. moshkovskii –specific nested SSU-rDNA gene amplification products were digested with restriction endonuclease Xho I for 1 h at 37°C according to the manufacturer’s instructions (Invitrogen Corp., Carlsbad, CA) to verify species identity. .. All PCR products were separated in 1.8% NuSieve 3:1 agarose gels (Flowgen, Lichfield, England) in 1x Tris-borate-EDTA buffer and visualized after staining with ethidium bromide (0.2 µg mL-1 ; Sigma-Aldrich Co. Ltd, Poole, England).

    Generated:

    Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes
    Article Snippet: Analyses of the melting curves generated by the two primers sets showed a shift in F2R2 primers when binding mutant cDNA, suggesting no wild type transcripts were detected ( ). .. In addition, cDNA generated from tmem88a mutants is not digested by ScaI endonuclease compared with wild type controls, providing further evidence that wild type transcripts are not present in tmem88a mutant embryos ( ). .. Reduced expression of some key cardiac and haematopoietic factors is observed in tmem88a mutant embryos Tmem88a knockdown has previously been shown to reduce expression of haematopoietic genes [ , ].

    Mutagenesis:

    Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes
    Article Snippet: Analyses of the melting curves generated by the two primers sets showed a shift in F2R2 primers when binding mutant cDNA, suggesting no wild type transcripts were detected ( ). .. In addition, cDNA generated from tmem88a mutants is not digested by ScaI endonuclease compared with wild type controls, providing further evidence that wild type transcripts are not present in tmem88a mutant embryos ( ). .. Reduced expression of some key cardiac and haematopoietic factors is observed in tmem88a mutant embryos Tmem88a knockdown has previously been shown to reduce expression of haematopoietic genes [ , ].

    Purification:

    Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
    Article Snippet: RNA sequences were analyzed by 8 M–urea 15% polyacrylamide gel electrophoresis (PAGE) and stained with SYBR green II for 20 min. Gels were visualized with a Molecular Imager FX Pro (Bio-Rad Laboratories). .. Chemical cross-linking analysis The purified ARMAN-2 tRNA endonuclease (∼500 ng) was incubated in PBS buffer containing 50 mM NaCl with various concentrations (0–5 mM) of BS3 (Bis[sulfosuccinimidyl] suberate) (Thermo Scientific, Rockford, IL, USA) at room temperature for 30 min. .. Reactions were stopped by adding 1 M Tris–HCl (pH 8.0) and analyzed by 10–20% PAGE containing SDS.

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    Thermo Fisher xbai restriction endonucleases
    Structure of pBud-h I L-7 expression vector. The IL-7 gene was inserted into the <t>SalI/XbaI</t> site of pBudCE4.1
    Xbai Restriction Endonucleases, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai restriction endonucleases/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai restriction endonucleases - by Bioz Stars, 2021-03
    94/100 stars
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    99
    Thermo Fisher restriction endonuclease xbai
    Structure of pBud-h I L-7 expression vector. The IL-7 gene was inserted into the <t>SalI/XbaI</t> site of pBudCE4.1
    Restriction Endonuclease Xbai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease xbai/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease xbai - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    Structure of pBud-h I L-7 expression vector. The IL-7 gene was inserted into the SalI/XbaI site of pBudCE4.1

    Journal: Reports of Biochemistry & Molecular Biology

    Article Title: Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells

    doi:

    Figure Lengend Snippet: Structure of pBud-h I L-7 expression vector. The IL-7 gene was inserted into the SalI/XbaI site of pBudCE4.1

    Article Snippet: After digestion with SalI and XbaI restriction endonucleases (Thermo Fisher Scientific, Vilnius, Lithuania), the gelpurified DNA fragment (Silica Bead DNA Gel Extraction Kit, Invitrogen, CA, USA) was inserted into the SalI/XbaI site of the pBudCE4.1 expression vector (Invitrogen) downstream of the CMV promoter to create pBud-h IL -7.

    Techniques: Expressing, Plasmid Preparation

    Agarose gel (1%) electrophoresis of restriction digestion of pBud-h IL-7 . lane M: DNA markers (GeneRuler 1 kb DNA Ladder,Thermo Fisher Scientific).Lane 1: pBud-hIL-7 cut with SalI and XbaI restriction endonucleases shows a 534 bp band expected for h IL-7 . Lane 2: uncut pBud-hIL-7 plasmid.

    Journal: Reports of Biochemistry & Molecular Biology

    Article Title: Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells

    doi:

    Figure Lengend Snippet: Agarose gel (1%) electrophoresis of restriction digestion of pBud-h IL-7 . lane M: DNA markers (GeneRuler 1 kb DNA Ladder,Thermo Fisher Scientific).Lane 1: pBud-hIL-7 cut with SalI and XbaI restriction endonucleases shows a 534 bp band expected for h IL-7 . Lane 2: uncut pBud-hIL-7 plasmid.

    Article Snippet: After digestion with SalI and XbaI restriction endonucleases (Thermo Fisher Scientific, Vilnius, Lithuania), the gelpurified DNA fragment (Silica Bead DNA Gel Extraction Kit, Invitrogen, CA, USA) was inserted into the SalI/XbaI site of the pBudCE4.1 expression vector (Invitrogen) downstream of the CMV promoter to create pBud-h IL -7.

    Techniques: Agarose Gel Electrophoresis, Electrophoresis, Plasmid Preparation