xbai restriction endonuclease (Thermo Fisher)
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Name:
Endonuclease V
Description:
Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
Catalog Number:
en0141
Price:
None
Category:
Proteins Enzymes Peptides
Applications:
Genotyping & Genomic Profiling|Rare & Low-Frequency Mutation Analysis|Gene Expression Analysis & Genotyping
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Structured Review
Thermo Fisher
xbai restriction endonuclease
Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
https://www.bioz.com/result/xbai restriction endonuclease/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Thermo Scientific Endonuclease V T maritima Endo V is a 3 endonuclease involved in DNA repair which initiates removal of deaminated bases from damaged DNA including uracil hypoxanthine and xanthine Endonuclease V is also active toward abasic sites and urea sites base pair mismatches flap and pseudo Y structures and small insertions deletions in DNA molecules The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3 to a lesion Highlights• Optimal activity at temperatures of 65 to 70°CApplications• High throughput methods for mutation research• Studies in mutagenesis and DNA repair• Mismatch cleavage• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license When the enzyme is in excess the primary nicked products experience a second nicking event on the complementary strand leading to a double stranded break At low concentrations however Endonuclease V first nicks a DNA strand at the lesions located closer to the 5 end of DNA molecule Single stranded DNA is cleaved with much lower efficiency Mg2 or Mn2 ions are required for enzyme activity
https://www.bioz.com/result/xbai restriction endonuclease/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
xbai restriction endonuclease - by Bioz Stars,
2021-03
99/100 stars
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Related Articles
Polymerase Chain Reaction:Article Title: Diversity of Green-Like and Red-Like Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes (cbbL) in Differently Managed Agricultural Soils Article Snippet: Each 10-μl digestion reaction mixture consisted of 2 μl of purified plasmid, 1 μl of buffer O+ (Fermentas GmbH), and 2 U of EcoRI (Fermentas GmbH) and was incubated at 37°C for 2 h. Clones which harbored a correctly sized red-like cbbL insert were screened by restriction fragment length polymorphism (RFLP). .. Ten microliters of each PCR product was hydrolyzed with 2 U of the Article Title: TILLING to detect induced mutations in soybean Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Incubation:Article Title: Multiplex quantitative analysis of microRNA expression via exponential isothermal amplification and conformation-sensitive DNA separation Article Snippet: The first 10 uL of extension reaction was used as the cleavage reaction and was added with 5 U of Nb.Bpu10I nicking endonuclease and 10 uL of 1× Buffer R. The remaining 10 uL was added with 10 uL of 1× buffer R and used as the negative control. .. The cleavage and control reactions were incubated at 37 °C for 3 h. Bsm DNA polymerase, Buffer R, and Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity Article Snippet: RNA sequences were analyzed by 8 M–urea 15% polyacrylamide gel electrophoresis (PAGE) and stained with SYBR green II for 20 min. Gels were visualized with a Molecular Imager FX Pro (Bio-Rad Laboratories). .. Chemical cross-linking analysis The purified Isolation:Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae) Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the Clone Assay:Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae) Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the Article Title: TILLING to detect induced mutations in soybean Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Sequencing:Article Title: Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae) Article Snippet: Genomic DNA of different species was obtained from these fibroblast cell cultures using the JETQUICK DNA kit (Genomed). .. Isolation, Cloning, and Sequencing of PSUcentSat Sequence PSU genomic DNA was digested with the Transfection:Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Construct:Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and Expressing:Article Title: HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect Article Snippet: HMGB1 knockout cell line was generated as described . .. In detail, 293 T cells (1 × 106 cells per well; 6-well plate) were transfected with lentiviral construct expressing two gRNAs (gRNA1: 5′ GGAAGAGTCGACTCGCTT 3′, gRNA2: 5′ GTGATGTTGCGAAGAAAC 3′) and High Throughput Screening Assay:Article Title: TILLING to detect induced mutations in soybean Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Amplification:Article Title: TILLING to detect induced mutations in soybean Article Snippet: LI-COR 4200 or 4300 (Lincoln, NE) gel images were analyzed using GelBuddy [ ]. .. Article Title: Entamoeba moshkovskii Infections in Children in Bangladesh Article Snippet: In the nested PCR, 1.0 µL of first PCR product was used as the template DNA and the annealing temperature was raised to 62°C, leaving the other parameters of the amplification cycles unchanged. .. E. moshkovskii –specific nested SSU-rDNA gene amplification products were digested with Generated:Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes Article Snippet: Analyses of the melting curves generated by the two primers sets showed a shift in F2R2 primers when binding mutant cDNA, suggesting no wild type transcripts were detected ( ). .. In addition, cDNA generated from tmem88a mutants is not digested by Mutagenesis:Article Title: Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes Article Snippet: Analyses of the melting curves generated by the two primers sets showed a shift in F2R2 primers when binding mutant cDNA, suggesting no wild type transcripts were detected ( ). .. In addition, cDNA generated from tmem88a mutants is not digested by Purification:Article Title: A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity Article Snippet: RNA sequences were analyzed by 8 M–urea 15% polyacrylamide gel electrophoresis (PAGE) and stained with SYBR green II for 20 min. Gels were visualized with a Molecular Imager FX Pro (Bio-Rad Laboratories). .. Chemical cross-linking analysis The purified |
