xbai endonuclease  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai endonuclease
    Xbai Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai endonuclease/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai endonuclease - by Bioz Stars, 2020-04
    86/100 stars

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    Incubation:

    Article Title: Epidemiology of Klebsiella oxytoca-Associated Diarrhea Detected by Simmons Citrate Agar Supplemented with Inositol, Tryptophan, and Bile Salts
    Article Snippet: .. Subsequently, a small portion of the plug (2 by 5 mm) was incubated with 50 U XbaI endonuclease (New England BioLabs, Beverly, MA) at 37°C with gentle shaking for 3 h. DNA fragments were resolved with 1% pulsed-field gel electrophoresis (PFGE) agarose in 250 ml 0.5× TBE buffer using a CHEF Mapper instrument (Bio-Rad). ..

    End-sequence Profiling:

    Article Title: Epidemiology of Klebsiella oxytoca-Associated Diarrhea Detected by Simmons Citrate Agar Supplemented with Inositol, Tryptophan, and Bile Salts
    Article Snippet: Plugs were then washed with ESP buffer (0.5 M EDTA [pH 9.0], 1% sodium lauryl sarcosine, 0.1 mg/ml proteinase K) at 55°C for 1 h twice. .. Subsequently, a small portion of the plug (2 by 5 mm) was incubated with 50 U XbaI endonuclease (New England BioLabs, Beverly, MA) at 37°C with gentle shaking for 3 h. DNA fragments were resolved with 1% pulsed-field gel electrophoresis (PFGE) agarose in 250 ml 0.5× TBE buffer using a CHEF Mapper instrument (Bio-Rad).

    Pulsed-Field Gel:

    Article Title: Epidemiology of Klebsiella oxytoca-Associated Diarrhea Detected by Simmons Citrate Agar Supplemented with Inositol, Tryptophan, and Bile Salts
    Article Snippet: .. Subsequently, a small portion of the plug (2 by 5 mm) was incubated with 50 U XbaI endonuclease (New England BioLabs, Beverly, MA) at 37°C with gentle shaking for 3 h. DNA fragments were resolved with 1% pulsed-field gel electrophoresis (PFGE) agarose in 250 ml 0.5× TBE buffer using a CHEF Mapper instrument (Bio-Rad). ..

    Software:

    Article Title: Epidemiology of Klebsiella oxytoca-Associated Diarrhea Detected by Simmons Citrate Agar Supplemented with Inositol, Tryptophan, and Bile Salts
    Article Snippet: Subsequently, a small portion of the plug (2 by 5 mm) was incubated with 50 U XbaI endonuclease (New England BioLabs, Beverly, MA) at 37°C with gentle shaking for 3 h. DNA fragments were resolved with 1% pulsed-field gel electrophoresis (PFGE) agarose in 250 ml 0.5× TBE buffer using a CHEF Mapper instrument (Bio-Rad). .. Gel patterns were analyzed with the BioNumerics software package (version 6.0; Applied-Maths, Belgium).

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    New England Biolabs restriction endonucleases xbai
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Restriction Endonucleases Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases xbai/product/New England Biolabs
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
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    New England Biolabs xbai endonuclease
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Xbai Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai endonuclease/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai endonuclease - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    90
    New England Biolabs restriction endonuclease digestion
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Restriction Endonuclease Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease digestion/product/New England Biolabs
    Average 90 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease digestion - by Bioz Stars, 2020-04
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    (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Journal: Applied and Environmental Microbiology

    Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain

    doi: 10.1128/AEM.01041-13

    Figure Lengend Snippet: (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Article Snippet: Pulsed-field gel electrophoresis (PFGE) was performed using the restriction endonucleases XbaI and AvrII (New England BioLabs) by following the CDC PulseNet protocol ( ).

    Techniques:

    ( A ) The 482 bp APC gene fragment ( APC cDNA bp 8422–8903) released by digestion of human genomic DNA with endonucleases Hae III and Xba I. The positions of the two biotinylated 30mer probes (BP1 and BP2) for enriching this fragment from genomic DNA are indicated by open bars. The positions of the AT→GC transition at APC cDNA bp 8652 carried by the internal standard and the recognition sites of endonucleases Sau 3AI and Acc I used to liberate the 271 bp APC gene fragment ( APC cDNA bp 8434–8704) are indicated by arrows. ( B ) The melting profile of the 271 bp APC gene fragment was calculated according to the melting algorithm of Lerman and Silverstein (23). The positions of the primers (AP1 and AP4H) used in high fidelity PCR are indicated by filled bars.

    Journal: Nucleic Acids Research

    Article Title: A sensitive scanning technology for low frequency nuclear point mutations in human genomic DNA

    doi:

    Figure Lengend Snippet: ( A ) The 482 bp APC gene fragment ( APC cDNA bp 8422–8903) released by digestion of human genomic DNA with endonucleases Hae III and Xba I. The positions of the two biotinylated 30mer probes (BP1 and BP2) for enriching this fragment from genomic DNA are indicated by open bars. The positions of the AT→GC transition at APC cDNA bp 8652 carried by the internal standard and the recognition sites of endonucleases Sau 3AI and Acc I used to liberate the 271 bp APC gene fragment ( APC cDNA bp 8434–8704) are indicated by arrows. ( B ) The melting profile of the 271 bp APC gene fragment was calculated according to the melting algorithm of Lerman and Silverstein (23). The positions of the primers (AP1 and AP4H) used in high fidelity PCR are indicated by filled bars.

    Article Snippet: The genomic DNA was digested with two ‘inexpensive’ endonucleases, Hae III and Xba I (New England Biolabs, Beverly, MA), at 1 U enzyme/µg DNA and 2–3 mg DNA/ml overnight to liberate the target sequence embedded in a 482 bp fragment representing the APC cDNA bp 8422–8903.

    Techniques: Polymerase Chain Reaction