xba i  (Thermo Fisher)


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    Name:
    XbaI
    Description:
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0683
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher xba i
    Combined numerical analysis of PFGE results obtained after <t>Xba</t> I and <t>Dra</t> I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/xba i/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xba i - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe"

    Article Title: A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe

    Journal: Journal of Clinical Microbiology

    doi:

    Combined numerical analysis of PFGE results obtained after Xba I and Dra I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.
    Figure Legend Snippet: Combined numerical analysis of PFGE results obtained after Xba I and Dra I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.

    Techniques Used:

    RAPD (A to D) and PFGE (E to H) profiles of M. simiae isolates. Representative RAPD patterns with the primers 4 (A) and 10 (C) for one isolate per patient and the results obtained with serial isolates from selected patients with primer 4 (B and D) are shown. Panels E and F show representative PFGE patterns obtained with Xba I- and Dra I-digested DNAs for patients with polyclonal infections. All of the 22 PFGE profiles obtained are illustrated in dendrograms shown in panels G and H. Samples for RAPD experiments were run in duplicate. T, template DNA control; M, molecular weight marker; A to I, individual patient isolates (A1, A2, etc., represent serial isolates from the same patient). The scale in panels G and H shows the Dice index.
    Figure Legend Snippet: RAPD (A to D) and PFGE (E to H) profiles of M. simiae isolates. Representative RAPD patterns with the primers 4 (A) and 10 (C) for one isolate per patient and the results obtained with serial isolates from selected patients with primer 4 (B and D) are shown. Panels E and F show representative PFGE patterns obtained with Xba I- and Dra I-digested DNAs for patients with polyclonal infections. All of the 22 PFGE profiles obtained are illustrated in dendrograms shown in panels G and H. Samples for RAPD experiments were run in duplicate. T, template DNA control; M, molecular weight marker; A to I, individual patient isolates (A1, A2, etc., represent serial isolates from the same patient). The scale in panels G and H shows the Dice index.

    Techniques Used: Molecular Weight, Marker

    2) Product Images from "Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase"

    Article Title: Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00191.2009

    The 576-bp PcCdc42 probe hybridizes to Pneumocystis genomic DNA. Freshly isolated Pc ( lanes 1–3 ) and rat ( lanes 5–7 ) genomic DNA were digested with the restriction endonucleases Bam HI ( lanes 1 and 5 ), Xho I ( lanes 2 and 6 ), and Xba I ( lanes 3 and 7 ). The digestion products were separated by electrophoresis and transferred to nitrocellulose. The 576-bp PcCdc42 amplicon was labeled and hybridized to the membrane showing specific interaction with the Pc digestions. No hybridization was noted in the rat genomic DNA lane.
    Figure Legend Snippet: The 576-bp PcCdc42 probe hybridizes to Pneumocystis genomic DNA. Freshly isolated Pc ( lanes 1–3 ) and rat ( lanes 5–7 ) genomic DNA were digested with the restriction endonucleases Bam HI ( lanes 1 and 5 ), Xho I ( lanes 2 and 6 ), and Xba I ( lanes 3 and 7 ). The digestion products were separated by electrophoresis and transferred to nitrocellulose. The 576-bp PcCdc42 amplicon was labeled and hybridized to the membrane showing specific interaction with the Pc digestions. No hybridization was noted in the rat genomic DNA lane.

    Techniques Used: Isolation, Electrophoresis, Amplification, Labeling, Hybridization

    3) Product Images from "The bandit, a New DNA Transposon from a Hookworm--Possible Horizontal Genetic Transfer between Host and Parasite"

    Article Title: The bandit, a New DNA Transposon from a Hookworm--Possible Horizontal Genetic Transfer between Host and Parasite

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0000035

    Southern hybridization analysis of Ancylostoma caninum genomic DNA to a probe specific for the bandit transposon. The genomic DNAs were cleaved with endonucleases Xba I (lane 1) and Xho I (lane 2). Molecular size standards in kilobase pairs (kb) are shown at the left.
    Figure Legend Snippet: Southern hybridization analysis of Ancylostoma caninum genomic DNA to a probe specific for the bandit transposon. The genomic DNAs were cleaved with endonucleases Xba I (lane 1) and Xho I (lane 2). Molecular size standards in kilobase pairs (kb) are shown at the left.

    Techniques Used: Hybridization

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: The bandit, a New DNA Transposon from a Hookworm--Possible Horizontal Genetic Transfer between Host and Parasite
    Article Snippet: Construction and screening of hookworm genomic DNA libraries; bioinformatics Size selected plasmid libraries of gDNA from adult A. caninum were constructed as described . .. Briefly, gDNA was digested with the endonuclease Hin d III and Xba I (Fermentas, Sweden) and size separated through 0.8% agarose gel. .. Fragments ranging in size from 2–7 kilobase pairs (kb) were excised, eluted from the gel, and ligated into plasmid pBluescript SK (+/−) (Stratagene).

    Article Title: A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe
    Article Snippet: Bacteria were grown in 5 ml of Middlebrook 7H9 complete broth. .. Plugs were prepared as previously described , and bacterial DNA was digested with 30 U of Dra I or 60 U of Xba I (Gibco-BRL Life Technologies) at 37°C for 2 h. After digestion, the plugs were loaded into a 1% (wt/vol) agarose gel (Gibco BRL). .. Large restriction fragments were separated using the contour-clamped homogeneous electric field DRIII pulsed-field gel electrophoresis (PFGE) apparatus (Bio-Rad, Richmond, Calif.) for 24 h at 14°C and 6 V/cm with a switch time of 1 to 40 s for Dra I and for 20 h at 14°C and 6 V/cm with a switch time of 1 to 30 s for Xba I.

    Labeling:

    Article Title: Developmental Specificity of the Interaction between the Locus Control Region and Embryonic or Fetal Globin Genes in Transgenic Mice with an HS3 Core Deletion
    Article Snippet: A 753-bp Hin dIII fragment containing sequences 3′ of the A γ-globin gene (GenBank coordinates 41382 to 42135) was cloned into pW126, a pBluescript (Stratagene) plasmid containing a 544-bp Bam HI Thy1.1 cDNA, to produce pThy1.1/3′ A γ(753). .. Digestion with Xba I and Xho I released a 1.3-kb fragment that was labeled with a Decaprime II random labeling kit (Ambion). .. As an internal control during Southern blot hybridization, we digested pThy1.1/3′ A γ(753) with Pst I and Sca I to release a 2.6-kb A γ fragment and a 1.6-kb Thy1.1 fragment.

    Amplification:

    Article Title: A decamer duplication in the 3? region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred
    Article Snippet: In both cases, the formic acid pretreatment was omitted, and anti-rabbit (Dako 1:200) or anti-mouse (Dako, 1:200) secondary antibodies were used, as appropriate. .. Amplification of genomic DNA samples isolated from peripheral blood leukocytes of living FDD family members, as well as brain tissue obtained postmortem, was performed as described in ref. , by using oligonucleotide primer pairs F: 5′-CGT GAA GCC AGC AAT TGT TTC GCA-3′ and R: 5′-AGCCCTGTTTGCTACTTACATG-3′ (product = 191 bp) by PCR by using 250 μmol dNTPs/2.5 mM MgCl2 /50 pmol oligonucleotides, in 100 μl, cycled for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. Restriction enzyme analysis of the amplified PCR products was performed with Xba I (GIBCO/BRL) according to the manufacturer's protocol, and the resulting products were resolved by nondenaturing 5% PAGE. .. PCR amplification by using oligonucleotide primer pairs F and R2: 5′-ACC AAA TTG TAA AGG GTG GG -3′ was done by using 250 μmol dNTPs, 2.5 mM MgCl2 , 50 pmol oligonucleotides, in 100 μl, for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. PCR products were cloned into pCR 2.1 vector (Invitrogen) and sequenced by automated cycle sequencing in both directions.

    Article Title: Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase
    Article Snippet: To further verify the authenticity of the final full-length 576-bp reading frame, this PCR product was radiolabeled and hybridized to Pc chromosomes separated by CHEF blot as previously described ( ). .. In addition, the 576-bp amplicon was also hybridized to Pc genomic DNA digested with the restriction enzymes Bam HI, Xho I, and Xba I (Invitrogen). .. In parallel reactions, rat genomic DNA (Bioline, Randolph, MA) was digested with the same restriction enzymes and hybridized in a similar fashion.

    Isolation:

    Article Title: A decamer duplication in the 3? region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred
    Article Snippet: In both cases, the formic acid pretreatment was omitted, and anti-rabbit (Dako 1:200) or anti-mouse (Dako, 1:200) secondary antibodies were used, as appropriate. .. Amplification of genomic DNA samples isolated from peripheral blood leukocytes of living FDD family members, as well as brain tissue obtained postmortem, was performed as described in ref. , by using oligonucleotide primer pairs F: 5′-CGT GAA GCC AGC AAT TGT TTC GCA-3′ and R: 5′-AGCCCTGTTTGCTACTTACATG-3′ (product = 191 bp) by PCR by using 250 μmol dNTPs/2.5 mM MgCl2 /50 pmol oligonucleotides, in 100 μl, cycled for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. Restriction enzyme analysis of the amplified PCR products was performed with Xba I (GIBCO/BRL) according to the manufacturer's protocol, and the resulting products were resolved by nondenaturing 5% PAGE. .. PCR amplification by using oligonucleotide primer pairs F and R2: 5′-ACC AAA TTG TAA AGG GTG GG -3′ was done by using 250 μmol dNTPs, 2.5 mM MgCl2 , 50 pmol oligonucleotides, in 100 μl, for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. PCR products were cloned into pCR 2.1 vector (Invitrogen) and sequenced by automated cycle sequencing in both directions.

    Polymerase Chain Reaction:

    Article Title: A decamer duplication in the 3? region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred
    Article Snippet: In both cases, the formic acid pretreatment was omitted, and anti-rabbit (Dako 1:200) or anti-mouse (Dako, 1:200) secondary antibodies were used, as appropriate. .. Amplification of genomic DNA samples isolated from peripheral blood leukocytes of living FDD family members, as well as brain tissue obtained postmortem, was performed as described in ref. , by using oligonucleotide primer pairs F: 5′-CGT GAA GCC AGC AAT TGT TTC GCA-3′ and R: 5′-AGCCCTGTTTGCTACTTACATG-3′ (product = 191 bp) by PCR by using 250 μmol dNTPs/2.5 mM MgCl2 /50 pmol oligonucleotides, in 100 μl, cycled for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. Restriction enzyme analysis of the amplified PCR products was performed with Xba I (GIBCO/BRL) according to the manufacturer's protocol, and the resulting products were resolved by nondenaturing 5% PAGE. .. PCR amplification by using oligonucleotide primer pairs F and R2: 5′-ACC AAA TTG TAA AGG GTG GG -3′ was done by using 250 μmol dNTPs, 2.5 mM MgCl2 , 50 pmol oligonucleotides, in 100 μl, for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. PCR products were cloned into pCR 2.1 vector (Invitrogen) and sequenced by automated cycle sequencing in both directions.

    Article Title: Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
    Article Snippet: For the T7EI assay, the PCR product was cleaned up and digested with T7EI (New England Biolabs following the manufacturer’s instructions. .. For the RLFP assay, PCR products were digested with the restriction enzyme XbaI (Thermo Scientific). .. Cleaved DNA fragments were separated on 2 % agarose gels and the DNA concentration of each band was quantified using the ImageJ software.

    Polyacrylamide Gel Electrophoresis:

    Article Title: A decamer duplication in the 3? region of the BRI gene originates an amyloid peptide that is associated with dementia in a Danish kindred
    Article Snippet: In both cases, the formic acid pretreatment was omitted, and anti-rabbit (Dako 1:200) or anti-mouse (Dako, 1:200) secondary antibodies were used, as appropriate. .. Amplification of genomic DNA samples isolated from peripheral blood leukocytes of living FDD family members, as well as brain tissue obtained postmortem, was performed as described in ref. , by using oligonucleotide primer pairs F: 5′-CGT GAA GCC AGC AAT TGT TTC GCA-3′ and R: 5′-AGCCCTGTTTGCTACTTACATG-3′ (product = 191 bp) by PCR by using 250 μmol dNTPs/2.5 mM MgCl2 /50 pmol oligonucleotides, in 100 μl, cycled for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. Restriction enzyme analysis of the amplified PCR products was performed with Xba I (GIBCO/BRL) according to the manufacturer's protocol, and the resulting products were resolved by nondenaturing 5% PAGE. .. PCR amplification by using oligonucleotide primer pairs F and R2: 5′-ACC AAA TTG TAA AGG GTG GG -3′ was done by using 250 μmol dNTPs, 2.5 mM MgCl2 , 50 pmol oligonucleotides, in 100 μl, for 30 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 30 s. PCR products were cloned into pCR 2.1 vector (Invitrogen) and sequenced by automated cycle sequencing in both directions.

    Construct:

    Article Title: Cloning and Expression of Two Related Connexins from the Perch Retina Define a Distinct Subgroup of the Connexin Family
    Article Snippet: Cx34.7 (a 1871 bp fragment) and Cx35 (a 1320 bp fragment) were excised by Eco RI/ Xba I (Boehringer Mannheim) digestion of pBluescript clones, blunted with the Klenow fragment of DNA polymerase I (Pharmacia-Biotech, Orsay, France), and subcloned into the Bgl II site of the expression vector pSP64T ( ). .. Constructs were linearized with Xba I, and capped cRNAs were produced in vitro with SP6 RNA polymerase, using the mMessage mMachine Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. .. The purity and yield of transcribed cRNAs were determined by visualizing their integrity on agarose gels stained with ethidium bromide and by absorbance measurements at 260 and 280 nm.

    Produced:

    Article Title: Cloning and Expression of Two Related Connexins from the Perch Retina Define a Distinct Subgroup of the Connexin Family
    Article Snippet: Cx34.7 (a 1871 bp fragment) and Cx35 (a 1320 bp fragment) were excised by Eco RI/ Xba I (Boehringer Mannheim) digestion of pBluescript clones, blunted with the Klenow fragment of DNA polymerase I (Pharmacia-Biotech, Orsay, France), and subcloned into the Bgl II site of the expression vector pSP64T ( ). .. Constructs were linearized with Xba I, and capped cRNAs were produced in vitro with SP6 RNA polymerase, using the mMessage mMachine Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. .. The purity and yield of transcribed cRNAs were determined by visualizing their integrity on agarose gels stained with ethidium bromide and by absorbance measurements at 260 and 280 nm.

    In Vitro:

    Article Title: Cloning and Expression of Two Related Connexins from the Perch Retina Define a Distinct Subgroup of the Connexin Family
    Article Snippet: Cx34.7 (a 1871 bp fragment) and Cx35 (a 1320 bp fragment) were excised by Eco RI/ Xba I (Boehringer Mannheim) digestion of pBluescript clones, blunted with the Klenow fragment of DNA polymerase I (Pharmacia-Biotech, Orsay, France), and subcloned into the Bgl II site of the expression vector pSP64T ( ). .. Constructs were linearized with Xba I, and capped cRNAs were produced in vitro with SP6 RNA polymerase, using the mMessage mMachine Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. .. The purity and yield of transcribed cRNAs were determined by visualizing their integrity on agarose gels stained with ethidium bromide and by absorbance measurements at 260 and 280 nm.

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    Thermo Fisher xba i
    Combined numerical analysis of PFGE results obtained after <t>Xba</t> I and <t>Dra</t> I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.
    Xba I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xba i/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xba i - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Combined numerical analysis of PFGE results obtained after Xba I and Dra I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.

    Journal: Journal of Clinical Microbiology

    Article Title: A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe

    doi:

    Figure Lengend Snippet: Combined numerical analysis of PFGE results obtained after Xba I and Dra I digestions using the unweighted pair group method using arithmetic averages. A to I, individual patient isolates (A1, A2, etc., represent serial isolates from a same patient). The scale shows the Dice index.

    Article Snippet: Plugs were prepared as previously described , and bacterial DNA was digested with 30 U of Dra I or 60 U of Xba I (Gibco-BRL Life Technologies) at 37°C for 2 h. After digestion, the plugs were loaded into a 1% (wt/vol) agarose gel (Gibco BRL).

    Techniques:

    RAPD (A to D) and PFGE (E to H) profiles of M. simiae isolates. Representative RAPD patterns with the primers 4 (A) and 10 (C) for one isolate per patient and the results obtained with serial isolates from selected patients with primer 4 (B and D) are shown. Panels E and F show representative PFGE patterns obtained with Xba I- and Dra I-digested DNAs for patients with polyclonal infections. All of the 22 PFGE profiles obtained are illustrated in dendrograms shown in panels G and H. Samples for RAPD experiments were run in duplicate. T, template DNA control; M, molecular weight marker; A to I, individual patient isolates (A1, A2, etc., represent serial isolates from the same patient). The scale in panels G and H shows the Dice index.

    Journal: Journal of Clinical Microbiology

    Article Title: A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe

    doi:

    Figure Lengend Snippet: RAPD (A to D) and PFGE (E to H) profiles of M. simiae isolates. Representative RAPD patterns with the primers 4 (A) and 10 (C) for one isolate per patient and the results obtained with serial isolates from selected patients with primer 4 (B and D) are shown. Panels E and F show representative PFGE patterns obtained with Xba I- and Dra I-digested DNAs for patients with polyclonal infections. All of the 22 PFGE profiles obtained are illustrated in dendrograms shown in panels G and H. Samples for RAPD experiments were run in duplicate. T, template DNA control; M, molecular weight marker; A to I, individual patient isolates (A1, A2, etc., represent serial isolates from the same patient). The scale in panels G and H shows the Dice index.

    Article Snippet: Plugs were prepared as previously described , and bacterial DNA was digested with 30 U of Dra I or 60 U of Xba I (Gibco-BRL Life Technologies) at 37°C for 2 h. After digestion, the plugs were loaded into a 1% (wt/vol) agarose gel (Gibco BRL).

    Techniques: Molecular Weight, Marker

    The 576-bp PcCdc42 probe hybridizes to Pneumocystis genomic DNA. Freshly isolated Pc ( lanes 1–3 ) and rat ( lanes 5–7 ) genomic DNA were digested with the restriction endonucleases Bam HI ( lanes 1 and 5 ), Xho I ( lanes 2 and 6 ), and Xba I ( lanes 3 and 7 ). The digestion products were separated by electrophoresis and transferred to nitrocellulose. The 576-bp PcCdc42 amplicon was labeled and hybridized to the membrane showing specific interaction with the Pc digestions. No hybridization was noted in the rat genomic DNA lane.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase

    doi: 10.1152/ajplung.00191.2009

    Figure Lengend Snippet: The 576-bp PcCdc42 probe hybridizes to Pneumocystis genomic DNA. Freshly isolated Pc ( lanes 1–3 ) and rat ( lanes 5–7 ) genomic DNA were digested with the restriction endonucleases Bam HI ( lanes 1 and 5 ), Xho I ( lanes 2 and 6 ), and Xba I ( lanes 3 and 7 ). The digestion products were separated by electrophoresis and transferred to nitrocellulose. The 576-bp PcCdc42 amplicon was labeled and hybridized to the membrane showing specific interaction with the Pc digestions. No hybridization was noted in the rat genomic DNA lane.

    Article Snippet: In addition, the 576-bp amplicon was also hybridized to Pc genomic DNA digested with the restriction enzymes Bam HI, Xho I, and Xba I (Invitrogen).

    Techniques: Isolation, Electrophoresis, Amplification, Labeling, Hybridization

    Southern hybridization analysis of Ancylostoma caninum genomic DNA to a probe specific for the bandit transposon. The genomic DNAs were cleaved with endonucleases Xba I (lane 1) and Xho I (lane 2). Molecular size standards in kilobase pairs (kb) are shown at the left.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: The bandit, a New DNA Transposon from a Hookworm--Possible Horizontal Genetic Transfer between Host and Parasite

    doi: 10.1371/journal.pntd.0000035

    Figure Lengend Snippet: Southern hybridization analysis of Ancylostoma caninum genomic DNA to a probe specific for the bandit transposon. The genomic DNAs were cleaved with endonucleases Xba I (lane 1) and Xho I (lane 2). Molecular size standards in kilobase pairs (kb) are shown at the left.

    Article Snippet: Briefly, gDNA was digested with the endonuclease Hin d III and Xba I (Fermentas, Sweden) and size separated through 0.8% agarose gel.

    Techniques: Hybridization

    Detection of the 3′ β enhancer (3′β enh) in line D by Southern analysis. (A) Map of the location of the 260-bp Pst I fragment containing the 3′ β enhancer (indicated by the hatched box) relative to the β-globin gene (indicated by the black box). Restriction enzyme sites and GenBank coordinates are shown below the map. The fragment sizes in wild-type DNA indicated below the map are 4.9 kb for Bgl II, 3.7 kb for Eco RI, and 1.9 kb for Eco RI- Xba I. (B) Southern analysis of DNAs from the wild-type (WT) β-YAC line and line D digested with the restriction enzymes indicated above each lane as follows: B, Bgl II; E, Eco RI; and E/X, Eco RI- Xba I. The probe was a 771-bp Eco RI- Pst I fragment indicated in panel A as a solid bar. Migration positions of λ Hin dIII size markers are indicated to the right of the autoradiogram, and positions of molecular size markers (M) (in kilobases) are indicated to the left.

    Journal: Molecular and Cellular Biology

    Article Title: Developmental Specificity of the Interaction between the Locus Control Region and Embryonic or Fetal Globin Genes in Transgenic Mice with an HS3 Core Deletion

    doi:

    Figure Lengend Snippet: Detection of the 3′ β enhancer (3′β enh) in line D by Southern analysis. (A) Map of the location of the 260-bp Pst I fragment containing the 3′ β enhancer (indicated by the hatched box) relative to the β-globin gene (indicated by the black box). Restriction enzyme sites and GenBank coordinates are shown below the map. The fragment sizes in wild-type DNA indicated below the map are 4.9 kb for Bgl II, 3.7 kb for Eco RI, and 1.9 kb for Eco RI- Xba I. (B) Southern analysis of DNAs from the wild-type (WT) β-YAC line and line D digested with the restriction enzymes indicated above each lane as follows: B, Bgl II; E, Eco RI; and E/X, Eco RI- Xba I. The probe was a 771-bp Eco RI- Pst I fragment indicated in panel A as a solid bar. Migration positions of λ Hin dIII size markers are indicated to the right of the autoradiogram, and positions of molecular size markers (M) (in kilobases) are indicated to the left.

    Article Snippet: Digestion with Xba I and Xho I released a 1.3-kb fragment that was labeled with a Decaprime II random labeling kit (Ambion).

    Techniques: Migration