Journal: Microbial Cell Factories

Article Title: Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?

doi: 10.1186/1475-2859-7-3

Figure Lengend Snippet: HPLC analysis of β-carotene and xanthophylls produced by A) X. dendrorhous ATCC 96815 or B) Mucor circinelloides MS12, before and after transformation with the crtS gene. CR, cryptoxanthin; ZX, zeaxanthin; AX, astaxanthin; HDCO, 3-hydroxy-3',4'-didehydro-β-ψ-carotene-4-one. Note that expression of the crtS gene in M. circinelloides results in the production of β-carotene hydroxylated derivatives only, whereas transformants of X. dendrorhous with the same gene produce astaxanthin in addition to the hydroxylated derivatives.

Article Snippet: Complementation of the X. dendrorhous ATCC 96815 with the crtS gene has also been reported by Ojima et al. [ ].

Techniques: Produced, Transformation Assay, Expressing

Journal: Microbial Cell Factories

Article Title: Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?

doi: 10.1186/1475-2859-7-3

Figure Lengend Snippet: HPLC analysis of β-carotene and xanthophylls produced by A) X. dendrorhous ATCC 96815 or B) Mucor circinelloides MS12, before and after transformation with the crtS gene. CR, cryptoxanthin; ZX, zeaxanthin; AX, astaxanthin; HDCO, 3-hydroxy-3',4'-didehydro-β-ψ-carotene-4-one. Note that expression of the crtS gene in M. circinelloides results in the production of β-carotene hydroxylated derivatives only, whereas transformants of X. dendrorhous with the same gene produce astaxanthin in addition to the hydroxylated derivatives.

Article Snippet: In addition ATCC 96815 transformed with the crtS gene produced HDCO (3'-hydroxy-3',4'-didehydro-β,ψ-carotene-4-one) that is not formed in the untransformed ATCC 96815 strain.

Techniques: Produced, Transformation Assay, Expressing

Journal: Microbial Cell Factories

Article Title: Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?

doi: 10.1186/1475-2859-7-3

Figure Lengend Snippet: HPLC analysis of β-carotene and xanthophylls produced by A) X. dendrorhous ATCC 96815 or B) Mucor circinelloides MS12, before and after transformation with the crtS gene. CR, cryptoxanthin; ZX, zeaxanthin; AX, astaxanthin; HDCO, 3-hydroxy-3',4'-didehydro-β-ψ-carotene-4-one. Note that expression of the crtS gene in M. circinelloides results in the production of β-carotene hydroxylated derivatives only, whereas transformants of X. dendrorhous with the same gene produce astaxanthin in addition to the hydroxylated derivatives.

Article Snippet: Complementation of the strain ATCC 96815 with the crtS gene restored high levels of astaxanthin production, but also led to the accumulation of the intermediates cryptoxanthin (monohydroxylated derivative of β-carotene at C-3) and zeaxanthin (a 3,3'-dihydroxy-derivative of β-carotene).

Techniques: Produced, Transformation Assay, Expressing

Journal: International Journal of Nanomedicine

Article Title: Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes

doi: 10.2147/IJN.S152010

Figure Lengend Snippet: Physicochemical properties of AgNPs and AuNPs prepared by Phaffia rhodozyma cell-free extract. Transmission electron microscopic images of AgNPs and AuNPs ( A ), and size distribution of the nanoparticles by DLS measurements ( B ), UV-VIS spectra ( C ) and X-ray diffraction pattern ( D ). Abbreviations: AgNP, silver nanoparticle; AuNP, gold nanoparticle; DLS, dynamic light scattering; UV-VIS, ultraviolet-visible.

Article Snippet: P. rhodozyma American Type Culture Collection (ATCC) 24203 cells grown on 2× yeast extract peptone dextrose (YPD; Sigma-Aldrich Co., St Louis, MO, USA) (2% D-glucose, 2% peptone, 1% yeast extract and 2% agar) plates at 22°C for 5 days were collected by cell scraper.

Techniques: Transmission Assay

Journal: EFSA Journal

Article Title: Safety and efficacy of a feed additive consisting of astaxanthin‐rich Phaffia rhodozyma for salmon and trout (Igene Biotechnology, Inc.)

doi: 10.2903/j.efsa.2022.7161

Figure Lengend Snippet: Carotenoid content in astaxanthin‐rich Xanthophyllomyces dendrorhous (ATCC SD‐5340) from batches manufactured in 2019

Article Snippet: The Panel concluded that the use of Xanthophyllomyces dendrorhous ( Phaffia rhodozyma ) ATCC SD‐5340 in the production of the additive would not raise any safety concerns for the target species, the consumers of products from animals fed the additive, and the environment.

Techniques:

Journal: EFSA Journal

Article Title: Safety and efficacy of a feed additive consisting of astaxanthin‐rich Phaffia rhodozyma for salmon and trout (Igene Biotechnology, Inc.)

doi: 10.2903/j.efsa.2022.7161

Figure Lengend Snippet: Carotenoid content in astaxanthin‐rich Xanthophyllomyces dendrorhous (ATCC SD‐5340) from batches manufactured in 2019

Article Snippet: The additive under assessment is produced by fermentation using the yeast Xanthophyllomyces dendrorhous ATCC SD‐5340, which is considered the telemorph of Phaffia rhodozyma .

Techniques: