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Biotechnology Information wt tlr2 structure
Expression levels and signaling capacities of WT and R753Q <t>YFP-TLR2</t> species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,
Wt Tlr2 Structure, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *"

Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.375493

Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,
Figure Legend Snippet: Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,

Techniques Used: Expressing, Stable Transfection, Transfection

The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (
Figure Legend Snippet: The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (

Techniques Used: Expressing, Stable Transfection, Transfection

Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD
Figure Legend Snippet: Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD

Techniques Used: Mutagenesis

The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding
Figure Legend Snippet: The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding

Techniques Used: Transfection

The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells
Figure Legend Snippet: The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells

Techniques Used: Stable Transfection, Expressing, Transfection

R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,
Figure Legend Snippet: R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,

Techniques Used: Transfection

The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with
Figure Legend Snippet: The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with

Techniques Used: Expressing

R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected
Figure Legend Snippet: R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected

Techniques Used: Activation Assay, Luciferase, Derivative Assay, Laser Capture Microdissection, Transfection

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    Biotechnology Information wt tlr2 structure
    Expression levels and signaling capacities of WT and R753Q <t>YFP-TLR2</t> species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,
    Wt Tlr2 Structure, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt tlr2 structure/product/Biotechnology Information
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt tlr2 structure - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing, Stable Transfection, Transfection

    The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing, Stable Transfection, Transfection

    Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Mutagenesis

    The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Transfection

    The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Stable Transfection, Expressing, Transfection

    R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Transfection

    The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing

    R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Activation Assay, Luciferase, Derivative Assay, Laser Capture Microdissection, Transfection