wt ovation pico rna amplification system  (Nugen)

 
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    Nugen wt ovation pico rna amplification system
    Removal of 16s and <t>23s</t> rRNAs using MICROB Express ™ kit from Ambion . Total <t>RNA</t> samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.
    Wt Ovation Pico Rna Amplification System, supplied by Nugen, used in various techniques. Bioz Stars score: 92/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt ovation pico rna amplification system/product/Nugen
    Average 92 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    wt ovation pico rna amplification system - by Bioz Stars, 2021-01
    92/100 stars

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    Images

    1) Product Images from "Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system"

    Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-8-72

    Removal of 16s and 23s rRNAs using MICROB Express ™ kit from Ambion . Total RNA samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.
    Figure Legend Snippet: Removal of 16s and 23s rRNAs using MICROB Express ™ kit from Ambion . Total RNA samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.

    Techniques Used:

    RT-PCR of 20 gene candidates . Lane 1: low molecular weight DNA ladder from New England Biolabs. Size range: 25 bp to 766 bp. Lane 2-21: 20 gene candidates, VBISMa0080, VBISMa0492, VBISMa1337, VBISMb0078, VBISMb0839, VBISMc0095, VBISMc0802, VBISMc1000, VBISMc1221, VBISMc1492, VBISMc1793, VBISMc2171, VBISMc2174, VBISMc2596, VBISMc2940, VBISMc2955, VBISMc3188, VBISMc3282, VBISMc4046 and VBISMc4289, respectively. For majority RT-PCR reactions, each produced one corresponding PCR product (lane 3-5, 7, 9-12, 14-19 and 21). These PCR products were directly sequenced and their sequences matched to the corresponding gene candidates. Multiple PCR products were found in lane 2, 6, 8, 13 and 20. The bands with the correct PCR product sizes are labeled with *. These PCR products were used to do a second round of PCR to produce enough DNA for sequencing. The sequencing results confirmed that they matched to the corresponding gene candidates. The most abundant PCR product in lane 2 was sequenced and determined to be a part of 23s rRNA sequence. Lane 22-29: negative controls using the RNA sample that was not reverse transcribed and primer pairs of new genes to show no genomic DNA contamination. In each lane of 22 to 29, combined primer pairs of two or three genes were used. Lane 30: no template control. Primer pairs of cm0012a, cm012b and cm016a, cm016b were used.
    Figure Legend Snippet: RT-PCR of 20 gene candidates . Lane 1: low molecular weight DNA ladder from New England Biolabs. Size range: 25 bp to 766 bp. Lane 2-21: 20 gene candidates, VBISMa0080, VBISMa0492, VBISMa1337, VBISMb0078, VBISMb0839, VBISMc0095, VBISMc0802, VBISMc1000, VBISMc1221, VBISMc1492, VBISMc1793, VBISMc2171, VBISMc2174, VBISMc2596, VBISMc2940, VBISMc2955, VBISMc3188, VBISMc3282, VBISMc4046 and VBISMc4289, respectively. For majority RT-PCR reactions, each produced one corresponding PCR product (lane 3-5, 7, 9-12, 14-19 and 21). These PCR products were directly sequenced and their sequences matched to the corresponding gene candidates. Multiple PCR products were found in lane 2, 6, 8, 13 and 20. The bands with the correct PCR product sizes are labeled with *. These PCR products were used to do a second round of PCR to produce enough DNA for sequencing. The sequencing results confirmed that they matched to the corresponding gene candidates. The most abundant PCR product in lane 2 was sequenced and determined to be a part of 23s rRNA sequence. Lane 22-29: negative controls using the RNA sample that was not reverse transcribed and primer pairs of new genes to show no genomic DNA contamination. In each lane of 22 to 29, combined primer pairs of two or three genes were used. Lane 30: no template control. Primer pairs of cm0012a, cm012b and cm016a, cm016b were used.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Produced, Polymerase Chain Reaction, Labeling, Sequencing

    2) Product Images from "Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology"

    Article Title: Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0013564

    Flowchart of experimental methodology. Cell culture supernatants containing viral particles was collected from HIV infected cells. The RNA was extracted using the QIAamp Viral RNA Mini Kit and coverted into large quantities of single stranded DNA using the WT-Ovation Pico RNA Amplification System. The complementary strand for the ssDNA was then synthesized using the WT-Ovation Exon Module. The final step in the process involved using the Genomic DNA Sample Prep Kit to produce an Illumina library.
    Figure Legend Snippet: Flowchart of experimental methodology. Cell culture supernatants containing viral particles was collected from HIV infected cells. The RNA was extracted using the QIAamp Viral RNA Mini Kit and coverted into large quantities of single stranded DNA using the WT-Ovation Pico RNA Amplification System. The complementary strand for the ssDNA was then synthesized using the WT-Ovation Exon Module. The final step in the process involved using the Genomic DNA Sample Prep Kit to produce an Illumina library.

    Techniques Used: Cell Culture, Infection, Amplification, Synthesized, Sample Prep

    3) Product Images from "Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling"

    Article Title: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-10-246

    Reproducibility of expression data obtained using Nugen chemistry across amounts of input RNA . Pearson's correlation coefficients were computed and represented graphically for each pairwise comparison of assays. Lower correlations were observed for data obtained from smallest amounts of input RNA (50 pg and 100 pg).
    Figure Legend Snippet: Reproducibility of expression data obtained using Nugen chemistry across amounts of input RNA . Pearson's correlation coefficients were computed and represented graphically for each pairwise comparison of assays. Lower correlations were observed for data obtained from smallest amounts of input RNA (50 pg and 100 pg).

    Techniques Used: Expressing

    Quality of cDNA targets synthesised following Nugen protocol from different amounts of input RNA . Bioanalyzer electrophoretic profiles of cDNA targets obtained after the amplification of 50 pg, 100 pg, 250 pg, 500 pg, and 1 ng of total RNA using Nugen chemistry.
    Figure Legend Snippet: Quality of cDNA targets synthesised following Nugen protocol from different amounts of input RNA . Bioanalyzer electrophoretic profiles of cDNA targets obtained after the amplification of 50 pg, 100 pg, 250 pg, 500 pg, and 1 ng of total RNA using Nugen chemistry.

    Techniques Used: Amplification

    Quality of input RNA and targets synthesised from different amounts of input RNA . A , Bioanalyzer electrophoretic profile of the diluted Universal Human Reference RNA used as input for all amplifications. This profile corresponds to a classical and non-degraded human RNA with two fine characteristic peaks corresponding to 18S and 28S RNAs. 8.4 corresponds to the RNA Integrity Number (RIN) and reflects the high quality of this RNA B , electrophoretic profiles of cRNA obtained after one- and two-round Affymetrix amplification using 2 μg, and 100 ng of input RNA respectively or water as negative control. C , D , E , F , electrophoretic profiles of cRNA or cDNA obtained using Ambion ( C ), Arcturus ( D ), Epicentre ( E ) and Nugen ( F ) amplification systems from 250 pg and 500 pg of input RNA, or water as negative control.
    Figure Legend Snippet: Quality of input RNA and targets synthesised from different amounts of input RNA . A , Bioanalyzer electrophoretic profile of the diluted Universal Human Reference RNA used as input for all amplifications. This profile corresponds to a classical and non-degraded human RNA with two fine characteristic peaks corresponding to 18S and 28S RNAs. 8.4 corresponds to the RNA Integrity Number (RIN) and reflects the high quality of this RNA B , electrophoretic profiles of cRNA obtained after one- and two-round Affymetrix amplification using 2 μg, and 100 ng of input RNA respectively or water as negative control. C , D , E , F , electrophoretic profiles of cRNA or cDNA obtained using Ambion ( C ), Arcturus ( D ), Epicentre ( E ) and Nugen ( F ) amplification systems from 250 pg and 500 pg of input RNA, or water as negative control.

    Techniques Used: Amplification, Negative Control

    Related Articles

    Sequencing:

    Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system
    Article Snippet: .. RNA amplification and cDNA preparation To obtain enough cDNA for sequencing, the 16s and 23s rRNA depleted RNAs (sample 1 and 2) were amplified using Nugen WT-Ovation Pico RNA amplification system [ ]. ..

    Microarray:

    Article Title: Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology
    Article Snippet: .. This RNA was then converted into large quantities of single stranded DNA through the use of NuGEN WT-Ovation™ Pico RNA Amplification System, originally designed to process samples for microarray analysis, which yielded between 150–350 ng/µl of DNA with the total amount obtained ranging from 4.5 to 10.5 µg. .. This ssDNA was then converted into double stranded DNA using the WT-Ovation™ Exon module, originally designed for cDNA library synthesis, resulting in concentrations ranging between 300–500 ng/µl with the total amount obtained ranging from 9 to 15 µg.

    Article Title: IL-12 and type I interferon prolong the division of activated CD8 T cells by maintaining high-affinity IL-2 signaling in vivo
    Article Snippet: .. RNA for the microarray was processed using the NuGEN WT-Ovation Pico RNA Amplification System along with the NuGEN WT-Ovation Exon Module. .. Samples were hybridized and loaded onto Affymetrix GeneChip Mouse GENE 1.0 ST arrays.

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8+ T cell differentiation
    Article Snippet: .. RNA for the microarray was processed using the NuGEN WT-Ovation Pico RNA Amplification System along with the NuGEN WT-Ovation Exon Module. .. Samples were hybridized and loaded onto Affymetrix GeneChip Mouse GENE 1.0 ST arrays.

    Amplification:

    Article Title: Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology
    Article Snippet: .. This RNA was then converted into large quantities of single stranded DNA through the use of NuGEN WT-Ovation™ Pico RNA Amplification System, originally designed to process samples for microarray analysis, which yielded between 150–350 ng/µl of DNA with the total amount obtained ranging from 4.5 to 10.5 µg. .. This ssDNA was then converted into double stranded DNA using the WT-Ovation™ Exon module, originally designed for cDNA library synthesis, resulting in concentrations ranging between 300–500 ng/µl with the total amount obtained ranging from 9 to 15 µg.

    Article Title: Virus-induced inflammasome activation is suppressed by prostaglandin D2/DP1 signaling
    Article Snippet: .. RNA was assessed for purity and quality using an Agilent 2100 Bioanalyzer and was processed using a NuGEN WT-Ovation Pico RNA Amplification System together with a NuGEN WT-Ovation Exon Module. .. Samples were hybridized and loaded onto Gene Chip mouse gene 2.0 ST arrays (Affymetrix).

    Article Title: Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1 [C]Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1 [C] [OA]
    Article Snippet: .. RNA was quantified with a Bioanalyzer (Agilent Technologies) and amplified and labeled with the WT-Ovation Pico RNA Amplification System and FL-Ovation cDNA Biotin Module V2, respectively (NuGEN). .. The labeled cDNA was hybridized, washed, and stained on an ATH-121501 Arabidopsis full-genome microarray using a Hybridization Control Kit, a GeneChip Hybridization, Wash, and Stain Kit, a GeneChip Fluidics Station 450, and a GeneChip Scanner (Affymetrix).

    Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system
    Article Snippet: .. RNA amplification and cDNA preparation To obtain enough cDNA for sequencing, the 16s and 23s rRNA depleted RNAs (sample 1 and 2) were amplified using Nugen WT-Ovation Pico RNA amplification system [ ]. ..

    Article Title: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling
    Article Snippet: .. In contrast with other manufacturer protocols, the WT-Ovation™ Pico RNA Amplification System (Nugen) is not based on T7 polymerase cRNA synthesis. .. Nugen has designed a technique called Ribo-SPIA™, which is a three-step process that generates amplified cDNA from as little as 500 picograms of total RNA[ ].

    Article Title: Osteocalcin Expression by Circulating Endothelial Progenitor Cells in Patients with Coronary Atherosclerosis
    Article Snippet: .. Because the overall number of the CD34+ cells was limited and therefore the yield of total RNA was low, we used the WT-Ovation™ Pico RNA amplification system (NuGEN, Technologies, Inc) to synthesize micrograms quantities of amplified cDNA starting with total RNA input amounts of 50 ng for all the samples. .. In this linear amplification system, the relative representation of each transcript species in the original sample is maintained during and after amplification.

    Article Title: IL-12 and type I interferon prolong the division of activated CD8 T cells by maintaining high-affinity IL-2 signaling in vivo
    Article Snippet: .. RNA for the microarray was processed using the NuGEN WT-Ovation Pico RNA Amplification System along with the NuGEN WT-Ovation Exon Module. .. Samples were hybridized and loaded onto Affymetrix GeneChip Mouse GENE 1.0 ST arrays.

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8+ T cell differentiation
    Article Snippet: .. RNA for the microarray was processed using the NuGEN WT-Ovation Pico RNA Amplification System along with the NuGEN WT-Ovation Exon Module. .. Samples were hybridized and loaded onto Affymetrix GeneChip Mouse GENE 1.0 ST arrays.

    Labeling:

    Article Title: Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1 [C]Positive Fluorescent Selection Permits Precise, Rapid, and In-Depth Overexpression Analysis in Plant Protoplasts 1 [C] [OA]
    Article Snippet: .. RNA was quantified with a Bioanalyzer (Agilent Technologies) and amplified and labeled with the WT-Ovation Pico RNA Amplification System and FL-Ovation cDNA Biotin Module V2, respectively (NuGEN). .. The labeled cDNA was hybridized, washed, and stained on an ATH-121501 Arabidopsis full-genome microarray using a Hybridization Control Kit, a GeneChip Hybridization, Wash, and Stain Kit, a GeneChip Fluidics Station 450, and a GeneChip Scanner (Affymetrix).

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  • About
  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • wt ovation pico rna amplification system  (Nugen)
    92
    Nugen wt ovation pico rna amplification system
    Removal of 16s and <t>23s</t> rRNAs using MICROB Express ™ kit from Ambion . Total <t>RNA</t> samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.
    Wt Ovation Pico Rna Amplification System, supplied by Nugen, used in various techniques. Bioz Stars score: 92/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt ovation pico rna amplification system/product/Nugen
    Average 92 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    wt ovation pico rna amplification system - by Bioz Stars, 2021-01
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Removal of 16s and 23s rRNAs using MICROB Express ™ kit from Ambion . Total RNA samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.

    Journal: BMC Microbiology

    Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system

    doi: 10.1186/1471-2180-8-72

    Figure Lengend Snippet: Removal of 16s and 23s rRNAs using MICROB Express ™ kit from Ambion . Total RNA samples before (in red) and after (in blue) rRNA depletion were analyzed on the Agilent 2100 Bioanalyzer.

    Article Snippet: RNA amplification and cDNA preparation To obtain enough cDNA for sequencing, the 16s and 23s rRNA depleted RNAs (sample 1 and 2) were amplified using Nugen WT-Ovation Pico RNA amplification system [ ].

    Techniques:

    RT-PCR of 20 gene candidates . Lane 1: low molecular weight DNA ladder from New England Biolabs. Size range: 25 bp to 766 bp. Lane 2-21: 20 gene candidates, VBISMa0080, VBISMa0492, VBISMa1337, VBISMb0078, VBISMb0839, VBISMc0095, VBISMc0802, VBISMc1000, VBISMc1221, VBISMc1492, VBISMc1793, VBISMc2171, VBISMc2174, VBISMc2596, VBISMc2940, VBISMc2955, VBISMc3188, VBISMc3282, VBISMc4046 and VBISMc4289, respectively. For majority RT-PCR reactions, each produced one corresponding PCR product (lane 3-5, 7, 9-12, 14-19 and 21). These PCR products were directly sequenced and their sequences matched to the corresponding gene candidates. Multiple PCR products were found in lane 2, 6, 8, 13 and 20. The bands with the correct PCR product sizes are labeled with *. These PCR products were used to do a second round of PCR to produce enough DNA for sequencing. The sequencing results confirmed that they matched to the corresponding gene candidates. The most abundant PCR product in lane 2 was sequenced and determined to be a part of 23s rRNA sequence. Lane 22-29: negative controls using the RNA sample that was not reverse transcribed and primer pairs of new genes to show no genomic DNA contamination. In each lane of 22 to 29, combined primer pairs of two or three genes were used. Lane 30: no template control. Primer pairs of cm0012a, cm012b and cm016a, cm016b were used.

    Journal: BMC Microbiology

    Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system

    doi: 10.1186/1471-2180-8-72

    Figure Lengend Snippet: RT-PCR of 20 gene candidates . Lane 1: low molecular weight DNA ladder from New England Biolabs. Size range: 25 bp to 766 bp. Lane 2-21: 20 gene candidates, VBISMa0080, VBISMa0492, VBISMa1337, VBISMb0078, VBISMb0839, VBISMc0095, VBISMc0802, VBISMc1000, VBISMc1221, VBISMc1492, VBISMc1793, VBISMc2171, VBISMc2174, VBISMc2596, VBISMc2940, VBISMc2955, VBISMc3188, VBISMc3282, VBISMc4046 and VBISMc4289, respectively. For majority RT-PCR reactions, each produced one corresponding PCR product (lane 3-5, 7, 9-12, 14-19 and 21). These PCR products were directly sequenced and their sequences matched to the corresponding gene candidates. Multiple PCR products were found in lane 2, 6, 8, 13 and 20. The bands with the correct PCR product sizes are labeled with *. These PCR products were used to do a second round of PCR to produce enough DNA for sequencing. The sequencing results confirmed that they matched to the corresponding gene candidates. The most abundant PCR product in lane 2 was sequenced and determined to be a part of 23s rRNA sequence. Lane 22-29: negative controls using the RNA sample that was not reverse transcribed and primer pairs of new genes to show no genomic DNA contamination. In each lane of 22 to 29, combined primer pairs of two or three genes were used. Lane 30: no template control. Primer pairs of cm0012a, cm012b and cm016a, cm016b were used.

    Article Snippet: RNA amplification and cDNA preparation To obtain enough cDNA for sequencing, the 16s and 23s rRNA depleted RNAs (sample 1 and 2) were amplified using Nugen WT-Ovation Pico RNA amplification system [ ].

    Techniques: Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Produced, Polymerase Chain Reaction, Labeling, Sequencing

    Flowchart of experimental methodology. Cell culture supernatants containing viral particles was collected from HIV infected cells. The RNA was extracted using the QIAamp Viral RNA Mini Kit and coverted into large quantities of single stranded DNA using the WT-Ovation Pico RNA Amplification System. The complementary strand for the ssDNA was then synthesized using the WT-Ovation Exon Module. The final step in the process involved using the Genomic DNA Sample Prep Kit to produce an Illumina library.

    Journal: PLoS ONE

    Article Title: Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology

    doi: 10.1371/journal.pone.0013564

    Figure Lengend Snippet: Flowchart of experimental methodology. Cell culture supernatants containing viral particles was collected from HIV infected cells. The RNA was extracted using the QIAamp Viral RNA Mini Kit and coverted into large quantities of single stranded DNA using the WT-Ovation Pico RNA Amplification System. The complementary strand for the ssDNA was then synthesized using the WT-Ovation Exon Module. The final step in the process involved using the Genomic DNA Sample Prep Kit to produce an Illumina library.

    Article Snippet: This RNA was then converted into large quantities of single stranded DNA through the use of NuGEN WT-Ovation™ Pico RNA Amplification System, originally designed to process samples for microarray analysis, which yielded between 150–350 ng/µl of DNA with the total amount obtained ranging from 4.5 to 10.5 µg.

    Techniques: Cell Culture, Infection, Amplification, Synthesized, Sample Prep

    Reproducibility of expression data obtained using Nugen chemistry across amounts of input RNA . Pearson's correlation coefficients were computed and represented graphically for each pairwise comparison of assays. Lower correlations were observed for data obtained from smallest amounts of input RNA (50 pg and 100 pg).

    Journal: BMC Genomics

    Article Title: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

    doi: 10.1186/1471-2164-10-246

    Figure Lengend Snippet: Reproducibility of expression data obtained using Nugen chemistry across amounts of input RNA . Pearson's correlation coefficients were computed and represented graphically for each pairwise comparison of assays. Lower correlations were observed for data obtained from smallest amounts of input RNA (50 pg and 100 pg).

    Article Snippet: In contrast with other manufacturer protocols, the WT-Ovation™ Pico RNA Amplification System (Nugen) is not based on T7 polymerase cRNA synthesis.

    Techniques: Expressing

    Quality of cDNA targets synthesised following Nugen protocol from different amounts of input RNA . Bioanalyzer electrophoretic profiles of cDNA targets obtained after the amplification of 50 pg, 100 pg, 250 pg, 500 pg, and 1 ng of total RNA using Nugen chemistry.

    Journal: BMC Genomics

    Article Title: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

    doi: 10.1186/1471-2164-10-246

    Figure Lengend Snippet: Quality of cDNA targets synthesised following Nugen protocol from different amounts of input RNA . Bioanalyzer electrophoretic profiles of cDNA targets obtained after the amplification of 50 pg, 100 pg, 250 pg, 500 pg, and 1 ng of total RNA using Nugen chemistry.

    Article Snippet: In contrast with other manufacturer protocols, the WT-Ovation™ Pico RNA Amplification System (Nugen) is not based on T7 polymerase cRNA synthesis.

    Techniques: Amplification

    Quality of input RNA and targets synthesised from different amounts of input RNA . A , Bioanalyzer electrophoretic profile of the diluted Universal Human Reference RNA used as input for all amplifications. This profile corresponds to a classical and non-degraded human RNA with two fine characteristic peaks corresponding to 18S and 28S RNAs. 8.4 corresponds to the RNA Integrity Number (RIN) and reflects the high quality of this RNA B , electrophoretic profiles of cRNA obtained after one- and two-round Affymetrix amplification using 2 μg, and 100 ng of input RNA respectively or water as negative control. C , D , E , F , electrophoretic profiles of cRNA or cDNA obtained using Ambion ( C ), Arcturus ( D ), Epicentre ( E ) and Nugen ( F ) amplification systems from 250 pg and 500 pg of input RNA, or water as negative control.

    Journal: BMC Genomics

    Article Title: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

    doi: 10.1186/1471-2164-10-246

    Figure Lengend Snippet: Quality of input RNA and targets synthesised from different amounts of input RNA . A , Bioanalyzer electrophoretic profile of the diluted Universal Human Reference RNA used as input for all amplifications. This profile corresponds to a classical and non-degraded human RNA with two fine characteristic peaks corresponding to 18S and 28S RNAs. 8.4 corresponds to the RNA Integrity Number (RIN) and reflects the high quality of this RNA B , electrophoretic profiles of cRNA obtained after one- and two-round Affymetrix amplification using 2 μg, and 100 ng of input RNA respectively or water as negative control. C , D , E , F , electrophoretic profiles of cRNA or cDNA obtained using Ambion ( C ), Arcturus ( D ), Epicentre ( E ) and Nugen ( F ) amplification systems from 250 pg and 500 pg of input RNA, or water as negative control.

    Article Snippet: In contrast with other manufacturer protocols, the WT-Ovation™ Pico RNA Amplification System (Nugen) is not based on T7 polymerase cRNA synthesis.

    Techniques: Amplification, Negative Control