wt ngf  (Alomone Labs)


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    Structured Review

    Alomone Labs wt ngf
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Wt Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt ngf - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags"

    Article Title: Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113708

    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Figure Legend Snippet: A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.

    Techniques Used: Western Blot, In Vitro, Purification, Differentiation Assay

    wt ngf  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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    Structured Review

    Alomone Labs wt ngf
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Wt Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt ngf - by Bioz Stars, 2023-06
    93/100 stars

    Images

    1) Product Images from "Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags"

    Article Title: Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113708

    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Figure Legend Snippet: A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.

    Techniques Used: Western Blot, In Vitro, Purification, Differentiation Assay

    pro ngf wt h  (Alomone Labs)


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    • 93
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    Structured Review

    Alomone Labs pro ngf wt h
    Pro Ngf Wt H, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro ngf wt h/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro ngf wt h - by Bioz Stars, 2023-06
    93/100 stars

    Images

    recombinant pro ngf wt h  (Alomone Labs)


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    • 93
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    Structured Review

    Alomone Labs recombinant pro ngf wt h
    Recombinant Pro Ngf Wt H, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant pro ngf wt h/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant pro ngf wt h - by Bioz Stars, 2023-06
    93/100 stars

    Images

    wt h pro ngf  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
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    • 93
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    Structured Review

    Alomone Labs wt h pro ngf
    Wt H Pro Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt h pro ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt h pro ngf - by Bioz Stars, 2023-06
    93/100 stars

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    • wt ngf  (Alomone Labs)
    93
    Alomone Labs wt ngf
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Wt Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt ngf - by Bioz Stars, 2023-06
    93/100 stars
    		  Buy from Supplier
    pro ngf wt h  (Alomone Labs)
    93
    Alomone Labs pro ngf wt h
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Pro Ngf Wt H, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro ngf wt h/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro ngf wt h - by Bioz Stars, 2023-06
    93/100 stars
    		  Buy from Supplier
    recombinant pro ngf wt h  (Alomone Labs)
    93
    Alomone Labs recombinant pro ngf wt h
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Recombinant Pro Ngf Wt H, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant pro ngf wt h/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant pro ngf wt h - by Bioz Stars, 2023-06
    93/100 stars
    		  Buy from Supplier
    wt h pro ngf  (Alomone Labs)
    93
    Alomone Labs wt h pro ngf
    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified <t>NGF-A4</t> ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay <t>in</t> <t>PC12</t> cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.
    Wt H Pro Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt h pro ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt h pro ngf - by Bioz Stars, 2023-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.

    Journal: PLoS ONE

    Article Title: Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags

    doi: 10.1371/journal.pone.0113708

    Figure Lengend Snippet: A–B ) Western blot for the analysis of the in vitro biotinylation reaction of purified NGF-A4 ( A ) and proNGF-A4 ( B ) using CoA-biotin substrate and AcpS or SfpS PPTases. The same biotinylation reaction is performed in parallel using untagged wt NGF and wt proNGF as negative controls. Streptavidin-HRP is used for detection of biotin. The anti-NGF blot is the loading control. C ) Typical DIC images obtained when performing the differentiation assay in PC12 cells using ∼50 ng/ml of wt NGF, NGF-A4 and biotinylated NGF-A4 (NGF-A4b). Untreated cells are the control. Scale bar: 20 µm.

    Article Snippet: PC12 differentiation was induced by treatment with ∼50 ng/ml wt NGF (Alomone Labs), recombinant NGF-A4 or biotinylated NGF-A4 (NGF-A4b); cells were observed after five days at a Leica DM6000 microscope capable of transmission DIC imaging.

    Techniques: Western Blot, In Vitro, Purification, Differentiation Assay