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Charles River Laboratories wt mice
Wt Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wt mice/product/Charles River Laboratories
Average 92 stars, based on 141 article reviews
Price from $9.99 to $1999.99
wt mice - by Bioz Stars, 2021-01
92/100 stars

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Mouse Assay:

Article Title: Chikungunya virus-induced autophagy delays caspase-dependent cell death
Article Snippet: .. WT mice were obtained from Charles River or The Jackson Laboratory. .. Atg16LHM mice on the C57BL/6 background, fully backcrossed by speed congenics, were generated by H. Virgin (Washington University School of Medicine, St. Louis, MO) and have been previously described ( ).

Article Title: A tumor-myeloid cell axis, mediated via interleukin 1α and TSLP, promotes breast cancer progression
Article Snippet: .. CD45.2+ WT mice were purchased from Charles River Laboratories. .. TSLP-KO , and SPC-TSLP mice were all backcrossed to BALB/c background for more than 10 generations.

Article Title: DNase Sda1 Allows Invasive M1T1 Group A Streptococcus to Prevent TLR9-Dependent Recognition
Article Snippet: .. C57BL/6 TLR9-deficient mice were originally developed by Dr. Shizuo Akira (Osaka University, Japan); WT mice were purchased from Charles River Laboratories. ..

Article Title: Regulation of the effector function of CD8+ T cells by gut microbiota-derived metabolite butyrate
Article Snippet: .. WT mice were obtained from Charles River Laboratories. .. Ffar2 −/− Ffar3 −/− mice were generously provided by Dr. Stefan Offermanns (Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany).

Article Title: STAT1-Dependent Signal Integration between IFNγ and TLR4 in Vascular Cells Reflect Pro-Atherogenic Responses in Human Atherosclerosis
Article Snippet: .. WT mice (strain background C57BL/6 ) were obtained from Charles River Laboratories. .. STAT1−/− and IRF8−/− mice (both C57BL/6 background) were kindly provided by Thomas Decker and Carol Stocking, respectively .

Article Title: Breeding Strategy Determines Rupture Incidence in Post-Infarct Healing WARPing Cardiovascular Research
Article Snippet: .. Finally, an MI study using 9 in-house bred WT mice, 15 WT mice purchased from Harlan and 16 WT mice purchased from Charles River (6 to 12 weeks) was performed as in the first experiments and mice were sacrificed after 14 days. .. Humane endpoints were used during all experiments: animals were monitored daily for grade of activity, healing of the surgical wound, weight loss, normal breathing and absence of ruffled fur, but no mice needed to be sacrificed prior to experimental endpoints.

Article Title: Defective NOD2 peptidoglycan sensing promotes diet-induced inflammation, dysbiosis, and insulin resistance
Article Snippet: .. WT mice were from Charles River (Sherbrooke, Quebec, Canada or St Germain sur l'Arbresle, France) or in-house littermates, as indicated. ..

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  • 92
    Charles River Laboratories wt c57bl 6 mice
    Effect of LTC 4 on eosinophilic pulmonary inflammation and platelet activation. OVA-sensitized <t>C57BL/6</t> were challenged with 0.1% OVA on three successive days, with or without inhalation of LTC 4 (2.2 nmol) 12 h before each challenge. BAL fluid was collected
    Wt C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt c57bl 6 mice/product/Charles River Laboratories
    Average 92 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    wt c57bl 6 mice - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    88
    Charles River Laboratories littermate wild type wt mice
    Steady-state levels of 8-oxodG and expression of the p53R2 protein in brain areas of <t>wild-type</t> and R6/2 <t>mice.</t> Groups of <t>WT</t> (n = 10) and R6/2 mice (n = 10) were sacrificed and levels of 8-oxo-dG were determined by HPLC-EC in genomic DNA prepared from the indicated brain areas. DNA 8-oxodG levels in ( A ) striatum and ( B ) motor cortex of 12 week-old WT (WT, open bar) and R6/2 mice (grey ba r ). Data are the mean±SE. The asterisks indicate significant differences by Student's t -test (* P
    Littermate Wild Type Wt Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/littermate wild type wt mice/product/Charles River Laboratories
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    littermate wild type wt mice - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    86
    Charles River Laboratories wt fvb n female mice
    Kinetics of G-CSF stimulation reveal G-CSF targets expansion of HSC and MPP populations in the BM. ( A and B ) BM, blood, spleen, lung, and MG (tumor) were harvested from WT <t>FVB/n</t> and PyMT mice (14–15 wk) or anti–G-CSF–treated mice. ( A ) Quantification of total BM cells ( Left ), cell numbers of Ly6G + and Ly6C hi cells ( Center ), and cell numbers of HSC, MPP F+ , and MPP F- populations ( Right ) in BM. ( B ) Representative images of femurs and tibias ( Left ) and spleens ( Right ). ( C–H ) BM, spleen, and blood were harvested from WT and G-CSF–stimulated (0.5–5 consecutive days) FVB/n mice. ( C ) Summary of the frequency (% total) of CD11b + Gr1 + cells assessed by flow cytometry. ( D ) FACS plots illustrate the frequency (% of total) of Ly6G + cells in BM, spleen, and blood ( Left ), histograms of Ly6G expression in Ly6G + cells ( Center ), and total cell numbers of Ly6G + cells ( Right ). ( E ) Cell numbers of CMPs, GMPs, and MEPs in BM. ( F ) Representative images of femurs and tibias. Cell numbers of ( G ) HSCs, MPPs F+ , and MPPs F- in BM and ( H ) HSCs, MPPs F+ , and MPPs F- in spleen. Data are representative of ( A and B ) three experiments (mean ± SEM, n = 3–7) and ( C–H ) two experiments (mean ± SEM, n = 3–10). * P
    Wt Fvb N Female Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt fvb n female mice/product/Charles River Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wt fvb n female mice - by Bioz Stars, 2021-01
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    Image Search Results


    Effect of LTC 4 on eosinophilic pulmonary inflammation and platelet activation. OVA-sensitized C57BL/6 were challenged with 0.1% OVA on three successive days, with or without inhalation of LTC 4 (2.2 nmol) 12 h before each challenge. BAL fluid was collected

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: 1Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors

    doi: 10.4049/jimmunol.1402959

    Figure Lengend Snippet: Effect of LTC 4 on eosinophilic pulmonary inflammation and platelet activation. OVA-sensitized C57BL/6 were challenged with 0.1% OVA on three successive days, with or without inhalation of LTC 4 (2.2 nmol) 12 h before each challenge. BAL fluid was collected

    Article Snippet: WT C57BL/6 mice were purchased from Charles River.

    Techniques: Activation Assay

    Effect of TP receptor deletion on LTC 4 -mediated eosinophil recruitment to the lung. A. Cytofluorographic detection of eosinophils and neutrophils (based on light scatter characteristics within the CD45+ gate) in the blood of representative C57BL/6 WT

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: 1Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors

    doi: 10.4049/jimmunol.1402959

    Figure Lengend Snippet: Effect of TP receptor deletion on LTC 4 -mediated eosinophil recruitment to the lung. A. Cytofluorographic detection of eosinophils and neutrophils (based on light scatter characteristics within the CD45+ gate) in the blood of representative C57BL/6 WT

    Article Snippet: WT C57BL/6 mice were purchased from Charles River.

    Techniques:

    Subcutaneous and perigonadal MMEKO preadipocytes show impaired insulin signaling . MMEKO and control (wild-type C57BL/6) mice aged 12 weeks were sacrificed for in vitro insulin signaling. The stromal vascular cells (preadipocytes) from the subcutaneous (inguinal) and visceral (perigonadal) adipose depots were isolated. Cells were serum-starved for 4 h before a 20-minute insulin stimulation followed by protein isolation and western blot. (A) Western blot of protein of subcutaneous and perigonadal MMEKO preadipocytes showed impaired response among different insulin-signaling proteins. (B–I) Densitometric analysis of protein levels. The levels of unphosphorylated proteins were grouped by adipose depot (PG = Perigondal, SC = Subcutaneous) and genotype (black = Control, grey = MMEKO). The levels of phosphorylated proteins were grouped by adipose depot, genotype, and insulin stimulation (– = no insulin, + = 20 min after 100 μM insulin). Asterisks indicate p ≤ 0.05 by Student's t -test. N = 3. Bars indicate mean ± s.e.m.

    Journal: Molecular Metabolism

    Article Title: Membrane metallo-endopeptidase (Neprilysin) regulates inflammatory response and insulin signaling in white preadipocytes

    doi: 10.1016/j.molmet.2019.01.006

    Figure Lengend Snippet: Subcutaneous and perigonadal MMEKO preadipocytes show impaired insulin signaling . MMEKO and control (wild-type C57BL/6) mice aged 12 weeks were sacrificed for in vitro insulin signaling. The stromal vascular cells (preadipocytes) from the subcutaneous (inguinal) and visceral (perigonadal) adipose depots were isolated. Cells were serum-starved for 4 h before a 20-minute insulin stimulation followed by protein isolation and western blot. (A) Western blot of protein of subcutaneous and perigonadal MMEKO preadipocytes showed impaired response among different insulin-signaling proteins. (B–I) Densitometric analysis of protein levels. The levels of unphosphorylated proteins were grouped by adipose depot (PG = Perigondal, SC = Subcutaneous) and genotype (black = Control, grey = MMEKO). The levels of phosphorylated proteins were grouped by adipose depot, genotype, and insulin stimulation (– = no insulin, + = 20 min after 100 μM insulin). Asterisks indicate p ≤ 0.05 by Student's t -test. N = 3. Bars indicate mean ± s.e.m.

    Article Snippet: MMEKO mice were bred with WT C57BL/6 mice from Charles River Laboratories, and F2 homozygous male KO mice were used for subsequent experiments.

    Techniques: Mouse Assay, In Vitro, Isolation, Western Blot

    Steady-state levels of 8-oxodG and expression of the p53R2 protein in brain areas of wild-type and R6/2 mice. Groups of WT (n = 10) and R6/2 mice (n = 10) were sacrificed and levels of 8-oxo-dG were determined by HPLC-EC in genomic DNA prepared from the indicated brain areas. DNA 8-oxodG levels in ( A ) striatum and ( B ) motor cortex of 12 week-old WT (WT, open bar) and R6/2 mice (grey ba r ). Data are the mean±SE. The asterisks indicate significant differences by Student's t -test (* P

    Journal: Nucleic Acids Research

    Article Title: Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine to induce CAG/CTG repeat instability

    doi: 10.1093/nar/gkw170

    Figure Lengend Snippet: Steady-state levels of 8-oxodG and expression of the p53R2 protein in brain areas of wild-type and R6/2 mice. Groups of WT (n = 10) and R6/2 mice (n = 10) were sacrificed and levels of 8-oxo-dG were determined by HPLC-EC in genomic DNA prepared from the indicated brain areas. DNA 8-oxodG levels in ( A ) striatum and ( B ) motor cortex of 12 week-old WT (WT, open bar) and R6/2 mice (grey ba r ). Data are the mean±SE. The asterisks indicate significant differences by Student's t -test (* P

    Article Snippet: Mice A colony of R6/2 ( ) transgenic and littermate wild-type (WT) mice was maintained at Charles River Laboratories (Calco, Italy).

    Techniques: Expressing, Mouse Assay, High Performance Liquid Chromatography

    Kinetics of G-CSF stimulation reveal G-CSF targets expansion of HSC and MPP populations in the BM. ( A and B ) BM, blood, spleen, lung, and MG (tumor) were harvested from WT FVB/n and PyMT mice (14–15 wk) or anti–G-CSF–treated mice. ( A ) Quantification of total BM cells ( Left ), cell numbers of Ly6G + and Ly6C hi cells ( Center ), and cell numbers of HSC, MPP F+ , and MPP F- populations ( Right ) in BM. ( B ) Representative images of femurs and tibias ( Left ) and spleens ( Right ). ( C–H ) BM, spleen, and blood were harvested from WT and G-CSF–stimulated (0.5–5 consecutive days) FVB/n mice. ( C ) Summary of the frequency (% total) of CD11b + Gr1 + cells assessed by flow cytometry. ( D ) FACS plots illustrate the frequency (% of total) of Ly6G + cells in BM, spleen, and blood ( Left ), histograms of Ly6G expression in Ly6G + cells ( Center ), and total cell numbers of Ly6G + cells ( Right ). ( E ) Cell numbers of CMPs, GMPs, and MEPs in BM. ( F ) Representative images of femurs and tibias. Cell numbers of ( G ) HSCs, MPPs F+ , and MPPs F- in BM and ( H ) HSCs, MPPs F+ , and MPPs F- in spleen. Data are representative of ( A and B ) three experiments (mean ± SEM, n = 3–7) and ( C–H ) two experiments (mean ± SEM, n = 3–10). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils

    doi: 10.1073/pnas.1424927112

    Figure Lengend Snippet: Kinetics of G-CSF stimulation reveal G-CSF targets expansion of HSC and MPP populations in the BM. ( A and B ) BM, blood, spleen, lung, and MG (tumor) were harvested from WT FVB/n and PyMT mice (14–15 wk) or anti–G-CSF–treated mice. ( A ) Quantification of total BM cells ( Left ), cell numbers of Ly6G + and Ly6C hi cells ( Center ), and cell numbers of HSC, MPP F+ , and MPP F- populations ( Right ) in BM. ( B ) Representative images of femurs and tibias ( Left ) and spleens ( Right ). ( C–H ) BM, spleen, and blood were harvested from WT and G-CSF–stimulated (0.5–5 consecutive days) FVB/n mice. ( C ) Summary of the frequency (% total) of CD11b + Gr1 + cells assessed by flow cytometry. ( D ) FACS plots illustrate the frequency (% of total) of Ly6G + cells in BM, spleen, and blood ( Left ), histograms of Ly6G expression in Ly6G + cells ( Center ), and total cell numbers of Ly6G + cells ( Right ). ( E ) Cell numbers of CMPs, GMPs, and MEPs in BM. ( F ) Representative images of femurs and tibias. Cell numbers of ( G ) HSCs, MPPs F+ , and MPPs F- in BM and ( H ) HSCs, MPPs F+ , and MPPs F- in spleen. Data are representative of ( A and B ) three experiments (mean ± SEM, n = 3–7) and ( C–H ) two experiments (mean ± SEM, n = 3–10). * P

    Article Snippet: WT FVB/n female mice were purchased from Charles River and WT C57BL/6 female (CD45.1 or CD45.2) mice were purchased from the Jackson (JAX) Laboratories for cross-breeding, in vivo G-CSF stimulation, or BM transplants.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, FACS, Expressing

    Breast cancer development results in profound remodeling of BM hematopoiesis. ( A ) Gating strategy to evaluate BrdU incorporation in Ly6G + cells by flow cytometry. ( B ) Representative FACS plots of BrdU incorporation (2 and 24 h after i.p. injection) in Ly6G + cells in BM and spleen of WT and PyMT FVB/n mice (14–15 wk). ( C ) Summary of the % of Ly6G + cells that are BrdU + in BM and spleen at 2 and 24 h. ( D ) Frequency (% of total) of CD11b + Gr1 + cells in BM of WT and PyMT FVB/n mice during tumor progression (8–15 wk). ( E ) Representative image of femurs and tibias from WT and PyMT FVB/n mice. ( F ) Schematic of myeloid differentiation. ( G–I ) The frequency and total cell numbers of MPs and HSPCs were evaluated in BM of WT and PyMT FVB/n mice (14–15 wk) by flow cytometry. ( G ) FACS plots illustrate the gating for MP, MPP, and HSC populations. Values shown are the frequency of cells within each parent gate. ( H ) Total cell numbers of MP subpopulations (CMP, GMP, and MEP). ( I ) Total cell numbers of HSCs and MPPs (MPP F- and MPP F+ ) in BM of WT and PyMT FVB/n mice (8–12 wk). Data are representative of ( A–C ) two experiments (mean ± SD, n = 3–4), ( D ) four experiments at each time point (mean ± SD, n = 10–12), ( E ) six experiments ( n = 8–15), ( H ) three experiments (mean ± SD, n = 5–7), ( I ) three experiments (8 and 10 wk; mean ± SD, n = 5), two experiments (12 wk; mean ± SD, n = 6–7), and five experiments (14–15 weeks; mean ± SD, n = 5–9). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils

    doi: 10.1073/pnas.1424927112

    Figure Lengend Snippet: Breast cancer development results in profound remodeling of BM hematopoiesis. ( A ) Gating strategy to evaluate BrdU incorporation in Ly6G + cells by flow cytometry. ( B ) Representative FACS plots of BrdU incorporation (2 and 24 h after i.p. injection) in Ly6G + cells in BM and spleen of WT and PyMT FVB/n mice (14–15 wk). ( C ) Summary of the % of Ly6G + cells that are BrdU + in BM and spleen at 2 and 24 h. ( D ) Frequency (% of total) of CD11b + Gr1 + cells in BM of WT and PyMT FVB/n mice during tumor progression (8–15 wk). ( E ) Representative image of femurs and tibias from WT and PyMT FVB/n mice. ( F ) Schematic of myeloid differentiation. ( G–I ) The frequency and total cell numbers of MPs and HSPCs were evaluated in BM of WT and PyMT FVB/n mice (14–15 wk) by flow cytometry. ( G ) FACS plots illustrate the gating for MP, MPP, and HSC populations. Values shown are the frequency of cells within each parent gate. ( H ) Total cell numbers of MP subpopulations (CMP, GMP, and MEP). ( I ) Total cell numbers of HSCs and MPPs (MPP F- and MPP F+ ) in BM of WT and PyMT FVB/n mice (8–12 wk). Data are representative of ( A–C ) two experiments (mean ± SD, n = 3–4), ( D ) four experiments at each time point (mean ± SD, n = 10–12), ( E ) six experiments ( n = 8–15), ( H ) three experiments (mean ± SD, n = 5–7), ( I ) three experiments (8 and 10 wk; mean ± SD, n = 5), two experiments (12 wk; mean ± SD, n = 6–7), and five experiments (14–15 weeks; mean ± SD, n = 5–9). * P

    Article Snippet: WT FVB/n female mice were purchased from Charles River and WT C57BL/6 female (CD45.1 or CD45.2) mice were purchased from the Jackson (JAX) Laboratories for cross-breeding, in vivo G-CSF stimulation, or BM transplants.

    Techniques: BrdU Incorporation Assay, Flow Cytometry, Cytometry, FACS, Injection, Mouse Assay

    G-CSF is sufficient for the enhanced ROS activity and Rb1 low phenotype of tumor-induced Ly6G + neutrophils. ( A ) Superoxide production was assessed by DHR fluorescence in Ly6G + cells in blood from WT and isotype or anti–G-CSF–treated PyMT FVB/n mice (14–15 wk). Representative FACS plots illustrate the frequency (% of total) of Ly6G + cells ( Left ), histogram illustrates DHR fluorescence in Ly6G + cells ( Center ), and bar graphs summarize the MFI of DHR in Ly6G + cells ( Right ). Multiplex laser capture technology (Eve Technologies) was used to measure the concentration of G-CSF in the serum from ( B ) WT, PyMT, and anti–G-CSF–treated PyMT FVB/n mice (14–15 wk) and ( C ) PBS control treated or G-CSF–stimulated (12 or 24 h) WT mice. ( D ) Superoxide production was assessed by DHR fluorescence in Ly6G + cells in blood from WT, G-CSF–stimulated, and PyMT FVB/n mice (14–15 wk). Representative FACS plots illustrate the frequency (% of total) of Ly6G + cells ( Left ), histogram illustrates DHR fluorescence in Ly6G + cells ( Center ), and bar graphs summarize the MFI of DHR in Ly6G + cells ( Right ). ( E and F ) Rb1 expression was assessed by Western blot in ( E ) total splenocytes and ( F ) total splenocytes ( Upper ) or FACS-sorted Ly6G + cells from the spleen ( Lower ). WT or PyMT FVB/n mice (14–15 wk). ( G ) FACS plots illustrate the frequency (% of total) of Ly6G + cells and histrograms illustrate intracellular Rb1 expression in Ly6G + cells, assessed by flow cytometry. Data are representative of ( A ) two experiments (mean ± SEM, n = 3–4), ( B ) two experiments (mean ± SEM, n = 6), ( C ) one experiment ( n = 2–4), ( D ) two experiments (mean ± SEM, n = 3–4), ( E and F ) two experiments ( n = 2–3 biological samples), and ( G ) two experiments ( n = 4). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils

    doi: 10.1073/pnas.1424927112

    Figure Lengend Snippet: G-CSF is sufficient for the enhanced ROS activity and Rb1 low phenotype of tumor-induced Ly6G + neutrophils. ( A ) Superoxide production was assessed by DHR fluorescence in Ly6G + cells in blood from WT and isotype or anti–G-CSF–treated PyMT FVB/n mice (14–15 wk). Representative FACS plots illustrate the frequency (% of total) of Ly6G + cells ( Left ), histogram illustrates DHR fluorescence in Ly6G + cells ( Center ), and bar graphs summarize the MFI of DHR in Ly6G + cells ( Right ). Multiplex laser capture technology (Eve Technologies) was used to measure the concentration of G-CSF in the serum from ( B ) WT, PyMT, and anti–G-CSF–treated PyMT FVB/n mice (14–15 wk) and ( C ) PBS control treated or G-CSF–stimulated (12 or 24 h) WT mice. ( D ) Superoxide production was assessed by DHR fluorescence in Ly6G + cells in blood from WT, G-CSF–stimulated, and PyMT FVB/n mice (14–15 wk). Representative FACS plots illustrate the frequency (% of total) of Ly6G + cells ( Left ), histogram illustrates DHR fluorescence in Ly6G + cells ( Center ), and bar graphs summarize the MFI of DHR in Ly6G + cells ( Right ). ( E and F ) Rb1 expression was assessed by Western blot in ( E ) total splenocytes and ( F ) total splenocytes ( Upper ) or FACS-sorted Ly6G + cells from the spleen ( Lower ). WT or PyMT FVB/n mice (14–15 wk). ( G ) FACS plots illustrate the frequency (% of total) of Ly6G + cells and histrograms illustrate intracellular Rb1 expression in Ly6G + cells, assessed by flow cytometry. Data are representative of ( A ) two experiments (mean ± SEM, n = 3–4), ( B ) two experiments (mean ± SEM, n = 6), ( C ) one experiment ( n = 2–4), ( D ) two experiments (mean ± SEM, n = 3–4), ( E and F ) two experiments ( n = 2–3 biological samples), and ( G ) two experiments ( n = 4). * P

    Article Snippet: WT FVB/n female mice were purchased from Charles River and WT C57BL/6 female (CD45.1 or CD45.2) mice were purchased from the Jackson (JAX) Laboratories for cross-breeding, in vivo G-CSF stimulation, or BM transplants.

    Techniques: Activity Assay, Fluorescence, Mouse Assay, FACS, Multiplex Assay, Concentration Assay, Expressing, Western Blot, Flow Cytometry, Cytometry

    Tumor-derived G-CSF, not GM-CSF or M-CSF, increases systemically during early tumor progression. Concentrations of G-CSF, GM-CSF, M-CSF, CXCL1, CCL2, CCL3, and CCL4 in ( A ) serum from WT and PyMT FVB/n mice (6–15 wk) and conditioned medium collected from ( B ) primary PyMT C57BL/6 tumor cells and ( C ) FVB/n VO-PyMT cell line were determined using multiplex laser bead technology (Eve Technologies). Data are representative of ( A ) two or three experiments (mean ± SEM, n = 4–7) and ( B and C ) two experiments, each sample in duplicate (mean ± SD of four biological samples). * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils

    doi: 10.1073/pnas.1424927112

    Figure Lengend Snippet: Tumor-derived G-CSF, not GM-CSF or M-CSF, increases systemically during early tumor progression. Concentrations of G-CSF, GM-CSF, M-CSF, CXCL1, CCL2, CCL3, and CCL4 in ( A ) serum from WT and PyMT FVB/n mice (6–15 wk) and conditioned medium collected from ( B ) primary PyMT C57BL/6 tumor cells and ( C ) FVB/n VO-PyMT cell line were determined using multiplex laser bead technology (Eve Technologies). Data are representative of ( A ) two or three experiments (mean ± SEM, n = 4–7) and ( B and C ) two experiments, each sample in duplicate (mean ± SD of four biological samples). * P

    Article Snippet: WT FVB/n female mice were purchased from Charles River and WT C57BL/6 female (CD45.1 or CD45.2) mice were purchased from the Jackson (JAX) Laboratories for cross-breeding, in vivo G-CSF stimulation, or BM transplants.

    Techniques: Derivative Assay, Mouse Assay, Multiplex Assay