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Proteintech anti wnt1
Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
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Proteintech wnt1
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech wnt1
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
86/100 stars

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Proteintech wnt inhibitory factor 1 wif1
Wnt Inhibitory Factor 1 Wif1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt inhibitory factor 1 wif1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt inhibitory factor 1 wif1 - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech anti wnt 1
Anti Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt 1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt 1 - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech anti wnt 1
Anti Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt 1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt 1 - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech antibodies recognizing wnt1
Effects of miR-34a on the <t>WNT1/β-catenin</t> axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.
Antibodies Recognizing Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies recognizing wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibodies recognizing wnt1 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke"

Article Title: Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

Journal: Neural Regeneration Research

doi: 10.4103/1673-5374.367831

Effects of miR-34a on the WNT1/β-catenin axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.
Figure Legend Snippet: Effects of miR-34a on the WNT1/β-catenin axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.

Techniques Used: Expressing, Binding Assay, Mutagenesis, Luciferase, Transfection, Activity Assay, Over Expression, Plasmid Preparation

Effects of WNT1 or SIRT1 suppression on apoptosis in miR-34a knockdown cell line. (A) Protein expression of WNT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (B) Protein expression of SIRT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (magnification ×400; scale bar: 50 μm; DAPI: blue; TUNEL: red) and quantitative analysis of TUNEL staining ( n = 3). TUNEL staining was performed to assess hDPSC apoptosis following OGD/R for 24 hours. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t- test). DAPI: 4′,6-Diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells.
Figure Legend Snippet: Effects of WNT1 or SIRT1 suppression on apoptosis in miR-34a knockdown cell line. (A) Protein expression of WNT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (B) Protein expression of SIRT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (magnification ×400; scale bar: 50 μm; DAPI: blue; TUNEL: red) and quantitative analysis of TUNEL staining ( n = 3). TUNEL staining was performed to assess hDPSC apoptosis following OGD/R for 24 hours. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t- test). DAPI: 4′,6-Diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells.

Techniques Used: Expressing, TUNEL Assay, Staining

Effects of WNT1 or SIRT1 suppression on the proliferation and antioxidant capacity of miR-34a knockdown in hDPSCs. Superoxide dismutase (SOD) activity assay and dihydroethidium (DHE) staining were performed to assess the antioxidant capacity of hDPSCs following oxygen-glucose deprivation/reoxygenation for 24 hours. (A) 5-ethynyl-2′-deoxyuridine (EdU) staining (scale bar: 20 μm; DAPI: blue, EdU: red) and quantitative analysis of EdU staining ( n = 3). (B) Cell viability ( n = 6). (C) Percentage of SOD activity ( n = 6). (D) DHE staining (magnification ×200; scale bar: 20 μm; DHE: red) and quantitative analysis of DHE staining ( n = 3). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t -test). OD: Optical density.
Figure Legend Snippet: Effects of WNT1 or SIRT1 suppression on the proliferation and antioxidant capacity of miR-34a knockdown in hDPSCs. Superoxide dismutase (SOD) activity assay and dihydroethidium (DHE) staining were performed to assess the antioxidant capacity of hDPSCs following oxygen-glucose deprivation/reoxygenation for 24 hours. (A) 5-ethynyl-2′-deoxyuridine (EdU) staining (scale bar: 20 μm; DAPI: blue, EdU: red) and quantitative analysis of EdU staining ( n = 3). (B) Cell viability ( n = 6). (C) Percentage of SOD activity ( n = 6). (D) DHE staining (magnification ×200; scale bar: 20 μm; DHE: red) and quantitative analysis of DHE staining ( n = 3). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t -test). OD: Optical density.

Techniques Used: Activity Assay, Staining


Structured Review

Proteintech wnt1
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wnt1 - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech anti wnt1
Phenotypes of patients with <t> WNT1 </t> mutations at baseline
Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti wnt1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti wnt1 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Genotypic and Phenotypic Spectrum and Pathogenesis of WNT1 Variants in a Large Cohort of Patients With OI/Osteoporosis"

Article Title: Genotypic and Phenotypic Spectrum and Pathogenesis of WNT1 Variants in a Large Cohort of Patients With OI/Osteoporosis

Journal: The Journal of Clinical Endocrinology and Metabolism

doi: 10.1210/clinem/dgac752

Phenotypes of patients with  WNT1  mutations at baseline
Figure Legend Snippet: Phenotypes of patients with WNT1 mutations at baseline

Techniques Used:

Efficacy of antiresorptive therapy in WNT1-mutant patients. A, Changes in bone mineral density (BMD) after zoledronic acid, alendronate, or denosumab treatment. FN, femoral neck; LS, lumbar spine; TH, total hip. Data are expressed as the median and interquartile range. The Wilcoxon paired test was performed to compare BMD at baseline and at final follow-up. ** P less than .01 vs baseline. B, Changes in bone turnover markers after zoledronic acid and alendronate treatment. ALP, alkaline phosphatase; β-CTX, cross-linked C-telopeptide of type I collagen. Data are expressed as the median and interquartile range. The Wilcoxon paired test was performed to compare ALP and β-CTX at baseline and at final follow-up. ** P less than .01 vs baseline.
Figure Legend Snippet: Efficacy of antiresorptive therapy in WNT1-mutant patients. A, Changes in bone mineral density (BMD) after zoledronic acid, alendronate, or denosumab treatment. FN, femoral neck; LS, lumbar spine; TH, total hip. Data are expressed as the median and interquartile range. The Wilcoxon paired test was performed to compare BMD at baseline and at final follow-up. ** P less than .01 vs baseline. B, Changes in bone turnover markers after zoledronic acid and alendronate treatment. ALP, alkaline phosphatase; β-CTX, cross-linked C-telopeptide of type I collagen. Data are expressed as the median and interquartile range. The Wilcoxon paired test was performed to compare ALP and β-CTX at baseline and at final follow-up. ** P less than .01 vs baseline.

Techniques Used: Mutagenesis

Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients with vertebral body reshaping after treatment. A 1 to D 1 , Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients at baseline. A 2 to D 2 , Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients after treatment. The white arrows indicate the compressed vertebra before and after treatment. Four patients had reshaping of compressed vertebral bodies after treatment. Patient 1 received denosumab treatment while the remaining 3 patients received zoledronic acid.
Figure Legend Snippet: Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients with vertebral body reshaping after treatment. A 1 to D 1 , Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients at baseline. A 2 to D 2 , Lateral x-ray films of the thoracolumbar spine of WNT1-mutant patients after treatment. The white arrows indicate the compressed vertebra before and after treatment. Four patients had reshaping of compressed vertebral bodies after treatment. Patient 1 received denosumab treatment while the remaining 3 patients received zoledronic acid.

Techniques Used: Mutagenesis

Spectrum of  WNT1  mutations in patients with osteogenesis imperfecta or early-onset osteoporosis
Figure Legend Snippet: Spectrum of WNT1 mutations in patients with osteogenesis imperfecta or early-onset osteoporosis

Techniques Used: Mutagenesis

Characteristics of osteogenesis imperfecta patients with biallelic nonsense mutations or frameshift mutations in  WNT1  from our study and previous studies
Figure Legend Snippet: Characteristics of osteogenesis imperfecta patients with biallelic nonsense mutations or frameshift mutations in WNT1 from our study and previous studies

Techniques Used: In Utero

Representative images of the undecalcified tibia of a patient with compound c.677C > T and c.502G > A WNT1 mutations. Left (3×): Goldner trichrome staining to evaluate osteoid and mature bone, scale bar = 500 μm. Arrows refer to osteoid, indicating active progression of bone formation that was not visible in tibial bone slices from patient 10. Right (40×): Toluidine blue staining to visualize osteoblasts and osteoclasts, scale bar = 20 μm. The underlying cement line was smooth in patient 10 and scalloped in the control. Several multinucleated osteoclasts and multiple osteoblasts (indicated by arrows) were found on the bone surfaces of the control bone.
Figure Legend Snippet: Representative images of the undecalcified tibia of a patient with compound c.677C > T and c.502G > A WNT1 mutations. Left (3×): Goldner trichrome staining to evaluate osteoid and mature bone, scale bar = 500 μm. Arrows refer to osteoid, indicating active progression of bone formation that was not visible in tibial bone slices from patient 10. Right (40×): Toluidine blue staining to visualize osteoblasts and osteoclasts, scale bar = 20 μm. The underlying cement line was smooth in patient 10 and scalloped in the control. Several multinucleated osteoclasts and multiple osteoblasts (indicated by arrows) were found on the bone surfaces of the control bone.

Techniques Used: Staining

Expression of β-catenin, WNT1, and collagen type I. A, Western blotting and semiquantitative analysis of β-catenin and WNT1 in bone samples from patient 10 and the control. Data were collected from 3 independent experiments. * P less than .05 vs the control. Statistical significance was assessed by a 2-tailed unpaired t test. B, Relative expression of COL1A1 in the fibroblasts from patient 10 and the control. Real-time quantitative polymerase chain reaction was performed to examine changes in the expression of COL1A1 . The transcript level of GAPDH was used as the loading control. No difference was found in the expression of COL1A1 between the patient and the control. C, Type I collagen secretion and deposition in fibroblasts from patient 10 and the control. Expression of COL1A1 was detected by an anti-COL1A1 antibody followed by a secondary antibody conjugated to Alexa Fluor 647, which appears in red. DAPI was used to stain nuclei. Scale bar =50 μm. The arrow indicates the exogenous type I collagen molecules in deposited matrix.
Figure Legend Snippet: Expression of β-catenin, WNT1, and collagen type I. A, Western blotting and semiquantitative analysis of β-catenin and WNT1 in bone samples from patient 10 and the control. Data were collected from 3 independent experiments. * P less than .05 vs the control. Statistical significance was assessed by a 2-tailed unpaired t test. B, Relative expression of COL1A1 in the fibroblasts from patient 10 and the control. Real-time quantitative polymerase chain reaction was performed to examine changes in the expression of COL1A1 . The transcript level of GAPDH was used as the loading control. No difference was found in the expression of COL1A1 between the patient and the control. C, Type I collagen secretion and deposition in fibroblasts from patient 10 and the control. Expression of COL1A1 was detected by an anti-COL1A1 antibody followed by a secondary antibody conjugated to Alexa Fluor 647, which appears in red. DAPI was used to stain nuclei. Scale bar =50 μm. The arrow indicates the exogenous type I collagen molecules in deposited matrix.

Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Staining


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Proteintech wnt1
Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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    Proteintech anti wnt1
    Anti Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti wnt1/product/Proteintech
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    86
    Proteintech wnt1
    Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Proteintech wnt inhibitory factor 1 wif1
    Wnt Inhibitory Factor 1 Wif1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti wnt 1
    Anti Wnt 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti wnt 1/product/Proteintech
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    Proteintech antibodies recognizing wnt1
    Effects of miR-34a on the <t>WNT1/β-catenin</t> axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.
    Antibodies Recognizing Wnt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies recognizing wnt1/product/Proteintech
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    Effects of miR-34a on the WNT1/β-catenin axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.

    Journal: Neural Regeneration Research

    Article Title: Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

    doi: 10.4103/1673-5374.367831

    Figure Lengend Snippet: Effects of miR-34a on the WNT1/β-catenin axis and SIRT1/Nrf2/HO-1 axis in hDPSCs. (A) WNT1, β-catenin, SIRT1, Nrf2, and HO-1 protein expression levels and their quantitative analysis ( n = 3). (B, C) Schematic of the predicted miR-34a binding site in wild type and mutant type of WNT1 (B) and SIRT1 mRNA (C). Luciferase reporter plasmids containing the wild-type (WT) or MUT 3'-UTR fragments were co-transfected with either miR-34a mimic or NC mimic. Red nucleotides indicate mutation sites. The intensity of luciferase activity was measured using luminescence ( n = 6). All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t -test). The lentiviral particles were miR-34a overexpression (miR-34a), lentiviral vector of miR-34a (miR-NC), miR-34a knockdown (anti-34a), and lentiviral vector of anti-34a (anti-NC). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.

    Article Snippet: The proteins were loaded into 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels (Servicebio) and then transferred onto polyvinylidene fluoride membranes (MilliporeSigma), which were then probed with primary antibodies recognizing WNT1 (1:1000; Proteintech, Wuhan, China, Cat#27935-1-AP, RRID: AB_2881013), β-catenin (1:1000; Proteintech, Cat# 51067-2-AP, RRID: AB_2086128), SIRT1 (1:1000; Proteintech, Cat#13161-1-AP, RRID: AB_10646436), Nrf2 (1:1000; Proteintech, Cat#16396-1-AP, RRID: AB_2782956), HO-1 (1:1000; Proteintech, Cat# 10701-1-AP, RRID: AB_2118685), Bcl-2 (1:1000; Proteintech, Cat# 26593-1-AP, RRID: AB_2818996), Bax (1:1000; Proteintech, Cat# 60267-1-Ig, RRID: AB_2848213), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Proteintech, Cat# 60004-1-Ig, RRID: AB_2107436).

    Techniques: Expressing, Binding Assay, Mutagenesis, Luciferase, Transfection, Activity Assay, Over Expression, Plasmid Preparation

    Effects of WNT1 or SIRT1 suppression on apoptosis in miR-34a knockdown cell line. (A) Protein expression of WNT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (B) Protein expression of SIRT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (magnification ×400; scale bar: 50 μm; DAPI: blue; TUNEL: red) and quantitative analysis of TUNEL staining ( n = 3). TUNEL staining was performed to assess hDPSC apoptosis following OGD/R for 24 hours. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t- test). DAPI: 4′,6-Diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells.

    Journal: Neural Regeneration Research

    Article Title: Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

    doi: 10.4103/1673-5374.367831

    Figure Lengend Snippet: Effects of WNT1 or SIRT1 suppression on apoptosis in miR-34a knockdown cell line. (A) Protein expression of WNT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (B) Protein expression of SIRT1 in hDPSCs after RNA interference and its quantitative analysis ( n = 3). (C) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining (magnification ×400; scale bar: 50 μm; DAPI: blue; TUNEL: red) and quantitative analysis of TUNEL staining ( n = 3). TUNEL staining was performed to assess hDPSC apoptosis following OGD/R for 24 hours. All data are presented as mean ± SD. * P < 0.05, ** P < 0.01 (Student’s t- test). DAPI: 4′,6-Diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; hDPSCs: human dental pulp stem cells.

    Article Snippet: The proteins were loaded into 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels (Servicebio) and then transferred onto polyvinylidene fluoride membranes (MilliporeSigma), which were then probed with primary antibodies recognizing WNT1 (1:1000; Proteintech, Wuhan, China, Cat#27935-1-AP, RRID: AB_2881013), β-catenin (1:1000; Proteintech, Cat# 51067-2-AP, RRID: AB_2086128), SIRT1 (1:1000; Proteintech, Cat#13161-1-AP, RRID: AB_10646436), Nrf2 (1:1000; Proteintech, Cat#16396-1-AP, RRID: AB_2782956), HO-1 (1:1000; Proteintech, Cat# 10701-1-AP, RRID: AB_2118685), Bcl-2 (1:1000; Proteintech, Cat# 26593-1-AP, RRID: AB_2818996), Bax (1:1000; Proteintech, Cat# 60267-1-Ig, RRID: AB_2848213), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Proteintech, Cat# 60004-1-Ig, RRID: AB_2107436).

    Techniques: Expressing, TUNEL Assay, Staining

    Effects of WNT1 or SIRT1 suppression on the proliferation and antioxidant capacity of miR-34a knockdown in hDPSCs. Superoxide dismutase (SOD) activity assay and dihydroethidium (DHE) staining were performed to assess the antioxidant capacity of hDPSCs following oxygen-glucose deprivation/reoxygenation for 24 hours. (A) 5-ethynyl-2′-deoxyuridine (EdU) staining (scale bar: 20 μm; DAPI: blue, EdU: red) and quantitative analysis of EdU staining ( n = 3). (B) Cell viability ( n = 6). (C) Percentage of SOD activity ( n = 6). (D) DHE staining (magnification ×200; scale bar: 20 μm; DHE: red) and quantitative analysis of DHE staining ( n = 3). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t -test). OD: Optical density.

    Journal: Neural Regeneration Research

    Article Title: Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke

    doi: 10.4103/1673-5374.367831

    Figure Lengend Snippet: Effects of WNT1 or SIRT1 suppression on the proliferation and antioxidant capacity of miR-34a knockdown in hDPSCs. Superoxide dismutase (SOD) activity assay and dihydroethidium (DHE) staining were performed to assess the antioxidant capacity of hDPSCs following oxygen-glucose deprivation/reoxygenation for 24 hours. (A) 5-ethynyl-2′-deoxyuridine (EdU) staining (scale bar: 20 μm; DAPI: blue, EdU: red) and quantitative analysis of EdU staining ( n = 3). (B) Cell viability ( n = 6). (C) Percentage of SOD activity ( n = 6). (D) DHE staining (magnification ×200; scale bar: 20 μm; DHE: red) and quantitative analysis of DHE staining ( n = 3). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t -test). OD: Optical density.

    Article Snippet: The proteins were loaded into 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels (Servicebio) and then transferred onto polyvinylidene fluoride membranes (MilliporeSigma), which were then probed with primary antibodies recognizing WNT1 (1:1000; Proteintech, Wuhan, China, Cat#27935-1-AP, RRID: AB_2881013), β-catenin (1:1000; Proteintech, Cat# 51067-2-AP, RRID: AB_2086128), SIRT1 (1:1000; Proteintech, Cat#13161-1-AP, RRID: AB_10646436), Nrf2 (1:1000; Proteintech, Cat#16396-1-AP, RRID: AB_2782956), HO-1 (1:1000; Proteintech, Cat# 10701-1-AP, RRID: AB_2118685), Bcl-2 (1:1000; Proteintech, Cat# 26593-1-AP, RRID: AB_2818996), Bax (1:1000; Proteintech, Cat# 60267-1-Ig, RRID: AB_2848213), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:1000; Proteintech, Cat# 60004-1-Ig, RRID: AB_2107436).

    Techniques: Activity Assay, Staining