wizard plus miniprep procedure  (Promega)

 
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    Name:
    Wizard Plus SV Minipreps DNA Purification Systems
    Description:
    Spin and vacuum based methods for plasmid purification from 1 10ml cultures
    Catalog Number:
    a1330
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Nucleic Acid Extraction Plasmid Purification
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    Structured Review

    Promega wizard plus miniprep procedure
    Spin and vacuum based methods for plasmid purification from 1 10ml cultures
    https://www.bioz.com/result/wizard plus miniprep procedure/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard plus miniprep procedure - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular Typing of Papillomatous Digital Dermatitis-Associated Treponema Isolates Based on Analysis of 16S-23S Ribosomal DNA Intergenic Spacer Regions
    Article Snippet: .. Recombinant plasmids from selected clones were purified with the Wizard Miniplus DNA purification system (Promega), and the inserts were confirmed by agarose gel electrophoresis following restriction enzyme digestion. .. The nucleotide sequences of the Treponema 16S-23S rDNA ISRs were determined with vector-based M13 primers at the University of North Carolina at Chapel Hill Automated DNA Sequencing Facility.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Typing of Papillomatous Digital Dermatitis-Associated Treponema Isolates Based on Analysis of 16S-23S Ribosomal DNA Intergenic Spacer Regions
    Article Snippet: .. Recombinant plasmids from selected clones were purified with the Wizard Miniplus DNA purification system (Promega), and the inserts were confirmed by agarose gel electrophoresis following restriction enzyme digestion. .. The nucleotide sequences of the Treponema 16S-23S rDNA ISRs were determined with vector-based M13 primers at the University of North Carolina at Chapel Hill Automated DNA Sequencing Facility.

    DNA Purification:

    Article Title: Extracellular Synthesis, Specific Recognition, and Intracellular Degradation of Cyclomaltodextrins by the Hyperthermophilic Archaeon Thermococcus sp. Strain B1001
    Article Snippet: .. Plasmids for DNA sequencing were extracted and purified by using a Wizard Plus Minipreps DNA Purification System (Promega, Madison, Wis.). ..

    Article Title: The identification of markers segregating with resistance toSchistosoma mansoni infection in the snailBiomphalaria glabrata
    Article Snippet: .. Recombinant plasmid DNA was isolated with the Wizard miniprep DNA purification system (Promega) and sequenced by the dideoxy-sequencing method with the T7 Sequenase kit (Amersham) with universal primers M13 and synthetic commercial primers (Genosys, The Woodlands, TX) to complete the sequencing of both strands of the template. ..

    Article Title: Surprising Dependence on Postsegregational Killing of Host Cells for Maintenance of the Large Virulence Plasmid of Shigella flexneri
    Article Snippet: .. Plasmid DNA was prepared using the Wizard Plus Miniprep/Maxiprep DNA purification system (Promega Corp., Madison, Wis.). .. E. coli strain DH5α was used for plasmid growth and DNA manipulations.

    Article Title: Maternally Inherited Partial Monosomy 9p (pter - > p24.1) and Partial Trisomy 20p (pter - > p12.1) Characterized by Microarray Comparative Genomic Hybridization
    Article Snippet: .. BAC DNAs were extracted using Wizard® Plus SV Minipreps DNA Purification System (Promega Corporation, Madison, WI) and labeled by Nick Translation Mix (Roche Applied Science, Indianapolis, IN) with Fluorescein-12-dUTP or Tetramethil-rhodamine-6-dUTP. ..

    Article Title: Molecular Typing of Papillomatous Digital Dermatitis-Associated Treponema Isolates Based on Analysis of 16S-23S Ribosomal DNA Intergenic Spacer Regions
    Article Snippet: .. Recombinant plasmids from selected clones were purified with the Wizard Miniplus DNA purification system (Promega), and the inserts were confirmed by agarose gel electrophoresis following restriction enzyme digestion. .. The nucleotide sequences of the Treponema 16S-23S rDNA ISRs were determined with vector-based M13 primers at the University of North Carolina at Chapel Hill Automated DNA Sequencing Facility.

    Isolation:

    Article Title: The identification of markers segregating with resistance toSchistosoma mansoni infection in the snailBiomphalaria glabrata
    Article Snippet: .. Recombinant plasmid DNA was isolated with the Wizard miniprep DNA purification system (Promega) and sequenced by the dideoxy-sequencing method with the T7 Sequenase kit (Amersham) with universal primers M13 and synthetic commercial primers (Genosys, The Woodlands, TX) to complete the sequencing of both strands of the template. ..

    Article Title: Transcriptional Regulation of the virR Operon of the Intracellular Pathogen Rhodococcus equi ▿
    Article Snippet: .. Plasmid DNA was isolated via the alkaline lysis method of Birnboim and Doly ( ) or by using a Wizard Plus SV miniprep kit as described by the manufacturer (Promega). .. DNA fragments were isolated from agarose gels by using a Genelute DNA purification kit as described by the manufacturer (Sigma-Aldrich).

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling
    Article Snippet: .. Plasmid DNA was isolated by using either the Wizard Plus SV Minipreps purification system (Promega) according to the manufacturers protocol or the XSP buffer extraction method described by De Ungria et al. ( ). ..

    Nick Translation:

    Article Title: Maternally Inherited Partial Monosomy 9p (pter - > p24.1) and Partial Trisomy 20p (pter - > p12.1) Characterized by Microarray Comparative Genomic Hybridization
    Article Snippet: .. BAC DNAs were extracted using Wizard® Plus SV Minipreps DNA Purification System (Promega Corporation, Madison, WI) and labeled by Nick Translation Mix (Roche Applied Science, Indianapolis, IN) with Fluorescein-12-dUTP or Tetramethil-rhodamine-6-dUTP. ..

    Labeling:

    Article Title: Maternally Inherited Partial Monosomy 9p (pter - > p24.1) and Partial Trisomy 20p (pter - > p12.1) Characterized by Microarray Comparative Genomic Hybridization
    Article Snippet: .. BAC DNAs were extracted using Wizard® Plus SV Minipreps DNA Purification System (Promega Corporation, Madison, WI) and labeled by Nick Translation Mix (Roche Applied Science, Indianapolis, IN) with Fluorescein-12-dUTP or Tetramethil-rhodamine-6-dUTP. ..

    Purification:

    Article Title: Extracellular Synthesis, Specific Recognition, and Intracellular Degradation of Cyclomaltodextrins by the Hyperthermophilic Archaeon Thermococcus sp. Strain B1001
    Article Snippet: .. Plasmids for DNA sequencing were extracted and purified by using a Wizard Plus Minipreps DNA Purification System (Promega, Madison, Wis.). ..

    Article Title: Molecular Typing of Papillomatous Digital Dermatitis-Associated Treponema Isolates Based on Analysis of 16S-23S Ribosomal DNA Intergenic Spacer Regions
    Article Snippet: .. Recombinant plasmids from selected clones were purified with the Wizard Miniplus DNA purification system (Promega), and the inserts were confirmed by agarose gel electrophoresis following restriction enzyme digestion. .. The nucleotide sequences of the Treponema 16S-23S rDNA ISRs were determined with vector-based M13 primers at the University of North Carolina at Chapel Hill Automated DNA Sequencing Facility.

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling
    Article Snippet: .. Plasmid DNA was isolated by using either the Wizard Plus SV Minipreps purification system (Promega) according to the manufacturers protocol or the XSP buffer extraction method described by De Ungria et al. ( ). ..

    Produced:

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling
    Article Snippet: .. The xanthogenate-SDS-phenol method of plasmid extraction ( ) produced better-quality plasmids and less contamination with chromosomal DNA than the Promega Wizard Plus SV Minipreps method (Fig. ), which was employed in earlier studies ( , ). .. Plasmid pHel6, isolated from strain P12, was difficult to isolate by either method, probably due to a high DNase activity in this strain (data not shown).

    Alkaline Lysis:

    Article Title: Transcriptional Regulation of the virR Operon of the Intracellular Pathogen Rhodococcus equi ▿
    Article Snippet: .. Plasmid DNA was isolated via the alkaline lysis method of Birnboim and Doly ( ) or by using a Wizard Plus SV miniprep kit as described by the manufacturer (Promega). .. DNA fragments were isolated from agarose gels by using a Genelute DNA purification kit as described by the manufacturer (Sigma-Aldrich).

    DNA Sequencing:

    Article Title: Extracellular Synthesis, Specific Recognition, and Intracellular Degradation of Cyclomaltodextrins by the Hyperthermophilic Archaeon Thermococcus sp. Strain B1001
    Article Snippet: .. Plasmids for DNA sequencing were extracted and purified by using a Wizard Plus Minipreps DNA Purification System (Promega, Madison, Wis.). ..

    BAC Assay:

    Article Title: Maternally Inherited Partial Monosomy 9p (pter - > p24.1) and Partial Trisomy 20p (pter - > p12.1) Characterized by Microarray Comparative Genomic Hybridization
    Article Snippet: .. BAC DNAs were extracted using Wizard® Plus SV Minipreps DNA Purification System (Promega Corporation, Madison, WI) and labeled by Nick Translation Mix (Roche Applied Science, Indianapolis, IN) with Fluorescein-12-dUTP or Tetramethil-rhodamine-6-dUTP. ..

    Sequencing:

    Article Title: The identification of markers segregating with resistance toSchistosoma mansoni infection in the snailBiomphalaria glabrata
    Article Snippet: .. Recombinant plasmid DNA was isolated with the Wizard miniprep DNA purification system (Promega) and sequenced by the dideoxy-sequencing method with the T7 Sequenase kit (Amersham) with universal primers M13 and synthetic commercial primers (Genosys, The Woodlands, TX) to complete the sequencing of both strands of the template. ..

    Recombinant:

    Article Title: The identification of markers segregating with resistance toSchistosoma mansoni infection in the snailBiomphalaria glabrata
    Article Snippet: .. Recombinant plasmid DNA was isolated with the Wizard miniprep DNA purification system (Promega) and sequenced by the dideoxy-sequencing method with the T7 Sequenase kit (Amersham) with universal primers M13 and synthetic commercial primers (Genosys, The Woodlands, TX) to complete the sequencing of both strands of the template. ..

    Article Title: Molecular Typing of Papillomatous Digital Dermatitis-Associated Treponema Isolates Based on Analysis of 16S-23S Ribosomal DNA Intergenic Spacer Regions
    Article Snippet: .. Recombinant plasmids from selected clones were purified with the Wizard Miniplus DNA purification system (Promega), and the inserts were confirmed by agarose gel electrophoresis following restriction enzyme digestion. .. The nucleotide sequences of the Treponema 16S-23S rDNA ISRs were determined with vector-based M13 primers at the University of North Carolina at Chapel Hill Automated DNA Sequencing Facility.

    Plasmid Preparation:

    Article Title: The identification of markers segregating with resistance toSchistosoma mansoni infection in the snailBiomphalaria glabrata
    Article Snippet: .. Recombinant plasmid DNA was isolated with the Wizard miniprep DNA purification system (Promega) and sequenced by the dideoxy-sequencing method with the T7 Sequenase kit (Amersham) with universal primers M13 and synthetic commercial primers (Genosys, The Woodlands, TX) to complete the sequencing of both strands of the template. ..

    Article Title: Transcriptional Regulation of the virR Operon of the Intracellular Pathogen Rhodococcus equi ▿
    Article Snippet: .. Plasmid DNA was isolated via the alkaline lysis method of Birnboim and Doly ( ) or by using a Wizard Plus SV miniprep kit as described by the manufacturer (Promega). .. DNA fragments were isolated from agarose gels by using a Genelute DNA purification kit as described by the manufacturer (Sigma-Aldrich).

    Article Title: Surprising Dependence on Postsegregational Killing of Host Cells for Maintenance of the Large Virulence Plasmid of Shigella flexneri
    Article Snippet: .. Plasmid DNA was prepared using the Wizard Plus Miniprep/Maxiprep DNA purification system (Promega Corp., Madison, Wis.). .. E. coli strain DH5α was used for plasmid growth and DNA manipulations.

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling
    Article Snippet: .. Plasmid DNA was isolated by using either the Wizard Plus SV Minipreps purification system (Promega) according to the manufacturers protocol or the XSP buffer extraction method described by De Ungria et al. ( ). ..

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling
    Article Snippet: .. The xanthogenate-SDS-phenol method of plasmid extraction ( ) produced better-quality plasmids and less contamination with chromosomal DNA than the Promega Wizard Plus SV Minipreps method (Fig. ), which was employed in earlier studies ( , ). .. Plasmid pHel6, isolated from strain P12, was difficult to isolate by either method, probably due to a high DNase activity in this strain (data not shown).

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  • 99
    Promega wizard plus sv minipreps method
    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV <t>Minipreps</t> (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.
    Wizard Plus Sv Minipreps Method, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard plus sv minipreps method/product/Promega
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    wizard plus sv minipreps method - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Journal: Journal of Bacteriology

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling

    doi: 10.1128/JB.184.10.2755-2766.2002

    Figure Lengend Snippet: Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Article Snippet: The xanthogenate-SDS-phenol method of plasmid extraction ( ) produced better-quality plasmids and less contamination with chromosomal DNA than the Promega Wizard Plus SV Minipreps method (Fig. ), which was employed in earlier studies ( , ).

    Techniques: Purification, Isolation, Agarose Gel Electrophoresis, Marker