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Promega wizard plus miniprep procedure
Wizard Plus Miniprep Procedure, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard plus miniprep procedure/product/Promega
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wizard plus miniprep procedure - by Bioz Stars, 2020-01
79/100 stars

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Clone Assay:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Amplification:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: Paragraph title: Specific PCR amplification. ... The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega).

Agarose Gel Electrophoresis:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega).

Isolation:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Purification:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega).

Polymerase Chain Reaction:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: Paragraph title: Specific PCR amplification. ... The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega).

Plasmid Preparation:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Sequencing:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Transformation Assay:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Recombinant:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was isolated from a 1.5% SeaKem GTG agarose gel (FMC BioProducts, Rockland, Maine) and was purified by use of a GenElute agarose spin column (Supelco, Bellafonte, Pa.). .. The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. Inserts were sequenced using pUC/M13 forward and reverse primers and the Big Dye terminator cycle sequencing ready reaction kit (PE Applied Biosystems, Stockholm, Sweden).

Derivative Assay:

Article Title: Evaluation of PCR-Based Methods for Discrimination of Francisella Species and Subspecies and Development of a Specific PCR That Distinguishes the Two Major Subspecies of Francisella tularensis
Article Snippet: The fragment was ligated into vector pGEM-T according to the instructions of the manufacturer (Promega Corp., Madison, Wis.) After transformation of Escherichia coli DH5α, recombinant clones were identified by blue-white color screening; plasmid DNA was isolated by the Wizard Plus miniprep procedure (Promega). .. The sequence was analyzed using an ABI 377 sequencer (PE Applied Biosystems).

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  • 79
    Promega wizard plus miniprep procedure
    Wizard Plus Miniprep Procedure, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard plus miniprep procedure/product/Promega
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard plus miniprep procedure - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    77
    Promega wizard plus sv minipreps method
    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV <t>Minipreps</t> (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.
    Wizard Plus Sv Minipreps Method, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard plus sv minipreps method/product/Promega
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard plus sv minipreps method - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    79
    Promega alkaline mini prep method
    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV <t>Minipreps</t> (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.
    Alkaline Mini Prep Method, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline mini prep method/product/Promega
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alkaline mini prep method - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

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    Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Journal:

    Article Title: Characterization of Two Cryptic Helicobacter pylori Plasmids: a Putative Source for Horizontal Gene Transfer and Gene Shuffling

    doi: 10.1128/JB.184.10.2755-2766.2002

    Figure Lengend Snippet: Purification and analysis of H. pylori plasmids by restriction enzyme digestion. Plasmids pHel4 and pHel5 were isolated from H. pylori strains P8 and P29, respectively, by the Wizard Plus SV Minipreps (Promega) and the xanthogenate-SDS-phenol methods. Plasmids were digested with restriction endonucleases Bgl II (lane 1), Eco RI (lane 2), Hin dIII (lane 3), and Sac I (lane 4) and separated on a 0.8% agarose gel. ∗, partially digested Eco RI fragment. Lane M, marker. kb, kilobases.

    Article Snippet: The xanthogenate-SDS-phenol method of plasmid extraction ( ) produced better-quality plasmids and less contamination with chromosomal DNA than the Promega Wizard Plus SV Minipreps method (Fig. ), which was employed in earlier studies ( , ).

    Techniques: Purification, Isolation, Agarose Gel Electrophoresis, Marker