wizard pcr clean up system  (Promega)

 
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    Name:
    Wizard DNA Clean Up System
    Description:
    Purifies linear and circular DNA 200 50 000bp from many molecular biology reactions
    Catalog Number:
    a7280
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Nucleic Acid Extraction Clean up and Concentration
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    Structured Review

    Promega wizard pcr clean up system
    <t>PCR</t> and <t>DNA</t> sequencing
    Purifies linear and circular DNA 200 50 000bp from many molecular biology reactions
    https://www.bioz.com/result/wizard pcr clean up system/product/Promega
    Average 96 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    wizard pcr clean up system - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Production of a unique pneumococcal capsule serotype belonging to serogroup 6"

    Article Title: Production of a unique pneumococcal capsule serotype belonging to serogroup 6

    Journal:

    doi: 10.1099/mic.0.024521-0

    PCR and DNA sequencing
    Figure Legend Snippet: PCR and DNA sequencing

    Techniques Used: Polymerase Chain Reaction, DNA Sequencing

    2) Product Images from "An Invariant T Cell Receptor ? Chain Defines a Novel TAP-independent Major Histocompatibility Complex Class Ib-restricted ?/? T Cell Subpopulation in Mammals "

    Article Title: An Invariant T Cell Receptor ? Chain Defines a Novel TAP-independent Major Histocompatibility Complex Class Ib-restricted ?/? T Cell Subpopulation in Mammals

    Journal: The Journal of Experimental Medicine

    doi:

    Direct enumeration of AV7S2/AV19-AJ33–bearing cells by PCR–limiting dilution analysis and single cell PCR. (A) DN cells obtained from three human subjects were seeded into microtiter plates using a FACS ® single cell deposition unit. After cell lysis, PCR was carried out with AV7S2 and AJ33 primers, and the amount of amplicons was quantified by ELISA. (B and C) α/β CD8α + or CD4 + cells were obtained by FACS ® sorting, serially diluted, distributed in a microtiter plate at the indicated cell concentration, and processed as above. In C (CD4 + cells), the amplicons of the six wells indicated (which had a high probability of clonality) were sequenced. The sequences were as follows:1-GCT GTG ATG TCG AT2-GCT GTG AGA GAG ATC GGA3- GCT GTG AGA GAT 4-GCT GNN ACT CGA5-GCT GTG AGA GAG GAT AAC-AJ34-intron-AJ336-GCT GTG AGG GGG GGG GAA AAC-AJ34-intron-AJ33Only one (sequence 3) corresponds to the canonical AV7S2-AJ33 rearrangement (see text for details). In B and C, frequencies were calculated by maximum likelihood analysis.
    Figure Legend Snippet: Direct enumeration of AV7S2/AV19-AJ33–bearing cells by PCR–limiting dilution analysis and single cell PCR. (A) DN cells obtained from three human subjects were seeded into microtiter plates using a FACS ® single cell deposition unit. After cell lysis, PCR was carried out with AV7S2 and AJ33 primers, and the amount of amplicons was quantified by ELISA. (B and C) α/β CD8α + or CD4 + cells were obtained by FACS ® sorting, serially diluted, distributed in a microtiter plate at the indicated cell concentration, and processed as above. In C (CD4 + cells), the amplicons of the six wells indicated (which had a high probability of clonality) were sequenced. The sequences were as follows:1-GCT GTG ATG TCG AT2-GCT GTG AGA GAG ATC GGA3- GCT GTG AGA GAT 4-GCT GNN ACT CGA5-GCT GTG AGA GAG GAT AAC-AJ34-intron-AJ336-GCT GTG AGG GGG GGG GAA AAC-AJ34-intron-AJ33Only one (sequence 3) corresponds to the canonical AV7S2-AJ33 rearrangement (see text for details). In B and C, frequencies were calculated by maximum likelihood analysis.

    Techniques Used: Polymerase Chain Reaction, FACS, Lysis, Enzyme-linked Immunosorbent Assay, Concentration Assay, Activated Clotting Time Assay, Sequencing

    Related Articles

    Amplification:

    Article Title: Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography
    Article Snippet: .. One microgram of genomic DNA was treated with sodium bisulfite as described ( ) and the Wizard DNA Clean-Up System Kit (A7280; Promega), subsequently used prior to PCR amplification. .. During this modification unmethylated C residues are converted to U (T in PCR products), while methylated C residues are not.

    Agarose Gel Electrophoresis:

    Article Title: Eubacterial SpoVG Homologs Constitute a New Family of Site-Specific DNA-Binding Proteins
    Article Snippet: .. Amplicons were separated by agarose gel electrophoresis and purified using Wizard DNA Clean-up Systems (Promega, Madison, WI) before use as EMSA competitors. ..

    Purification:

    Article Title: Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island
    Article Snippet: .. PCR amplicons purified using the Wizard PCR DNA Cleanup system (Promega Corporation, Madison, Wis.) were sequenced using an Applied Biosystems Prism 377 automated DNA sequencer using the Taq dye-deoxy terminator method (PE Applied Biosystems, Foster City, Calif.). ..

    Article Title: Eubacterial SpoVG Homologs Constitute a New Family of Site-Specific DNA-Binding Proteins
    Article Snippet: .. Amplicons were separated by agarose gel electrophoresis and purified using Wizard DNA Clean-up Systems (Promega, Madison, WI) before use as EMSA competitors. ..

    Article Title: Prophage Carriage as a Molecular Epidemiological Marker in Streptococcus pneumoniae
    Article Snippet: .. The 1.2-kb fragment containing the lytA gene was purified from the gel by using the Wizard DNA Cleanup kit. .. A second DNA probe was generated by PCR to include an 890-nt internal fragment of lytA from which the first 57 and last 10 nt of the lytA gene were missing.

    Article Title: DNA methylation profiling of ovarian carcinomas and their in vitro models identifies HOXA9, HOXB5, SCGB3A1, and CRABP1 as novel targets
    Article Snippet: .. Hydroquinone (Sigma Chemical Co., St. Louis, MO, USA) and sodium bisulphite (Sigma Chemical Co, USA) at pH 5.0 were added to the samples to a final concentration of one mM and 3.7 M, respectively, prior to incubation at 50°C for 16 h. Bisulphite treated DNA was purified using the Wizard DNA clean-up kit (Promega Corp, Madison, WI, USA) and eluted in 100 μL MQ water. .. In order to complete the conversion of unmethylated cytosines, NaOH was added to a final concentration of 0.3 M and the samples were incubated for 15 minutes at 37°C.

    Article Title: The Effects of Promoter Methylation on Downregulation of DAZAP2 in Multiple Myeloma Cell Lines
    Article Snippet: .. Plasmids purification mini kit, DNA Wizard clean-up system, the pGL2 Luciferase Reporter Vectors, Luciferase Assay System, β-galactosidase immuosorbent were from Promega. .. Lipofectamine™ LTX regent was from Invitrogen.

    Concentration Assay:

    Article Title: DNA methylation profiling of ovarian carcinomas and their in vitro models identifies HOXA9, HOXB5, SCGB3A1, and CRABP1 as novel targets
    Article Snippet: .. Hydroquinone (Sigma Chemical Co., St. Louis, MO, USA) and sodium bisulphite (Sigma Chemical Co, USA) at pH 5.0 were added to the samples to a final concentration of one mM and 3.7 M, respectively, prior to incubation at 50°C for 16 h. Bisulphite treated DNA was purified using the Wizard DNA clean-up kit (Promega Corp, Madison, WI, USA) and eluted in 100 μL MQ water. .. In order to complete the conversion of unmethylated cytosines, NaOH was added to a final concentration of 0.3 M and the samples were incubated for 15 minutes at 37°C.

    Incubation:

    Article Title: DNA methylation profiling of ovarian carcinomas and their in vitro models identifies HOXA9, HOXB5, SCGB3A1, and CRABP1 as novel targets
    Article Snippet: .. Hydroquinone (Sigma Chemical Co., St. Louis, MO, USA) and sodium bisulphite (Sigma Chemical Co, USA) at pH 5.0 were added to the samples to a final concentration of one mM and 3.7 M, respectively, prior to incubation at 50°C for 16 h. Bisulphite treated DNA was purified using the Wizard DNA clean-up kit (Promega Corp, Madison, WI, USA) and eluted in 100 μL MQ water. .. In order to complete the conversion of unmethylated cytosines, NaOH was added to a final concentration of 0.3 M and the samples were incubated for 15 minutes at 37°C.

    Luciferase:

    Article Title: The Effects of Promoter Methylation on Downregulation of DAZAP2 in Multiple Myeloma Cell Lines
    Article Snippet: .. Plasmids purification mini kit, DNA Wizard clean-up system, the pGL2 Luciferase Reporter Vectors, Luciferase Assay System, β-galactosidase immuosorbent were from Promega. .. Lipofectamine™ LTX regent was from Invitrogen.

    DNA Purification:

    Article Title: Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments
    Article Snippet: .. Moreover, no PCR products were obtained after additional extracellular DNA purification with the Wizard DNA Clean-up system (Promega) or by conventional cesium chloride density gradient centrifugation. .. Dot blot analyses carried out with a digoxigenin-labeled probe (prepared by PCR amplification of the 16S rRNA gene of E. coli ) indicated a lack of hybridization with extracellular DNA recovered from all sediment samples (data not shown).

    Polymerase Chain Reaction:

    Article Title: Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments
    Article Snippet: .. Moreover, no PCR products were obtained after additional extracellular DNA purification with the Wizard DNA Clean-up system (Promega) or by conventional cesium chloride density gradient centrifugation. .. Dot blot analyses carried out with a digoxigenin-labeled probe (prepared by PCR amplification of the 16S rRNA gene of E. coli ) indicated a lack of hybridization with extracellular DNA recovered from all sediment samples (data not shown).

    Article Title: Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island
    Article Snippet: .. PCR amplicons purified using the Wizard PCR DNA Cleanup system (Promega Corporation, Madison, Wis.) were sequenced using an Applied Biosystems Prism 377 automated DNA sequencer using the Taq dye-deoxy terminator method (PE Applied Biosystems, Foster City, Calif.). ..

    Article Title: Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography
    Article Snippet: .. One microgram of genomic DNA was treated with sodium bisulfite as described ( ) and the Wizard DNA Clean-Up System Kit (A7280; Promega), subsequently used prior to PCR amplification. .. During this modification unmethylated C residues are converted to U (T in PCR products), while methylated C residues are not.

    Gradient Centrifugation:

    Article Title: Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments
    Article Snippet: .. Moreover, no PCR products were obtained after additional extracellular DNA purification with the Wizard DNA Clean-up system (Promega) or by conventional cesium chloride density gradient centrifugation. .. Dot blot analyses carried out with a digoxigenin-labeled probe (prepared by PCR amplification of the 16S rRNA gene of E. coli ) indicated a lack of hybridization with extracellular DNA recovered from all sediment samples (data not shown).

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  • 99
    Promega dna wizard sv gel
    Southern blot of apospory-specific <t>AFLP-derived</t> sequences in <t>DNA</t> from sexual and apomictic Paspalum notatum. A, B and C: Hybridization of DNA from genotypes Q4188 (SP) and Q4117 (AP) with clones PnMAM3 , PnMAC5 and PnMAI3 , respectively, after digestion with three restriction enzymes. D: Hybridization of PnMAI3 against sexual (SB) and apomictic (AB) bulks obtained from ten F 1 sexual and apomictic progenies, respectively. Arrows indicate polymorphic bands between parental plants and sexual and apomictic bulks.
    Dna Wizard Sv Gel, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna wizard sv gel/product/Promega
    Average 99 stars, based on 3070 article reviews
    Price from $9.99 to $1999.99
    dna wizard sv gel - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Southern blot of apospory-specific AFLP-derived sequences in DNA from sexual and apomictic Paspalum notatum. A, B and C: Hybridization of DNA from genotypes Q4188 (SP) and Q4117 (AP) with clones PnMAM3 , PnMAC5 and PnMAI3 , respectively, after digestion with three restriction enzymes. D: Hybridization of PnMAI3 against sexual (SB) and apomictic (AB) bulks obtained from ten F 1 sexual and apomictic progenies, respectively. Arrows indicate polymorphic bands between parental plants and sexual and apomictic bulks.

    Journal: Genetics and Molecular Biology

    Article Title: Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

    doi: 10.1590/S1415-47572012005000070

    Figure Lengend Snippet: Southern blot of apospory-specific AFLP-derived sequences in DNA from sexual and apomictic Paspalum notatum. A, B and C: Hybridization of DNA from genotypes Q4188 (SP) and Q4117 (AP) with clones PnMAM3 , PnMAC5 and PnMAI3 , respectively, after digestion with three restriction enzymes. D: Hybridization of PnMAI3 against sexual (SB) and apomictic (AB) bulks obtained from ten F 1 sexual and apomictic progenies, respectively. Arrows indicate polymorphic bands between parental plants and sexual and apomictic bulks.

    Article Snippet: DNA fragments were precipitated with absolute ethanol, dried at room temperature, dissolved in 20 μL of distilled water, re-amplified using the corresponding RAPD or AFLP primers and purified with the DNA Wizard SV Gel and PCR Clean-up system (Promega).

    Techniques: Southern Blot, Derivative Assay, Hybridization, Clone Assay

    Identification of osKaR insertion site Arbitrary-primed PCR (AP-PCR) is a quick method to precisely identify the genomic region where a transposon has inserted. The following is specific for osKaR insertions: genomic DNA of the osKaR mutant is extracted (see Basic Protocol 1) and used for a semi-random PCR using the osKaR -specific primer oPCR1 and the random primer Deg3. The Deg3 primer consists of an 11-nucleotide random primer (in blue) with a 25-nucleotide specific tail (in red). The resulting PCR product is purified and used for a 2 nd PCR using the osKaR -specific primer Anchor1 and the Deg3-tail specific primer Deg4. The resulting PCR product is purified and DNA sequencing is performed using the osKaR -specific primer Anchor2.

    Journal: Current protocols in microbiology

    Article Title: Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)

    doi: 10.1002/9780471729259.mc09d03s30

    Figure Lengend Snippet: Identification of osKaR insertion site Arbitrary-primed PCR (AP-PCR) is a quick method to precisely identify the genomic region where a transposon has inserted. The following is specific for osKaR insertions: genomic DNA of the osKaR mutant is extracted (see Basic Protocol 1) and used for a semi-random PCR using the osKaR -specific primer oPCR1 and the random primer Deg3. The Deg3 primer consists of an 11-nucleotide random primer (in blue) with a 25-nucleotide specific tail (in red). The resulting PCR product is purified and used for a 2 nd PCR using the osKaR -specific primer Anchor1 and the Deg3-tail specific primer Deg4. The resulting PCR product is purified and DNA sequencing is performed using the osKaR -specific primer Anchor2.

    Article Snippet: Genomic DNA of GAS osKaR mutant (see Basic Protocol 1) Primers oPCR1, Deg3, Anchor1, Deg4 and Anchor2 (see recipe) Reagents and equipment for PCR Gel and PCR Clean-Up System kit (Wizard SV, Promega Cat. No. A9282)

    Techniques: Polymerase Chain Reaction, Mutagenesis, Purification, DNA Sequencing

    IGF2BP1 promotes SRF expression in a 3′UTR and m 6 A-dependent manner. ( A ) Schematic depicting the position of IGF2BP1-CLIP sites reported in the SRF 3′UTR by six experiments performed in three indicated cell lines. ( B ) Schematic showing the SRF-3′UTR deletion strategy by CRISPR/Cas9. The relative position of sgRNAs and PCR primers for validating deletion of the SRF 3′UTR are indicated. ( C ) Representative semi-quantitative PCR analysis (left panel) of parental (WT) and SRF-3′UTR-deleted (Δ3′UTR) A549 cells. The successful deletion was further validated by DNA sequencing (right panel) of PCR products (Δ3′UTR) spanning the expected cleavage sites indicated in the schematic. ( D ) RT-qPCR analysis of indicated mRNAs in parental and SRF-Δ3′UTR A549 cells upon IGF2BP1 depletion (72 h). RPLP0 served as the normalization and GAPDH as the negative control. ( E ) Representative western blot analysis of indicated proteins in cells treated as described in (D). GAPDH served as the loading and normalization control for the quantification ( n = 3) of SRF protein levels upon IGF2BP1 depletion (relative to controls), as depicted above lower panel. ( F ) m 6 A -RIP-seq data showing m 6 ). ( G ) Representative western blot analysis of indicated proteins upon METTL3/14 depletion in parental (WT) and SRF-Δ3′UTR A549 cells. Note that IGF2BP1 expression is unaffected by METTL3/14 depletion, whereas SRF protein abundance is decreased only in parental A549 cells. GAPDH served as the loading and normalization control for the quantification ( n = 3) of SRF protein levels (relative to controls), as indicated in the lower panel. ( H ) The depletion of METTL3/14 by siRNA pools impairs SRF mRNA abundance in indicated cell lines. GAPDH served as the negative control in RT-qPCR studies cross-normalized to RPLP0 expression. ( I ) Altered m 6 A-modification of the SRF 3′UTR was determined upon METTL14 depletion in HepG2 cells by m 6 ). Note that the m 6 A-modified region partially overlaps with IGF2BP1-CLIP sites determined in HepG2 cells. ( J ) IGF2BP1-RIP analyses showing reduced association of the SRF mRNA with IGF2BP1 in METTL3/14-depleted A549 cells. RNA co-purified with IGF2BP1 from cells transfected with control siRNAs (siC) or METTL3/14-depleted cells was analyzed by RT-qPCR. HIST1H2AC served as the normalization and HIST2H3A as the negative control. Error bars indicate standard deviation determined in at least three analyses. Statistical significance was determined by Student’s t -test: (**) P

    Journal: Nucleic Acids Research

    Article Title: IGF2BP1 promotes SRF-dependent transcription in cancer in a m6A- and miRNA-dependent manner

    doi: 10.1093/nar/gky1012

    Figure Lengend Snippet: IGF2BP1 promotes SRF expression in a 3′UTR and m 6 A-dependent manner. ( A ) Schematic depicting the position of IGF2BP1-CLIP sites reported in the SRF 3′UTR by six experiments performed in three indicated cell lines. ( B ) Schematic showing the SRF-3′UTR deletion strategy by CRISPR/Cas9. The relative position of sgRNAs and PCR primers for validating deletion of the SRF 3′UTR are indicated. ( C ) Representative semi-quantitative PCR analysis (left panel) of parental (WT) and SRF-3′UTR-deleted (Δ3′UTR) A549 cells. The successful deletion was further validated by DNA sequencing (right panel) of PCR products (Δ3′UTR) spanning the expected cleavage sites indicated in the schematic. ( D ) RT-qPCR analysis of indicated mRNAs in parental and SRF-Δ3′UTR A549 cells upon IGF2BP1 depletion (72 h). RPLP0 served as the normalization and GAPDH as the negative control. ( E ) Representative western blot analysis of indicated proteins in cells treated as described in (D). GAPDH served as the loading and normalization control for the quantification ( n = 3) of SRF protein levels upon IGF2BP1 depletion (relative to controls), as depicted above lower panel. ( F ) m 6 A -RIP-seq data showing m 6 ). ( G ) Representative western blot analysis of indicated proteins upon METTL3/14 depletion in parental (WT) and SRF-Δ3′UTR A549 cells. Note that IGF2BP1 expression is unaffected by METTL3/14 depletion, whereas SRF protein abundance is decreased only in parental A549 cells. GAPDH served as the loading and normalization control for the quantification ( n = 3) of SRF protein levels (relative to controls), as indicated in the lower panel. ( H ) The depletion of METTL3/14 by siRNA pools impairs SRF mRNA abundance in indicated cell lines. GAPDH served as the negative control in RT-qPCR studies cross-normalized to RPLP0 expression. ( I ) Altered m 6 A-modification of the SRF 3′UTR was determined upon METTL14 depletion in HepG2 cells by m 6 ). Note that the m 6 A-modified region partially overlaps with IGF2BP1-CLIP sites determined in HepG2 cells. ( J ) IGF2BP1-RIP analyses showing reduced association of the SRF mRNA with IGF2BP1 in METTL3/14-depleted A549 cells. RNA co-purified with IGF2BP1 from cells transfected with control siRNAs (siC) or METTL3/14-depleted cells was analyzed by RT-qPCR. HIST1H2AC served as the normalization and HIST2H3A as the negative control. Error bars indicate standard deviation determined in at least three analyses. Statistical significance was determined by Student’s t -test: (**) P

    Article Snippet: DNA was finally eluted using the WIZARD® SV Gel & PCR Clean-Up System (Promega A9281) according to the manufacturer’s protocol and analyzed by quantitative real-time PCR (qPCR).

    Techniques: Expressing, Cross-linking Immunoprecipitation, CRISPR, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, DNA Sequencing, Quantitative RT-PCR, Negative Control, Western Blot, Modification, Purification, Transfection, Standard Deviation

    Establishment of conditional Ssu72-knockout DT40 cell lines. (A) Schematic representations of the chicken Ssu72 genomic fragment, knockout constructs, and configuration of the targeted alleles. Exons are shown as black boxes (E1–5), and the location of the 5′ probe used for Southern blotting is shown as a grey box. The double-headed arrows above the genes indicate length (in nucleotides). The XbaI and EcoRI restriction sites are indicated by vertical lines labeled X and R, respectively. (B) Southern blot analysis of wild-type (WT), heterozygous mutant (B15), homozygous mutant (P1, P2, P3), and unanticipated rearranged mutant (P10) clones. Genomic DNA obtained from each clone was digested with Xba I and Eco RI, and then hybridized with the 5′ probe shown in panel A. (C) RT-PCR analysis of the wild-type and mutant clones using primer pairs specific for the indicated gene. (D) Immunoblotting analysis of DT40 P3 (−/−) whole-cell extracts treated with Dox for the indicated times, using the indicated antibodies. Western blotting of β-actin was used as to confirm equal protein loading.

    Journal: PLoS ONE

    Article Title: Vertebrate Ssu72 Regulates and Coordinates 3′-End Formation of RNAs Transcribed by RNA Polymerase II

    doi: 10.1371/journal.pone.0106040

    Figure Lengend Snippet: Establishment of conditional Ssu72-knockout DT40 cell lines. (A) Schematic representations of the chicken Ssu72 genomic fragment, knockout constructs, and configuration of the targeted alleles. Exons are shown as black boxes (E1–5), and the location of the 5′ probe used for Southern blotting is shown as a grey box. The double-headed arrows above the genes indicate length (in nucleotides). The XbaI and EcoRI restriction sites are indicated by vertical lines labeled X and R, respectively. (B) Southern blot analysis of wild-type (WT), heterozygous mutant (B15), homozygous mutant (P1, P2, P3), and unanticipated rearranged mutant (P10) clones. Genomic DNA obtained from each clone was digested with Xba I and Eco RI, and then hybridized with the 5′ probe shown in panel A. (C) RT-PCR analysis of the wild-type and mutant clones using primer pairs specific for the indicated gene. (D) Immunoblotting analysis of DT40 P3 (−/−) whole-cell extracts treated with Dox for the indicated times, using the indicated antibodies. Western blotting of β-actin was used as to confirm equal protein loading.

    Article Snippet: DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Promega), and subjected to RT-PCR using SYBR Premix Ex Taq II (TaKaRa) on an Mx3000P Real-time PCR system.

    Techniques: Knock-Out, Construct, Southern Blot, Labeling, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Clone Assay, Western Blot