polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879  (Thermo Fisher)


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    Thermo Fisher polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879
    Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of <t>Wilms</t> <t>tumor</t> nuclear <t>protein</t> <t>1</t> (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.
    Polyclonal Rabbit Anti Wilms Tumor Nuclear Protein 1 Wt1 Antibody Pa5 16879, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879 - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Roles of Sialic Acid, AXL, and MER Receptor Tyrosine Kinases in Mumps Virus Infection of Mouse Sertoli and Leydig Cells"

    Article Title: Roles of Sialic Acid, AXL, and MER Receptor Tyrosine Kinases in Mumps Virus Infection of Mouse Sertoli and Leydig Cells

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.01292

    Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of Wilms tumor nuclear protein 1 (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.
    Figure Legend Snippet: Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of Wilms tumor nuclear protein 1 (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.

    Techniques Used: Binding Assay, Isolation, Immunofluorescence, Staining, Wilms Tumor Assay, Incubation, Quantitative RT-PCR, Western Blot

    wilms tumor 1 wt1 protein  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
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    Thermo Fisher wilms tumor 1 wt1 protein
    Wilms Tumor 1 Wt1 Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wilms tumor 1 wt1 protein/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wilms tumor 1 wt1 protein - by Bioz Stars, 2023-02
    86/100 stars

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    Thermo Fisher polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879
    Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of <t>Wilms</t> <t>tumor</t> nuclear <t>protein</t> <t>1</t> (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.
    Polyclonal Rabbit Anti Wilms Tumor Nuclear Protein 1 Wt1 Antibody Pa5 16879, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti wilms tumor nuclear protein 1 wt1 antibody pa5 16879 - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher wilms tumor 1 wt1 protein
    Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of <t>Wilms</t> <t>tumor</t> nuclear <t>protein</t> <t>1</t> (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.
    Wilms Tumor 1 Wt1 Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wilms tumor 1 wt1 protein/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wilms tumor 1 wt1 protein - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of Wilms tumor nuclear protein 1 (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.

    Journal: Frontiers in Microbiology

    Article Title: Roles of Sialic Acid, AXL, and MER Receptor Tyrosine Kinases in Mumps Virus Infection of Mouse Sertoli and Leydig Cells

    doi: 10.3389/fmicb.2020.01292

    Figure Lengend Snippet: Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of Wilms tumor nuclear protein 1 (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.

    Article Snippet: Monoclonal rat anti-MER (14-5751-82) antibody and Polyclonal rabbit anti-Wilms tumor nuclear protein 1 (WT1) antibody (PA5-16879) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Binding Assay, Isolation, Immunofluorescence, Staining, Wilms Tumor Assay, Incubation, Quantitative RT-PCR, Western Blot