wild type f nucleatum atcc 23726  (ATCC)


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    Name:
    Fusobacterium nucleatum subsp nucleatum VPI 4351 1210
    Description:

    Catalog Number:
    23726
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    Structured Review

    ATCC wild type f nucleatum atcc 23726
    The Fap2 adhesin is involved in placental colonization. Wild-type F. <t>nucleatum</t> <t>ATCC</t> 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    https://www.bioz.com/result/wild type f nucleatum atcc 23726/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wild type f nucleatum atcc 23726 - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth"

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    Journal: Infection and Immunity

    doi: 10.1128/IAI.02838-14

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial
    Figure Legend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Techniques Used: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated
    Figure Legend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Techniques Used: Incubation

    Related Articles

    Mutagenesis:

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis
    Article Snippet: .. For F. nucleatum ATCC 23726 and its mutant derivatives, a portion of the Fusobacterium -specific fomA gene was amplified with Fn-F (forward) 5′-AGTTGCTCCAGCTTGGAGACCAAAT-3′ and Fn-R (reverse) 5′-AAGTTTACTTTTGTTAAAGTTTGTAATCTTCC-3′ primers. ..

    Amplification:

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis
    Article Snippet: .. For F. nucleatum ATCC 23726 and its mutant derivatives, a portion of the Fusobacterium -specific fomA gene was amplified with Fn-F (forward) 5′-AGTTGCTCCAGCTTGGAGACCAAAT-3′ and Fn-R (reverse) 5′-AAGTTTACTTTTGTTAAAGTTTGTAATCTTCC-3′ primers. ..

    other:

    Article Title: Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis
    Article Snippet: CFS from a type strain of F. nucleatum (ATCC 23726) also supported the growth of D. oralis , indicating its physiological dependence is not restricted to the strain it coenriched with, although we have not extended that analysis to additional strains or species.

    Binding Assay:

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis
    Article Snippet: .. Autoaggregation of bacteria and coaggregation of F. nucleatum ATCC 23726 with P. gingivalis displays a strain-specific profile Qualitative and quantitative autoaggregation and coaggregation assays revealed a strain-dependent binding profile of F. nucleatum ATCC 23726 with the seven different strains of P. gingivalis tested in this study. .. Robust interactions were observed with P. gingivalis strains 4612 (62%±8% coaggregation), T22 (54%±8% coaggregation) binding and ATCC 33277 (65%±17% coaggregation; ).

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    ATCC wild type f nucleatum atcc 23726
    F. nucleatum <t>ATCC</t> 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.
    Wild Type F Nucleatum Atcc 23726, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type f nucleatum atcc 23726/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    wild type f nucleatum atcc 23726 - by Bioz Stars, 2020-09
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    F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: F. nucleatum ATCC 23726 accelerates tumor growth and progression of lung metastasis. a Experimental scheme: AT3 breast cancer cell line was injected to the mammary fat pad of 6–7-week-old C57BL/6 mice. When tumor size reached 500 mm 3 , PBS vehicle (V), 5 × 10 7 F. nucleatum ATCC 23726 only (726), 5 × 10 7 F. nucleatum ATCC 23726 with metronidazole (726+MTZ.), metronidazole only (MTZ.), or 5 × 10 7 Fap2-deficient F. nucleatum K50 were injected into the tail vein. On days 2–3 post inoculation, mice were injected twice a day with metronidazole (M) or with PBS vehicle (V). On days 4–7 post inoculation, mice were injected once a day with metronidazole or with PBS. Eight days after inoculation, mice were sacrificed, and tumor and lungs harvested. Breast cancer tissues were weighed, and lung metastases were counted. b Tumor weights normalized as the percentage compared with the average tumor weight in the PBS group (vehicle control) in each experiment (set as 100% tumor weight). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 3.48 × 10 −5 , ** p = 0.002. Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 21, MTZ. n = 17, K50 n = 7, 726 n = 19, 726+MTZ. n = 12. c Representative tumors post-harvest. d Representative image of lung metastases. Scale bar, 500 µm in the large image, 250 µm in the indicated inset. e Lung metastasis rank [number of metastases per lung area (pixel 2 )] calculated as percentage compared with the average metastasis rank in the vehicle control group of each experiment (set as 100% metastasis rank). Each symbol represents one mouse. Presented results are of three independent experiments. **** p = 4.08 × 10 −5 , * p = 0.0177, n.s.: non-significant, Holm’s correction, one-tailed Mann–Whitney. Vehicle n = 20, MTZ. n = 17, K50 n = 7, 726 n = 18, 726+met n = 12. f Images taken by fluorescence binocular of lung metastases in four mice implanted with GFP-expressing AT3 cells as described above. Two tumor-bearing mice were inoculated with F. nucleatum and two were treated by PBS vehicle. Scale bar, 1000 µm. Source data are provided as a Source data file.

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Mouse Assay, One-tailed Test, MANN-WHITNEY, Fluorescence, Expressing

    AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Mouse Assay, Flow Cytometry, Two Tailed Test, MANN-WHITNEY, Expressing, Infection, Zymography, Multiple Displacement Amplification, Cell Culture

    F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: F. nucleatum colonizes mammary cancer in a Fap2-mediated mechanism. a Orthotropic 4T1 BALB/c mouse mammary cancer model. When reaching 500 mm 3 size, 5 × 10 7 F. nucleatum ATCC 23726 were IV injected. Twenty-four hours post inoculation, breast cancer and normal tissue from an adjacent mammary were harvested. b Representative images and sum of fluorescence intensity of binding of FITC-labeled PNA (green) to tumor and normal tissue stained with Hoechst dye (blue). Each paired symbols represents one mouse ( n = 7), ** p = 0.0078, one-tailed, Wilcoxon matched-paired signed rank test. c Relative F. nucleatum gDNA abundance (2 −ΔCt ) in breast tissue from tumor-free mice (No tumor, n = 3), and in tumor and matching normal tissue of 4T1 tumor-bearing mice ( n = 5) all inoculated with F. nucleatum ATCC 23726. * p = 0.0358 Holm-corrected one-tailed Mann–Whitney test. * p = 0.0313, one-tailed, Wilcoxon matched-paired signed rank test (for paired normal adjacent-tumor samples). d Orthotropic AT3 C57BL/6 mouse mammary cancer model. When reaching 500 mm 3 , mice were inoculated with 5 × 10 7 F. nucleatum ATCC 23726 (726; blue), 5 × 10 7 Fap2 mutant K50 (MUT K50; red) or with 5 × 10 7 P. gingivalis (Pg; black). Twenty-four hours later, normal mammary and breast cancer tissue were harvested from each mice and bacteria quantified. e Bacterial abundance (CFU/g tissue), and relative bacterial gDNA abundance (2 −ΔCt ) in tumor and normal tissue of AT3 tumor-bearing mice inoculated with P. gingivalis ( Pg , n = 9), K50 ( n = 12), or F. nucleatum ATCC 23726 (726, n = 12). Each symbol represents one mouse. * p = 0.0156 one-tailed, Wilcoxon matched-paired signed rank test. *** p = 0.0006, ** p = 0.001598. Holm-corrected one-tailed Mann–Whitney test (left panel). **** p

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Injection, Fluorescence, Binding Assay, Labeling, Staining, One-tailed Test, Mouse Assay, MANN-WHITNEY, Mutagenesis

    Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: Attachment of F. nucleatum ATCC 23726 to breast cancer is Fap2-mediated and Gal-GalNAc dependent. a Representative images (left panels, scale bar 20 µm) and quantitative analysis (Fn/mm 2 ) (right panel) of binding of Cy3-labeled (white) WT F. nucleatum ATCC 23726 (726) and of its Cy5-labeled (red) Fap2-inactivated isogenic mutant K50, to Hoechst-stained (blue) human breast cancer TMAs (HBre-Duc060CS-01 and BR1006). Results in the right panel are classified by tissue type (normal n = 35, benign n = 7, malignant n = 64) and bacterial strain (K50, 726). Each core is measured twice, once for each of the strains in the appropriate tissue type (as demonstrated in the pictures). When an individual contributed a single core to the experiment, the two observations are shown as circles (●); when she contributed two cores, these were to the normal type and the malignant type, and the four observations are depicted as plus signs (+). The six classes were compared against each other in pairs: the exact two-tailed Wilcoxon test was used for pairs that were from the same core, the exact two-tailed Mann–Whitney test for independent samples. Where the same hypothesis was tested on more than one pair, and the individual pairs were mutually independent, the corresponding p -values were combined by Fisher’s method. Wherever the pairs were not independent, the multiple comparisons were adjusted for using the Holm modification of the Bonferroni correction. **** p

    Article Snippet: Supporting the predicted Fap2-mediated recognition of Gal-GalNAc, wild-type F. nucleatum ATCC 23726 showed significantly higher attachment than the K50 and D22 mutants (Fig. ).

    Techniques: Binding Assay, Labeling, Mutagenesis, Staining, Two Tailed Test, MANN-WHITNEY, Modification

    The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Journal: Infection and Immunity

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    doi: 10.1128/IAI.02838-14

    Figure Lengend Snippet: The Fap2 adhesin is involved in placental colonization. Wild-type F. nucleatum ATCC 23726 or the hemagglutination-deficient mutant K50 was injected into the tail veins of pregnant mice. After 24 h, the placentas were harvested and homogenized, and bacterial

    Article Snippet: Wild-type F. nucleatum ATCC 23726, the three hemagglutination-deficient mutants, and a randomly selected control mutant, J78, were stained with carboxyfluorescein succinimidyl ester (CFSE) and incubated with HEK 293T cells.

    Techniques: Mutagenesis, Injection, Mouse Assay

    F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Journal: Infection and Immunity

    Article Title: Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

    doi: 10.1128/IAI.02838-14

    Figure Lengend Snippet: F. nucleatum ATCC 23726 hemagglutination is inhibited by d -galactose but not by l -arginine. (A) Bacteria incubated with 2% sheep red blood cells (RBC) in the absence and presence of 6 mM d -galactose (Gal) or 50 mM l -arginine (Arg). (B) Erythrocytes incubated

    Article Snippet: Wild-type F. nucleatum ATCC 23726, the three hemagglutination-deficient mutants, and a randomly selected control mutant, J78, were stained with carboxyfluorescein succinimidyl ester (CFSE) and incubated with HEK 293T cells.

    Techniques: Incubation

    Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Journal: International Journal of Oral Science

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    doi: 10.1038/ijos.2016.27

    Figure Lengend Snippet: Quantitative coaggregation assay between Fusobacterium nucleatum ATCC 23726 and mutant derivatives in outer membrane proteins with Porphyromonas gingivalis strains. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared with coaggregation reaction of the wild type with the respective P. gingivalis partner strains set as 100%. Data represent the means and standard deviation of at least three independent experiments. * P

    Article Snippet: Our results for integration of P. gingivalis 4612 and T22 into fusobacterial biofilms formed by F. nucleatum ATCC 23726 wild-type and its mutant derivative lacking Fap2, RadD or both as well as FN1893 confirmed the importance of Fap2 and RadD for attachment of 4612 to ATCC 23726 ( ).

    Techniques: Mutagenesis, Standard Deviation

    Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Journal: International Journal of Oral Science

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    doi: 10.1038/ijos.2016.27

    Figure Lengend Snippet: Porphyromonas gingivalis integration in dual-species biofilms with Fusobacterium nucleatum ATCC 23726 ΔFap2, ΔRadD and ΔFap2/ΔRadD outer membrane proteins mutant derivatives. Biofilm integration is given as a percentage relative to biofilm integration measured with WT F. nucleatum ATCC 23726. At least three independent experiments were performed per strain combination. Data represent the means and standard deviation of at least three independent experiments. * P

    Article Snippet: Our results for integration of P. gingivalis 4612 and T22 into fusobacterial biofilms formed by F. nucleatum ATCC 23726 wild-type and its mutant derivative lacking Fap2, RadD or both as well as FN1893 confirmed the importance of Fap2 and RadD for attachment of 4612 to ATCC 23726 ( ).

    Techniques: Mutagenesis, Standard Deviation

    Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Journal: International Journal of Oral Science

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    doi: 10.1038/ijos.2016.27

    Figure Lengend Snippet: Quantitative autoaggregation levels of bacterial strains and coaggregation levels between Fusobacterium nucleatum ATCC 23726 and seven different Porphyromonas gingivalis strains. Data are expressed as percentage aggregation and represent the means and standard deviation of at least three independent experiments.

    Article Snippet: Our results for integration of P. gingivalis 4612 and T22 into fusobacterial biofilms formed by F. nucleatum ATCC 23726 wild-type and its mutant derivative lacking Fap2, RadD or both as well as FN1893 confirmed the importance of Fap2 and RadD for attachment of 4612 to ATCC 23726 ( ).

    Techniques: Standard Deviation

    Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Journal: International Journal of Oral Science

    Article Title: Characterization of Fusobacterium nucleatum ATCC 23726 adhesins involved in strain-specific attachment to Porphyromonas gingivalis

    doi: 10.1038/ijos.2016.27

    Figure Lengend Snippet: Quantitative inhibition of coaggregation assay between Fusobacterium nucleatum ATCC 23726 and Porphyromonas gingivalis strains in the presence of inhibitors. ( a ) P. gingivalis 4612 with F. nucleatum ATCC 23726; ( b ) P. gingivalis T22 with F. nucleatum ATCC 23726; ( c ) P. gingivalis ATCC 33277 with F. nucleatum ATCC 23726. Data are expressed as relative percentage coaggregation compared to coaggregation reaction of the partner strains in buffer set as 100% and represent the means and standard deviation of at least three independent experiments. * P

    Article Snippet: Our results for integration of P. gingivalis 4612 and T22 into fusobacterial biofilms formed by F. nucleatum ATCC 23726 wild-type and its mutant derivative lacking Fap2, RadD or both as well as FN1893 confirmed the importance of Fap2 and RadD for attachment of 4612 to ATCC 23726 ( ).

    Techniques: Inhibition, Standard Deviation