england biolabs low  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs england biolabs low
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    England Biolabs Low, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/england biolabs low/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    england biolabs low - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase–Cas1 fusion protein"

    Article Title: Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase–Cas1 fusion protein

    Journal: Science Advances

    doi: 10.1126/sciadv.adk8791

    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Figure Legend Snippet: ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).

    Techniques Used: DNA Synthesis, Sequencing, Blocking Assay, Labeling, Extraction, Incubation, Synthesized

    low molecular weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    New England Biolabs low molecular weight dna ladder
    Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight dna ladder/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight dna ladder - by Bioz Stars, 2024-06
    86/100 stars

    Images

    england biolabs low  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs england biolabs low
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    England Biolabs Low, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/england biolabs low/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    england biolabs low - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase–Cas1 fusion protein"

    Article Title: Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase–Cas1 fusion protein

    Journal: Science Advances

    doi: 10.1126/sciadv.adk8791

    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Figure Legend Snippet: ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).

    Techniques Used: DNA Synthesis, Sequencing, Blocking Assay, Labeling, Extraction, Incubation, Synthesized

    low molecular weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs low molecular weight dna ladder
    Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight dna ladder - by Bioz Stars, 2024-06
    96/100 stars

    Images

    weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs weight dna ladder
    Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/weight dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    weight dna ladder - by Bioz Stars, 2024-06
    96/100 stars

    Images

    quick-load purple low molecular weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs quick-load purple low molecular weight dna ladder
    Quick Load Purple Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick-load purple low molecular weight dna ladder/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick-load purple low molecular weight dna ladder - by Bioz Stars, 2024-06
    93/100 stars

    Images

    low molecular weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs low molecular weight dna ladder
    Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight dna ladder - by Bioz Stars, 2024-06
    96/100 stars

    Images

    low molecular weight dna ladder  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    New England Biolabs low molecular weight dna ladder
    Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight dna ladder - by Bioz Stars, 2024-06
    96/100 stars

    Images

    molecular weight marker  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs molecular weight marker
    Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight marker/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molecular weight marker - by Bioz Stars, 2024-06
    95/100 stars

    Images

    molecular weight marker  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs molecular weight marker
    Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight marker/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molecular weight marker - by Bioz Stars, 2024-06
    95/100 stars

    Images

    molecular weight marker  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs molecular weight marker
    Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight marker/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molecular weight marker - by Bioz Stars, 2024-06
    95/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    New England Biolabs england biolabs low
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    England Biolabs Low, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/england biolabs low/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    england biolabs low - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    86
    New England Biolabs low molecular weight dna ladder
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low molecular weight dna ladder/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight dna ladder - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    96
    New England Biolabs weight dna ladder
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/weight dna ladder/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    weight dna ladder - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    93
    New England Biolabs quick-load purple low molecular weight dna ladder
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Quick Load Purple Low Molecular Weight Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick-load purple low molecular weight dna ladder/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick-load purple low molecular weight dna ladder - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    95
    New England Biolabs molecular weight marker
    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England <t>Biolabs).</t> ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).
    Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight marker/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molecular weight marker - by Bioz Stars, 2024-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).

    Journal: Science Advances

    Article Title: Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase–Cas1 fusion protein

    doi: 10.1126/sciadv.adk8791

    Figure Lengend Snippet: ( A ) DNA synthesis reactions were done by incubating WT RT-Cas1/Cas2 (left) or RT∆-Cas1/Cas2 (right) with 50-nt RNA or DNA oligonucleotide templates (250 nM) containing a 3′-proximal CCC sequence (R50CCC and D50CCC, respectively) without or with a 3′-ddC blocking group and 32 P-labeled dNTPs (20 μM [α- 32 P]-dCTP + 500 μM dATP, dGTP, and dTTP) in reaction medium containing 10 mM MgCl 2 ± 1 mM MnCl 2 for 1 hour at 25°C. After phenol extraction, the samples were analyzed in a denaturing 20% polyacrylamide gel, which was dried and scanned with a phosphorimager. Lane M, 5′-labeled 10-nt ssDNA ladder size markers in a parallel lane, with a darker exposure of that lane appended on the left. ( B ) RT-Cas1/Cas2 cDNA synthesis reactions done as in (A) with 3′-blocked 50-nt DNA and RNA templates containing a 3′ proximal CCC sequence. After cleanup with a Zymo Oligo Clean and Concentrator kit, the products were incubated for 20 min at 37°C in the absence or presence of RNase H or RNase A, followed by protease K digestion and analysis in a nondenaturing 15% polyacrylamide gel. Lane M, 5′-labeled low molecular DNA ladder size markers (New England Biolabs). ( C ) RT-Cas1/Cas2 DNA synthesis reactions with 3′-blocked DNA and RNA templates containing a 3′ proximal CCC or CGC sequence. Reactions were done and analyzed in a denaturing 20% polyacrylamide gel as in (A). The schematics to the right of the gels in (A) to (C) depict the labeled cDNAs (blue line with stars) synthesized from RNA or DNA templates (red). All experiments shown in the figure were repeated with similar results (fig. S11).

    Article Snippet: Samples were mixed at a 2:1 ratio with loading dye and analyzed on an 8% polyacrylamide/8 M urea gel against a 5′-labeled New England Biolabs low–molecular weight DNA ladder run in a parallel lane.

    Techniques: DNA Synthesis, Sequencing, Blocking Assay, Labeling, Extraction, Incubation, Synthesized