human ipsc lines fx11 7  (WiCell Research Institute Inc)

Journal: Molecular Psychiatry

Article Title: Autophagy controls the hippocampal postsynaptic organization and affects cognition in a mouse model of Fragile X syndrome

doi: 10.1038/s41380-025-03207-6

Figure Lengend Snippet: A Information of human iPSCs. B Average percentage of methylation on CpGs in the indicated promotor region of Fmr1 gene. n = 3 samples in each group. C Neurons were differentiated from unaffected (control) and FXS iPSCs. FMRP expression was examined by immunostaining of FMRP and neuronal marker Tuj-1. Scale bar, 15 µm. D iPSCs-derived neurons were treated with DMSO as vehicle or Rilmenidine (Ril) (10 µM for 6 hr), and immunostained with p62 together with Tuj-1. Scale bar, 15 µm. E Summary bar graph shows fluorescent intensity of p62 puncta in D (normalized to the value of control). F, H iPSCs-derived neurons were treated with DMSO as vehicle or Rilmenidine (Ril) (10 µM for 6 hr), and immunostained with eIF4G1/PSD-95 together with Tuj-1. Scale bar, 20 µm. G, I Summary bar graphs show fluorescent intensities of eIF4G1 and PSD-95 puncta. J F/G-actin ratio was assessed by Western blot with lysates from iPSCs derived neurons. K Bar graph shows summary ratio of F/G (normalized to the value of control, n = 4 experiments). L iPSCs derived neurons were treated with Veh or Rilmenidine (10 µM) for 6 h and imaged with F-actin. Scale bar, 3 µm. M Summary bar graph shows fluorescent intensity of F-actin. Significance was calculated by ANOVA followed by a Tukey’s test. * p < 0.05, ** p < 0.01. Values reflect mean ± s.e.m. Each circle in E, G, I, K and M represents data from an individual experiment.

Article Snippet: Human FXS iPSCs (WC005i-FX11-7) and (WC005i-FX08-23) and control iPSCs (WC008i-C603-4) were purchased from WiCell Research Institute (WI, USA) [ ].

Techniques: Methylation, Control, Expressing, Immunostaining, Marker, Derivative Assay, Western Blot