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Transgenomic wave dna fragment analysis system
<t>WAVE</t> analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following <t>DNA</t> templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.
Wave Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wave dna fragment analysis system/product/Transgenomic
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wave dna fragment analysis system - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "Detection of Plasmid-Mediated AmpC ?-Lactamase Genes in Clinical Isolates by Using Multiplex PCR"

Article Title: Detection of Plasmid-Mediated AmpC ?-Lactamase Genes in Clinical Isolates by Using Multiplex PCR

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.40.6.2153-2162.2002

WAVE analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following DNA templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.
Figure Legend Snippet: WAVE analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following DNA templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.

Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Amplification, Activated Clotting Time Assay, Marker, Agarose Gel Electrophoresis

2) Product Images from "Toll-Like Receptor 4 (TLR4) and Typhoid Fever in Vietnam"

Article Title: Toll-Like Receptor 4 (TLR4) and Typhoid Fever in Vietnam

Journal: PLoS ONE

doi: 10.1371/journal.pone.0004800

Mutation detection in the T4_promoter fragment. Three polymorphisms were detected by dHPLC in the T4_promoter fragment and were verified by DNA sequencing. The four dHPLC graphs correspond to the wild-type sequence (A), SNPs T-441C (B), SNP A-271G (C) and SNP G-259C (D). The wild-type pattern is visible in all dHPLC graphs as a 1 peak trace. SNP T-441C is identified by an unequal height 2 peak trace (B), SNP A-271G is identified by an equal height 2 peak trace (C) and SNP G-259C is identified by a 3 peak trace (D). The positions of the sequence variants in the T4_promoter fragment identified by dHPLC were determined by DNA sequencing. All SNPs were present in the heterozygous state.
Figure Legend Snippet: Mutation detection in the T4_promoter fragment. Three polymorphisms were detected by dHPLC in the T4_promoter fragment and were verified by DNA sequencing. The four dHPLC graphs correspond to the wild-type sequence (A), SNPs T-441C (B), SNP A-271G (C) and SNP G-259C (D). The wild-type pattern is visible in all dHPLC graphs as a 1 peak trace. SNP T-441C is identified by an unequal height 2 peak trace (B), SNP A-271G is identified by an equal height 2 peak trace (C) and SNP G-259C is identified by a 3 peak trace (D). The positions of the sequence variants in the T4_promoter fragment identified by dHPLC were determined by DNA sequencing. All SNPs were present in the heterozygous state.

Techniques Used: Mutagenesis, DNA Sequencing, Sequencing

3) Product Images from "Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM"

Article Title: Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

Journal: BMC Medical Genetics

doi: 10.1186/1471-2350-9-53

Result of DHPLC . DHPLC results of two families were illustrated. a) Family 85. b) Family 139. The wild type DNA showed homoduplex. The carrier DNA would show heteroduplex peak just as affected male after mixing with normal DNA.
Figure Legend Snippet: Result of DHPLC . DHPLC results of two families were illustrated. a) Family 85. b) Family 139. The wild type DNA showed homoduplex. The carrier DNA would show heteroduplex peak just as affected male after mixing with normal DNA.

Techniques Used:

Related Articles

High Performance Liquid Chromatography:

Article Title: Novel Mutations in MLH1 and MSH2 Genes in Mexican Patients with Lynch Syndrome
Article Snippet: The fragments were then visualized by gel electrophoresis in a 2% agarose gel, stained with ethidium bromide. .. Denaturing High Performance Liquid Chromatography (dHPLC) was undertaken using the Hitachi WAVE® DNA fragment analysis system HSX-3500 (Transgenomic™ ). .. An aliquot (5 µ L) of the PCR product was directly injected into a DNA Sep column.

Polymerase Chain Reaction:

Article Title: Matrix Metalloproteinase-9 (MMP-9) polymorphisms in patients with cutaneous malignant melanoma
Article Snippet: In the analysis we determined: 1) the steric distortions and hydrogen bond losses caused by the polymorphisms; 2) the effect of the residue changes on the interactions with known ligands (metal cofactors, synthetic and natural inhibitors); and 3) the effect on conserved or specificity residues of four superfamilies of MMP-9 domains (MMP N-terminal domain, catalytic domain, fibronectin type II domain, hemopexin domain) by multiple sequence analysis [ ]. .. Genotyping All genotyping was done with PCR-based methods and included: melting temperature analysis [ ] coupled to the LightTyper instrument (Roche Applied Science, Indianapolis, USA), pyrosequencing [ ] with the PSQ™ 96 MA or PSQ™HS 96A instruments (Biotage AB, Uppsala, Sweden), fragment size analysis [ ] by an ABI PRISM® 3100 Genetic Analyzer (Applied Biosystems, Foster City, USA), and heteroduplex analysis [ , ] using the Wave DNA Fragment Analysis System (Transgenomic, Omaha, USA). .. The PCR primers and PCR conditions are listed in the Additional file .

Article Title: Resequencing of genes for transforming growth factor ?1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy
Article Snippet: As the TGFBR1 and TGFBR2 gene sequences cover approximately 45 kb and 84 kb respectively from start to stop codon, only the coding regions of these genes (including all exons, exon-intron boundaries and untranslated regions) were screened to prioritise the identification of potentially functional gene variants. .. Each PCR product was then evaluated using WaveMaker v3.4 software (Transgenomic Ltd, Crewe, UK) and analysed on the WAVE™ (dHPLC) DNA Fragment Analysis System (Transgenomic Ltd) following the manufacturer's recommendations. .. Differentially separating fragments (representing DNA variants) were bidirectionally sequenced to identify variants using an ABI PRISM® 3100 Genetic Analyser (Applied Biosystems, Warrington, UK).

Software:

Article Title: Resequencing of genes for transforming growth factor ?1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy
Article Snippet: As the TGFBR1 and TGFBR2 gene sequences cover approximately 45 kb and 84 kb respectively from start to stop codon, only the coding regions of these genes (including all exons, exon-intron boundaries and untranslated regions) were screened to prioritise the identification of potentially functional gene variants. .. Each PCR product was then evaluated using WaveMaker v3.4 software (Transgenomic Ltd, Crewe, UK) and analysed on the WAVE™ (dHPLC) DNA Fragment Analysis System (Transgenomic Ltd) following the manufacturer's recommendations. .. Differentially separating fragments (representing DNA variants) were bidirectionally sequenced to identify variants using an ABI PRISM® 3100 Genetic Analyser (Applied Biosystems, Warrington, UK).

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    Transgenomic wave dna fragment analysis system
    <t>WAVE</t> analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following <t>DNA</t> templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.
    Wave Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave dna fragment analysis system/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wave dna fragment analysis system - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Transgenomic ip hplc system
    Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and <t>SNuPE/HPLC</t> in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic <t>DNA</t> (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.
    Ip Hplc System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ip hplc system/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ip hplc system - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Transgenomic wave 3500 dna fragment analysis system
    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the <t>WAVE</t> 3500 <t>DNA</t> Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
    Wave 3500 Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave 3500 dna fragment analysis system/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wave 3500 dna fragment analysis system - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    WAVE analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following DNA templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection of Plasmid-Mediated AmpC ?-Lactamase Genes in Clinical Isolates by Using Multiplex PCR

    doi: 10.1128/JCM.40.6.2153-2162.2002

    Figure Lengend Snippet: WAVE analysis. (A) Chromatogram obtained by using multiplex PCR products amplified from the following DNA templates (bottom to top): FOX-1, ACT-1, ACC, DHA-1, LAT-1, MOX-1, combination of the six DNA templates listed above, and DNA marker pUC18. (B) Agarose gel electrophoresis of multiplex PCR products obtained by using three different combinations of DNA templates: 2 Templates, FOX-1 and ACT-1; 4 Templates, MOX-1, LAT-1, DHA-1, and ACC; and 6 Templates, combination of the six templates listed above. M, 100-bp ladder.The amplified product from each PCR is indicated on the left, and the size of the marker in base pairs is shown on the right.

    Article Snippet: Following PCR amplification, the products were analyzed by using the WAVE DNA fragment analysis system with Wavemaker Software (Transgenomic, Inc., Omaha, Nebr.).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Amplification, Activated Clotting Time Assay, Marker, Agarose Gel Electrophoresis

    Result of DHPLC . DHPLC results of two families were illustrated. a) Family 85. b) Family 139. The wild type DNA showed homoduplex. The carrier DNA would show heteroduplex peak just as affected male after mixing with normal DNA.

    Journal: BMC Medical Genetics

    Article Title: Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

    doi: 10.1186/1471-2350-9-53

    Figure Lengend Snippet: Result of DHPLC . DHPLC results of two families were illustrated. a) Family 85. b) Family 139. The wild type DNA showed homoduplex. The carrier DNA would show heteroduplex peak just as affected male after mixing with normal DNA.

    Article Snippet: Mutational screening, performed on all amplified fragments from each patient, was carried out via denaturing high-performance liquid chromatography (DHPLC) on a Wave® DNA Fragment Analysis System (Transgenomic Inc., San Jose, CA) with a DNASep column (Transgenomic).

    Techniques:

    Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and SNuPE/HPLC in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic DNA (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.

    Journal: The Journal of Experimental Medicine

    Article Title: Promiscuous gene expression in thymic epithelial cells is regulated at multiple levels

    doi: 10.1084/jem.20050471

    Figure Lengend Snippet: Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and SNuPE/HPLC in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic DNA (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.

    Article Snippet: Extension products were separated on an IP-HPLC system (WAVE DNA Fragment Analysis System; Transgenomics).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, High Performance Liquid Chromatography, Polymerase Chain Reaction

    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.

    Journal: Neural Regeneration Research

    Article Title: The human δ2 glutamate receptor gene is not mutated in patients with spinocerebellar ataxia

    doi: 10.4103/1673-5374.133173

    Figure Lengend Snippet: Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.

    Article Snippet: DHPLC analysis for GRID2 was performed using the WAVE 3500 DNA Fragment Analysis System (Transgenomic Inc., Omaha, Nebraska, USA) with a DNASep cartridge.

    Techniques: Sequencing, High Performance Liquid Chromatography, Polymerase Chain Reaction, Mutagenesis