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Transgenomic wave 3500 dna fragment analysis system
Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the <t>WAVE</t> 3500 <t>DNA</t> Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
Wave 3500 Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wave 3500 dna fragment analysis system/product/Transgenomic
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wave 3500 dna fragment analysis system - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "The human δ2 glutamate receptor gene is not mutated in patients with spinocerebellar ataxia"

Article Title: The human δ2 glutamate receptor gene is not mutated in patients with spinocerebellar ataxia

Journal: Neural Regeneration Research

doi: 10.4103/1673-5374.133173

Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
Figure Legend Snippet: Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.

Techniques Used: Sequencing, High Performance Liquid Chromatography, Polymerase Chain Reaction, Mutagenesis

Related Articles

High Performance Liquid Chromatography:

Article Title: Novel Mutations in MLH1 and MSH2 Genes in Mexican Patients with Lynch Syndrome
Article Snippet: The fragments were then visualized by gel electrophoresis in a 2% agarose gel, stained with ethidium bromide. .. Denaturing High Performance Liquid Chromatography (dHPLC) was undertaken using the Hitachi WAVE® DNA fragment analysis system HSX-3500 (Transgenomic™ ). .. An aliquot (5 µ L) of the PCR product was directly injected into a DNA Sep column.

Methylation:

Article Title: Characterization of human gastric carcinoma-related methylation of 9 miR CpG islands and repression of their expressions in vitro and in vivo
Article Snippet: The PCR products were then analyzed quantitatively by DHPLC on the WAVE® DNA Fragment Analysis System [ ]. .. Elution profiles of miR-9-3 , miR-200b , and miR-203 methylation was analyzed with an ultraviolet detector; other miR gene methylation was detected with the post column HSX-3500 Accessory (Transgenomic, Inc., Omaha, USA) and a high–sensitivity fluorescence (FL) detector (excitation at 450 nm, emission at 520 nm) [ ]. .. Methylated and unmethylated miR gene PCR products were separated by a DNASep® analytical column (Transgenomic) at the corresponding partial denaturing temperature (Additional file : Table S3 and Figure S2-10).

Fluorescence:

Article Title: Characterization of human gastric carcinoma-related methylation of 9 miR CpG islands and repression of their expressions in vitro and in vivo
Article Snippet: The PCR products were then analyzed quantitatively by DHPLC on the WAVE® DNA Fragment Analysis System [ ]. .. Elution profiles of miR-9-3 , miR-200b , and miR-203 methylation was analyzed with an ultraviolet detector; other miR gene methylation was detected with the post column HSX-3500 Accessory (Transgenomic, Inc., Omaha, USA) and a high–sensitivity fluorescence (FL) detector (excitation at 450 nm, emission at 520 nm) [ ]. .. Methylated and unmethylated miR gene PCR products were separated by a DNASep® analytical column (Transgenomic) at the corresponding partial denaturing temperature (Additional file : Table S3 and Figure S2-10).

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    Transgenomic wave 3500 dna fragment analysis system
    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the <t>WAVE</t> 3500 <t>DNA</t> Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
    Wave 3500 Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave 3500 dna fragment analysis system/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wave 3500 dna fragment analysis system - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Transgenomic wave dna fragment analysis system
    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the <t>WAVE</t> 3500 <t>DNA</t> Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
    Wave Dna Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave dna fragment analysis system/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wave dna fragment analysis system - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Transgenomic wave dna fragment analysis system hsx 3500
    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the <t>WAVE</t> 3500 <t>DNA</t> Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.
    Wave Dna Fragment Analysis System Hsx 3500, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave dna fragment analysis system hsx 3500/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wave dna fragment analysis system hsx 3500 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.

    Journal: Neural Regeneration Research

    Article Title: The human δ2 glutamate receptor gene is not mutated in patients with spinocerebellar ataxia

    doi: 10.4103/1673-5374.133173

    Figure Lengend Snippet: Sequence alterations detected by denaturing high-performance liquid chromatography analysis. Analysis of GRID2 PCR products for exon 5 (A) or exon 9 (B, C) (including flanking sequences) was performed using the WAVE 3500 DNA Fragment Analysis System. (A) Double peaks at the non-denaturing temperature (50°C) (upper) and multiple peaks at partially denaturing temperature (54.9°C) (lower) were detected in exon 5, indicating the presence of a mixture of two fragments with different sizes. (B) Exon 9: Homogeneous peak at the non-denaturing temperature (50°C). (C) Exon 9: Heterozygous duplexes at partially denaturing temperature (58.5°C), indicating the presence of a point mutation within the exon.

    Article Snippet: DHPLC analysis for GRID2 was performed using the WAVE 3500 DNA Fragment Analysis System (Transgenomic Inc., Omaha, Nebraska, USA) with a DNASep cartridge.

    Techniques: Sequencing, High Performance Liquid Chromatography, Polymerase Chain Reaction, Mutagenesis