vybrant cfda se cell tracer  (Thermo Fisher)


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    Name:
    Vybrant CFDA SE Cell Tracer Kit
    Description:
    Vybrant CFDA SE Cell Tracer Kit is used in fluorescent analyses as a long term tracer of cells This method is commonly used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating • Superior performance allows for bright uniform staining of cells• Long term retention dye is well retained in cells for several days post stain• Non toxic minimal cytotoxicity for in vivo and in vitro assays• Simple robust staining protocolSuperior Fluorescent StainingSuccessful tracing of cells requires an extremely bright dye to distinguish fluorescently labeled cells from auto fluorescence The intense fluorescent staining provided by Vybrant CFDA SE Cell Tracer Kit enables the visualization of labeled cells for several days post stain making it an ideal stain for cell tracing studies based on dye dilution Long Term Signal RetentionVybrant CFDA SE dye easily crosses the plasma membrane and covalently binds inside cells where the stable well retained fluorescent dye is designed to provide a consistent signal even after several days in a cell culture environment Non ToxicVybrant CFDA SE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity with minimal observed effect on the proliferative ability or biology of cells Researchers have used CFDA SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail Simple Robust Staining ProtocolThe Vybrant CFDA SE Cell Tracer Kit contains convenient single use vials of dry dye to permit small scale experiments without preparing excess quantities of dye thereby extending the shelf life of the dye not dissolved in solution A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use To stain 1 mL of cells in protein free medium 1 µl of this stock solution is typically used Cells should be stained for 20 minutes at room temperature with gentle agitation A brief wash with complete medium will then quench any dye remaining in solution
    Catalog Number:
    V12883
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Adhesion|Cell Analysis|Cell Proliferation|Cellular Imaging|Cellular Toxicology Assays|Chemotaxis & Cell Migration|Chemotaxis & Cell Migration|General Cell Tracing|High-Content Screening (HCS)|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Industrial & Applied Science|Microbial Tracking|Pharma & Biopharma|Target-Based ADME⁄Tox Assays|Cell Tracing & Tracking|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher vybrant cfda se cell tracer
    Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of <t>Vybrant</t> <t>CFDA</t> SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P
    Vybrant CFDA SE Cell Tracer Kit is used in fluorescent analyses as a long term tracer of cells This method is commonly used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating • Superior performance allows for bright uniform staining of cells• Long term retention dye is well retained in cells for several days post stain• Non toxic minimal cytotoxicity for in vivo and in vitro assays• Simple robust staining protocolSuperior Fluorescent StainingSuccessful tracing of cells requires an extremely bright dye to distinguish fluorescently labeled cells from auto fluorescence The intense fluorescent staining provided by Vybrant CFDA SE Cell Tracer Kit enables the visualization of labeled cells for several days post stain making it an ideal stain for cell tracing studies based on dye dilution Long Term Signal RetentionVybrant CFDA SE dye easily crosses the plasma membrane and covalently binds inside cells where the stable well retained fluorescent dye is designed to provide a consistent signal even after several days in a cell culture environment Non ToxicVybrant CFDA SE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity with minimal observed effect on the proliferative ability or biology of cells Researchers have used CFDA SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail Simple Robust Staining ProtocolThe Vybrant CFDA SE Cell Tracer Kit contains convenient single use vials of dry dye to permit small scale experiments without preparing excess quantities of dye thereby extending the shelf life of the dye not dissolved in solution A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use To stain 1 mL of cells in protein free medium 1 µl of this stock solution is typically used Cells should be stained for 20 minutes at room temperature with gentle agitation A brief wash with complete medium will then quench any dye remaining in solution
    https://www.bioz.com/result/vybrant cfda se cell tracer/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vybrant cfda se cell tracer - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Increased IGF-IEc expression and mechano-growth factor production in intestinal muscle of fibrostenotic Crohn's disease and smooth muscle hypertrophy"

    Article Title: Increased IGF-IEc expression and mechano-growth factor production in intestinal muscle of fibrostenotic Crohn's disease and smooth muscle hypertrophy

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00414.2014

    Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of Vybrant CFDA SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P
    Figure Legend Snippet: Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of Vybrant CFDA SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P

    Techniques Used: Imaging, Incubation

    Related Articles

    Purification:

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells
    Article Snippet: Where indicated, specific inhibitors of Arginase I ( N -Hydroxy-nor- l -arginine, nor-NOHA, 1 mM, Calbiochem), indoleamine 2,3-dioxygenase (1-Methyl- d -tryptophan, 1-MTD, 1 mM, Sigma-Aldrich) or nitric oxide synthases ( N -Monomethyl- l -arginene, Monoacetate Salt, l -NMMA, 1 mM, Calbiochem) were used. .. In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction. .. Labeled PBMC were then cultured with purified PMN-MDSC at 1:1 ratio and stimulated with Staphylococcus enterotoxin B (SEB, 200 ng/mL, Sigma-Aldrich).

    Labeling:

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells
    Article Snippet: Where indicated, specific inhibitors of Arginase I ( N -Hydroxy-nor- l -arginine, nor-NOHA, 1 mM, Calbiochem), indoleamine 2,3-dioxygenase (1-Methyl- d -tryptophan, 1-MTD, 1 mM, Sigma-Aldrich) or nitric oxide synthases ( N -Monomethyl- l -arginene, Monoacetate Salt, l -NMMA, 1 mM, Calbiochem) were used. .. In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction. .. Labeled PBMC were then cultured with purified PMN-MDSC at 1:1 ratio and stimulated with Staphylococcus enterotoxin B (SEB, 200 ng/mL, Sigma-Aldrich).

    Article Title: Therapeutic efficacy of a human papillomavirus type 16 E7 bacterial exotoxin fusion protein adjuvanted with CpG or GPI-0100 in a preclinical mouse model for HPV-associated disease
    Article Snippet: In vivo cytolytic activity Seven days after the last vaccination, mice (5/group) were injected intravenously under inhalation anesthesia with 10×106 CFSE-labeled naïve splenocytes that were pulsed with 0.5 μg/mL HPV16 E7(49–57) peptide or an irrelevant control peptide. .. E7-pulsed target cells were labeled with 10 μM CFSE (Vybrant Carboxyfluorescein diacetate succinimidyl ester (CFDA SE) Cell Tracer Kit, Thermo Fisher Scientific, Carlsbad, CA). .. Control peptide-pulsed cells were labeled with 0.66 μM CFSE.

    Co-Culture Assay:

    Article Title: Directional migration of adult hematopoeitic progenitors to C6 glioma in vitro
    Article Snippet: The phenotype and purity of the cell population was characterized by a FACScan flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) using anti-CD34/anti-CD133 fluorescein isothiocyanate-conjugated monoclonal antibodies. .. Prior to co-culture, the cells were stained with a fluorescent marker, the Vybrant CFDA SE Cell Tracer (V12883; Life Technologies, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    Staining:

    Article Title: Directional migration of adult hematopoeitic progenitors to C6 glioma in vitro
    Article Snippet: The phenotype and purity of the cell population was characterized by a FACScan flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) using anti-CD34/anti-CD133 fluorescein isothiocyanate-conjugated monoclonal antibodies. .. Prior to co-culture, the cells were stained with a fluorescent marker, the Vybrant CFDA SE Cell Tracer (V12883; Life Technologies, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    Article Title: Intratumoral delivery of mTORC2-deficient dendritic cells inhibits B16 melanoma growth by promoting CD8+ effector T cell responses
    Article Snippet: .. Control DC or Rictor−/− DC were stained with CFSE following the manufacturer's instructions (Vibrant CFDASE Cell Tracer Kit; Invitrogen, V12883) and 5 × 106 injected i.t. on day 7 post-tumor inoculation. .. After 3 d, tumors, spleens and inguinal lymph nodes were harvested and cells isolated and stained for analysis.

    Marker:

    Article Title: Directional migration of adult hematopoeitic progenitors to C6 glioma in vitro
    Article Snippet: The phenotype and purity of the cell population was characterized by a FACScan flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) using anti-CD34/anti-CD133 fluorescein isothiocyanate-conjugated monoclonal antibodies. .. Prior to co-culture, the cells were stained with a fluorescent marker, the Vybrant CFDA SE Cell Tracer (V12883; Life Technologies, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    Cell Culture:

    Article Title: Increased IGF-IEc expression and mechano-growth factor production in intestinal muscle of fibrostenotic Crohn's disease and smooth muscle hypertrophy
    Article Snippet: IGF-I receptor (cat. no. 3027) phosphorylation was measured in phospho-tyrosine (cat. no. 9411) (Cell Signaling Technologies) immunoblots as described previously ( ). .. Smooth muscle cell hypertrophy was measured in cells cultured on registered coverslip slides and microscope stage and loaded with Vybrant CFDA SE cell tracer (Life Technologies, Grand Island, NY). .. Medium was changed to Complete serum-free medium (Mediatech, Manassas, VA) on day 0 .

    Microscopy:

    Article Title: Increased IGF-IEc expression and mechano-growth factor production in intestinal muscle of fibrostenotic Crohn's disease and smooth muscle hypertrophy
    Article Snippet: IGF-I receptor (cat. no. 3027) phosphorylation was measured in phospho-tyrosine (cat. no. 9411) (Cell Signaling Technologies) immunoblots as described previously ( ). .. Smooth muscle cell hypertrophy was measured in cells cultured on registered coverslip slides and microscope stage and loaded with Vybrant CFDA SE cell tracer (Life Technologies, Grand Island, NY). .. Medium was changed to Complete serum-free medium (Mediatech, Manassas, VA) on day 0 .

    Proliferation Assay:

    Article Title: Expansion of myeloid-derived suppressor cells in patients with severe coronavirus disease (COVID-19)
    Article Snippet: The purity of sorted G-MDSC was > 90% as verified by flow cytometry. .. Proliferation assay PBMC and MDSC-depleted PBMC were labelled with CFDA-SE (Vibrant CFDA-SE cell tracer kit, Invitrogen) according with manufacturer’s instruction. .. CFDA-SE-stained PBMC, MDSC-depleted PBMC (depleted) or depleted cells seeded with purified polymorphonuclear-MDSC (PMN-MDSC) (1:5 ratio) were stimulated with Staphylococcus enterotoxin B (SEB, 800 ng/ml, Sigma Aldrich).

    Injection:

    Article Title: Intratumoral delivery of mTORC2-deficient dendritic cells inhibits B16 melanoma growth by promoting CD8+ effector T cell responses
    Article Snippet: .. Control DC or Rictor−/− DC were stained with CFSE following the manufacturer's instructions (Vibrant CFDASE Cell Tracer Kit; Invitrogen, V12883) and 5 × 106 injected i.t. on day 7 post-tumor inoculation. .. After 3 d, tumors, spleens and inguinal lymph nodes were harvested and cells isolated and stained for analysis.

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  • 95
    Thermo Fisher vybrant cfda se cell tracer
    Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of <t>Vybrant</t> <t>CFDA</t> SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P
    Vybrant Cfda Se Cell Tracer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vybrant cfda se cell tracer/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vybrant cfda se cell tracer - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of Vybrant CFDA SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Increased IGF-IEc expression and mechano-growth factor production in intestinal muscle of fibrostenotic Crohn's disease and smooth muscle hypertrophy

    doi: 10.1152/ajpgi.00414.2014

    Figure Lengend Snippet: Hypertrophy of human intestinal muscle cells is induced by synthetic MGF by an Erk-5-dependent mechanism. A : representative images of muscle cell hypertrophy induced by synthetic MGF but cell division induced by rhIGF-I. Hypertrophy was measured in the same groups of cells by confocal imaging of Vybrant CFDA SE cell tracer-loaded cells treated for 4 days with either synthetic MGF (100 nM) or rhIGF-I (100 nM). B : Z-stack analysis showing increased mean cell volume in cells treated with synthetic MGF (100 nM) but not rhIGF-I (100 nM). C : smooth muscle cell hypertrophy induced by synthetic MGF is inhibited by a selective Erk5 inhibitor. Hypertrophy was measured in cells incubated with synthetic MGF (100 nM) for 4 days in the presence or absence of BIX02188 (30 μM) from total cell protein per 10 4 cells. D : smooth muscle cell proliferation is induced by rhIGF-I but not synthetic MGF. Proliferation was measured by [ 3 H]thymidine incorporation into quiescent muscle cells incubated for 24 h with either synthetic MGF (1–100 nM) or rhIGF-I (1–100 nM). Results are means ± SE of 5–6 separate experiments. * P

    Article Snippet: Smooth muscle cell hypertrophy was measured in cells cultured on registered coverslip slides and microscope stage and loaded with Vybrant CFDA SE cell tracer (Life Technologies, Grand Island, NY).

    Techniques: Imaging, Incubation

    PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Daudi-induced Vδ2 T cell proliferation. Purified γδ T cells labeled with CFDA-SE were stimulated with Daudi cells and IL-2 in the presence of PMN-MDSC. After 5 days, Vδ2+ T cells proliferation was evaluated by flow cytometry. Representative histogram plots of one out of three independent experiments showing Vδ2 T cells proliferation in the indicated conditions.

    Journal: Frontiers in Immunology

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells

    doi: 10.3389/fimmu.2018.01271

    Figure Lengend Snippet: PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Daudi-induced Vδ2 T cell proliferation. Purified γδ T cells labeled with CFDA-SE were stimulated with Daudi cells and IL-2 in the presence of PMN-MDSC. After 5 days, Vδ2+ T cells proliferation was evaluated by flow cytometry. Representative histogram plots of one out of three independent experiments showing Vδ2 T cells proliferation in the indicated conditions.

    Article Snippet: In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction.

    Techniques: Derivative Assay, Purification, Labeling, Flow Cytometry, Cytometry

    PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Vδ2 T cell proliferation. γδ T cells or peripheral blood mononuclear cell (PBMC) labeled with CFDA-SE were stimulated with IPH in the presence of PMN-MDSC (1:1 ratio). After 5 days, Vδ2+ and CD3+ T cells proliferation was evaluated by flow cytometry. Representative gating strategy and histogram plots of one out of three independent experiments showing Vδ2 T cells (A) and CD3+ T cells (B) proliferation in the indicated conditions.

    Journal: Frontiers in Immunology

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells

    doi: 10.3389/fimmu.2018.01271

    Figure Lengend Snippet: PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Vδ2 T cell proliferation. γδ T cells or peripheral blood mononuclear cell (PBMC) labeled with CFDA-SE were stimulated with IPH in the presence of PMN-MDSC (1:1 ratio). After 5 days, Vδ2+ and CD3+ T cells proliferation was evaluated by flow cytometry. Representative gating strategy and histogram plots of one out of three independent experiments showing Vδ2 T cells (A) and CD3+ T cells (B) proliferation in the indicated conditions.

    Article Snippet: In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction.

    Techniques: Derivative Assay, Labeling, Flow Cytometry, Cytometry

    I.t.-delivered rictor −/− DC show similar migration to draining lymphoid tissue, but reduce the frequency of MDSC within the tumor. 5 × 10 6 CFSE-labeled control DC or Rictor −/− DC were injected i.t. on day 7 post-tumor

    Journal: Oncoimmunology

    Article Title: Intratumoral delivery of mTORC2-deficient dendritic cells inhibits B16 melanoma growth by promoting CD8+ effector T cell responses

    doi: 10.1080/2162402X.2016.1146841

    Figure Lengend Snippet: I.t.-delivered rictor −/− DC show similar migration to draining lymphoid tissue, but reduce the frequency of MDSC within the tumor. 5 × 10 6 CFSE-labeled control DC or Rictor −/− DC were injected i.t. on day 7 post-tumor

    Article Snippet: Control DC or Rictor−/− DC were stained with CFSE following the manufacturer's instructions (Vibrant CFDASE Cell Tracer Kit; Invitrogen, V12883) and 5 × 106 injected i.t. on day 7 post-tumor inoculation.

    Techniques: Migration, Labeling, Injection

    PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Daudi-induced Vδ2 T cell proliferation. Purified γδ T cells labeled with CFDA-SE were stimulated with Daudi cells and IL-2 in the presence of PMN-MDSC. After 5 days, Vδ2+ T cells proliferation was evaluated by flow cytometry. Representative histogram plots of one out of three independent experiments showing Vδ2 T cells proliferation in the indicated conditions.

    Journal: Frontiers in Immunology

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells

    doi: 10.3389/fimmu.2018.01271

    Figure Lengend Snippet: PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Daudi-induced Vδ2 T cell proliferation. Purified γδ T cells labeled with CFDA-SE were stimulated with Daudi cells and IL-2 in the presence of PMN-MDSC. After 5 days, Vδ2+ T cells proliferation was evaluated by flow cytometry. Representative histogram plots of one out of three independent experiments showing Vδ2 T cells proliferation in the indicated conditions.

    Article Snippet: In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction.

    Techniques: Derivative Assay, Purification, Labeling, Flow Cytometry, Cytometry

    PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Vδ2 T cell proliferation. γδ T cells or peripheral blood mononuclear cell (PBMC) labeled with CFDA-SE were stimulated with IPH in the presence of PMN-MDSC (1:1 ratio). After 5 days, Vδ2+ and CD3+ T cells proliferation was evaluated by flow cytometry. Representative gating strategy and histogram plots of one out of three independent experiments showing Vδ2 T cells (A) and CD3+ T cells (B) proliferation in the indicated conditions.

    Journal: Frontiers in Immunology

    Article Title: Myeloid-Derived Suppressor Cells Specifically Suppress IFN-γ Production and Antitumor Cytotoxic Activity of Vδ2 T Cells

    doi: 10.3389/fimmu.2018.01271

    Figure Lengend Snippet: PMN-myeloid-derived suppressor cells (MDSC) does not inhibit Vδ2 T cell proliferation. γδ T cells or peripheral blood mononuclear cell (PBMC) labeled with CFDA-SE were stimulated with IPH in the presence of PMN-MDSC (1:1 ratio). After 5 days, Vδ2+ and CD3+ T cells proliferation was evaluated by flow cytometry. Representative gating strategy and histogram plots of one out of three independent experiments showing Vδ2 T cells (A) and CD3+ T cells (B) proliferation in the indicated conditions.

    Article Snippet: In order to evaluate MDSC capacity to inhibit T cell proliferation, PBMC or purified γδ T cells were labeled with CFDA-SE (Vibrant CFDA SE cell tracer kit, Invitrogen) according with manufacturer’s instruction.

    Techniques: Derivative Assay, Labeling, Flow Cytometry, Cytometry