vu0529331  (Alomone Labs)


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    Alomone Labs vu0529331
    The effect of <t>VU0529331</t> on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
    Vu0529331, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vu0529331/product/Alomone Labs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    vu0529331 - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)"

    Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)

    Journal: bioRxiv

    doi: 10.1101/866202

    The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
    Figure Legend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.

    Techniques Used: Mutagenesis

    2) Product Images from "A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)"

    Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)

    Journal: bioRxiv

    doi: 10.1101/866202

    The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
    Figure Legend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.

    Techniques Used: Mutagenesis

    3) Product Images from "Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels"

    Article Title: Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels

    Journal: iScience

    doi: 10.1016/j.isci.2021.103018

    Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).
    Figure Legend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

    Techniques Used: Activity Assay, Activation Assay, Expressing

    4) Product Images from "A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)"

    Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)

    Journal: bioRxiv

    doi: 10.1101/866202

    The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
    Figure Legend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.

    Techniques Used: Mutagenesis

    5) Product Images from "Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels"

    Article Title: Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels

    Journal: iScience

    doi: 10.1016/j.isci.2021.103018

    Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).
    Figure Legend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

    Techniques Used: Activity Assay, Activation Assay, Expressing

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    Alomone Labs vu0529331
    The effect of <t>VU0529331</t> on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.
    Vu0529331, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vu0529331/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vu0529331 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

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    The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.

    Journal: bioRxiv

    Article Title: A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5)

    doi: 10.1101/866202

    Figure Lengend Snippet: The effect of VU0529331 on homotetrameric GIRK4 mutants. Oocytes expressed GIRK4 mutants and GIRK4 WT , with or without Gβγ. Cells were exposed to 4 concentrations of VU0529331 (1, 5, 20, and 60 μM). Only GIRK4 WT and GIRK4 G247R mutant responded to VU0529331. (A) I basal of GIRK4 WT and GIRK4 G247R . GIRK4 WT and GIRK4 G247R react similarly to VU0529331. (B) I βγ of GIRK4 WT and GIRK4 G247R . GIRK4 WT had larger current at 0 μM VU0529931, while GIRK4 G247R did not show any response to concentrations lower than 20 μM VU0529331. (C) Normalized I basal (I/I control ). GIRK4 WT and GIRK4 G247R were activated similarly by VU0529331. (D) I/I control of I βγ show that GIRK4 G247R was activated by VU0529331 stronger than GIRK4 WT , in relative terms. (E) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) without Gβγ. (F) I-V relationship of GIRK4 WT (top) and GIRK4 G247R (bottom) with Gβγ.

    Article Snippet: We are grateful to Alomone Labs for the generous gift of VU0529331 and to Mariam Ashkar for assistance in some of the experiments.

    Techniques: Mutagenesis

    Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

    Journal: iScience

    Article Title: Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels

    doi: 10.1016/j.isci.2021.103018

    Figure Lengend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

    Article Snippet: To measure GIRK2 response to VU0529331, the drug was diluted into HK24 solution to 2.5, 10 and 40 μM concentrations.

    Techniques: Activity Assay, Activation Assay, Expressing