Journal: Scientific Reports
Article Title: Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
doi: 10.1038/s41598-021-03623-y
Figure Lengend Snippet: In silico analysis of the oligonucleotide alignment for the RT-LAMP assay included in the study. ( A ) Genetic map of the complete genome of SARS-CoV-2 Wuhan-Hu-1. ( B ) Alignment of the RT-LAMP designed oligonucleotides with the sequences of human coronaviruses: SARS-CoV-2 coronavirus, SARS coronavirus Tor2, MERS-related coronavirus, hCov OC43, hCov HKU1 and hCov 229E. NoD No detected, NTC Not template control, MM master mix.
Article Snippet: The sequences of the designed oligonucleotides were compared with the sequences of human SARS-CoV-2 virus genomes and other coronaviruses described in the NCBI and GISAID: SARS coronavirus Tor2, complete genome (GBA: NC_004718.3); Middle East respiratory syndrome-related coronavirus isolate HCoV-EMC/2012 complete genome (GBA: NC_019843.3), Human coronavirus OC43 strain ATCC VR-759, complete genome (GBA: NC_006213.1); Human coronavirus HKU1, complete genome (GBA: NC_006577.2); Human coronavirus 229E, complete genome (GBA: NC_002645.1); and three genomes described in GISAID: hCoV-19/Peru/UN-INS-032/2020 (GISAID Accession: EPI_ISL_547948); hCoV-19/Peru/LIM-N18/2020 (GISAID Accession: EPI_ISL_490975); hCoV-19/Peru/LIM-UPCH 0157/2020 (GISAID Accession: EPI_ISL_568550).
Techniques: In Silico, RT Lamp Assay