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Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ ( a , c ) and PKC-δ−/− ( b , d ). a , b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c , d Three-dimensional (3D) images reconstructed from the confocal image stacks using <t>Voxblast.</t> (Field of view: 150 μm × 150 μm)
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Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ ( a , c ) and PKC-δ−/− ( b , d ). a , b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c , d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)

Journal: Arthritis Research & Therapy

Article Title: Protein kinase C delta null mice exhibit structural alterations in articular surface, intra-articular and subchondral compartments

doi: 10.1186/s13075-015-0720-4

Figure Lengend Snippet: Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ ( a , c ) and PKC-δ−/− ( b , d ). a , b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c , d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)

Article Snippet: After confocal image stacks were acquired, they were reconstructed into a three-dimensional image using VoxBlast software (VayTek Inc., Fairfield, Iowa, USA), and a maximum brightness image (MBI) generated using the computer software F900e, proprietary to the confocal microscope.

Techniques: