Journal: bioRxiv

Article Title: Eavesdropping roots: Fagus sylvatica detects belowground stress signals from conspecific and heterospecific ( Picea abies ) neighbors, triggering increased shoot VOC emissions

doi: 10.1101/2025.10.30.685342

Figure Lengend Snippet: Split-root design to analyze belowground inter- and intraspecific interactions between P. abies and F. sylvatica (mixed, n = 6, depicted) and between two individuals of F. sylvatica (mono, n = 6). Jasmonic acid (JA) solution (10 mM) was applied to the shoot of sender plants to simulate herbivory. Additionally, sender plants were labeled with 13 CO 2 and 15 NH 4 NO 3 (100 mL 0.01mM) the day after JA treatment. Plants were shielded aboveground from each other to allow biotic interactions solely via the root system in the shared soil compartment. Throughout the experimental period of ten days, VOC emissions, 13 CO 2 and H 2 O fluxes from the shoots and soil surface were monitored with a custom build automated flow-through gas system installed in two walk- in climate chambers. VOC emissions were analyzed using a proton-transfer time-of-flight mass spectrometer (PTR-TOF-MS). MFC = mass flow controller, MPV = multi position valve.

Article Snippet: The incoming and outgoing airstreams were analyzed in real-time for 13 CO 2 , H 2 O, and VOC concentrations using an infrared laser spectrometer (Delta Ray IRIS, Thermo Fisher Scientific), a non- dispersive infrared gas analyzer (LI-850, LI-COR Environmental, Bad Homburg, Germany), and a proton- transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS 4000 ultra, Ionicon Analytic, Innsbruck, Austria), respectively.

Techniques: Labeling, Mass Spectrometry

Journal: bioRxiv

Article Title: Eavesdropping roots: Fagus sylvatica detects belowground stress signals from conspecific and heterospecific ( Picea abies ) neighbors, triggering increased shoot VOC emissions

doi: 10.1101/2025.10.30.685342

Figure Lengend Snippet: Split-root design to analyze belowground inter- and intraspecific interactions between P. abies and F. sylvatica (mixed, n = 6, depicted) and between two individuals of F. sylvatica (mono, n = 6). Jasmonic acid (JA) solution (10 mM) was applied to the shoot of sender plants to simulate herbivory. Additionally, sender plants were labeled with 13 CO 2 and 15 NH 4 NO 3 (100 mL 0.01mM) the day after JA treatment. Plants were shielded aboveground from each other to allow biotic interactions solely via the root system in the shared soil compartment. Throughout the experimental period of ten days, VOC emissions, 13 CO 2 and H 2 O fluxes from the shoots and soil surface were monitored with a custom build automated flow-through gas system installed in two walk- in climate chambers. VOC emissions were analyzed using a proton-transfer time-of-flight mass spectrometer (PTR-TOF-MS). MFC = mass flow controller, MPV = multi position valve.

Article Snippet: The incoming and outgoing airstreams were analyzed in real-time for 13 CO 2 , H 2 O, and VOC concentrations using an infrared laser spectrometer (Delta Ray IRIS, Thermo Fisher Scientific), a non- dispersive infrared gas analyzer (LI-850, LI-COR Environmental, Bad Homburg, Germany), and a proton- transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS 4000 ultra, Ionicon Analytic, Innsbruck, Austria), respectively.

Techniques: Labeling, Mass Spectrometry

Journal: bioRxiv

Article Title: Eavesdropping roots: Fagus sylvatica detects belowground stress signals from conspecific and heterospecific ( Picea abies ) neighbors, triggering increased shoot VOC emissions

doi: 10.1101/2025.10.30.685342

Figure Lengend Snippet: Split-root design to analyze belowground inter- and intraspecific interactions between P. abies and F. sylvatica (mixed, n = 6, depicted) and between two individuals of F. sylvatica (mono, n = 6). Jasmonic acid (JA) solution (10 mM) was applied to the shoot of sender plants to simulate herbivory. Additionally, sender plants were labeled with 13 CO 2 and 15 NH 4 NO 3 (100 mL 0.01mM) the day after JA treatment. Plants were shielded aboveground from each other to allow biotic interactions solely via the root system in the shared soil compartment. Throughout the experimental period of ten days, VOC emissions, 13 CO 2 and H 2 O fluxes from the shoots and soil surface were monitored with a custom build automated flow-through gas system installed in two walk- in climate chambers. VOC emissions were analyzed using a proton-transfer time-of-flight mass spectrometer (PTR-TOF-MS). MFC = mass flow controller, MPV = multi position valve.

Article Snippet: The incoming and outgoing airstreams were analyzed in real-time for 13 CO 2 , H 2 O, and VOC concentrations using an infrared laser spectrometer (Delta Ray IRIS, Thermo Fisher Scientific), a non- dispersive infrared gas analyzer (LI-850, LI-COR Environmental, Bad Homburg, Germany), and a proton- transfer-reaction time-of-flight mass spectrometer (PTR-TOF-MS 4000 ultra, Ionicon Analytic, Innsbruck, Austria), respectively.

Techniques: Labeling, Mass Spectrometry