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Roche viral ribonucleic acid rna
Viral Ribonucleic Acid Rna, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 3 article reviews
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viral ribonucleic acid rna - by Bioz Stars, 2020-04
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Reverse Transcription Polymerase Chain Reaction:

Article Title: Spatial Analyses of Oral Polio Vaccine Transmission in an Community Vaccinated With Inactivated Polio Vaccine
Article Snippet: Viral ribonucleic acid (RNA) was extracted from frozen stool samples utilizing the MagNA Lyser (Roche) and KingFisher Duo Prime (Fisher Scientific), using the bacteriophage MS2 as an internal control for extraction efficiency. .. Viral RNA then underwent quantitative, reverse-transcription polymerase chain reaction (rt-QPCR) in order to detect and quantify any Sabin OPV present in the samples.

Real-time Polymerase Chain Reaction:

Article Title: Spatial Analyses of Oral Polio Vaccine Transmission in an Community Vaccinated With Inactivated Polio Vaccine
Article Snippet: Viral ribonucleic acid (RNA) was extracted from frozen stool samples utilizing the MagNA Lyser (Roche) and KingFisher Duo Prime (Fisher Scientific), using the bacteriophage MS2 as an internal control for extraction efficiency. .. The probes and primers were adopted and adapted from Kilpatrick et al. [ ] and the Centers for Disease Control protocol for polio quantitative-PCR.

Quantitative RT-PCR:

Article Title: Spatial Analyses of Oral Polio Vaccine Transmission in an Community Vaccinated With Inactivated Polio Vaccine
Article Snippet: Viral ribonucleic acid (RNA) was extracted from frozen stool samples utilizing the MagNA Lyser (Roche) and KingFisher Duo Prime (Fisher Scientific), using the bacteriophage MS2 as an internal control for extraction efficiency. .. Viral RNA then underwent quantitative, reverse-transcription polymerase chain reaction (rt-QPCR) in order to detect and quantify any Sabin OPV present in the samples.

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  • 81
    Roche quantitative hiv 1 rna assay
    Sensitivity of the LCx <t>HIV-1/2</t> qualitative <t>RNA</t> assay with HIV-1 subtype A to F clones. Cloned fragments containing a region of the pol genes from HIV-1 subtypes A to F were transcribed in vitro. The transcripts were quantitated by hyperchromicity, diluted to the indicated concentrations, and tested by the HIV-1/2 qualitative RNA assay. An S/CO of > 1 is considered a positive result. rxn, reaction.
    Quantitative Hiv 1 Rna Assay, supplied by Roche, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative hiv 1 rna assay/product/Roche
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    87
    Roche viral kinetics plasma hcv rna load
    NKG2D expression on CD56+CD3+ lymphocytes predicts treatment responses to PEG-IFN/ ribavirin therapy. PBMCs were collected and analyzed by flow cytometry for NKG2D expression on CD56+CD3+ lymphocytes before treatment. Plasma <t>HCV-RNA</t> levels were measured regularly to monitor viral kinetics during treatment. (A, left) Correlation of decreased plasma HCV RNA (IU/ml) from week 0 to week 4 and NKG2D expression on CD56+CD3+ lymphocytes is shown. (A, right) NKG2D expression was analyzed to compare RVR (n = 8) and non-RVR (n = 22) cases. (B) NKG2D expression on CD56+CD3+ lymphocytes was analyzed to (i) compare SVR (n = 17) and non-SVR (n = 13) cases; (ii) SVR (n = 4), PR (n = 9), and NR (n = 4) in genotype 1 infection; (iii) ROC analysis to predict SVR by NKG2D expression on CD56+CD3+ lymphocytes. (C) Pre-treatment peripheral CD56+CD3+ lymphocytes were stratified into CD4+CD8-, CD8+CD4- and CD4-CD8- double negative (DN) subpopulations. NKG2D expression on corresponding subpopulations were determined. Statistics of NKG2D expression were compared between SVR (n = 17) and non-SVR (n = 13) cases, including both genotypes. Data show mean ± SD. *P
    Viral Kinetics Plasma Hcv Rna Load, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 1 article reviews
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    86
    Roche quantitative real time reverse transcription pcr viral rna
    Replication of influenza A in duck and chicken cell cultures. Chicken cells (white bars) and duck cells (black bars) were infected with a range of viruses for 20–48 h and virus production measured by real-time <t>PCR</t> and titration of virus in culture supernatants. ( a ) Duck and chicken lung cells infected with LPAI H2N3 at an MOI of 1.0 for 24 h and virus output measured by detection of viral M gene <t>RNA</t> in supernatant by quantitative reverse transcription PCR. ( b ) Duck and chicken embryo cells infected with HPAI H5N1 50-92 or H5N1 tyTy05 for 20 h at an MOI of 1.0 and virus production quantified by viral M gene RNA PCR. Significant difference in M gene copy number between species following H5N1 50-92 ( P
    Quantitative Real Time Reverse Transcription Pcr Viral Rna, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time reverse transcription pcr viral rna/product/Roche
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    94
    Roche hiv 1 rna
    <t>HIV-1</t> <t>RNA</t> quantification in culture supernatants using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay. Culture supernatants containing a range of HIV-1 RNAs, induced following HDACi treatments, anti-CD3/CD28 MAb treatment, givinostat plus anti-CD3/CD28 MAb treatment, or unexposed cells ( n = 38) were assessed for HIV-1 RNA using the Roche TaqMan assay (Roche) and Abbott RealTime HIV-1 assay (Abbott). HIV-1 RNA quantification was performed using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay in VOA culture supernatants obtained following anti-CD3/CD28 MAb treatment (A) or treatment with vorinostat (B), romidepsin (C), panobinostat (D), givinostat (E), belinostat (F), or givinostat plus anti-CD3/CD28 MAbs (G). Panel H shows the total results. (I) Correlation between HIV-1 RNA quantification using the Roche TaqMan assay and Abbott RealTime HIV-1. Histograms correspond to the mean, and red error bars correspond to the SEM. NS, not significant. Statistical significance ( P values) was obtained using Wilcoxon's matched-pair two-tailed signed rank test (A to H) or Spearman's rank correlations (I).
    Hiv 1 Rna, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 rna/product/Roche
    Average 94 stars, based on 4 article reviews
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    Image Search Results


    Sensitivity of the LCx HIV-1/2 qualitative RNA assay with HIV-1 subtype A to F clones. Cloned fragments containing a region of the pol genes from HIV-1 subtypes A to F were transcribed in vitro. The transcripts were quantitated by hyperchromicity, diluted to the indicated concentrations, and tested by the HIV-1/2 qualitative RNA assay. An S/CO of > 1 is considered a positive result. rxn, reaction.

    Journal: Journal of Clinical Microbiology

    Article Title: Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2

    doi:

    Figure Lengend Snippet: Sensitivity of the LCx HIV-1/2 qualitative RNA assay with HIV-1 subtype A to F clones. Cloned fragments containing a region of the pol genes from HIV-1 subtypes A to F were transcribed in vitro. The transcripts were quantitated by hyperchromicity, diluted to the indicated concentrations, and tested by the HIV-1/2 qualitative RNA assay. An S/CO of > 1 is considered a positive result. rxn, reaction.

    Article Snippet: For most of the seroconversion panels tested, the viral loads of the samples, measured by a quantitative HIV-1 RNA assay (Amplicor Monitor, version 1.0; Roche Diagnostic Systems, Somerville, N.J.), which has a low limit of detection of 400 copies per ml, was provided by the vendors.

    Techniques: Clone Assay, In Vitro

    NKG2D expression on CD56+CD3+ lymphocytes predicts treatment responses to PEG-IFN/ ribavirin therapy. PBMCs were collected and analyzed by flow cytometry for NKG2D expression on CD56+CD3+ lymphocytes before treatment. Plasma HCV-RNA levels were measured regularly to monitor viral kinetics during treatment. (A, left) Correlation of decreased plasma HCV RNA (IU/ml) from week 0 to week 4 and NKG2D expression on CD56+CD3+ lymphocytes is shown. (A, right) NKG2D expression was analyzed to compare RVR (n = 8) and non-RVR (n = 22) cases. (B) NKG2D expression on CD56+CD3+ lymphocytes was analyzed to (i) compare SVR (n = 17) and non-SVR (n = 13) cases; (ii) SVR (n = 4), PR (n = 9), and NR (n = 4) in genotype 1 infection; (iii) ROC analysis to predict SVR by NKG2D expression on CD56+CD3+ lymphocytes. (C) Pre-treatment peripheral CD56+CD3+ lymphocytes were stratified into CD4+CD8-, CD8+CD4- and CD4-CD8- double negative (DN) subpopulations. NKG2D expression on corresponding subpopulations were determined. Statistics of NKG2D expression were compared between SVR (n = 17) and non-SVR (n = 13) cases, including both genotypes. Data show mean ± SD. *P

    Journal: PLoS ONE

    Article Title: Genotype-Associated Differential NKG2D Expression on CD56+CD3+ Lymphocytes Predicts Response to Pegylated-Interferon/ Ribavirin Therapy in Chronic Hepatitis C

    doi: 10.1371/journal.pone.0125664

    Figure Lengend Snippet: NKG2D expression on CD56+CD3+ lymphocytes predicts treatment responses to PEG-IFN/ ribavirin therapy. PBMCs were collected and analyzed by flow cytometry for NKG2D expression on CD56+CD3+ lymphocytes before treatment. Plasma HCV-RNA levels were measured regularly to monitor viral kinetics during treatment. (A, left) Correlation of decreased plasma HCV RNA (IU/ml) from week 0 to week 4 and NKG2D expression on CD56+CD3+ lymphocytes is shown. (A, right) NKG2D expression was analyzed to compare RVR (n = 8) and non-RVR (n = 22) cases. (B) NKG2D expression on CD56+CD3+ lymphocytes was analyzed to (i) compare SVR (n = 17) and non-SVR (n = 13) cases; (ii) SVR (n = 4), PR (n = 9), and NR (n = 4) in genotype 1 infection; (iii) ROC analysis to predict SVR by NKG2D expression on CD56+CD3+ lymphocytes. (C) Pre-treatment peripheral CD56+CD3+ lymphocytes were stratified into CD4+CD8-, CD8+CD4- and CD4-CD8- double negative (DN) subpopulations. NKG2D expression on corresponding subpopulations were determined. Statistics of NKG2D expression were compared between SVR (n = 17) and non-SVR (n = 13) cases, including both genotypes. Data show mean ± SD. *P

    Article Snippet: Viral Kinetics Plasma HCV RNA load was measured using COBAS TaqMan HCV test, with a lower limit of detection of 15 IU/ml (Roche Diagnostics Co, Ltd, Tokyo, Japan).

    Techniques: Expressing, Flow Cytometry, Cytometry, Infection

    NKG2D expression on CD56+CD3+ lymphocytes has no significant correlation to age, HCV-RNA levels or liver fibrotic degree. PBMCs were collected and analyzed by flow cytometry for expression of NKG2D on CD56+CD3+ lymphocytes. Statistics of data including age, HCV-RNA, peripheral platelet count, and serum type IV collagen 7s levels were analyzed for correlation. Please refer to supporting S1 Table for detailed values.

    Journal: PLoS ONE

    Article Title: Genotype-Associated Differential NKG2D Expression on CD56+CD3+ Lymphocytes Predicts Response to Pegylated-Interferon/ Ribavirin Therapy in Chronic Hepatitis C

    doi: 10.1371/journal.pone.0125664

    Figure Lengend Snippet: NKG2D expression on CD56+CD3+ lymphocytes has no significant correlation to age, HCV-RNA levels or liver fibrotic degree. PBMCs were collected and analyzed by flow cytometry for expression of NKG2D on CD56+CD3+ lymphocytes. Statistics of data including age, HCV-RNA, peripheral platelet count, and serum type IV collagen 7s levels were analyzed for correlation. Please refer to supporting S1 Table for detailed values.

    Article Snippet: Viral Kinetics Plasma HCV RNA load was measured using COBAS TaqMan HCV test, with a lower limit of detection of 15 IU/ml (Roche Diagnostics Co, Ltd, Tokyo, Japan).

    Techniques: Expressing, Flow Cytometry, Cytometry

    Replication of influenza A in duck and chicken cell cultures. Chicken cells (white bars) and duck cells (black bars) were infected with a range of viruses for 20–48 h and virus production measured by real-time PCR and titration of virus in culture supernatants. ( a ) Duck and chicken lung cells infected with LPAI H2N3 at an MOI of 1.0 for 24 h and virus output measured by detection of viral M gene RNA in supernatant by quantitative reverse transcription PCR. ( b ) Duck and chicken embryo cells infected with HPAI H5N1 50-92 or H5N1 tyTy05 for 20 h at an MOI of 1.0 and virus production quantified by viral M gene RNA PCR. Significant difference in M gene copy number between species following H5N1 50-92 ( P

    Journal: Immunology and Cell Biology

    Article Title: Rapid death of duck cells infected with influenza: a potential mechanism for host resistance to H5N1

    doi: 10.1038/icb.2011.17

    Figure Lengend Snippet: Replication of influenza A in duck and chicken cell cultures. Chicken cells (white bars) and duck cells (black bars) were infected with a range of viruses for 20–48 h and virus production measured by real-time PCR and titration of virus in culture supernatants. ( a ) Duck and chicken lung cells infected with LPAI H2N3 at an MOI of 1.0 for 24 h and virus output measured by detection of viral M gene RNA in supernatant by quantitative reverse transcription PCR. ( b ) Duck and chicken embryo cells infected with HPAI H5N1 50-92 or H5N1 tyTy05 for 20 h at an MOI of 1.0 and virus production quantified by viral M gene RNA PCR. Significant difference in M gene copy number between species following H5N1 50-92 ( P

    Article Snippet: Quantification of virus production using quantitative real-time reverse transcription PCR Viral RNA from culture supernatants was extracted using Hipure viral RNA kit (Roche Diagnostics Ltd., Burgess Hill, UK) or QIAamp Viral RNA Mini Kit (Qiagen, Crawley, UK).

    Techniques: Infection, Real-time Polymerase Chain Reaction, Titration, Polymerase Chain Reaction

    HIV-1 RNA quantification in culture supernatants using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay. Culture supernatants containing a range of HIV-1 RNAs, induced following HDACi treatments, anti-CD3/CD28 MAb treatment, givinostat plus anti-CD3/CD28 MAb treatment, or unexposed cells ( n = 38) were assessed for HIV-1 RNA using the Roche TaqMan assay (Roche) and Abbott RealTime HIV-1 assay (Abbott). HIV-1 RNA quantification was performed using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay in VOA culture supernatants obtained following anti-CD3/CD28 MAb treatment (A) or treatment with vorinostat (B), romidepsin (C), panobinostat (D), givinostat (E), belinostat (F), or givinostat plus anti-CD3/CD28 MAbs (G). Panel H shows the total results. (I) Correlation between HIV-1 RNA quantification using the Roche TaqMan assay and Abbott RealTime HIV-1. Histograms correspond to the mean, and red error bars correspond to the SEM. NS, not significant. Statistical significance ( P values) was obtained using Wilcoxon's matched-pair two-tailed signed rank test (A to H) or Spearman's rank correlations (I).

    Journal: Journal of Virology

    Article Title: In Vitro Reactivation of Replication-Competent and Infectious HIV-1 by Histone Deacetylase Inhibitors

    doi: 10.1128/JVI.02359-15

    Figure Lengend Snippet: HIV-1 RNA quantification in culture supernatants using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay. Culture supernatants containing a range of HIV-1 RNAs, induced following HDACi treatments, anti-CD3/CD28 MAb treatment, givinostat plus anti-CD3/CD28 MAb treatment, or unexposed cells ( n = 38) were assessed for HIV-1 RNA using the Roche TaqMan assay (Roche) and Abbott RealTime HIV-1 assay (Abbott). HIV-1 RNA quantification was performed using Roche TaqMan assay versus the Abbott RealTime HIV-1 assay in VOA culture supernatants obtained following anti-CD3/CD28 MAb treatment (A) or treatment with vorinostat (B), romidepsin (C), panobinostat (D), givinostat (E), belinostat (F), or givinostat plus anti-CD3/CD28 MAbs (G). Panel H shows the total results. (I) Correlation between HIV-1 RNA quantification using the Roche TaqMan assay and Abbott RealTime HIV-1. Histograms correspond to the mean, and red error bars correspond to the SEM. NS, not significant. Statistical significance ( P values) was obtained using Wilcoxon's matched-pair two-tailed signed rank test (A to H) or Spearman's rank correlations (I).

    Article Snippet: With regard to the levels of HIV-1 RNA and P24 measured in the presence of the different HDACis, vorinostat and romidepsin were less efficient at inducing HIV-1 RNA and P24 production than panobinostat, givinostat, and belinostat, which induced levels comparable to anti-CD3/CD28 MAb stimulation ( and ).

    Techniques: TaqMan Assay, Two Tailed Test

    Prolonged/repeated exposure of resting memory CD4 T cells to HDACis is the primary mechanism responsible for efficient induction of HIV-1 replication by HDACi. (A) Levels of HIV-1 RNA (copies per milliliter) induced following givinostat treatment ( n = 4; 5 replicates). (B) Levels of P24 (ECL units per milliliter) induced following givinostat treatment ( n = 4; 5 replicates). Subjects were color coded, and each color corresponds to a subject. US, unstimulated or unexposed. Histograms correspond to the mean, and red error bars correspond to the SEM. Red asterisks indicate statistical significance ( P

    Journal: Journal of Virology

    Article Title: In Vitro Reactivation of Replication-Competent and Infectious HIV-1 by Histone Deacetylase Inhibitors

    doi: 10.1128/JVI.02359-15

    Figure Lengend Snippet: Prolonged/repeated exposure of resting memory CD4 T cells to HDACis is the primary mechanism responsible for efficient induction of HIV-1 replication by HDACi. (A) Levels of HIV-1 RNA (copies per milliliter) induced following givinostat treatment ( n = 4; 5 replicates). (B) Levels of P24 (ECL units per milliliter) induced following givinostat treatment ( n = 4; 5 replicates). Subjects were color coded, and each color corresponds to a subject. US, unstimulated or unexposed. Histograms correspond to the mean, and red error bars correspond to the SEM. Red asterisks indicate statistical significance ( P

    Article Snippet: With regard to the levels of HIV-1 RNA and P24 measured in the presence of the different HDACis, vorinostat and romidepsin were less efficient at inducing HIV-1 RNA and P24 production than panobinostat, givinostat, and belinostat, which induced levels comparable to anti-CD3/CD28 MAb stimulation ( and ).

    Techniques:

    Correlation between P24 and HIV-1 RNA levels detected in culture supernatants following the viral outgrowth assay. Correlation between P24 and HIV-1 RNA levels ( n = 50; 10 subjects, 5 replicates per condition) was obtained following anti-CD3/CD28 MAb treatment (A) or treatment with vorinostat (B), romidepsin (C), panobinostat (D), givinostat (E), or belinostat (F). Each circle corresponds to one replicate, and each color corresponds to one HIV-1-infected subject. Dotted lines correspond to the limit of detections. Statistical significance ( P values) was obtained using Spearman's rank correlations.

    Journal: Journal of Virology

    Article Title: In Vitro Reactivation of Replication-Competent and Infectious HIV-1 by Histone Deacetylase Inhibitors

    doi: 10.1128/JVI.02359-15

    Figure Lengend Snippet: Correlation between P24 and HIV-1 RNA levels detected in culture supernatants following the viral outgrowth assay. Correlation between P24 and HIV-1 RNA levels ( n = 50; 10 subjects, 5 replicates per condition) was obtained following anti-CD3/CD28 MAb treatment (A) or treatment with vorinostat (B), romidepsin (C), panobinostat (D), givinostat (E), or belinostat (F). Each circle corresponds to one replicate, and each color corresponds to one HIV-1-infected subject. Dotted lines correspond to the limit of detections. Statistical significance ( P values) was obtained using Spearman's rank correlations.

    Article Snippet: With regard to the levels of HIV-1 RNA and P24 measured in the presence of the different HDACis, vorinostat and romidepsin were less efficient at inducing HIV-1 RNA and P24 production than panobinostat, givinostat, and belinostat, which induced levels comparable to anti-CD3/CD28 MAb stimulation ( and ).

    Techniques: Viral Outgrowth Assay, Infection

    HDACis efficiently reactivate HIV-1 replication from latently infected resting memory CD4 T cells isolated from long-term-treated HIV-1-infected subjects. (A) Schematic representation of the modified VOA. (B) Proportion of responders to HDACis treatment based on the detection of HIV-1 RNA ( n = 10; 5 replicates per condition). Individuals having at least one replicate with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as “responders” for the condition tested. (C) Proportion of responders to HDACi treatment based on the detection of P24 ( n = 10; 5 replicates per condition). Individuals having at least one replicate with detectable P24 (≥1 ECL unit/ml) are indicated as “responders” for the condition tested. (D) Proportion of HIV-1 RNA-positive wells induced following HDACis treatment ( n = 10; 5 replicates per condition). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated to as HIV-1 RNA positive for the condition tested. (E) Proportion of P24-positive wells induced following HDACis treatment ( n = 10; 5 replicates per condition). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24 positive for the condition tested. (F) Levels of HIV-1 RNA copies per milliliter induced following HDACis treatment ( n = 10; 5 replicates per condition). (G) Levels of P24 (ECL units per milliliter) induced following HDACis treatment ( n = 10; 5 replicates per condition). (H and I) Frequencies of inducible replication-competent virus as measured by replication-competent (RNA) units per million (RUPM) (H) or as measured by infectious units per million (IUPM) (I). (J) Correlation between P24 and HIV-1 RNA levels ( n = 350; 10 subjects, 7 conditions, 5 replicates per condition). Panels B to G and J were generated using the 5 replicates of the lowest dilution of cells (5 × 10 5 cells) of all conditions by modified VOA. Subjects were color coded, and each color corresponds to a subject (F to J). Histograms correspond to the mean (B to I), and red error bars correspond to the standard error of the mean (SEM) (F to I). Blue lines correspond to the median (F to I). Red asterisks indicate statistical significance compared to unstimulated or unexposed (US) ( P

    Journal: Journal of Virology

    Article Title: In Vitro Reactivation of Replication-Competent and Infectious HIV-1 by Histone Deacetylase Inhibitors

    doi: 10.1128/JVI.02359-15

    Figure Lengend Snippet: HDACis efficiently reactivate HIV-1 replication from latently infected resting memory CD4 T cells isolated from long-term-treated HIV-1-infected subjects. (A) Schematic representation of the modified VOA. (B) Proportion of responders to HDACis treatment based on the detection of HIV-1 RNA ( n = 10; 5 replicates per condition). Individuals having at least one replicate with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as “responders” for the condition tested. (C) Proportion of responders to HDACi treatment based on the detection of P24 ( n = 10; 5 replicates per condition). Individuals having at least one replicate with detectable P24 (≥1 ECL unit/ml) are indicated as “responders” for the condition tested. (D) Proportion of HIV-1 RNA-positive wells induced following HDACis treatment ( n = 10; 5 replicates per condition). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated to as HIV-1 RNA positive for the condition tested. (E) Proportion of P24-positive wells induced following HDACis treatment ( n = 10; 5 replicates per condition). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24 positive for the condition tested. (F) Levels of HIV-1 RNA copies per milliliter induced following HDACis treatment ( n = 10; 5 replicates per condition). (G) Levels of P24 (ECL units per milliliter) induced following HDACis treatment ( n = 10; 5 replicates per condition). (H and I) Frequencies of inducible replication-competent virus as measured by replication-competent (RNA) units per million (RUPM) (H) or as measured by infectious units per million (IUPM) (I). (J) Correlation between P24 and HIV-1 RNA levels ( n = 350; 10 subjects, 7 conditions, 5 replicates per condition). Panels B to G and J were generated using the 5 replicates of the lowest dilution of cells (5 × 10 5 cells) of all conditions by modified VOA. Subjects were color coded, and each color corresponds to a subject (F to J). Histograms correspond to the mean (B to I), and red error bars correspond to the standard error of the mean (SEM) (F to I). Blue lines correspond to the median (F to I). Red asterisks indicate statistical significance compared to unstimulated or unexposed (US) ( P

    Article Snippet: With regard to the levels of HIV-1 RNA and P24 measured in the presence of the different HDACis, vorinostat and romidepsin were less efficient at inducing HIV-1 RNA and P24 production than panobinostat, givinostat, and belinostat, which induced levels comparable to anti-CD3/CD28 MAb stimulation ( and ).

    Techniques: Infection, Isolation, Modification, Generated

    Assessment of potential synergistic effect between givinostat treatment and TCR stimulation or PKC agonist on the reactivation of HIV-1 replication. (A) Proportion of HIV-1 RNA-positive wells following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as HIV-1 RNA-positive wells for the condition tested. (B) Proportion of P24-positive wells following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24 positive for the condition tested. (C) HIV-1 RNA (copies per milliliter) induced following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). (D) Levels of P24 (ECL units per milliliter) induced following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). (E) Proportion of HIV-1 RNA-positive wells following treatment with anti-CD3/CD28 MAbs or givinostat (Givi) and/or bryostatin (Bryo) ( n = 3). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as HIV-1 RNA-positive wells for the condition tested. US, unstimulated. (F) Proportion of P24-positive wells following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24-positive wells for the condition tested. (G) Levels of HIV-1 RNA copies per milliliter induced following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). (H) Levels of HIV-1 P24 (ECL units per milliliter) induced following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). Subjects were color coded, and each color corresponds to a subject (C and D and G and H). Red error bars correspond to means ± SEM. Red asterisks indicate statistical significance ( P

    Journal: Journal of Virology

    Article Title: In Vitro Reactivation of Replication-Competent and Infectious HIV-1 by Histone Deacetylase Inhibitors

    doi: 10.1128/JVI.02359-15

    Figure Lengend Snippet: Assessment of potential synergistic effect between givinostat treatment and TCR stimulation or PKC agonist on the reactivation of HIV-1 replication. (A) Proportion of HIV-1 RNA-positive wells following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as HIV-1 RNA-positive wells for the condition tested. (B) Proportion of P24-positive wells following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24 positive for the condition tested. (C) HIV-1 RNA (copies per milliliter) induced following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). (D) Levels of P24 (ECL units per milliliter) induced following treatment with givinostat and/or anti-CD3/CD28 MAbs ( n = 4). (E) Proportion of HIV-1 RNA-positive wells following treatment with anti-CD3/CD28 MAbs or givinostat (Givi) and/or bryostatin (Bryo) ( n = 3). Wells with detectable HIV-1 RNA (≥200 HIV-1 RNA copies/ml) are indicated as HIV-1 RNA-positive wells for the condition tested. US, unstimulated. (F) Proportion of P24-positive wells following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). Wells with detectable P24 (≥1 ECL unit/ml) are indicated as P24-positive wells for the condition tested. (G) Levels of HIV-1 RNA copies per milliliter induced following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). (H) Levels of HIV-1 P24 (ECL units per milliliter) induced following treatment with anti-CD3/CD28 MAbs or givinostat and/or bryostatin ( n = 3). Subjects were color coded, and each color corresponds to a subject (C and D and G and H). Red error bars correspond to means ± SEM. Red asterisks indicate statistical significance ( P

    Article Snippet: With regard to the levels of HIV-1 RNA and P24 measured in the presence of the different HDACis, vorinostat and romidepsin were less efficient at inducing HIV-1 RNA and P24 production than panobinostat, givinostat, and belinostat, which induced levels comparable to anti-CD3/CD28 MAb stimulation ( and ).

    Techniques: