viia7 system  (Thermo Fisher)


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    Name:
    ViiA 7 Dx Instrument
    Description:
    The ViiA 7 Dx Instrument puts high productivity qPCR at your fingertips With the new OptiFlex optical system and 4 interchangeable block formats from 96 well to 384 well to Micro Fludic Array Card the ViiA 7 Dx Instrument is available for in vitro diagnostics use Key product features • Proven Applied Biosystems reliability and accuracy • Using with Micro Fluidic Array Cards provides an integrated workflow and faster results • Easy to use intuitive software responsive touch screen effortless block exchange without the need for any tools ProductivityIdeal for performing medium to high throughput real time PCR the ViiA 7 Dx Instrument enhances your lab s productivity • Quick block changes Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers• Easy touch screen interface Instrument touch screen provides one touch protocols for fast and easy assay setup for a broad range of applications• Peace of mind Data recovery capability for 100 runsPerformanceThe ViiA 7 Dx Instrument features the new OptiFlex System which provides enhanced fluorescence detection • Maximum multiplexing Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities• Flexible data collection Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage• Precise quantification Detect as small as 1 5 fold changes in target quantities in singleplex reactionsViiA 7 Dx Software drives value in a number of ways offering • Intuitive interface and innovative design • Convenient walk away operation • Improved data analysis • Fully compatible with high throughput environments Note The ViiA 7 Dx Instrument and consumables are for distribution and use in specific European countries only Not for use in the USA
    Catalog Number:
    4453539
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Real Time PCR (qPCR)|Real-Time PCR Instruments, Software & Calibration
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher viia7 system
    The ViiA 7 Dx Instrument puts high productivity qPCR at your fingertips With the new OptiFlex optical system and 4 interchangeable block formats from 96 well to 384 well to Micro Fludic Array Card the ViiA 7 Dx Instrument is available for in vitro diagnostics use Key product features • Proven Applied Biosystems reliability and accuracy • Using with Micro Fluidic Array Cards provides an integrated workflow and faster results • Easy to use intuitive software responsive touch screen effortless block exchange without the need for any tools ProductivityIdeal for performing medium to high throughput real time PCR the ViiA 7 Dx Instrument enhances your lab s productivity • Quick block changes Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers• Easy touch screen interface Instrument touch screen provides one touch protocols for fast and easy assay setup for a broad range of applications• Peace of mind Data recovery capability for 100 runsPerformanceThe ViiA 7 Dx Instrument features the new OptiFlex System which provides enhanced fluorescence detection • Maximum multiplexing Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities• Flexible data collection Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage• Precise quantification Detect as small as 1 5 fold changes in target quantities in singleplex reactionsViiA 7 Dx Software drives value in a number of ways offering • Intuitive interface and innovative design • Convenient walk away operation • Improved data analysis • Fully compatible with high throughput environments Note The ViiA 7 Dx Instrument and consumables are for distribution and use in specific European countries only Not for use in the USA
    https://www.bioz.com/result/viia7 system/product/Thermo Fisher
    Average 99 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    viia7 system - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: The role of miR-146a in dorsal root ganglia neurons of experimental diabetic peripheral neuropathy
    Article Snippet: .. Using the SYBR Green real-time PCR method ( , ), quantitative PCR was performed on an ViiA7 Instrument (Applied Biosystems, Foster City, CA) by means of three-stage program parameters provided by the manufacturer, as follows; 2 min at 50 °C, 10 min at 95 °C, and then 40 cycles of 15 s at 95 °C and 1 min at 60 °C. .. Specificity of the produced amplification product was confirmed by examination of dissociation reaction plots.

    Fluorescence:

    Article Title: Transcriptome Analysis Identifies Immune Markers Related to Visceral Leishmaniasis Establishment in the Experimental Model of BALB/c Mice
    Article Snippet: .. Gene expression was determined by measuring the fluorescence signals in real time with the use of ViiA™7 Real Time PCR System (Applied Biosystems) and critical threshold values were generated using the ViiA™7 Software Version 1.2.1 (Applied Biosystems). .. HPRT was used as an internal control to calculate fold changes in target genes in spleen biopsy samples obtained from infected mice that have been previously vaccinated with CPA160−189 -loaded p8-PLGA nanoparticles and non-vaccinated infected mice, by use of 2−ΔΔCT method.

    Isolation:

    Article Title: B1a B cells require autophagy for metabolic homeostasis and self-renewal
    Article Snippet: .. qRT-PCR RNA was isolated using the RNeasy Micro kit (Qiagen) and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher). qRT-PCR was performed using Taqman Gene Expression Master Mix on a ViiA 7 instrument (Thermo Fisher). .. The PCR probes used are listed in Table S1.

    Quantitative RT-PCR:

    Article Title: B1a B cells require autophagy for metabolic homeostasis and self-renewal
    Article Snippet: .. qRT-PCR RNA was isolated using the RNeasy Micro kit (Qiagen) and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher). qRT-PCR was performed using Taqman Gene Expression Master Mix on a ViiA 7 instrument (Thermo Fisher). .. The PCR probes used are listed in Table S1.

    Real-time Polymerase Chain Reaction:

    Article Title: The role of miR-146a in dorsal root ganglia neurons of experimental diabetic peripheral neuropathy
    Article Snippet: .. Using the SYBR Green real-time PCR method ( , ), quantitative PCR was performed on an ViiA7 Instrument (Applied Biosystems, Foster City, CA) by means of three-stage program parameters provided by the manufacturer, as follows; 2 min at 50 °C, 10 min at 95 °C, and then 40 cycles of 15 s at 95 °C and 1 min at 60 °C. .. Specificity of the produced amplification product was confirmed by examination of dissociation reaction plots.

    Article Title: Transcriptome Analysis Identifies Immune Markers Related to Visceral Leishmaniasis Establishment in the Experimental Model of BALB/c Mice
    Article Snippet: .. Gene expression was determined by measuring the fluorescence signals in real time with the use of ViiA™7 Real Time PCR System (Applied Biosystems) and critical threshold values were generated using the ViiA™7 Software Version 1.2.1 (Applied Biosystems). .. HPRT was used as an internal control to calculate fold changes in target genes in spleen biopsy samples obtained from infected mice that have been previously vaccinated with CPA160−189 -loaded p8-PLGA nanoparticles and non-vaccinated infected mice, by use of 2−ΔΔCT method.

    Generated:

    Article Title: Transcriptome Analysis Identifies Immune Markers Related to Visceral Leishmaniasis Establishment in the Experimental Model of BALB/c Mice
    Article Snippet: .. Gene expression was determined by measuring the fluorescence signals in real time with the use of ViiA™7 Real Time PCR System (Applied Biosystems) and critical threshold values were generated using the ViiA™7 Software Version 1.2.1 (Applied Biosystems). .. HPRT was used as an internal control to calculate fold changes in target genes in spleen biopsy samples obtained from infected mice that have been previously vaccinated with CPA160−189 -loaded p8-PLGA nanoparticles and non-vaccinated infected mice, by use of 2−ΔΔCT method.

    Expressing:

    Article Title: Transcriptome Analysis Identifies Immune Markers Related to Visceral Leishmaniasis Establishment in the Experimental Model of BALB/c Mice
    Article Snippet: .. Gene expression was determined by measuring the fluorescence signals in real time with the use of ViiA™7 Real Time PCR System (Applied Biosystems) and critical threshold values were generated using the ViiA™7 Software Version 1.2.1 (Applied Biosystems). .. HPRT was used as an internal control to calculate fold changes in target genes in spleen biopsy samples obtained from infected mice that have been previously vaccinated with CPA160−189 -loaded p8-PLGA nanoparticles and non-vaccinated infected mice, by use of 2−ΔΔCT method.

    Article Title: B1a B cells require autophagy for metabolic homeostasis and self-renewal
    Article Snippet: .. qRT-PCR RNA was isolated using the RNeasy Micro kit (Qiagen) and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher). qRT-PCR was performed using Taqman Gene Expression Master Mix on a ViiA 7 instrument (Thermo Fisher). .. The PCR probes used are listed in Table S1.

    Polymerase Chain Reaction:

    Article Title: Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature
    Article Snippet: .. The ViiA7 (Life technologies) system was used to run the PCR with these conditions: 95°C for 10 min; 35 cycles of 95 °C, 60 °C, and 72 °C with 30 s each step. ..

    Software:

    Article Title: General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays
    Article Snippet: .. Example of an uninterpretable protein melting curve on the Viia7 instrument (Life Technologies) and analyzed with the Protein Thermal Shift (PTS) software (Life Technologies). ..

    Article Title: Transcriptome Analysis Identifies Immune Markers Related to Visceral Leishmaniasis Establishment in the Experimental Model of BALB/c Mice
    Article Snippet: .. Gene expression was determined by measuring the fluorescence signals in real time with the use of ViiA™7 Real Time PCR System (Applied Biosystems) and critical threshold values were generated using the ViiA™7 Software Version 1.2.1 (Applied Biosystems). .. HPRT was used as an internal control to calculate fold changes in target genes in spleen biopsy samples obtained from infected mice that have been previously vaccinated with CPA160−189 -loaded p8-PLGA nanoparticles and non-vaccinated infected mice, by use of 2−ΔΔCT method.

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