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Journal: Cell Reports Medicine

Article Title: Intracellular sclerostin promotes tumor progression and metastasis as a potential therapeutic target in triple-negative breast cancer

doi: 10.1016/j.xcrm.2026.102763

Figure Lengend Snippet:

Article Snippet: For the azide linked-VHL ligands with different linkers, which were generated by mixing the azide-linker-NHS esters (N3-PEG2-C2-NHS ester, leyan, Cat: 1175728) (N3-PEG4-CH2COONHS, ACHEMBLOCK, Cat: V156175) (Azido-PEG6-NHS ester, BROADPHARM, Cat: BP-21506) (Azido-PEG8-NHS ester, BROADPHARM, Cat: BP-215606) (Azido-PEG10-NHS ester, BROADPHARM, Cat: BP-23998) (1.0 eq), NH 2 -VHL ligand ((S, R, S)-AHPC, MedChemExpress, Cat: HY-125845) ((2S,4S)-1-((S)-2-Amino-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl) benzyl)pyrrolidine-2-carboxamide hydrochloride, bidepharm, Cat: BD01272772) (1.5 eq), and N,N-Diisopropylethylamine (MACKLIN, Cat: N741860) (1.5 eq) in DMSO for 4 h, respectively.

Techniques: Recombinant, Blocking Assay, Staining, Transfection, Silver Staining, Immunoprecipitation, Plasmid Preparation, Software