vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation"

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Figure Legend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation"

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Figure Legend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation"

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Figure Legend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation"

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Figure Legend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation"

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Figure Legend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Techniques Used: Expressing, Marker, MANN-WHITNEY

    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    na v 1 2  (Alomone Labs)


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    Alomone Labs na v 1 2
    Na V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti vglut1  (Alomone Labs)


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    Alomone Labs anti vglut1
    Table 1
    Anti Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord"

    Article Title: Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord

    Journal: Brain structure & function

    doi: 10.1007/s00429-015-1019-6

    Table 1
    Figure Legend Snippet: Table 1

    Techniques Used: Expressing

    anti vglut1  (Alomone Labs)


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    Alomone Labs anti vglut1
    Table 1
    Anti Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord"

    Article Title: Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord

    Journal: Brain structure & function

    doi: 10.1007/s00429-015-1019-6

    Table 1
    Figure Legend Snippet: Table 1

    Techniques Used: Expressing

    rabbit anti vglut1  (Alomone Labs)


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    Alomone Labs rabbit anti vglut1
    Rabbit Anti Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs vglut1
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    vglut1 - by Bioz Stars, 2023-01
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    88
    Alomone Labs na v 1 2
    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker <t>vGLUT1</t> (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).
    Na V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na v 1 2/product/Alomone Labs
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    na v 1 2 - by Bioz Stars, 2023-01
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    86
    Alomone Labs anti vglut1
    Table 1
    Anti Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vglut1/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vglut1 - by Bioz Stars, 2023-01
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    88
    Alomone Labs rabbit anti vglut1
    Table 1
    Rabbit Anti Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vglut1/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vglut1 - by Bioz Stars, 2023-01
    88/100 stars
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    Image Search Results


    Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Journal: The Journal of Neuroscience

    Article Title: Presynaptic L-Type Ca 2+ Channels Increase Glutamate Release Probability and Excitatory Strength in the Hippocampus during Chronic Neuroinflammation

    doi: 10.1523/JNEUROSCI.2981-19.2020

    Figure Lengend Snippet: Long-lasting LPS treatment increases the expression of L-type Ca2+ channels at glutamatergic synapses. A, Top, Representative images of neurites of hippocampal neurons exposed to either vehicle or LPS for 48 h and double-immunostained with Cav1.2 antibodies (green) and the excitatory presynaptic marker vGLUT1 (red). Merge panels represent synaptic puncta in which Cav1.2 and vGLUT1 colocalize (yellow; arrows). Bottom, Representative immunoreactivity intensity profiles of the colocalization experiments showing the increased occurrence of overlap between Cav1.2- and vGLUT1-positive puncta in the samples chronically treated with LPS. B, Quantification of the density of vGLUT1-positive puncta counted on 30 μm dendrite tracts starting from the neuronal body. Data are mean ± SEM from three independent experiments, each conducted in duplicate (veh = 30; LPS = 29). C, Quantification of the absolute Cav1.2/vGLUT1 colocalization area (top) and of the Cav1.2/vGLUT1 colocalization expressed in percent of the total Cav1.2-immunopositive area (bottom) in vehicle- and LPS-treated neurons (veh = 66; LPS = 97). Data are mean ± SEM. **p < 0.01 (unpaired Student's t test/Mann–Whitney U test).

    Article Snippet: The presynaptic localization of the L-type Ca 2+ channel was analyzed in treated neurons by evaluating the immunoreactivity of L-type Ca 2+ channel (1:200, Alomone Labs) ( Hermosilla et al., 2017 ) in vGLUT1-positive excitatory boutons.

    Techniques: Expressing, Marker, MANN-WHITNEY

    Table 1

    Journal: Brain structure & function

    Article Title: Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord

    doi: 10.1007/s00429-015-1019-6

    Figure Lengend Snippet: Table 1

    Article Snippet: Antibodies included rabbit anti-ATF3 (1:4000; sc-188, Santa Cruz, Dallas, TX, USA), anti-CGRP (1:12,000; C8198, Sigma, Saint Louis, MO, USA), anti-TRPV1 (1:4000; ACC-030, Alomone Labs, Jerusalem, Israel), anti-TH (1:4000; AB152, Millipore, Temecula, CA, USA), anti-VGLUT1 (1:4000; Brumovsky et al. 2007 , 2011a , b; Kawamura et al. 2006 ) or guinea pig anti-VGLUT2 (1:4000; Brumovsky et al. 2007 , 2011a , b; Miyazaki et al. 2003 ).

    Techniques: Expressing