african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg  (ATCC)


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    ATCC african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg
    African Green Monkey Kidney Vero Normal Fibroblast Cell Line Atcc Ccl 81 Vhg, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monkey kidney epithelial vero stat1 ko cell line  (ATCC)


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    ATCC monkey kidney epithelial vero stat1 ko cell line
    Monkey Kidney Epithelial Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg  (ATCC)


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    ATCC african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg
    African Green Monkey Kidney Vero Normal Fibroblast Cell Line Atcc Ccl 81 Vhg, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl 81vhg cell lines  (ATCC)


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    ATCC ccl 81vhg cell lines
    Ccl 81vhg Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 ko cell line  (ATCC)


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    ATCC vero stat1 ko cell line
    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in <t>Vero.STAT1</t> KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "(E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression"

    Article Title: (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression

    Journal: Viruses

    doi: 10.3390/v13040712

    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Techniques Used: Expressing, Activity Assay, Infection, Transfection, Luciferase, Activation Assay, Quantitative RT-PCR, Western Blot

    african green monkey kidney cell line vero cells  (ATCC)


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    ATCC african green monkey kidney cell line vero cells
    African Green Monkey Kidney Cell Line Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney vero normal fibroblast cell line atcc ccl 81 vhg
    African Green Monkey Kidney Vero Normal Fibroblast Cell Line Atcc Ccl 81 Vhg, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC monkey kidney epithelial vero stat1 ko cell line
    Monkey Kidney Epithelial Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ccl 81vhg cell lines
    Ccl 81vhg Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC vero stat1 ko cell line
    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in <t>Vero.STAT1</t> KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    Vero Stat1 Ko Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    ATCC african green monkey kidney cell line vero cells
    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in <t>Vero.STAT1</t> KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.
    African Green Monkey Kidney Cell Line Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/african green monkey kidney cell line vero cells/product/ATCC
    Average 93 stars, based on 1 article reviews
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    african green monkey kidney cell line vero cells - by Bioz Stars, 2024-05
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    (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Journal: Viruses

    Article Title: (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression

    doi: 10.3390/v13040712

    Figure Lengend Snippet: (E)-Guggulsterone induces IFN-mediated antiviral gene expression. ( a , b ) (E)-Guggulsterone induced the ISRE promoter activity in parental Huh-7 and DENV-infected Huh-7 cells. Huh-7 cells were transfected with pISR-Luc and then treated with the indicated concentrations of guggulsterone (0–20 μM) for 3 days. The relative induction of ISRE promoter activity was determined using a luciferase assay. The relative induction of promoter activity was determined by luciferase assay and presented as fold activation compared to the DENV-infected cells treated with 0.1% DMSO, designed as “0”. (E)-Guggulsterone increased the mRNA levels of ( c ) OAS1, ( d ) OAS2, and ( e ) OAS3 in DENV-infected Huh-7 cells. DENV-infected Huh-7 cells were treated with the indicated (E)-guggulsterone concentrations (0–20 μM) for 3 days. The mRNA level of OAS1-3 was analyzed using RT-qPCR. The relative percent induction was compared to the DENV-infected cells treated with 0.1% DMSO, defined as 100%. ( f ) (E)-guggulsterone exhibited slightly antiviral activity in Vero.STAT1 KO cells. DENV-infected Vero.STAT1 KO cells were treated with the indicated concentrations of (E)-guggulsterone for 3 days, and the DENV protein levels were analyzed by Western blotting. Here, “0” was regarded as the treatment with 0.1% DMSO. Error bars indicate the mean ± SD of five independent experiments ( n = 5). * p < 0.05, ** p < 0.01.

    Article Snippet: Vero.STAT1 KO cell line, a STAT1 gene knockout Vero cells, was purchased from American Type Culture Collection (ATCC, Manassas, WV, USA; ATCC number CCL-81-VHG TM ).

    Techniques: Expressing, Activity Assay, Infection, Transfection, Luciferase, Activation Assay, Quantitative RT-PCR, Western Blot