vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.741502

    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Infection, Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    Pearson’s product moment correlation coefficient of IFN pathway-related genes.
    Figure Legend Snippet: Pearson’s product moment correlation coefficient of IFN pathway-related genes.

    Techniques Used:

    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants"

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    Journal: Journal of Virology

    doi: 10.1128/JVI.01437-21

    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Figure Legend Snippet: Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

    Techniques Used: MTT Assay, Concentration Assay

    SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.
    Figure Legend Snippet: SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

    Techniques Used: Infection, Knock-Out, Inhibition

    Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.
    Figure Legend Snippet: Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.

    Techniques Used: Knock-Out, Infection

    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: bioRxiv

    doi: 10.1101/2021.07.07.449660

    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Panel I: Measurement of 50% cytotoxic concentration (CC 50 ) of five drug-like compounds. (A to E) Viability of <t>Vero-STAT1</t> knockout cells in the presence of an indicated concentration of the compounds at 37°C for 72 hrs as measured by MTT assay. The CC 50 values were computed using four-parameter variable slope sigmoidal dose-response models with Graph Pad Prism 8.0 software. Panel II: Screening entry inhibition potential of drug-like compounds in HEK293 cells expressing human ACE2. (F to J) HEK293-ACE2 cells were pretreated with the indicated concentration of compounds and then inoculated with pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2. At 48 hrs post-inoculation, pseudotype entry was analyzed after normalization against untreated cells by determining luciferase activity in cell lysates. (K) The percentage inhibition of entry of pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2 was measured for MU-UNMC-2 at indicated concentration of the compounds (cells that received DMSO were considered untreated controls). The IC 50 value was computed using four parameter variable slope sigmoidal dose-response models using Graph Pad Prism 8.0 software.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Discovery and in-vitro evaluation of potent SARS-CoV-2 entry inhibitors"

    Article Title: Discovery and in-vitro evaluation of potent SARS-CoV-2 entry inhibitors

    Journal: bioRxiv

    doi: 10.1101/2021.04.02.438204

    Panel I: Measurement of 50% cytotoxic concentration (CC 50 ) of five drug-like compounds. (A to E) Viability of Vero-STAT1 knockout cells in the presence of an indicated concentration of the compounds at 37°C for 72 hrs as measured by MTT assay. The CC 50 values were computed using four-parameter variable slope sigmoidal dose-response models with Graph Pad Prism 8.0 software. Panel II: Screening entry inhibition potential of drug-like compounds in HEK293 cells expressing human ACE2. (F to J) HEK293-ACE2 cells were pretreated with the indicated concentration of compounds and then inoculated with pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2. At 48 hrs post-inoculation, pseudotype entry was analyzed after normalization against untreated cells by determining luciferase activity in cell lysates. (K) The percentage inhibition of entry of pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2 was measured for MU-UNMC-2 at indicated concentration of the compounds (cells that received DMSO were considered untreated controls). The IC 50 value was computed using four parameter variable slope sigmoidal dose-response models using Graph Pad Prism 8.0 software.
    Figure Legend Snippet: Panel I: Measurement of 50% cytotoxic concentration (CC 50 ) of five drug-like compounds. (A to E) Viability of Vero-STAT1 knockout cells in the presence of an indicated concentration of the compounds at 37°C for 72 hrs as measured by MTT assay. The CC 50 values were computed using four-parameter variable slope sigmoidal dose-response models with Graph Pad Prism 8.0 software. Panel II: Screening entry inhibition potential of drug-like compounds in HEK293 cells expressing human ACE2. (F to J) HEK293-ACE2 cells were pretreated with the indicated concentration of compounds and then inoculated with pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2. At 48 hrs post-inoculation, pseudotype entry was analyzed after normalization against untreated cells by determining luciferase activity in cell lysates. (K) The percentage inhibition of entry of pseudotyped lentiviral particles expressing spike glycoprotein of SARS-CoV-2 was measured for MU-UNMC-2 at indicated concentration of the compounds (cells that received DMSO were considered untreated controls). The IC 50 value was computed using four parameter variable slope sigmoidal dose-response models using Graph Pad Prism 8.0 software.

    Techniques Used: Concentration Assay, Knock-Out, MTT Assay, Software, Inhibition, Expressing, Luciferase, Activity Assay

    (A & B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hrs post-infection in UNCN1T cells with indicated drug concentrations. (C & D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hrs post-infection in Vero-STAT1 knockout cells with indicated drug concentrations.
    Figure Legend Snippet: (A & B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hrs post-infection in UNCN1T cells with indicated drug concentrations. (C & D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hrs post-infection in Vero-STAT1 knockout cells with indicated drug concentrations.

    Techniques Used: Inhibition, Infection, Knock-Out

    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    (A & B) Real-time quantitative PCR analysis of SARS-CoV-2 genome equivalent per ml of culture supernatant after 24 hrs post-infection in SF2523 (in blue) and remdesivir (in green) treated UNCN1T cells and <t>Vero-STAT1</t> knockout cells with indicated drug concentrations, respectively. (C & D) SF2523 (in blue) and remdesivir (in green) dose response curve by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection in UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations, respectively.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Blockade of SARS-CoV-2 infection in vitro by highly potent PI3K-α/mTOR/BRD4 inhibitor"

    Article Title: Blockade of SARS-CoV-2 infection in vitro by highly potent PI3K-α/mTOR/BRD4 inhibitor

    Journal: bioRxiv

    doi: 10.1101/2021.03.02.433604

    (A & B) Real-time quantitative PCR analysis of SARS-CoV-2 genome equivalent per ml of culture supernatant after 24 hrs post-infection in SF2523 (in blue) and remdesivir (in green) treated UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations, respectively. (C & D) SF2523 (in blue) and remdesivir (in green) dose response curve by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection in UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations, respectively.
    Figure Legend Snippet: (A & B) Real-time quantitative PCR analysis of SARS-CoV-2 genome equivalent per ml of culture supernatant after 24 hrs post-infection in SF2523 (in blue) and remdesivir (in green) treated UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations, respectively. (C & D) SF2523 (in blue) and remdesivir (in green) dose response curve by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection in UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations, respectively.

    Techniques Used: Real-time Polymerase Chain Reaction, Infection, Knock-Out, Inhibition

    ( A & B ) Real-time quantitative PCR analysis of SARS-CoV-2 genome equivalent per ml of culture supernatant after 48 hrs post-infection in SF2523 (in blue) and remdesivir (in green) treated UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations respectively. ( C & D ) SF2523 (in blue) and remdesivir (in green) dose response curve by percentage inhibition of SARS-CoV-2 replication 48 hrs post infection in UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations respectively. In UNCN1T cells, SF2523 has an IC 50 of 1.58 μM and remdesivir has an IC 50 of 2.75 μM; In Vero-STAT1 knockout cells, SF2523 has an IC 50 of 3.22 μM and remdesivir has an IC 50 of 0.76 μM.
    Figure Legend Snippet: ( A & B ) Real-time quantitative PCR analysis of SARS-CoV-2 genome equivalent per ml of culture supernatant after 48 hrs post-infection in SF2523 (in blue) and remdesivir (in green) treated UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations respectively. ( C & D ) SF2523 (in blue) and remdesivir (in green) dose response curve by percentage inhibition of SARS-CoV-2 replication 48 hrs post infection in UNCN1T cells and Vero-STAT1 knockout cells with indicated drug concentrations respectively. In UNCN1T cells, SF2523 has an IC 50 of 1.58 μM and remdesivir has an IC 50 of 2.75 μM; In Vero-STAT1 knockout cells, SF2523 has an IC 50 of 3.22 μM and remdesivir has an IC 50 of 0.76 μM.

    Techniques Used: Real-time Polymerase Chain Reaction, Infection, Knock-Out, Inhibition

    ( A ) Dose response curve of remdesivir (green: IC 50 = 1.06 μM), (blue: IC 50 = 1.52 μM), and remdesivir with a fixed dose combination of SF2523 (0.1 μM) (red: IC 50 = 0.625) by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection in UNCN1T cells; ( B ) Dose response curve of remdesivir (green: IC 50 = 1.03μM), (blue: IC 50 = 1.02 μM), and SF2523 with a fixed dose combination of remdesivir (0.05μM) (red: IC 50 = 1.21) by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection (IC 50 = 1.02 μM) in Vero-STAT1 knockout cells.
    Figure Legend Snippet: ( A ) Dose response curve of remdesivir (green: IC 50 = 1.06 μM), (blue: IC 50 = 1.52 μM), and remdesivir with a fixed dose combination of SF2523 (0.1 μM) (red: IC 50 = 0.625) by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection in UNCN1T cells; ( B ) Dose response curve of remdesivir (green: IC 50 = 1.03μM), (blue: IC 50 = 1.02 μM), and SF2523 with a fixed dose combination of remdesivir (0.05μM) (red: IC 50 = 1.21) by percentage inhibition of SARS-CoV-2 replication 24 hrs post infection (IC 50 = 1.02 μM) in Vero-STAT1 knockout cells.

    Techniques Used: Inhibition, Infection, Knock-Out

    (A) The Median-Effect plot (Chou plot) of remdesivir (the red line with square data points, SF2523 (blue line with circular data points) and fixed dose combination of remdesivir and SF2523 (green line with triangular data points). The median-Effect equation, that describes the dose-effect relationship is given by (Fa/Fu) = (D/Dm) m or log(Fa/Fu) = m log(D) – m log(Dm). The slope (m) of the lines signifies the shape (m = 1, >1, and <1 signify hyperbolic, sigmoidal, and flat sigmoidal dose-effect curves respectively). Fa is defined as percentage inhibition of viral growth at dose D and Fu is the fraction that remain unaffected (i.e., Fu = 1 - Fa). The antilog of x-intercept, where Fa/Fu = 1 or log(Fa/Fu) = 0, gives the Dm value. [log(Dm)] signifies the potency of the drugs (where Dm stands for median effective dose or IC 50 concentration: The concentration required to inhibit 50% growth of the virus). ( B ) Combination index plot or Chou-Talaly plot or Fa-CI plot: A plot of CI (combination index) on the Y-axis vs Fa on X-axis for fixed dose combination of remdesivir and SF2523 in SARS-CoV-2 infected Vero-STAT1 knockout cells (blue line with circular data points); Low CI values with increase in Fa values indicates superior compatibility and high synergism between drugs combination (based on Chou and Talalay Combination Index Theorem, CI < 1, = 1, and >1 indicate synergism, additive effect, and antagonism, respectively). ( C ) Dose-response percent inhibition matrix of single and combined treatment of remdesivir and SF2523 in SARS-CoV-2 infected Vero-STAT1 knockout cells 24 hrs post infection. ( D ) 3-D interaction landscape between remdesivir and SF2523 calculated based on Loewe additive model using SynergyFinder v.2 in SARS-CoV-2 infected Vero-STAT1 knockout cells 24 hrs post infection (Loewe synergy score -0.20; with most synergistic area score of 7.67). Synergy maps highlight synergistic and antagonistic dose regions in red and green colors, respectively. Although there is no defined threshold, for drug combinations with a synergy scores above -10 is considered likely antagonistic, score between -10 to 10 is considered likely additive and score above 10 is considered synergistic.
    Figure Legend Snippet: (A) The Median-Effect plot (Chou plot) of remdesivir (the red line with square data points, SF2523 (blue line with circular data points) and fixed dose combination of remdesivir and SF2523 (green line with triangular data points). The median-Effect equation, that describes the dose-effect relationship is given by (Fa/Fu) = (D/Dm) m or log(Fa/Fu) = m log(D) – m log(Dm). The slope (m) of the lines signifies the shape (m = 1, >1, and <1 signify hyperbolic, sigmoidal, and flat sigmoidal dose-effect curves respectively). Fa is defined as percentage inhibition of viral growth at dose D and Fu is the fraction that remain unaffected (i.e., Fu = 1 - Fa). The antilog of x-intercept, where Fa/Fu = 1 or log(Fa/Fu) = 0, gives the Dm value. [log(Dm)] signifies the potency of the drugs (where Dm stands for median effective dose or IC 50 concentration: The concentration required to inhibit 50% growth of the virus). ( B ) Combination index plot or Chou-Talaly plot or Fa-CI plot: A plot of CI (combination index) on the Y-axis vs Fa on X-axis for fixed dose combination of remdesivir and SF2523 in SARS-CoV-2 infected Vero-STAT1 knockout cells (blue line with circular data points); Low CI values with increase in Fa values indicates superior compatibility and high synergism between drugs combination (based on Chou and Talalay Combination Index Theorem, CI < 1, = 1, and >1 indicate synergism, additive effect, and antagonism, respectively). ( C ) Dose-response percent inhibition matrix of single and combined treatment of remdesivir and SF2523 in SARS-CoV-2 infected Vero-STAT1 knockout cells 24 hrs post infection. ( D ) 3-D interaction landscape between remdesivir and SF2523 calculated based on Loewe additive model using SynergyFinder v.2 in SARS-CoV-2 infected Vero-STAT1 knockout cells 24 hrs post infection (Loewe synergy score -0.20; with most synergistic area score of 7.67). Synergy maps highlight synergistic and antagonistic dose regions in red and green colors, respectively. Although there is no defined threshold, for drug combinations with a synergy scores above -10 is considered likely antagonistic, score between -10 to 10 is considered likely additive and score above 10 is considered synergistic.

    Techniques Used: Inhibition, Concentration Assay, Infection, Knock-Out

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