vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    stat1 gene knockout vero cells  (ATCC)


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    ATCC stat1 gene knockout vero cells
    Stat1 Gene Knockout Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
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    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
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    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.741502

    SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: SARS-CoV-2 restrictive infection of human MDMs. (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in cell lysates and culture supernatants by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. ( C .i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs [( C. ii) day 5, and ( C. iii) day 14 after viral exposure]. Red arrows, circles, and boxes demonstrate clusters of viral particles within the virus-challenged macrophages. All experiments were done at least twice with representative images depicted here. (A, B) Data are represented as mean ± SEM (n=3-6 donors). Statistical significance between groups was determined using one-way ANOVA, and p < 0.05 was considered significant (*significantly different from day 0 w/o captopril, a: significantly different from day 1 w/o captopril, b: significantly different from day 5 w/o captopril, a w : significantly different from day 1 with captopril, b w : significantly different from day 3 with captopril, c w : significantly different from day 5 with captopril). w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Infection, Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

    Pearson’s product moment correlation coefficient of IFN pathway-related genes.
    Figure Legend Snippet: Pearson’s product moment correlation coefficient of IFN pathway-related genes.

    Techniques Used:

    vero stat1 knockout cells  (ATCC)


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    ATCC vero stat1 knockout cells
    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in <t>Vero-STAT1</t> KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Vero Stat1 Knockout Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants"

    Article Title: Discovery and Evaluation of Entry Inhibitors for SARS-CoV-2 and Its Emerging Variants

    Journal: Journal of Virology

    doi: 10.1128/JVI.01437-21

    Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.
    Figure Legend Snippet: Measurement of cytotoxicity of five drug-like compounds using MTT assay. Measurement of cytotoxicity of five drug-like compounds. (A–E) Viability of HEK293T-hACE2 cells in the presence of an indicated concentration of the compounds. (F, G) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Vero-STAT1 KO cells in the presence of an indicated concentration of the compounds. (H, I) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in UNCN1T cells in the presence of an indicated concentration of the compounds. (J, K) Measurement of cytotoxicity of MU-UNMC-1 and MU-UNMC-2 in Calu-3 cells in the presence of an indicated concentration of the compounds.

    Techniques Used: MTT Assay, Concentration Assay

    SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.
    Figure Legend Snippet: SARS-CoV-2 dose-response curve in MU-UNMC-1 and MU-UNMC-2 treated and SARS-CoV-2 infected UNCN1T and Vero-STAT1 knockout cells. (A, B) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in UNCN1T cells with indicated drug concentrations. (C, D) MU-UNMC-1 (in blue) and MU-UNMC-2 (in green) dose-response curve by percentage inhibition of SARS-CoV-2 replication 24 and 48 hpi in Vero-STAT1 knockout cells with indicated compound concentrations.

    Techniques Used: Infection, Knock-Out, Inhibition

    Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.
    Figure Legend Snippet: Impact of time addition of the compounds on replication of SARS-CoV-2 in Vero-STAT1 knockout cells. (A) An experimental outline describing the time of adding MU-UNMC-1 and MU-UNMC-2 to the cells, SARS-CoV-2 infection, and measurement of viral replication kinetics at the termination of the experiment. (B) Percentage of SARS-CoV-2 replication in the presence of vehicle control (DMSO), MU-UNMC-1 (5 μM) and MU-UNMC-2 (5 μM) at -2 hpi, +0 hpi and +4 hpi in Vero-STAT1 knockout cells, respectively.

    Techniques Used: Knock-Out, Infection

    vero stat1 knockout ko cells  (ATCC)


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    ATCC vero stat1 knockout ko cells
    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. <t>Vero.STAT1</t> KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Vero Stat1 Knockout Ko Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions"

    Article Title: Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions

    Journal: bioRxiv

    doi: 10.1101/2021.07.07.449660

    (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).
    Figure Legend Snippet: (A) Expression of SARS-CoV-2 cell entry receptor ACE2 and phenotypic surface markers CD14 and CD16, during differentiation of monocytes into macrophages, was analyzed by flow cytometry in absence or presence of captopril. (B) SARS-CoV-2 presence in MDMs. SARS-CoV-2 (MOI=0.01) was used to infect MDMs (with or without captopril). The number of virus genome equivalents per ml was measured in the culture supernatant by RT-qPCR. Vero.STAT1 KO cells served as a positive control. (C) Transmission electron micrographs of SARS-CoV-2-challenged MDMs. (C.i) A viral particle from a pool of SARS-CoV-2 used for the virus challenges. SARS-CoV-2-challenged MDMs (C.ii: day 5, and C.iii: day 14 after viral exposure). Red arrows, circles, and boxes demonstrate clusters of viral particles within the macrophage cytoplasm. All experiments were done at least twice with representative images depicted here. ( A and B ) Data are represented as mean ± SEM (n=3-6). Statistical significance between groups was determined using unpaired Student’s t-test or one-way ANOVA, and p < 0.05 was considered significant (*** p < 0.001). Abbreviations: w/o: without. Scale bars: 50 nm (i and left panel of iii), 100 nm (left panel of ii), and 400 nm (right panels of ii and iii).

    Techniques Used: Expressing, Flow Cytometry, Quantitative RT-PCR, Positive Control, Transmission Assay

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    ATCC vero stat1 knockout cells
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