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BEI Resources vero cells
Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with <t>ZIKV</t> (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) <t>Vero</t> cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.
Vero Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero cells - by Bioz Stars, 2021-06
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1) Product Images from "Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions"

Article Title: Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions

Journal: Journal of Virology

doi: 10.1128/JVI.00530-19

Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.
Figure Legend Snippet: Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.

Techniques Used: Infection, Plaque Assay, Quantitative RT-PCR

2) Product Images from "Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions"

Article Title: Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions

Journal: Journal of Virology

doi: 10.1128/JVI.00530-19

Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P  ≤ 0.05; **, P  ≤ 0.01; ***, P  ≤ 0.001 (using Student’s t test [ n  ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.
Figure Legend Snippet: Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P  ≤ 0.05; **, P  ≤ 0.01; ***, P  ≤ 0.001 (using Student’s t test [ n  ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.

Techniques Used: Infection, Plaque Assay, Quantitative RT-PCR

3) Product Images from "Establishment of a Replicon System for Bourbon Virus and Identification of Small Molecules that Efficiently Inhibit Virus Replication"

Article Title: Establishment of a Replicon System for Bourbon Virus and Identification of Small Molecules that Efficiently Inhibit Virus Replication

Journal: bioRxiv

doi: 10.1101/2020.04.24.058693

Determination of the CC 50 and EC 50 of compound #9 in cell cultures. (A B) EC 50 of compound #9. HEK293T cells (A) or Vero cells (B) were infected with BRBV at an MOI of 10, followed by application of compound #9 at various concentrations as indicated. At 3 days post treatment, RT-qPCR was performed to quantify the viral genome copy (vgc) numbers in the cell culture media. Values are the means with standard deviation and are normalized to a mock group, and were obtained from three experiments, each performed in duplicate. The EC 50 was calculated using GraphPad Prism software. (C D) CC 50 of compound #9 . A cytotoxicity assay was used to measure the viability of HEK293T cells (C) or Vero cells (D) affected by compound #9 at various concentrations as indicated. The percentage of viable cells were determined using a CytoTox-Glo cytotoxicity assay kit (Promega). The results are shown as relative values to the mock control cells. Values are the means with standard deviation obtained from three experiments performed in duplicate. The CC 50 was calculated using GraphPad Prism software.
Figure Legend Snippet: Determination of the CC 50 and EC 50 of compound #9 in cell cultures. (A B) EC 50 of compound #9. HEK293T cells (A) or Vero cells (B) were infected with BRBV at an MOI of 10, followed by application of compound #9 at various concentrations as indicated. At 3 days post treatment, RT-qPCR was performed to quantify the viral genome copy (vgc) numbers in the cell culture media. Values are the means with standard deviation and are normalized to a mock group, and were obtained from three experiments, each performed in duplicate. The EC 50 was calculated using GraphPad Prism software. (C D) CC 50 of compound #9 . A cytotoxicity assay was used to measure the viability of HEK293T cells (C) or Vero cells (D) affected by compound #9 at various concentrations as indicated. The percentage of viable cells were determined using a CytoTox-Glo cytotoxicity assay kit (Promega). The results are shown as relative values to the mock control cells. Values are the means with standard deviation obtained from three experiments performed in duplicate. The CC 50 was calculated using GraphPad Prism software.

Techniques Used: Infection, Quantitative RT-PCR, Cell Culture, Standard Deviation, Software, Cytotoxicity Assay

Comparisons of BRBV-KS infection between Vero and HEK293T cells (A) BRBV growth kinetics. Cells were infected with BRBV-KS at an MOI=10 pfu/cell. Cell culture media (supernatants) were collected daily for 6 days. Viral RNA was extracted from the samples of supernatants and then titrated by a RT-qPCR assay. The data presented the means with standard deviations, which are obtained from three independent experiments, with each experiment analyzing samples in duplicate. (B) Western blotting. The M protein in BRBV-infected Vero and HEK293T cells was detected at ~30 kDa as indicated, but not in mock-infected cells. β-action was probed as a loading control.
Figure Legend Snippet: Comparisons of BRBV-KS infection between Vero and HEK293T cells (A) BRBV growth kinetics. Cells were infected with BRBV-KS at an MOI=10 pfu/cell. Cell culture media (supernatants) were collected daily for 6 days. Viral RNA was extracted from the samples of supernatants and then titrated by a RT-qPCR assay. The data presented the means with standard deviations, which are obtained from three independent experiments, with each experiment analyzing samples in duplicate. (B) Western blotting. The M protein in BRBV-infected Vero and HEK293T cells was detected at ~30 kDa as indicated, but not in mock-infected cells. β-action was probed as a loading control.

Techniques Used: Infection, Cell Culture, Quantitative RT-PCR, Western Blot

Titration of BRBV stock. (A) Plaque assay. Vero cell monolayers on a 6-well plate were infected with 200 μl of serial 10-fold diluted BRBV-KS as indicated. Plaques were visualized by staining with crystal violet after 5 days of infection on Vero cells. Arrows indicate plaques. (B) Comparison between plaque assay and RT-qPCR. The same virus stock was diluted by serial 10-fold dilution. RT-qPCR was used to quantify the viral genome copy (vgc) number. The linear regression has a R 2 of 0.9875, indicating 1 plaque forming unit (pfu) equals ~100 vgc. The data presented are averages and standard deviations from three independent experiments, with each experiment analyzing samples in duplicate.
Figure Legend Snippet: Titration of BRBV stock. (A) Plaque assay. Vero cell monolayers on a 6-well plate were infected with 200 μl of serial 10-fold diluted BRBV-KS as indicated. Plaques were visualized by staining with crystal violet after 5 days of infection on Vero cells. Arrows indicate plaques. (B) Comparison between plaque assay and RT-qPCR. The same virus stock was diluted by serial 10-fold dilution. RT-qPCR was used to quantify the viral genome copy (vgc) number. The linear regression has a R 2 of 0.9875, indicating 1 plaque forming unit (pfu) equals ~100 vgc. The data presented are averages and standard deviations from three independent experiments, with each experiment analyzing samples in duplicate.

Techniques Used: Titration, Plaque Assay, Infection, Staining, Quantitative RT-PCR

4) Product Images from "In vivo rescue of recombinant Zika virus from an infectious cDNA clone and its implications in vaccine development"

Article Title: In vivo rescue of recombinant Zika virus from an infectious cDNA clone and its implications in vaccine development

Journal: Scientific Reports

doi: 10.1038/s41598-020-57545-2

In vivo rescue of rZIKV from Vero cells transfected with the BAC cDNA clone. (A) Schematic representation of rZIKV in vivo rescue approach using transfected Vero cells. ( B–D) Four-to-five-week-old IFNAR−/− A129 male (N = 3) and female (N = 3) mice were inoculated SC in the footpad (top), IM in the quadriceps muscle (middle) or IP in the peritoneal cavity (bottom) with 1 × 10 6 Vero cells/mouse transfected with pBAC-ZIKV. Body weight ( B ) and survival ( C ) were evaluated during 18 dpi. Mice that lost more than 20% of their initial body weight or presented hind limb paralysis were humanely euthanized. Error bars represent standard deviations (SD) of the mean for each group of mice. Mice were bled at 3 and 6 dpi and viral titers in sera were determined by immunofocus assay (FFU/ml) ( D ). Symbols represent data from individual mice and bars the geometric means of viral titers. *Virus not detected in one mouse; , virus not detected in two mice; ND, not detected. Dotted black lines indicate the limit of detection, LoD (200 FFU/ml). No statistically significant differences between the SC, IM and IP routes of inoculation of transfected cells were observed.
Figure Legend Snippet: In vivo rescue of rZIKV from Vero cells transfected with the BAC cDNA clone. (A) Schematic representation of rZIKV in vivo rescue approach using transfected Vero cells. ( B–D) Four-to-five-week-old IFNAR−/− A129 male (N = 3) and female (N = 3) mice were inoculated SC in the footpad (top), IM in the quadriceps muscle (middle) or IP in the peritoneal cavity (bottom) with 1 × 10 6 Vero cells/mouse transfected with pBAC-ZIKV. Body weight ( B ) and survival ( C ) were evaluated during 18 dpi. Mice that lost more than 20% of their initial body weight or presented hind limb paralysis were humanely euthanized. Error bars represent standard deviations (SD) of the mean for each group of mice. Mice were bled at 3 and 6 dpi and viral titers in sera were determined by immunofocus assay (FFU/ml) ( D ). Symbols represent data from individual mice and bars the geometric means of viral titers. *Virus not detected in one mouse; , virus not detected in two mice; ND, not detected. Dotted black lines indicate the limit of detection, LoD (200 FFU/ml). No statistically significant differences between the SC, IM and IP routes of inoculation of transfected cells were observed.

Techniques Used: In Vivo, Transfection, BAC Assay, Mouse Assay

5) Product Images from "In vivo rescue of recombinant Zika virus from an infectious cDNA clone and its implications in vaccine development"

Article Title: In vivo rescue of recombinant Zika virus from an infectious cDNA clone and its implications in vaccine development

Journal: Scientific Reports

doi: 10.1038/s41598-020-57545-2

In vivo rescue of rZIKV from Vero cells transfected with the BAC cDNA clone. (A) Schematic representation of rZIKV in vivo rescue approach using transfected Vero cells. ( B–D) Four-to-five-week-old IFNAR−/− A129 male (N = 3) and female (N = 3) mice were inoculated SC in the footpad (top), IM in the quadriceps muscle (middle) or IP in the peritoneal cavity (bottom) with 1 × 10 6 Vero cells/mouse transfected with pBAC-ZIKV. Body weight ( B ) and survival ( C ) were evaluated during 18 dpi. Mice that lost more than 20% of their initial body weight or presented hind limb paralysis were humanely euthanized. Error bars represent standard deviations (SD) of the mean for each group of mice. Mice were bled at 3 and 6 dpi and viral titers in sera were determined by immunofocus assay (FFU/ml) ( D ). Symbols represent data from individual mice and bars the geometric means of viral titers. *Virus not detected in one mouse; , virus not detected in two mice; ND, not detected. Dotted black lines indicate the limit of detection, LoD (200 FFU/ml). No statistically significant differences between the SC, IM and IP routes of inoculation of transfected cells were observed.
Figure Legend Snippet: In vivo rescue of rZIKV from Vero cells transfected with the BAC cDNA clone. (A) Schematic representation of rZIKV in vivo rescue approach using transfected Vero cells. ( B–D) Four-to-five-week-old IFNAR−/− A129 male (N = 3) and female (N = 3) mice were inoculated SC in the footpad (top), IM in the quadriceps muscle (middle) or IP in the peritoneal cavity (bottom) with 1 × 10 6 Vero cells/mouse transfected with pBAC-ZIKV. Body weight ( B ) and survival ( C ) were evaluated during 18 dpi. Mice that lost more than 20% of their initial body weight or presented hind limb paralysis were humanely euthanized. Error bars represent standard deviations (SD) of the mean for each group of mice. Mice were bled at 3 and 6 dpi and viral titers in sera were determined by immunofocus assay (FFU/ml) ( D ). Symbols represent data from individual mice and bars the geometric means of viral titers. *Virus not detected in one mouse; , virus not detected in two mice; ND, not detected. Dotted black lines indicate the limit of detection, LoD (200 FFU/ml). No statistically significant differences between the SC, IM and IP routes of inoculation of transfected cells were observed.

Techniques Used: In Vivo, Transfection, BAC Assay, Mouse Assay

Related Articles

other:

Article Title: Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice
Article Snippet: This stock was passaged three times in Vero E6 cells (obtained through BEI Resources, NIAID, NIH: VERO C1008 (E6), Kidney (African green monkey), Working Cell Bank, NR-596) in MEM-α, L-alanyl-L-glutamine without nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified HI-FBS.

Derivative Assay:

Article Title: Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions
Article Snippet: MP-12 , LACV, and KEYV were derived from the first passage of virus in Huh7 cells. .. ZIKV (MR766) was derived from the first passage of virus in Vero cells. .. CVB3 (Nancy strain) was derived from the first passage of virus in HeLa cells.

RNA Extraction:

Article Title: Rescue of SARS-CoV-2 from a single bacterial artificial chromosome
Article Snippet: At the indicated times after infection, viral titers in tissue culture supernatants were determined by plaque assay and immunostaining using the N monoclonal antibody 1C7, as previously described . .. RNA extraction and RT-PCR Total RNA from SARS-CoV-2 USA-WA1/2020 or rSARS-CoV-2 infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. RT-PCR amplification of the viral genome spanning nucleotides 26,488-27,784, was performed using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR System (Sigma Aldrich).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Rescue of SARS-CoV-2 from a single bacterial artificial chromosome
Article Snippet: At the indicated times after infection, viral titers in tissue culture supernatants were determined by plaque assay and immunostaining using the N monoclonal antibody 1C7, as previously described . .. RNA extraction and RT-PCR Total RNA from SARS-CoV-2 USA-WA1/2020 or rSARS-CoV-2 infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. RT-PCR amplification of the viral genome spanning nucleotides 26,488-27,784, was performed using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR System (Sigma Aldrich).

Infection:

Article Title: Rescue of SARS-CoV-2 from a single bacterial artificial chromosome
Article Snippet: At the indicated times after infection, viral titers in tissue culture supernatants were determined by plaque assay and immunostaining using the N monoclonal antibody 1C7, as previously described . .. RNA extraction and RT-PCR Total RNA from SARS-CoV-2 USA-WA1/2020 or rSARS-CoV-2 infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. RT-PCR amplification of the viral genome spanning nucleotides 26,488-27,784, was performed using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR System (Sigma Aldrich).

Article Title: Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12
Article Snippet: .. Virus infection SARS-CoV-2 (strain USA-WA1/2020 isolate; BEI Resources, catalog no. NR-52281) was amplified in Vero E6 cells. .. Amplified stock virus was stored at −80°C until use.

Amplification:

Article Title: Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12
Article Snippet: .. Virus infection SARS-CoV-2 (strain USA-WA1/2020 isolate; BEI Resources, catalog no. NR-52281) was amplified in Vero E6 cells. .. Amplified stock virus was stored at −80°C until use.

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    BEI Resources vero e6 cells
    10× images of MDM, <t>Vero</t> E6, and Huh-7 cells taken 10 days following exposure to select volumes of normal semen, breast milk, or cell culture media (control). Breast milk was toxic at 50 and 100 µL/T-25 flask on Vero <t>E6</t> cells (no cells present after 10 days) and MDM cells, but toxicity was less on these cells than on Huh7 cells.
    Vero E6 Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero e6 cells/product/BEI Resources
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero e6 cells - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    BEI Resources vero cells
    Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with <t>ZIKV</t> (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) <t>Vero</t> cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.
    Vero Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vero cells/product/BEI Resources
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vero cells - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    10× images of MDM, Vero E6, and Huh-7 cells taken 10 days following exposure to select volumes of normal semen, breast milk, or cell culture media (control). Breast milk was toxic at 50 and 100 µL/T-25 flask on Vero E6 cells (no cells present after 10 days) and MDM cells, but toxicity was less on these cells than on Huh7 cells.

    Journal: Viruses

    Article Title: Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice

    doi: 10.3390/v11020161

    Figure Lengend Snippet: 10× images of MDM, Vero E6, and Huh-7 cells taken 10 days following exposure to select volumes of normal semen, breast milk, or cell culture media (control). Breast milk was toxic at 50 and 100 µL/T-25 flask on Vero E6 cells (no cells present after 10 days) and MDM cells, but toxicity was less on these cells than on Huh7 cells.

    Article Snippet: This stock was passaged three times in Vero E6 cells (obtained through BEI Resources, NIAID, NIH: VERO C1008 (E6), Kidney (African green monkey), Working Cell Bank, NR-596) in MEM-α, L-alanyl-L-glutamine without nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified HI-FBS.

    Techniques: Cell Culture

    Neutralizing antibody titers in serum from mice receiving two (wk 6) or three (wk 10) immunizations mice were determined using microneutralization assays against the WT SARS-CoV-2 (2019-nCoV/USA-WA1/2020), pseudotyped lentivirus expressing the WT SARS-CoV-2 spike protein (Lenti-CoV2), and MA SARS-CoV-2. Viral neutralization was plotted as percentage inhibition of viral infection in Vero E6 cells (for WT virus and MA-virus) relative to virus only (no serum) positive controls versus the inverse serum dilution. The titer at which 50% inhibition of infection was achieved (IC50) was determined for the (A, B) WT virus and the (D, E) MA virus. (C) The results were confirmed for the same week 10 serum samples using the Lenti-CoV2 pseudovirus expressing firefly luciferase with HEK-293T cells expressing hACE2. Microneutralization titers using the Lenti-CoV2 were determined by detecting viral infection by measuring luminescence (*p

    Journal: bioRxiv

    Article Title: A Combination Adjuvant for the Induction of Potent Antiviral Immune Responses for a Recombinant SARS-CoV-2 Protein Vaccine

    doi: 10.1101/2021.02.18.431484

    Figure Lengend Snippet: Neutralizing antibody titers in serum from mice receiving two (wk 6) or three (wk 10) immunizations mice were determined using microneutralization assays against the WT SARS-CoV-2 (2019-nCoV/USA-WA1/2020), pseudotyped lentivirus expressing the WT SARS-CoV-2 spike protein (Lenti-CoV2), and MA SARS-CoV-2. Viral neutralization was plotted as percentage inhibition of viral infection in Vero E6 cells (for WT virus and MA-virus) relative to virus only (no serum) positive controls versus the inverse serum dilution. The titer at which 50% inhibition of infection was achieved (IC50) was determined for the (A, B) WT virus and the (D, E) MA virus. (C) The results were confirmed for the same week 10 serum samples using the Lenti-CoV2 pseudovirus expressing firefly luciferase with HEK-293T cells expressing hACE2. Microneutralization titers using the Lenti-CoV2 were determined by detecting viral infection by measuring luminescence (*p

    Article Snippet: Viruses WT SARS-CoV-2: SARS-CoV-2 clinical isolate USA-WA1/2020 (BEI resources; NR-52281), was propagated by culture in Vero E6 cells as previously described (ref).

    Techniques: Mouse Assay, Expressing, Neutralization, Inhibition, Infection, Luciferase

    Cellular antiviral activity of Jun9-53-2 and Jun9-72-2 against SARS-CoV-2 in Caco-2 hACE2 and Vero E6 cells. ( A ) Antiviral activity of GRL0617. ( B ) Antiviral activity of Jun9-53-2. ( C ) Antiviral activity of Jun9-72-2.

    Journal: bioRxiv

    Article Title: Discovery of SARS-CoV-2 papain-like protease inhibitors through a combination of high-throughput screening and FlipGFP-based reporter assay

    doi: 10.1101/2021.03.15.435551

    Figure Lengend Snippet: Cellular antiviral activity of Jun9-53-2 and Jun9-72-2 against SARS-CoV-2 in Caco-2 hACE2 and Vero E6 cells. ( A ) Antiviral activity of GRL0617. ( B ) Antiviral activity of Jun9-53-2. ( C ) Antiviral activity of Jun9-72-2.

    Article Snippet: SARS-CoV-2, isolate USA-WA1/2020 (NR-52281), was obtained through BEI Resources and propagated once on VERO E6 cells before it was used for this study.

    Techniques: Activity Assay

    Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.

    Journal: Journal of Virology

    Article Title: Polyamine Depletion Inhibits Bunyavirus Infection via Generation of Noninfectious Interfering Virions

    doi: 10.1128/JVI.00530-19

    Figure Lengend Snippet: Polyamines enhance specific infectivity of enveloped viruses. (A to C) Huh7 cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with ZIKV (strain MR766) at an MOI of 0.1 PFU per cell. At 48 hpi, viral titers were determined via plaque assay (A) and relative viral genomes were determined by qRT-PCR (B), and the ratio of genomes to PFU was calculated (C). (D to F) Vero cells were treated with 500 μM or 1 mM DFMO for 4 days prior to infection with CVB3 (Nancy strain) at an MOI of 0.1 PFU per cell. At 24 hpi, viral titers were determined via plaque assay (D) and relative viral genomes were determined by qRT-PCR (E), and the ratio of genomes to PFU was calculated (F). (G to I) Identical infection and analysis methods were performed for KEYV in Huh7 cells to determine viral titers (G), viral genomes (H), and genome-to-PFU ratio (I) at 48 hpi. (J to L) These methods were also used for VSV (strain Indiana) infected at an MOI of 0.01 PFU per cell for 24 h. Viral titers (J), genomes (K), and genome-to-PFU ratio (L) were calculated. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (using Student’s t test [ n ≥ 3]). Error bars represent ±1 SEM. Statistical comparisons were performed between treated and untreated conditions.

    Article Snippet: ZIKV (MR766) was derived from the first passage of virus in Vero cells.

    Techniques: Infection, Plaque Assay, Quantitative RT-PCR