vero e6 cells  (ATCC)


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    ATCC vero e6 cells
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    vero e6 cells  (ATCC)


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    ATCC vero e6 cells
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    vero c1008 cells  (ATCC)


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    ATCC vero c1008 cells
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    vero cell lines  (ATCC)


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    ATCC vero cell lines
    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to <t>Vero</t> cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.
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    1) Product Images from "CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation"

    Article Title: CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation

    Journal: Nature Communications

    doi: 10.1038/s41467-024-48288-z

    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.
    Figure Legend Snippet: A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.

    Techniques Used: Real-time Polymerase Chain Reaction, Infection, Enzyme-linked Immunosorbent Assay, Fluorescence, Microscopy, Injection, Two Tailed Test

    A THP-1 cells were infected with VSV-GFP at an MOI of 1 for 12 h in the presence or absence of TG003 (20 µM) before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. B THP-1 cells were infected with VSV-GFP at an MOI of 1 for 1 h in the presence or absence of TG003 (20 µM) before the medium was replaced, and the cells were cultured for 24 h. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 3). C Real-time PCR analysis of IFNB1 and RANTES mRNA levels in the presence or absence of TG003 in THP-1 cells infected with SeV for 12 h ( n = 3). D TG003 alleviated VSV-induced conjunctivitis at 10 mg/kg or 20 mg/kg. E – G The effects of TG003 on the antiviral response in vivo. 6-week-old female mice ( n = 6) were infected with VSV (2 × 10 7 pfu/g), after which DMSO or 10 mg/kg or 20 mg/kg TG003 was intraperitoneally injected three times each day. E Mouse survival (Kaplan‒Meier curve) was monitored for 15 days. F Serum samples were collected after 24 h and subjected to plaque assays ( n = 6) and ( G ) ELISA analysis of Ifnb1 and Rantes ( n = 5). H H&E staining and ear thickness in IMQ-treated mice on Day 6 ( n = 6). I , J Real-time PCR analysis of Il-17a and Il-17f mRNA levels after treatment of Clk2-deficient mice with IMQ for 6 days or after intraperitoneal injection of TG003 ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( B , F ) or two-tailed ANOVA ( C , G – J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Source data ( A – J ) are provided as a Source Data file.
    Figure Legend Snippet: A THP-1 cells were infected with VSV-GFP at an MOI of 1 for 12 h in the presence or absence of TG003 (20 µM) before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. B THP-1 cells were infected with VSV-GFP at an MOI of 1 for 1 h in the presence or absence of TG003 (20 µM) before the medium was replaced, and the cells were cultured for 24 h. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 3). C Real-time PCR analysis of IFNB1 and RANTES mRNA levels in the presence or absence of TG003 in THP-1 cells infected with SeV for 12 h ( n = 3). D TG003 alleviated VSV-induced conjunctivitis at 10 mg/kg or 20 mg/kg. E – G The effects of TG003 on the antiviral response in vivo. 6-week-old female mice ( n = 6) were infected with VSV (2 × 10 7 pfu/g), after which DMSO or 10 mg/kg or 20 mg/kg TG003 was intraperitoneally injected three times each day. E Mouse survival (Kaplan‒Meier curve) was monitored for 15 days. F Serum samples were collected after 24 h and subjected to plaque assays ( n = 6) and ( G ) ELISA analysis of Ifnb1 and Rantes ( n = 5). H H&E staining and ear thickness in IMQ-treated mice on Day 6 ( n = 6). I , J Real-time PCR analysis of Il-17a and Il-17f mRNA levels after treatment of Clk2-deficient mice with IMQ for 6 days or after intraperitoneal injection of TG003 ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( B , F ) or two-tailed ANOVA ( C , G – J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Source data ( A – J ) are provided as a Source Data file.

    Techniques Used: Infection, Fluorescence, Microscopy, Cell Culture, Real-time Polymerase Chain Reaction, In Vivo, Injection, Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test

    vero cells  (ATCC)


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    vero cells  (ATCC)


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    vero cells  (ATCC)


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    vero cells  (ATCC)


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    vero c1008 cells  (ATCC)


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    ATCC vero c1008 cells
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    vero cell line  (ATCC)


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    ATCC vero cell lines
    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to <t>Vero</t> cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.
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    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to <t>Vero</t> cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.
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    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to <t>Vero</t> cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.
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    A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation

    doi: 10.1038/s41467-024-48288-z

    Figure Lengend Snippet: A Real-time PCR analysis of Ifnb1 and Rantes mRN levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). B ELISA analysis of Ifnb1 and Rantes protein levels in Clk2 +/+ and Clk2 −/− BMDMs and MLFs infected with SeV for 12 h or HSV-1 for 6 h ( n = 3). C Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 12 h before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. D Clk2 +/+ and Clk2 −/− BMDMs and MLFs were infected with VSV-GFP at an MOI of 2 for 1 h, followed by culture for 24 h with fresh medium. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 2). E Survival assays (Kaplan‒Meier curves) of wild-type and Clk2 −/− female mice at 6 weeks after infection with wild-type VSV (2 × 10 7 pfu/g) via the tail vein and intraperitoneal injection. Mouse survival was monitored for 15 days ( n = 6). F ELISA analysis of Ifnb1 and Rantes protein levels in the sera of VSV-infected Clk2 +/+ and Clk2 −/− female mice at 24 h ( n = 6). G Plaque assays in VSV-infected Clk2 +/+ and Clk2 −/− mouse sera ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( D , F , G ) or two-tailed ANOVA ( A , B ) ( p > 0.05, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001). Source data ( A – G ) are provided as a Source Data file.

    Article Snippet: HEK293T, HeLa, THP-1 and Vero cell lines were originally obtained from the ATCC.

    Techniques: Real-time Polymerase Chain Reaction, Infection, Enzyme-linked Immunosorbent Assay, Fluorescence, Microscopy, Injection, Two Tailed Test

    A THP-1 cells were infected with VSV-GFP at an MOI of 1 for 12 h in the presence or absence of TG003 (20 µM) before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. B THP-1 cells were infected with VSV-GFP at an MOI of 1 for 1 h in the presence or absence of TG003 (20 µM) before the medium was replaced, and the cells were cultured for 24 h. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 3). C Real-time PCR analysis of IFNB1 and RANTES mRNA levels in the presence or absence of TG003 in THP-1 cells infected with SeV for 12 h ( n = 3). D TG003 alleviated VSV-induced conjunctivitis at 10 mg/kg or 20 mg/kg. E – G The effects of TG003 on the antiviral response in vivo. 6-week-old female mice ( n = 6) were infected with VSV (2 × 10 7 pfu/g), after which DMSO or 10 mg/kg or 20 mg/kg TG003 was intraperitoneally injected three times each day. E Mouse survival (Kaplan‒Meier curve) was monitored for 15 days. F Serum samples were collected after 24 h and subjected to plaque assays ( n = 6) and ( G ) ELISA analysis of Ifnb1 and Rantes ( n = 5). H H&E staining and ear thickness in IMQ-treated mice on Day 6 ( n = 6). I , J Real-time PCR analysis of Il-17a and Il-17f mRNA levels after treatment of Clk2-deficient mice with IMQ for 6 days or after intraperitoneal injection of TG003 ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( B , F ) or two-tailed ANOVA ( C , G – J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Source data ( A – J ) are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation

    doi: 10.1038/s41467-024-48288-z

    Figure Lengend Snippet: A THP-1 cells were infected with VSV-GFP at an MOI of 1 for 12 h in the presence or absence of TG003 (20 µM) before phase contrast and fluorescence microscopy and flow cytometric analysis. The scale bar is 250 µm. B THP-1 cells were infected with VSV-GFP at an MOI of 1 for 1 h in the presence or absence of TG003 (20 µM) before the medium was replaced, and the cells were cultured for 24 h. Then, the supernatants were diluted and added to Vero cells for plaque assays ( n = 3). C Real-time PCR analysis of IFNB1 and RANTES mRNA levels in the presence or absence of TG003 in THP-1 cells infected with SeV for 12 h ( n = 3). D TG003 alleviated VSV-induced conjunctivitis at 10 mg/kg or 20 mg/kg. E – G The effects of TG003 on the antiviral response in vivo. 6-week-old female mice ( n = 6) were infected with VSV (2 × 10 7 pfu/g), after which DMSO or 10 mg/kg or 20 mg/kg TG003 was intraperitoneally injected three times each day. E Mouse survival (Kaplan‒Meier curve) was monitored for 15 days. F Serum samples were collected after 24 h and subjected to plaque assays ( n = 6) and ( G ) ELISA analysis of Ifnb1 and Rantes ( n = 5). H H&E staining and ear thickness in IMQ-treated mice on Day 6 ( n = 6). I , J Real-time PCR analysis of Il-17a and Il-17f mRNA levels after treatment of Clk2-deficient mice with IMQ for 6 days or after intraperitoneal injection of TG003 ( n = 6). The data are representative of three independent experiments. The data are presented as the means ± SEMs. Statistical significance was analyzed by two-tailed Student’s t test ( B , F ) or two-tailed ANOVA ( C , G – J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Source data ( A – J ) are provided as a Source Data file.

    Article Snippet: HEK293T, HeLa, THP-1 and Vero cell lines were originally obtained from the ATCC.

    Techniques: Infection, Fluorescence, Microscopy, Cell Culture, Real-time Polymerase Chain Reaction, In Vivo, Injection, Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test