vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of <t>VERO-CCL81</t> cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Development of a Rabies Virus-Vectored Vaccine against Borrelia burgdorferi , Targeting BBI39"

    Article Title: The Development of a Rabies Virus-Vectored Vaccine against Borrelia burgdorferi , Targeting BBI39

    Journal: Vaccines

    doi: 10.3390/vaccines12010078

    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of VERO-CCL81 cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).
    Figure Legend Snippet: RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of VERO-CCL81 cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).

    Techniques Used: Immunofluorescence, Western Blot, Infection, Staining, Purification, Produced

    vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero ccl81 cell monolayers  (ATCC)


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    ATCC vero ccl81 cell monolayers
    Vero Ccl81 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    Medium-throughput screening pipeline to identify antiviral compounds against St. Louis encephalitis virus infection. (1) The MMV COVID Box library, which comprises eighty compounds with known or predicted activity against SARS-CoV-2, was screened at 20 µM using a phenotypical assay of SLEV infection in cell culture. Briefly, hit compounds were selected based on their ability to reduce the CPE induced by SLEV infection in Vero <t>CCL81</t> cells, which was quantified by a high-content fluorescence microscope detecting Hoechst-stained nuclei in cell cultures. Nine hit compounds were selected due to inhibition of at least 40% of the CPE induced by viral infection at 72 h post-infection. (2) Six compounds were resupplied for the confirmatory assay. Dose–response curves were created for Vero CCL81 cell cultures for the calculation of EC 50 and CC 50 values. (3) Hit validation was performed using Vero and the human cell lines HBEC-5i and HUH-7. The antiviral activity was confirmed by plaque-forming assays. (4) The pipeline led to the identification of Triparanol and Fluphenazine with antiviral activity against SLEV infection.
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Phenotypical Screening of an MMV Open Box Library and Identification of Compounds with Antiviral Activity against St. Louis Encephalitis Virus"

    Article Title: Phenotypical Screening of an MMV Open Box Library and Identification of Compounds with Antiviral Activity against St. Louis Encephalitis Virus

    Journal: Viruses

    doi: 10.3390/v15122416

    Medium-throughput screening pipeline to identify antiviral compounds against St. Louis encephalitis virus infection. (1) The MMV COVID Box library, which comprises eighty compounds with known or predicted activity against SARS-CoV-2, was screened at 20 µM using a phenotypical assay of SLEV infection in cell culture. Briefly, hit compounds were selected based on their ability to reduce the CPE induced by SLEV infection in Vero CCL81 cells, which was quantified by a high-content fluorescence microscope detecting Hoechst-stained nuclei in cell cultures. Nine hit compounds were selected due to inhibition of at least 40% of the CPE induced by viral infection at 72 h post-infection. (2) Six compounds were resupplied for the confirmatory assay. Dose–response curves were created for Vero CCL81 cell cultures for the calculation of EC 50 and CC 50 values. (3) Hit validation was performed using Vero and the human cell lines HBEC-5i and HUH-7. The antiviral activity was confirmed by plaque-forming assays. (4) The pipeline led to the identification of Triparanol and Fluphenazine with antiviral activity against SLEV infection.
    Figure Legend Snippet: Medium-throughput screening pipeline to identify antiviral compounds against St. Louis encephalitis virus infection. (1) The MMV COVID Box library, which comprises eighty compounds with known or predicted activity against SARS-CoV-2, was screened at 20 µM using a phenotypical assay of SLEV infection in cell culture. Briefly, hit compounds were selected based on their ability to reduce the CPE induced by SLEV infection in Vero CCL81 cells, which was quantified by a high-content fluorescence microscope detecting Hoechst-stained nuclei in cell cultures. Nine hit compounds were selected due to inhibition of at least 40% of the CPE induced by viral infection at 72 h post-infection. (2) Six compounds were resupplied for the confirmatory assay. Dose–response curves were created for Vero CCL81 cell cultures for the calculation of EC 50 and CC 50 values. (3) Hit validation was performed using Vero and the human cell lines HBEC-5i and HUH-7. The antiviral activity was confirmed by plaque-forming assays. (4) The pipeline led to the identification of Triparanol and Fluphenazine with antiviral activity against SLEV infection.

    Techniques Used: Virus, Infection, Activity Assay, Cell Culture, Fluorescence, Microscopy, Staining, Inhibition, Confirmatory Assay

    Hit compound validation of antiviral activity against SLEV in Vero CCL81 cells. ( A ) Compound cytotoxicity was assessed through MTT assay. ( B ) Viral load measured by plaque assay. ( C ) Plaque assays of the most promising hits in Vero cells. A serial dilution ranging from 10 −1 to 10 −6 was performed on each sample. The antiviral activity of compounds promoted the reduction in lysis plates in comparison to positive control (vehicle-treated group). ** p < 0.01, **** p < 0.0001 relative to the virus-infected, vehicle-treated control group. Data represent two independent experiments ( n = 6).
    Figure Legend Snippet: Hit compound validation of antiviral activity against SLEV in Vero CCL81 cells. ( A ) Compound cytotoxicity was assessed through MTT assay. ( B ) Viral load measured by plaque assay. ( C ) Plaque assays of the most promising hits in Vero cells. A serial dilution ranging from 10 −1 to 10 −6 was performed on each sample. The antiviral activity of compounds promoted the reduction in lysis plates in comparison to positive control (vehicle-treated group). ** p < 0.01, **** p < 0.0001 relative to the virus-infected, vehicle-treated control group. Data represent two independent experiments ( n = 6).

    Techniques Used: Activity Assay, MTT Assay, Plaque Assay, Serial Dilution, Lysis, Comparison, Positive Control, Virus, Infection

    vero ccl81 cells  (ATCC)


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    ATCC vero ccl81 cells
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero atcc ccl81 cells  (ATCC)


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    ATCC vero atcc ccl81 cells
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    vero atcc ccl81 cells  (ATCC)


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    ATCC vero atcc ccl81 cells
    Vero Atcc Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero atcc ccl81 cells  (ATCC)


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    ATCC vero atcc ccl81 cells
    Vero Atcc Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC vero ccl81 cells
    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of <t>VERO-CCL81</t> cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).
    Vero Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC vero ccl81 cell monolayers
    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of <t>VERO-CCL81</t> cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).
    Vero Ccl81 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC vero atcc ccl81 cells
    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of <t>VERO-CCL81</t> cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).
    Vero Atcc Ccl81 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of VERO-CCL81 cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).

    Journal: Vaccines

    Article Title: The Development of a Rabies Virus-Vectored Vaccine against Borrelia burgdorferi , Targeting BBI39

    doi: 10.3390/vaccines12010078

    Figure Lengend Snippet: RABV can incorporate the chimeric borrelial protein BBI39 RVG . Characterization of RABV-vectored vaccine viruses via immunofluorescence ( a , c ) and Western blot ( b , d ). For immunofluorescence, VEROCCL81 cells were infected at an MOI of 0.05 for 72 h and fixed and permeabilized for intracellular ( a ) and fixed only for surface staining ( c ). Cells were stained with human anti-RABV-G (4C12) (green) and polyclonal mouse anti-BBI39 (magenta) and mounted with mounting media containing DAPI (blue). Images were taken at 40X magnification, and scale bars represent 10 μm. Western blot of cell lysates ( b ) of VERO-CCL81 cells infected at an MOI of 0.05 for 72 h that were probed for BBI39 and RABV-G. Western blots of sucrose-purified virions ( d ) that were probed for BBI39 and RABV-G. Blots were probed with either polyclonal mouse-anti-BBI39 (produced by the Pal lab at University of Maryland) or 4C12 human anti-RABV-G monoclonal antibody (provided by Scott Dessain, Lankenau Institute for Medical Research, Wynnewood, PA) ( b , d ).

    Article Snippet: BSR and Vero (CCL81) cells were obtained from ATCC and cultured in 1X DMEM (Corning Cat# 10-013-CV, Corning, NY, USA) supplemented with 5% fetal bovine serum (FBS) and 1% penicillin-streptomycin.

    Techniques: Immunofluorescence, Western Blot, Infection, Staining, Purification, Produced