veriti 96 well thermal cycler  (Thermo Fisher)


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    Veriti 96 Well Thermal Cycler
    Description:
    The Applied Biosystems Veriti 96 Well Thermal Cycler delivers the proven reliability of Applied Biosystems instruments With the added control of VeriFlex technology you have six independent temperature blocks that provide precise control over your PCR optimization Additionally the color touch screen simplifies setup and use of the Veriti 96 Well Fast Thermal Cycler Optional setups for fast or standard PCR methods provide with flexibility to shorten your PCR cycling times The Veriti 96 Well Thermal Cycler is equipped with a standard 0 2 mL block configuration Click here to Request a Demo Key Highlights • Innovative VeriFlex Blocks allow for precise PCR optimization and enable you to run up to 6 independent assays • Standard 0 2 mL block format is compatible with many downstream applications • Standard and fast enabled cycling to address your current and future PCR needs • Easy to use graphical interface 6 5 inch VGA Touch Screen • Convenient protocol transfer from one Veriti Thermal Cycler to another using USB • Reduced PCR cycling time when using faster ramp rates • Optional VeritiLink Remote Management Software provides you with peace of mind allowing you to remotely manage more than 50 cyclers from your PC or internet compatible phone Extended Warranty Packages Protect your instrument and your investment with Veriti Thermal Cycler Extended Warranty Packages and save up to 25 off the individual instrument and extended warranty list prices Learn more VeriFlex Blocks Enhanced PCR Functionality The Veriti 96 Well Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality Six separate peltier blocks provide maximal versatility and flexibility and offer two key benefits 1 PCR Optimization each block can be set with a specific temperature which is ideal for precise control over PCR optimization 2 Run more experiments the six peltier blocks can also be used to run up to six different annealing temperatures in the same run With this enhanced functionality the Veriti 96 Well Thermal Cycler with VeriFlex blocks is the ideal thermal cycler for any lab Control at your fingertips The powerful yet simple to operate user interface on the Veriti system is driven by the 6 5 inch 16 51 cm VGA color touch screen The large screen allows for easy viewing of your temperature profiles Additionally the large navigation buttons put programming of the Veriti Thermal Cycler at your finger tips Setup and navigation of the Veriti Thermal Cycler does not require the use of a stylus or mouse Fast setup fast results The Veriti system has two different options for method navigation For quick setup you may select one of the pre programmed methods to run If you prefer to program your own methods simply touch the step you would like to setup and then use the keypad to enter thermal cycling steps After completing a setup you have the choice of saving the method to the instrument or to a USB memory stick Control your time Go fast when you want With the Veriti Thermal Cycler you get two thermal cyclers in one a standard thermal cycler with its better than gradient feature and a fast enabled thermal cycler So whether you have a current need to run fast PCR and shorten your PCR cycling times or a possible future application need for fast PCR the Veriti Thermal Cycler provides you with the flexibility to go fast when you want For Research Use Only Not for use in diagnostic procedures
    Catalog Number:
    4375786
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    Fast PCR|PCR|PCR & Real-Time PCR|Routine PCR|Thermal Cyclers
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    Structured Review

    Thermo Fisher veriti 96 well thermal cycler
    The Applied Biosystems Veriti 96 Well Thermal Cycler delivers the proven reliability of Applied Biosystems instruments With the added control of VeriFlex technology you have six independent temperature blocks that provide precise control over your PCR optimization Additionally the color touch screen simplifies setup and use of the Veriti 96 Well Fast Thermal Cycler Optional setups for fast or standard PCR methods provide with flexibility to shorten your PCR cycling times The Veriti 96 Well Thermal Cycler is equipped with a standard 0 2 mL block configuration Click here to Request a Demo Key Highlights • Innovative VeriFlex Blocks allow for precise PCR optimization and enable you to run up to 6 independent assays • Standard 0 2 mL block format is compatible with many downstream applications • Standard and fast enabled cycling to address your current and future PCR needs • Easy to use graphical interface 6 5 inch VGA Touch Screen • Convenient protocol transfer from one Veriti Thermal Cycler to another using USB • Reduced PCR cycling time when using faster ramp rates • Optional VeritiLink Remote Management Software provides you with peace of mind allowing you to remotely manage more than 50 cyclers from your PC or internet compatible phone Extended Warranty Packages Protect your instrument and your investment with Veriti Thermal Cycler Extended Warranty Packages and save up to 25 off the individual instrument and extended warranty list prices Learn more VeriFlex Blocks Enhanced PCR Functionality The Veriti 96 Well Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality Six separate peltier blocks provide maximal versatility and flexibility and offer two key benefits 1 PCR Optimization each block can be set with a specific temperature which is ideal for precise control over PCR optimization 2 Run more experiments the six peltier blocks can also be used to run up to six different annealing temperatures in the same run With this enhanced functionality the Veriti 96 Well Thermal Cycler with VeriFlex blocks is the ideal thermal cycler for any lab Control at your fingertips The powerful yet simple to operate user interface on the Veriti system is driven by the 6 5 inch 16 51 cm VGA color touch screen The large screen allows for easy viewing of your temperature profiles Additionally the large navigation buttons put programming of the Veriti Thermal Cycler at your finger tips Setup and navigation of the Veriti Thermal Cycler does not require the use of a stylus or mouse Fast setup fast results The Veriti system has two different options for method navigation For quick setup you may select one of the pre programmed methods to run If you prefer to program your own methods simply touch the step you would like to setup and then use the keypad to enter thermal cycling steps After completing a setup you have the choice of saving the method to the instrument or to a USB memory stick Control your time Go fast when you want With the Veriti Thermal Cycler you get two thermal cyclers in one a standard thermal cycler with its better than gradient feature and a fast enabled thermal cycler So whether you have a current need to run fast PCR and shorten your PCR cycling times or a possible future application need for fast PCR the Veriti Thermal Cycler provides you with the flexibility to go fast when you want For Research Use Only Not for use in diagnostic procedures
    https://www.bioz.com/result/veriti 96 well thermal cycler/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    veriti 96 well thermal cycler - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Endothelial progenitor cells display clonal restriction in multiple myeloma
    Article Snippet: Reactions performed in duplicate for IGH quantitation contained 10 ng of either EPC or BM cell genomic DNA from Case 15, and 2 × qPCR master mix containing 8 μM of either IGH primers (VH 4 forward 5'-TCGGAGACCCTGTCCCTCACCTGCA-3', patient allele-specific reverse 5'-CCAGAGATCGAAGAACCAGTCTTC-3') or ApoB primers (ApoB forward 5'-GCAAG CAGAA GCCAG AAGTG A-3', ApoB reverse 5'-CCATT TGGAG AAGCAGTTTG G -3'). .. Amplification conditions, using an ABI Prism 7000 sequence detector equipped with a 96-well thermal cycler (Applied Biosystems, Foster City, CA), were 10 minutes at 95°C (to activate the enzyme) and 40 cyclesof denaturation at 95°C for 15 seconds and annealing/extension at 60°C for 1 minute. .. Data were collected and analyzed with ABI Prism SDS software v1.0 (Applied Biosystems, Foster City, CA).

    Sequencing:

    Article Title: Endothelial progenitor cells display clonal restriction in multiple myeloma
    Article Snippet: Reactions performed in duplicate for IGH quantitation contained 10 ng of either EPC or BM cell genomic DNA from Case 15, and 2 × qPCR master mix containing 8 μM of either IGH primers (VH 4 forward 5'-TCGGAGACCCTGTCCCTCACCTGCA-3', patient allele-specific reverse 5'-CCAGAGATCGAAGAACCAGTCTTC-3') or ApoB primers (ApoB forward 5'-GCAAG CAGAA GCCAG AAGTG A-3', ApoB reverse 5'-CCATT TGGAG AAGCAGTTTG G -3'). .. Amplification conditions, using an ABI Prism 7000 sequence detector equipped with a 96-well thermal cycler (Applied Biosystems, Foster City, CA), were 10 minutes at 95°C (to activate the enzyme) and 40 cyclesof denaturation at 95°C for 15 seconds and annealing/extension at 60°C for 1 minute. .. Data were collected and analyzed with ABI Prism SDS software v1.0 (Applied Biosystems, Foster City, CA).

    Polymerase Chain Reaction:

    Article Title: Simultaneous Detection of Oseltamivir- and Amantadine-Resistant Influenza by Oligonucleotide Microarray Visualization
    Article Snippet: For the oseltamivir-resistant mutation fragment amplification, the RT-PCR system contained each reverse primer at 0.5 µM and each forward primer at 0.1 µM; while for the amantadine-resistant mutation fragment amplification, it contained each reverse primer at 0.75 µM and each forward primer at 0.15 µM. .. Amplifications were performed on a Veritil 96-Well Thermal Cycler PCR system (Applied Biosystems) using the following conditions: 30 min at 50°C; 2 min at 94°C; 45 cycles of 20 s at 94°C, 20 s at 55°C, and 20 s at 72°C; and a final extension of 5 min at 72°C. .. Hybridization and signal detection After the resistance mutation fragments were amplified, 2.5 µL of each amplification product of the two reactions was mixed with 5 µL of hybridization buffer (8×SSC, 0.6% SDS, 10% formylamine, and 10×Denhardt).

    Article Title: Association of OGG1 and DLST promoter methylation with Alzheimer's disease in Xinjiang population
    Article Snippet: Polymerase chain reaction (PCR) was carried out in 40 µl volume containing 2 µl of each primer, 4 µl genomic DNA, 12 µl ddH2O and 20 µl 2X HotTaq Master Mix. .. PCR was performed in a Veriti 96-well thermal cycler (Applied Biosystems; Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. Genotyping was done using the Sanger sequencing.

    Article Title: Masticatory muscles of mouse do not undergo atrophy in space
    Article Snippet: Reverse transcription was performed on 1 μg total RNA from each muscle sample (Applied Biosystems, Foster City, CA, USA), and the resultant cDNA was used in real-time PCR. .. Real-time PCR analyses were performed using Applied Biosystems 96-well Thermal Cyclers (Applied Biosystems), 7300 Real-Time PCR System, Step One Plus PCR System, and reagents (Power SYBRgreen PCR Master mix). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Masticatory muscles of mouse do not undergo atrophy in space
    Article Snippet: Reverse transcription was performed on 1 μg total RNA from each muscle sample (Applied Biosystems, Foster City, CA, USA), and the resultant cDNA was used in real-time PCR. .. Real-time PCR analyses were performed using Applied Biosystems 96-well Thermal Cyclers (Applied Biosystems), 7300 Real-Time PCR System, Step One Plus PCR System, and reagents (Power SYBRgreen PCR Master mix). ..

    Article Title: Low-dose anti-inflammatory combinatorial therapy reduced cancer stem cell formation in patient-derived preclinical models for tumour relapse prevention
    Article Snippet: In all, 1 × first-strand buffer, 5 mM DTT, 40 U RNaseOUT Recombinant RNase Inhibitor, 200 U SuperScript III RT (all Life Technologies) were added to a final volume of 20 μl. .. The following thermal setting was applied on a Verity 96-well Thermal Cycler (Applied Biosystems): 25 °C for 5 min, 55 °C for 60 min and 85 °C for 5 min. Each RT product was diluted to a final volume of 40 μl to avoid qPCR inhibition. .. Real-time quantitative PCR (RT-qPCR) RT-qPCR was carried out in real-time using SYBR Green I detection chemistry on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories).

    Inhibition:

    Article Title: Low-dose anti-inflammatory combinatorial therapy reduced cancer stem cell formation in patient-derived preclinical models for tumour relapse prevention
    Article Snippet: In all, 1 × first-strand buffer, 5 mM DTT, 40 U RNaseOUT Recombinant RNase Inhibitor, 200 U SuperScript III RT (all Life Technologies) were added to a final volume of 20 μl. .. The following thermal setting was applied on a Verity 96-well Thermal Cycler (Applied Biosystems): 25 °C for 5 min, 55 °C for 60 min and 85 °C for 5 min. Each RT product was diluted to a final volume of 40 μl to avoid qPCR inhibition. .. Real-time quantitative PCR (RT-qPCR) RT-qPCR was carried out in real-time using SYBR Green I detection chemistry on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories).

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    • 96 well thermal cycler  (Thermo Fisher)
    99
    Thermo Fisher 96 well thermal cycler
    Characterization of confluent EPC cultures expanded from BM expressing KDR, VE-cadherin, CD133, and vWF . (A) Colony-forming and outgrowing endothelial cells from a representative patient are shown. A colony-forming unit expressing: (1) KDR (red), (2) VE-cadherin (green), and (3) merge shows independent cellular distribution of KDR and VE-cadherin. Outgrowing EPCs expressing: (4) KDR (green), (5) early hematopoietic antigen CD133 (red), and (6) merge show independent cellular localization of KDR and CD133. Cultures were maintained on <t>96-well,</t> laminin-coated plates, where colony growth and confluent EPCs were observed after 14 and 18 days of culture, respectively. Indirect immunofluorescence was done with indicated antibodies, and appropriate isotype-matched serum with each of the primary antibodies was used as negative control (not shown). Images of the stained cells were digitally recorded on a confocal laser scanning microscope (Bio-Rad MRC 1024ES; Bio-Rad, Hercules, CA) and were generated at the projections of the z-stacks at 1024 × 1024 pixels. (B) Co-staining of EPCs grown from BM for expression of vWF (green), CD38 (red), and nuclear counterstain TO-PRO-3 (blue). Data from a representative patient show that EPCs are vWF-positive. Boxes on the left indicate patterns of immunocytochemical staining of the same EPCs; 3-D histogram on the right shows 2-color FACS analysis using anti-vWF-FITC, anti-CD38-PE, and isotype-specific control antibodies. (C) Two-color dot plot of primary EPC culture from a representative patient using anti-vWF-FITC, anti-CD133-PE, and anti-CD45-PE/Cy5 antibodies. Quadrants were set based upon isotype-specific controls for FITC and PE (and PE/Cy5, not shown). Numbers shown in the quadrants reflect percent of EPCs from which small, agranular debris was gated out based on forward and side scatter plots.
    96 Well Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well thermal cycler/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    96 well thermal cycler - by Bioz Stars, 2021-03
    99/100 stars
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    quantitative real time polymerase chain reaction qrt pcr qrt pcr  (Thermo Fisher)
    93
    Thermo Fisher quantitative real time polymerase chain reaction qrt pcr qrt pcr
    Stability of miRNA in whole blood incubated at room temperature. Whole blood was collected into EDTA containing tubes and incubated for 0, 4, 8, 12, 24 and 72 h at room temperature before processed into plasma. <t>qRT-PCR</t> was performed. Statistically significant differences in miRNA expression are marked with asterisks (**). **p
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time polymerase chain reaction qrt pcr qrt pcr/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative real time polymerase chain reaction qrt pcr qrt pcr - by Bioz Stars, 2021-03
    93/100 stars
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    veriti fast thermal cycler  (Thermo Fisher)
    95
    Thermo Fisher veriti fast thermal cycler
    Stability of miRNA in whole blood incubated at room temperature. Whole blood was collected into EDTA containing tubes and incubated for 0, 4, 8, 12, 24 and 72 h at room temperature before processed into plasma. <t>qRT-PCR</t> was performed. Statistically significant differences in miRNA expression are marked with asterisks (**). **p
    Veriti Fast Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/veriti fast thermal cycler/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    veriti fast thermal cycler - by Bioz Stars, 2021-03
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of confluent EPC cultures expanded from BM expressing KDR, VE-cadherin, CD133, and vWF . (A) Colony-forming and outgrowing endothelial cells from a representative patient are shown. A colony-forming unit expressing: (1) KDR (red), (2) VE-cadherin (green), and (3) merge shows independent cellular distribution of KDR and VE-cadherin. Outgrowing EPCs expressing: (4) KDR (green), (5) early hematopoietic antigen CD133 (red), and (6) merge show independent cellular localization of KDR and CD133. Cultures were maintained on 96-well, laminin-coated plates, where colony growth and confluent EPCs were observed after 14 and 18 days of culture, respectively. Indirect immunofluorescence was done with indicated antibodies, and appropriate isotype-matched serum with each of the primary antibodies was used as negative control (not shown). Images of the stained cells were digitally recorded on a confocal laser scanning microscope (Bio-Rad MRC 1024ES; Bio-Rad, Hercules, CA) and were generated at the projections of the z-stacks at 1024 × 1024 pixels. (B) Co-staining of EPCs grown from BM for expression of vWF (green), CD38 (red), and nuclear counterstain TO-PRO-3 (blue). Data from a representative patient show that EPCs are vWF-positive. Boxes on the left indicate patterns of immunocytochemical staining of the same EPCs; 3-D histogram on the right shows 2-color FACS analysis using anti-vWF-FITC, anti-CD38-PE, and isotype-specific control antibodies. (C) Two-color dot plot of primary EPC culture from a representative patient using anti-vWF-FITC, anti-CD133-PE, and anti-CD45-PE/Cy5 antibodies. Quadrants were set based upon isotype-specific controls for FITC and PE (and PE/Cy5, not shown). Numbers shown in the quadrants reflect percent of EPCs from which small, agranular debris was gated out based on forward and side scatter plots.

    Journal: BMC Cancer

    Article Title: Endothelial progenitor cells display clonal restriction in multiple myeloma

    doi: 10.1186/1471-2407-6-161

    Figure Lengend Snippet: Characterization of confluent EPC cultures expanded from BM expressing KDR, VE-cadherin, CD133, and vWF . (A) Colony-forming and outgrowing endothelial cells from a representative patient are shown. A colony-forming unit expressing: (1) KDR (red), (2) VE-cadherin (green), and (3) merge shows independent cellular distribution of KDR and VE-cadherin. Outgrowing EPCs expressing: (4) KDR (green), (5) early hematopoietic antigen CD133 (red), and (6) merge show independent cellular localization of KDR and CD133. Cultures were maintained on 96-well, laminin-coated plates, where colony growth and confluent EPCs were observed after 14 and 18 days of culture, respectively. Indirect immunofluorescence was done with indicated antibodies, and appropriate isotype-matched serum with each of the primary antibodies was used as negative control (not shown). Images of the stained cells were digitally recorded on a confocal laser scanning microscope (Bio-Rad MRC 1024ES; Bio-Rad, Hercules, CA) and were generated at the projections of the z-stacks at 1024 × 1024 pixels. (B) Co-staining of EPCs grown from BM for expression of vWF (green), CD38 (red), and nuclear counterstain TO-PRO-3 (blue). Data from a representative patient show that EPCs are vWF-positive. Boxes on the left indicate patterns of immunocytochemical staining of the same EPCs; 3-D histogram on the right shows 2-color FACS analysis using anti-vWF-FITC, anti-CD38-PE, and isotype-specific control antibodies. (C) Two-color dot plot of primary EPC culture from a representative patient using anti-vWF-FITC, anti-CD133-PE, and anti-CD45-PE/Cy5 antibodies. Quadrants were set based upon isotype-specific controls for FITC and PE (and PE/Cy5, not shown). Numbers shown in the quadrants reflect percent of EPCs from which small, agranular debris was gated out based on forward and side scatter plots.

    Article Snippet: Amplification conditions, using an ABI Prism 7000 sequence detector equipped with a 96-well thermal cycler (Applied Biosystems, Foster City, CA), were 10 minutes at 95°C (to activate the enzyme) and 40 cyclesof denaturation at 95°C for 15 seconds and annealing/extension at 60°C for 1 minute.

    Techniques: Expressing, Immunofluorescence, Negative Control, Staining, Laser-Scanning Microscopy, Generated, FACS

    Stability of miRNA in whole blood incubated at room temperature. Whole blood was collected into EDTA containing tubes and incubated for 0, 4, 8, 12, 24 and 72 h at room temperature before processed into plasma. qRT-PCR was performed. Statistically significant differences in miRNA expression are marked with asterisks (**). **p

    Journal: PLoS ONE

    Article Title: Stability of Circulating Blood-Based MicroRNAs – Pre-Analytic Methodological Considerations

    doi: 10.1371/journal.pone.0167969

    Figure Lengend Snippet: Stability of miRNA in whole blood incubated at room temperature. Whole blood was collected into EDTA containing tubes and incubated for 0, 4, 8, 12, 24 and 72 h at room temperature before processed into plasma. qRT-PCR was performed. Statistically significant differences in miRNA expression are marked with asterisks (**). **p

    Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR) qRT-PCR was perfomed using TaqMan Gene Expression MasterMix (Life Technologies, USA) and TaqMan probes (Life Technologies, USA) for hsa-miR-1 (Assay-ID: 000385), hsa-miR-21 (Assay-ID:000397), hsa-miR-29b (Assay-ID: 000413) and cel-miR-39 (Assay-ID: 000200) according to manufacturer’s instructions under sterile conditions using either an Applied Biosystems 7300 Real-Time PCR System (Copenhagen samples) or a CFX 96 C1000 touch Realtime Cycler (Bio Rad, Germany) (Munich samples).

    Techniques: Incubation, Quantitative RT-PCR, Expressing