vent dna polymerase  (New England Biolabs)


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  • 99
    Name:
    Vent DNA Polymerase
    Description:
    Vent DNA Polymerase 1 000 units
    Catalog Number:
    m0254l
    Price:
    282
    Size:
    1 000 units
    Category:
    Thermostable DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs vent dna polymerase
    Vent DNA Polymerase
    Vent DNA Polymerase 1 000 units
    https://www.bioz.com/result/vent dna polymerase/product/New England Biolabs
    Average 99 stars, based on 270 article reviews
    Price from $9.99 to $1999.99
    vent dna polymerase - by Bioz Stars, 2020-01
    99/100 stars

    Images

    1) Product Images from "Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene"

    Article Title: Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.16-16-05026.1996

    Isolation of MAP1B promoter region using the linker-dependent genomic walking method. Primers and their relative positions are shown schematically in A . Rat genomic DNA was digested with a mixture of Bgl II, Xba I, and Bam HI, annealed with p4, extended with Klenow fragment, and then ligated with the unique linkers LMPCR1 and LMPCR2. First-round PCR amplification with primers p5 and LMPCR1 ( B ) and the corresponding Southern blot ( C ) show a smear with no clearly distinct bands in lane S . Second-round PCR amplification was performed using p6 and LMPCR1 as primers and first-round PCR products as templates ( D ) with the corresponding Southern blot ( E ). Lanes 1  and 2  in D and E show two distinct PCR products of 495 and 582 bp. M is a 100 bp DNA ladder.
    Figure Legend Snippet: Isolation of MAP1B promoter region using the linker-dependent genomic walking method. Primers and their relative positions are shown schematically in A . Rat genomic DNA was digested with a mixture of Bgl II, Xba I, and Bam HI, annealed with p4, extended with Klenow fragment, and then ligated with the unique linkers LMPCR1 and LMPCR2. First-round PCR amplification with primers p5 and LMPCR1 ( B ) and the corresponding Southern blot ( C ) show a smear with no clearly distinct bands in lane S . Second-round PCR amplification was performed using p6 and LMPCR1 as primers and first-round PCR products as templates ( D ) with the corresponding Southern blot ( E ). Lanes 1  and 2  in D and E show two distinct PCR products of 495 and 582 bp. M is a 100 bp DNA ladder.

    Techniques Used: Isolation, Polymerase Chain Reaction, Amplification, Southern Blot

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene
    Article Snippet: An alternative method, the PCR-based linker-dependent genomic walking procedure, was used simultaneously to isolate the MAP1B promoter region ( ). .. First-strand DNA was synthesized by annealing p4 with digested genomic DNA and extended with Vent DNA polymerase (New England Biolabs, Beverly, MA) at 95°C for 2 min, at 60°C for 30 min, and at 76°C for 10 min using a thermocycler.

    Clone Assay:

    Article Title: Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene
    Article Snippet: The three resulting clones, pBS-2.3R, pUC-2.5 Hr, and pUC-2.3X, were used for further analysis. .. First-strand DNA was synthesized by annealing p4 with digested genomic DNA and extended with Vent DNA polymerase (New England Biolabs, Beverly, MA) at 95°C for 2 min, at 60°C for 30 min, and at 76°C for 10 min using a thermocycler.

    Amplification:

    Article Title: Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene
    Article Snippet: First-strand DNA was synthesized by annealing p4 with digested genomic DNA and extended with Vent DNA polymerase (New England Biolabs, Beverly, MA) at 95°C for 2 min, at 60°C for 30 min, and at 76°C for 10 min using a thermocycler. .. PCR products were separated on a 1% agarose gel, transferred onto a nylon membrane, and hybridized with the radiolabeled 490 bp MAP1B cDNA probe.

    Synthesized:

    Article Title: Two Alternative Promoters Direct Neuron-Specific Expression of the Rat Microtubule-Associated Protein 1B Gene
    Article Snippet: .. First-strand DNA was synthesized by annealing p4 with digested genomic DNA and extended with Vent DNA polymerase (New England Biolabs, Beverly, MA) at 95°C for 2 min, at 60°C for 30 min, and at 76°C for 10 min using a thermocycler. ..

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