velos ltq mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher velos ltq mass spectrometer
    Velos Ltq Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/velos ltq mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    velos ltq mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars

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    Modification:

    Article Title: Ambient DESI and LESA-MS Analysis of Proteins Adsorbed to a Biomaterial Surface Using In-Situ Surface Tryptic Digestion
    Article Snippet: .. Detection of peptides was performed with the Prosolia DESI 2D Omnispray system (Prosolia, USA) with a slight modification to the MS inlet described previously [ ] on a Thermo Velos LTQ mass spectrometer (Thermo, USA). .. For limit of detection work the DESI parameters were setup as follows: DESI sprayer to surface angle 50°, DESI tip-to-surface distance 0.5 mm, DESI tip-to-MS inlet 2.5 mm, Velos MS inlet to surface ~0 mm, and Velos MS inlet to surface angle at 10°.

    Mass Spectrometry:

    Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux
    Article Snippet: .. Proteins were eluted from beads using 3× FLAG peptide, and samples were prepared and analyzed using a Velos LTQ mass spectrometer (Thermo Fisher Scientific) as described above for CompPASS analysis. ..

    Article Title: Ambient DESI and LESA-MS Analysis of Proteins Adsorbed to a Biomaterial Surface Using In-Situ Surface Tryptic Digestion
    Article Snippet: .. Detection of peptides was performed with the Prosolia DESI 2D Omnispray system (Prosolia, USA) with a slight modification to the MS inlet described previously [ ] on a Thermo Velos LTQ mass spectrometer (Thermo, USA). .. For limit of detection work the DESI parameters were setup as follows: DESI sprayer to surface angle 50°, DESI tip-to-surface distance 0.5 mm, DESI tip-to-MS inlet 2.5 mm, Velos MS inlet to surface ~0 mm, and Velos MS inlet to surface angle at 10°.

    Article Title: Short-Term Low Temperature Induces Nitro-Oxidative Stress that Deregulates the NADP-Malic Enzyme Function by Tyrosine Nitration in Arabidopsis thaliana
    Article Snippet: .. The resulting sample was subjected to a liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, using a Velos-LTQ mass spectrometer equipped with a micro-ESI ion source (Thermo Fisher, San Jose, CA, USA). ..

    Article Title: Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation
    Article Snippet: .. The sample was then analysed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a Velos-LTQ mass spectrometer equipped with a micro-ESI (electrospray ionization) ion source (ThermoFisher, San Jose, CA, USA). ..

    Article Title: Protein tyrosine nitration in pea roots during development and senescence
    Article Snippet: .. Then, sample was analysed by LC-MS/MS using a Velos-LTQ mass spectrometer equipped with a microESI ion source (ThermoFisher, San Jose, CA, USA). ..

    Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux
    Article Snippet: .. The resulting peptides were desalted on C18 Stage tips and analyzed using a Velos LTQ mass spectrometer (Thermo Fisher Scientific), followed by CompPASS analysis to identify high-confidence interacting proteins (HCIPs) ( , ). .. For size exclusion chromatography/AP-MS analysis, cells were lysed in lysis buffer as described above.

    Article Title: Histone H2B Ubiquitination Promotes the Function of the Anaphase-Promoting Complex/Cyclosome in Schizosaccharomyces pombe
    Article Snippet: .. Peptides were loaded onto 26-cm columns with a bomb pressure cell and then separated and analyzed by three-phase multidimensional protein identification technology on a Velos LTQ mass spectrometer (Thermo Scientific, West Palm Beach, FL) coupled to a nanoHPLC (NanoAcquity; Waters Corporation). ..

    Article Title: Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation
    Article Snippet: .. The sample was then analysed by liquid chromatography–tandem mass spectometry (LC-MS/MS) using a Velos-LTQ mass spectrometer equipped with a micro-ESI ion source (ThermoFisher, San Jose, CA, USA). ..

    Chromatography:

    Article Title: Short-Term Low Temperature Induces Nitro-Oxidative Stress that Deregulates the NADP-Malic Enzyme Function by Tyrosine Nitration in Arabidopsis thaliana
    Article Snippet: .. The resulting sample was subjected to a liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, using a Velos-LTQ mass spectrometer equipped with a micro-ESI ion source (Thermo Fisher, San Jose, CA, USA). ..

    Article Title: Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation
    Article Snippet: .. The sample was then analysed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a Velos-LTQ mass spectrometer equipped with a micro-ESI (electrospray ionization) ion source (ThermoFisher, San Jose, CA, USA). ..

    Article Title: Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation
    Article Snippet: .. The sample was then analysed by liquid chromatography–tandem mass spectometry (LC-MS/MS) using a Velos-LTQ mass spectrometer equipped with a micro-ESI ion source (ThermoFisher, San Jose, CA, USA). ..

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    Thermo Fisher hybrid linear ion trap orbitrap mass spectrometer
    Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ <t>Orbitrap</t> Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).
    Hybrid Linear Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher ltq orbitrap velos mass spectrometer
    A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a <t>LTQ-Orbitrap</t> <t>Velos</t> mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.
    Ltq Orbitrap Velos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 253 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher ltq velos mass spectrometer
    Distinct peptide identification methods produce different QC metrics with similar variation. Five technical replicates of yeast lysate samples were analyzed on a Thermo Fisher <t>LTQ-Velos</t> mass spectrometer. Spectra were identified by a MyriMatch (MM) database
    Ltq Velos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq velos mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    ltq velos mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Journal: Analytical Chemistry

    Article Title: Noncovalently Associated Peptides Observed during Liquid Chromatography-Mass Spectrometry and Their Effect on Cross-Link Analyses

    doi: 10.1021/acs.analchem.8b04037

    Figure Lengend Snippet: Quality control after cross-link identification at a 5% link FDR. (a) Results from cross-linking HSA with sulfo-SDA acquired on an Q Exactive and an LTQ Orbitrap Velos mass spectrometer. The line at 25 Å indicates the distance cutoff for links classified as long distance. The inlet shows the fraction of long-distance links (LDL) in each data set. (b) Score comparison between within-distance linkzs and LDL. LDL showed no significant (ns) deviation from the within-distance links (two-sided Mann–Whitney-U-test at α = 0.05).

    Article Snippet: We observed surprising differences when comparing the identified cross-links using data acquired on two different mass spectrometers: a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap Velos, Thermo Fisher Scientific) and a hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific).

    Techniques: Mass Spectrometry, MANN-WHITNEY

    A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a LTQ-Orbitrap Velos mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.

    Journal: Clinical proteomics

    Article Title: Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients

    doi: 10.1186/1559-0275-11-1

    Figure Lengend Snippet: A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a LTQ-Orbitrap Velos mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.

    Article Snippet: LC-MS/MS analysis Tandem mass spectrometric analysis of the iTRAQ labeled peptides were carried out using LTQ-Orbitrap Velos mass spectrometer (Thermo Scientific, Bremen, Germany) interfaced with Easy nanoLC II (previously Proxeon, Thermo Scientific, Bremen, Germany).

    Techniques: Labeling, Fractionation, Chromatography, Mass Spectrometry, Quantitation Assay, Western Blot

    Identification of linoleic acid in the free fatty acid fraction purified from canine stratum corneum by APCI LC-MS. RSLC Dionex-U3000 coupled to LTQ-Orbitrap Velos Pro, Thermofisher Scientific with an APCI source. a Free fatty acids spectra. b Linoleic acid spectra- standard use

    Journal: Archives of Dermatological Research

    Article Title: Linoleate-enriched diet increases both linoleic acid esterified to omega hydroxy very long chain fatty acids and free ceramides of canine stratum corneum without effect on protein-bound ceramides and skin barrier function

    doi: 10.1007/s00403-018-1845-5

    Figure Lengend Snippet: Identification of linoleic acid in the free fatty acid fraction purified from canine stratum corneum by APCI LC-MS. RSLC Dionex-U3000 coupled to LTQ-Orbitrap Velos Pro, Thermofisher Scientific with an APCI source. a Free fatty acids spectra. b Linoleic acid spectra- standard use

    Article Snippet: A 5 µl sample was injected in a RSLC Dionex-U300 column coupled to a LTQ-Orbitrap Velos Pro mass spectrometer (Thermofisher Scientific, Villebon-sur-Yvette, France) equipped with an atmospheric-pressure chemical ionization (APCI) interface.

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

    Distinct peptide identification methods produce different QC metrics with similar variation. Five technical replicates of yeast lysate samples were analyzed on a Thermo Fisher LTQ-Velos mass spectrometer. Spectra were identified by a MyriMatch (MM) database

    Journal: Analytical chemistry

    Article Title: QuaMeter: Multivendor Performance Metrics for LC-MS/MS Proteomics Instrumentation

    doi: 10.1021/ac300629p

    Figure Lengend Snippet: Distinct peptide identification methods produce different QC metrics with similar variation. Five technical replicates of yeast lysate samples were analyzed on a Thermo Fisher LTQ-Velos mass spectrometer. Spectra were identified by a MyriMatch (MM) database

    Article Snippet: To evaluate this, we employed a yeast lysate LC-MS/MS data set with five technical replicates acquired on a Thermo Fisher LTQ-Velos mass spectrometer.

    Techniques: Mass Spectrometry