vegf165  (Sino Biological)


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  • 94
    Name:
    VEGF165 Protein Human Cynomolgus Recombinant
    Description:
    A DNA sequence encoding the human VEGF 165 isoform P15692 4 Met1 Arg191 was expressed Human and Cynomolgus VEGF165 sequences are identical
    Catalog Number:
    11066-HNAB
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    MVCD1 Protein Human, VEGF Protein Human, VEGF165 Protein Human, VPF Protein Human
    Host:
    Baculovirus-Insect Cells
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    Structured Review

    Sino Biological vegf165
    A DNA sequence encoding the human VEGF 165 isoform P15692 4 Met1 Arg191 was expressed Human and Cynomolgus VEGF165 sequences are identical
    https://www.bioz.com/result/vegf165/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf165 - by Bioz Stars, 2021-05
    94/100 stars

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    Related Articles

    Binding Assay:

    Article Title: A new method for pharmaceutical compounding and storage of anti-VEGF biologics for intravitreal use in silicone oil-free prefilled plastic syringes
    Article Snippet: For all samples, 77 μl was injected by means of an auto-sampler (Spark Holland B.V.). .. VEGF binding enzyme-linked immunosorbent assay (ELISA)96-well EIA/RIA 3590 plates (Corning Costar) were coated with 100 μl 0.5 µg/mL human VEGF165 (Sino Biological) and incubated over night at 4 °C. .. The plates were blocked for 2 hours with 250 μl 4% skimmed milk powder (S) (Sigma-Aldrich) dissolved in PBS (Sigma-Aldrich) (S/PBS), followed by washing four times with PBS containing 0.05% Tween20 (T) (Sigma-Aldrich).

    Enzyme-linked Immunosorbent Assay:

    Article Title: A new method for pharmaceutical compounding and storage of anti-VEGF biologics for intravitreal use in silicone oil-free prefilled plastic syringes
    Article Snippet: For all samples, 77 μl was injected by means of an auto-sampler (Spark Holland B.V.). .. VEGF binding enzyme-linked immunosorbent assay (ELISA)96-well EIA/RIA 3590 plates (Corning Costar) were coated with 100 μl 0.5 µg/mL human VEGF165 (Sino Biological) and incubated over night at 4 °C. .. The plates were blocked for 2 hours with 250 μl 4% skimmed milk powder (S) (Sigma-Aldrich) dissolved in PBS (Sigma-Aldrich) (S/PBS), followed by washing four times with PBS containing 0.05% Tween20 (T) (Sigma-Aldrich).

    Incubation:

    Article Title: A new method for pharmaceutical compounding and storage of anti-VEGF biologics for intravitreal use in silicone oil-free prefilled plastic syringes
    Article Snippet: For all samples, 77 μl was injected by means of an auto-sampler (Spark Holland B.V.). .. VEGF binding enzyme-linked immunosorbent assay (ELISA)96-well EIA/RIA 3590 plates (Corning Costar) were coated with 100 μl 0.5 µg/mL human VEGF165 (Sino Biological) and incubated over night at 4 °C. .. The plates were blocked for 2 hours with 250 μl 4% skimmed milk powder (S) (Sigma-Aldrich) dissolved in PBS (Sigma-Aldrich) (S/PBS), followed by washing four times with PBS containing 0.05% Tween20 (T) (Sigma-Aldrich).

    Recombinant:

    Article Title: Enhanced effect of VEGF165 on L-type calcium currents in guinea-pig cardiac ventricular myocytes.
    Article Snippet: .. The mechanisms of vascular endothelial growth factor 165 (VEGF165) on electrical properties of cardiomyocytes have not been fully elucidated. .. The mechanisms of vascular endothelial growth factor 165 (VEGF165) on electrical properties of cardiomyocytes have not been fully elucidated.

    Article Title: Inhibitory effect and molecular mechanism of mesenchymal stem cells on NSCLC cells.
    Article Snippet: Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death.

    Article Title: Barbigerone, an isoflavone, inhibits tumor angiogenesis and human non-small-cell lung cancer xenografts growth through VEGFR2 signaling pathways.
    Article Snippet: We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro. .. We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro. .. We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro.

    Article Title: Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis
    Article Snippet: Phycoerythrin (PE)-conjugated mouse anti-VEGFR2 monoclonal antibody (1:500; FAB357P; clone 12G5) and purified mouse immunoglobulin (Ig)G (1:500; 550874) were purchased from BD Biosciences (Heidelberg, Germany). .. Recombinant human VEGF165 (11066-HNAB-500) was obtained from Sino Biological, Inc. (Beijing, China). .. Rabbit anti-β-actin (1:500; ab16039, Abcam) and rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:500; SAB2108266) polyclonal antibodies were obtained from Sigma-Aldrich Chemie GmbH (Deisenhofen, Germany).

    Migration:

    Article Title: Inhibitory effect and molecular mechanism of mesenchymal stem cells on NSCLC cells.
    Article Snippet: Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death.

    Expressing:

    Article Title: Inhibitory effect and molecular mechanism of mesenchymal stem cells on NSCLC cells.
    Article Snippet: Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death.

    Cell Function Assay:

    Article Title: Inhibitory effect and molecular mechanism of mesenchymal stem cells on NSCLC cells.
    Article Snippet: Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death. .. Non-small-cell lung cancer (NSCLC) is still the main threat of cancer-associated death.

    Immunohistochemistry:

    Article Title: Barbigerone, an isoflavone, inhibits tumor angiogenesis and human non-small-cell lung cancer xenografts growth through VEGFR2 signaling pathways.
    Article Snippet: We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro. .. We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro. .. We previously reported that barbigerone (BA), an isoflavone isolated from Suberect Spatholobus, exhibited inhibitory effects on proliferation of many cancer cell lines in vitro.

    Generated:

    Article Title: Immunogenicity and efficacy of a rationally designed vaccine against vascular endothelial growth factor in mouse solid tumor models.
    Article Snippet: .. Vascular endothelial growth factor (VEGF) plays an important role in the progression of various cancers. ..

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  • 92
    Sino Biological elisa vegf 165 antigen protein
    Transfer of the VEGF MAR-HAC vector to CHO K1 cells promotes antibody production. ( A ) Two-color FISH analysis of the VEGF MAR-HAC vector in G418-resistant CHO K1 clones was performed with digoxigenin-labeled hCot-1 DNA (red) and biotin-labeled anti-VEGF antibody gene (green). The arrow indicates the HAC, and the inset shows enlarged images of the HAC (anti-VEGF antibody probe-specific green signals are indicated by arrows). The arrowheads indicate the three large metacentric chromosomes that identify CHO K1 cells. The left panel shows CHO K1R VEGF MAR-HAC cl.1. The right panel shows CHO K1R VEGF MAR-HAC cl.2. ( B ) Analysis of anti-VEGF antibody production with <t>ELISA</t> from CHO K1R VEGF MAR-HAC clones and CHO DG44 VEGF MAR-HAC clones from day 2 to day 8. Bars correspond to the means ± SD of three independent experiments. ( C ) Culture supernatants were analyzed by SDS-PAGE. The protein bands were stained by silver staining. MW, molecular weight markers; line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody (bevacizumab; 0.1 µg). Hc means heavy chain. Lc means light chain. ( D ) Western blotting of the 19.1 kDa <t>VEGF</t> 165 protein with the purified antibody from the CHO cell clones. Line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody.
    Elisa Vegf 165 Antigen Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa vegf 165 antigen protein/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa vegf 165 antigen protein - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    94
    Sino Biological vegf165
    Transfer of the VEGF MAR-HAC vector to CHO K1 cells promotes antibody production. ( A ) Two-color FISH analysis of the VEGF MAR-HAC vector in G418-resistant CHO K1 clones was performed with digoxigenin-labeled hCot-1 DNA (red) and biotin-labeled anti-VEGF antibody gene (green). The arrow indicates the HAC, and the inset shows enlarged images of the HAC (anti-VEGF antibody probe-specific green signals are indicated by arrows). The arrowheads indicate the three large metacentric chromosomes that identify CHO K1 cells. The left panel shows CHO K1R VEGF MAR-HAC cl.1. The right panel shows CHO K1R VEGF MAR-HAC cl.2. ( B ) Analysis of anti-VEGF antibody production with <t>ELISA</t> from CHO K1R VEGF MAR-HAC clones and CHO DG44 VEGF MAR-HAC clones from day 2 to day 8. Bars correspond to the means ± SD of three independent experiments. ( C ) Culture supernatants were analyzed by SDS-PAGE. The protein bands were stained by silver staining. MW, molecular weight markers; line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody (bevacizumab; 0.1 µg). Hc means heavy chain. Lc means light chain. ( D ) Western blotting of the 19.1 kDa <t>VEGF</t> 165 protein with the purified antibody from the CHO cell clones. Line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody.
    Vegf165, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf165/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf165 - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    N/A
    This antibody was obtained from a rabbit immunized with purified recombinant Human VEGF165 VEGFA rh VEGF165 VEGFA Catalog 11066 HNAB P15692 4 Met 1 Arg 191
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    N/A
    Produced in rabbits immunized with purified recombinant Human VEGF165 VEGFA rh VEGF165 VEGFA Catalog 11066 HNAH P15692 4 Met1 Arg191 VEGF165 VEGFA specific IgG was purified by Human VEGF165 VEGFA
      Buy from Supplier

    Image Search Results


    Transfer of the VEGF MAR-HAC vector to CHO K1 cells promotes antibody production. ( A ) Two-color FISH analysis of the VEGF MAR-HAC vector in G418-resistant CHO K1 clones was performed with digoxigenin-labeled hCot-1 DNA (red) and biotin-labeled anti-VEGF antibody gene (green). The arrow indicates the HAC, and the inset shows enlarged images of the HAC (anti-VEGF antibody probe-specific green signals are indicated by arrows). The arrowheads indicate the three large metacentric chromosomes that identify CHO K1 cells. The left panel shows CHO K1R VEGF MAR-HAC cl.1. The right panel shows CHO K1R VEGF MAR-HAC cl.2. ( B ) Analysis of anti-VEGF antibody production with ELISA from CHO K1R VEGF MAR-HAC clones and CHO DG44 VEGF MAR-HAC clones from day 2 to day 8. Bars correspond to the means ± SD of three independent experiments. ( C ) Culture supernatants were analyzed by SDS-PAGE. The protein bands were stained by silver staining. MW, molecular weight markers; line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody (bevacizumab; 0.1 µg). Hc means heavy chain. Lc means light chain. ( D ) Western blotting of the 19.1 kDa VEGF 165 protein with the purified antibody from the CHO cell clones. Line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody.

    Journal: Scientific Reports

    Article Title: An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells

    doi: 10.1038/s41598-019-53116-2

    Figure Lengend Snippet: Transfer of the VEGF MAR-HAC vector to CHO K1 cells promotes antibody production. ( A ) Two-color FISH analysis of the VEGF MAR-HAC vector in G418-resistant CHO K1 clones was performed with digoxigenin-labeled hCot-1 DNA (red) and biotin-labeled anti-VEGF antibody gene (green). The arrow indicates the HAC, and the inset shows enlarged images of the HAC (anti-VEGF antibody probe-specific green signals are indicated by arrows). The arrowheads indicate the three large metacentric chromosomes that identify CHO K1 cells. The left panel shows CHO K1R VEGF MAR-HAC cl.1. The right panel shows CHO K1R VEGF MAR-HAC cl.2. ( B ) Analysis of anti-VEGF antibody production with ELISA from CHO K1R VEGF MAR-HAC clones and CHO DG44 VEGF MAR-HAC clones from day 2 to day 8. Bars correspond to the means ± SD of three independent experiments. ( C ) Culture supernatants were analyzed by SDS-PAGE. The protein bands were stained by silver staining. MW, molecular weight markers; line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody (bevacizumab; 0.1 µg). Hc means heavy chain. Lc means light chain. ( D ) Western blotting of the 19.1 kDa VEGF 165 protein with the purified antibody from the CHO cell clones. Line 1, antibody purified from CHO DG44 VEGF MAR-HAC cl.2; line 2, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 3, antibody purified from CHO K1R VEGF MAR-HAC cl.2; line 4, commercial human anti-VEGF antibody.

    Article Snippet: ELISA VEGF 165 antigen protein (11066-HNAH, Sino Biological, Beijing, China) was diluted with PBS to 50 ng/well, added to a 96-well plate, and incubated for 1 hr at room temperature to immobilize the antigen.

    Techniques: HAC Assay, Plasmid Preparation, Fluorescence In Situ Hybridization, Clone Assay, Labeling, Enzyme-linked Immunosorbent Assay, SDS Page, Staining, Silver Staining, Molecular Weight, Purification, Western Blot