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Millipore vegf mrna
<t>VEGF</t> siRNA-generating pDNA down regulates VEGF <t>mRNA</t> levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p
Vegf Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis"

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

Journal: Reproductive Biology and Endocrinology : RB & E

doi: 10.1186/1477-7827-6-64

VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p
Figure Legend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p

Techniques Used: In Vivo

VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p
Figure Legend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p

Techniques Used: In Vitro, Transfection, Cell Culture, In Vivo

2) Product Images from "CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells"

Article Title: CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells

Journal: Oncology Letters

doi: 10.3892/ol.2017.7369

mRNA and protein expression levels of HIF-1α, CXCR4 and VEGF following treatment with CoCl 2 . (A) Reverse transcription-quantitative polymerase chain reaction analysis of HIF-1α, CXCR4 and VEGF mRNA levels following treatment with CoCl 2 (n=3). (B) Changes in HIF-1α, CXCR4 and VEGF protein expression following treatment with 150 µM CoCl 2 (n=3). *P
Figure Legend Snippet: mRNA and protein expression levels of HIF-1α, CXCR4 and VEGF following treatment with CoCl 2 . (A) Reverse transcription-quantitative polymerase chain reaction analysis of HIF-1α, CXCR4 and VEGF mRNA levels following treatment with CoCl 2 (n=3). (B) Changes in HIF-1α, CXCR4 and VEGF protein expression following treatment with 150 µM CoCl 2 (n=3). *P

Techniques Used: Expressing, Real-time Polymerase Chain Reaction

Related Articles

Isolation:

Article Title: CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells
Article Snippet: .. RT-qPCR was performed to quantitatively estimate the changes in the expression of HIF-1α, CXCR4 and VEGF mRNA in MCF-7 cells treated with the aforementioned CoCl2 concentrations for 24 h. Total RNA was isolated from cells using an RNeasy kit (Sigma-Aldrich; Merck KGaA). .. The RNA was reverse-transcribed into cDNA using the PrimeScript® First Strand cDNA Synthesis kit (Takara Bio, Inc., Otsu, Japan) at 42°C for 15 min, then at 85°C for 5 min.

Quantitative RT-PCR:

Article Title: CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells
Article Snippet: .. RT-qPCR was performed to quantitatively estimate the changes in the expression of HIF-1α, CXCR4 and VEGF mRNA in MCF-7 cells treated with the aforementioned CoCl2 concentrations for 24 h. Total RNA was isolated from cells using an RNeasy kit (Sigma-Aldrich; Merck KGaA). .. The RNA was reverse-transcribed into cDNA using the PrimeScript® First Strand cDNA Synthesis kit (Takara Bio, Inc., Otsu, Japan) at 42°C for 15 min, then at 85°C for 5 min.

Mouse Assay:

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
Article Snippet: .. Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below. .. The animals were euthanized by a lethal injection, as described earlier.

Real-time Polymerase Chain Reaction:

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
Article Snippet: .. Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below. .. The animals were euthanized by a lethal injection, as described earlier.

Microarray:

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
Article Snippet: .. Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below. .. The animals were euthanized by a lethal injection, as described earlier.

Expressing:

Article Title: CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells
Article Snippet: .. RT-qPCR was performed to quantitatively estimate the changes in the expression of HIF-1α, CXCR4 and VEGF mRNA in MCF-7 cells treated with the aforementioned CoCl2 concentrations for 24 h. Total RNA was isolated from cells using an RNeasy kit (Sigma-Aldrich; Merck KGaA). .. The RNA was reverse-transcribed into cDNA using the PrimeScript® First Strand cDNA Synthesis kit (Takara Bio, Inc., Otsu, Japan) at 42°C for 15 min, then at 85°C for 5 min.

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
Article Snippet: .. Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below. .. The animals were euthanized by a lethal injection, as described earlier.

Recombinant:

Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis
Article Snippet: .. Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below. .. The animals were euthanized by a lethal injection, as described earlier.

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  • 99
    Millipore vegf a mrna expression
    siRNA was used to deplete endogenous LSD1 in cultured hormone dependent (LnCaP: panels A, D, G, J, M, P) and hormone independent cells (LnCaP:C4‐2, panels B, E, H, K, N, Q) and PC3, panels C, F, J, M, P, S. siRNA effectively reduced LSD1 protein (A–C) and <t>mRNA</t> (D–F) as compared to the cells transfected with an siRNA targeting luciferase. Reduced LSD1 levels decreased <t>VEGF‐A</t> mRNA (H–J). Functional depletion of LSD1 reduced PSA/KLK3 mRNA expression in hormone dependent LnCaP cells (K), but had no effect in hormone independent prostate cancer cells (L, M). Functional depletion of LSD1 reduced Tmprss2 mRNA expression in LnCaP and PC3 cells (N, P). Secreted VEGF‐A protein levels were quantified using an ELISA and compared to cultured prostate cancer cells transfected with luciferase control siRNA. Knockdown of LSD1 decreased secreted VEGF‐A levels in hormone dependent LnCaP (Q) and hormone independent LnCaP:C4‐2 and PC3 (R, S) cells P values: * = p
    Vegf A Mrna Expression, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf a mrna expression/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf a mrna expression - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    Millipore vegf mrna
    <t>VEGF</t> siRNA-generating pDNA down regulates VEGF <t>mRNA</t> levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p
    Vegf Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf mrna/product/Millipore
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    vegf mrna - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    siRNA was used to deplete endogenous LSD1 in cultured hormone dependent (LnCaP: panels A, D, G, J, M, P) and hormone independent cells (LnCaP:C4‐2, panels B, E, H, K, N, Q) and PC3, panels C, F, J, M, P, S. siRNA effectively reduced LSD1 protein (A–C) and mRNA (D–F) as compared to the cells transfected with an siRNA targeting luciferase. Reduced LSD1 levels decreased VEGF‐A mRNA (H–J). Functional depletion of LSD1 reduced PSA/KLK3 mRNA expression in hormone dependent LnCaP cells (K), but had no effect in hormone independent prostate cancer cells (L, M). Functional depletion of LSD1 reduced Tmprss2 mRNA expression in LnCaP and PC3 cells (N, P). Secreted VEGF‐A protein levels were quantified using an ELISA and compared to cultured prostate cancer cells transfected with luciferase control siRNA. Knockdown of LSD1 decreased secreted VEGF‐A levels in hormone dependent LnCaP (Q) and hormone independent LnCaP:C4‐2 and PC3 (R, S) cells P values: * = p

    Journal: Molecular Oncology

    Article Title: The lysine specific demethylase-1 (LSD1/KDM1A) regulates VEGF-A expression in prostate cancer

    doi: 10.1016/j.molonc.2013.01.003

    Figure Lengend Snippet: siRNA was used to deplete endogenous LSD1 in cultured hormone dependent (LnCaP: panels A, D, G, J, M, P) and hormone independent cells (LnCaP:C4‐2, panels B, E, H, K, N, Q) and PC3, panels C, F, J, M, P, S. siRNA effectively reduced LSD1 protein (A–C) and mRNA (D–F) as compared to the cells transfected with an siRNA targeting luciferase. Reduced LSD1 levels decreased VEGF‐A mRNA (H–J). Functional depletion of LSD1 reduced PSA/KLK3 mRNA expression in hormone dependent LnCaP cells (K), but had no effect in hormone independent prostate cancer cells (L, M). Functional depletion of LSD1 reduced Tmprss2 mRNA expression in LnCaP and PC3 cells (N, P). Secreted VEGF‐A protein levels were quantified using an ELISA and compared to cultured prostate cancer cells transfected with luciferase control siRNA. Knockdown of LSD1 decreased secreted VEGF‐A levels in hormone dependent LnCaP (Q) and hormone independent LnCaP:C4‐2 and PC3 (R, S) cells P values: * = p

    Article Snippet: We also examined the effects of 5, 10 and 50 μM S2101 (Calbiochem, EMD Millipore) on VEGF‐A mRNA expression in PC3 cells treated for 24 and 72 h. For the S2101 experiment a minimum of five biological replicates conducted over two independent experiments were examined in triplicate qPCRs.

    Techniques: Cell Culture, Transfection, Luciferase, Functional Assay, Expressing, Enzyme-linked Immunosorbent Assay

    VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

    doi: 10.1186/1477-7827-6-64

    Figure Lengend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p

    Article Snippet: Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below.

    Techniques: In Vivo

    VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

    doi: 10.1186/1477-7827-6-64

    Figure Lengend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p

    Article Snippet: Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below.

    Techniques: In Vitro, Transfection, Cell Culture, In Vivo