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    Beijing Solarbio Science rabbit vascular endothelial growth factor vegf elisa kit
    Rabbit Vascular Endothelial Growth Factor Vegf Elisa Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human vegf a elisa kit  (Millipore)

     
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    human vegf elisa kit  (Thermo Fisher)


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    Thermo Fisher human vegf elisa kit
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    R&D Systems vegf elisa kit
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    human vegf a elisa kit  (Millipore)

     
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    Millipore human vegf a elisa kit
    FGFR, VEGFR, and KIT signaling pathways in IM-naive (GIST T-1) and IM-resistant (GIST T-1R, GIST 430) cells. ( A ) Expression of FGF/FGFR signaling proteins in GIST cells; ( B ) expression of the VEGF/FGFR signaling proteins in GIST cells; ( C ) changes in the relative expression level of <t>VEGF-A</t> and VEGFR1, 2, and 3 in GIST T-1 vs. T-1R and GIST 430 cells, as determined by quantitative RT-PCR. For internal control, the amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used; data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. ( D ) Expression of the KIT signaling proteins in GIST cells. Actin stain was used as a loading control for all experiments shown in ( A , B , D ). ( E ) Concentration of VEGF-A (pg/mL) in supernatants of IM-naive (GIST T-1) and resistant (GIST T-1R and 430) cells measured by <t>ELISA,</t> as described in . Data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. The original Western blot figures can be found in .
    Human Vegf A Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fibroblast Growth Factor 2 (FGF2) Activates Vascular Endothelial Growth Factor (VEGF) Signaling in Gastrointestinal Stromal Tumors (GIST): An Autocrine Mechanism Contributing to Imatinib Mesylate (IM) Resistance"

    Article Title: Fibroblast Growth Factor 2 (FGF2) Activates Vascular Endothelial Growth Factor (VEGF) Signaling in Gastrointestinal Stromal Tumors (GIST): An Autocrine Mechanism Contributing to Imatinib Mesylate (IM) Resistance

    Journal: Cancers

    doi: 10.3390/cancers16173103

    FGFR, VEGFR, and KIT signaling pathways in IM-naive (GIST T-1) and IM-resistant (GIST T-1R, GIST 430) cells. ( A ) Expression of FGF/FGFR signaling proteins in GIST cells; ( B ) expression of the VEGF/FGFR signaling proteins in GIST cells; ( C ) changes in the relative expression level of VEGF-A and VEGFR1, 2, and 3 in GIST T-1 vs. T-1R and GIST 430 cells, as determined by quantitative RT-PCR. For internal control, the amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used; data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. ( D ) Expression of the KIT signaling proteins in GIST cells. Actin stain was used as a loading control for all experiments shown in ( A , B , D ). ( E ) Concentration of VEGF-A (pg/mL) in supernatants of IM-naive (GIST T-1) and resistant (GIST T-1R and 430) cells measured by ELISA, as described in . Data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. The original Western blot figures can be found in .
    Figure Legend Snippet: FGFR, VEGFR, and KIT signaling pathways in IM-naive (GIST T-1) and IM-resistant (GIST T-1R, GIST 430) cells. ( A ) Expression of FGF/FGFR signaling proteins in GIST cells; ( B ) expression of the VEGF/FGFR signaling proteins in GIST cells; ( C ) changes in the relative expression level of VEGF-A and VEGFR1, 2, and 3 in GIST T-1 vs. T-1R and GIST 430 cells, as determined by quantitative RT-PCR. For internal control, the amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used; data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. ( D ) Expression of the KIT signaling proteins in GIST cells. Actin stain was used as a loading control for all experiments shown in ( A , B , D ). ( E ) Concentration of VEGF-A (pg/mL) in supernatants of IM-naive (GIST T-1) and resistant (GIST T-1R and 430) cells measured by ELISA, as described in . Data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. The original Western blot figures can be found in .

    Techniques Used: Expressing, Quantitative RT-PCR, Control, Amplification, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot

    ( A , B ) The exogenous FGF2 activates VEGFR signaling in GIST T-1 cells via up-regulation of VEGF-A. ( A ) FGF-2 stimulates, whereas anti-FGF2 neutralizing Abs abrogates VEGFR signaling GIST T-1 cells. Cells were treated with FGF2 (100 ng/mL), IM (0.02 µM) alone or in the presence of anti-FGF2 neutralizing Abs (20 µg/mL) for 72 h. Expression of VEGF-A, total and phosphorylated forms of VEGFR, KIT, MAPK, AKT, and STAT1 was assessed by immunoblot analysis. Actin stain was used as a loading control for each sample. ( B ) Concentration of VEGF-A (pg/mL, measured by ELISA) in supernatants of IM-naive (GIST T-1) cells treated with DMSO (control), FGF-2 (100 ng/mL), IM (0.02 µM) alone or in the presence of anti-FGF-2 Abs (20 µg/mL) for 72 h. Data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. ( C ) Changes in the relative expression level of mRNA VEGF-A and VEGFR1, 2, and 3 in GIST T-1 after treatment with FGF-2 (100 ng/mL), as determined by quantitative RT-PCR. The amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used for internal control. Data are presented as median ± SD. Significant differences with p < 0.05 (*) from n ≥ 3 using unpaired Student’s t -test. The original Western blot figures can be found in .
    Figure Legend Snippet: ( A , B ) The exogenous FGF2 activates VEGFR signaling in GIST T-1 cells via up-regulation of VEGF-A. ( A ) FGF-2 stimulates, whereas anti-FGF2 neutralizing Abs abrogates VEGFR signaling GIST T-1 cells. Cells were treated with FGF2 (100 ng/mL), IM (0.02 µM) alone or in the presence of anti-FGF2 neutralizing Abs (20 µg/mL) for 72 h. Expression of VEGF-A, total and phosphorylated forms of VEGFR, KIT, MAPK, AKT, and STAT1 was assessed by immunoblot analysis. Actin stain was used as a loading control for each sample. ( B ) Concentration of VEGF-A (pg/mL, measured by ELISA) in supernatants of IM-naive (GIST T-1) cells treated with DMSO (control), FGF-2 (100 ng/mL), IM (0.02 µM) alone or in the presence of anti-FGF-2 Abs (20 µg/mL) for 72 h. Data are presented as median ± SD. Significant differences with p < 0.05 (*), p < 0.01 (**) from n ≥ 3 using unpaired Student’s t -test. ( C ) Changes in the relative expression level of mRNA VEGF-A and VEGFR1, 2, and 3 in GIST T-1 after treatment with FGF-2 (100 ng/mL), as determined by quantitative RT-PCR. The amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used for internal control. Data are presented as median ± SD. Significant differences with p < 0.05 (*) from n ≥ 3 using unpaired Student’s t -test. The original Western blot figures can be found in .

    Techniques Used: Expressing, Western Blot, Staining, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Amplification

    Inhibition of KIT signaling activates FGFR and VEGFR signaling via overproduction of FGF-2 and VEGF-A in IM-resistant GIST-T1 cells.
    Figure Legend Snippet: Inhibition of KIT signaling activates FGFR and VEGFR signaling via overproduction of FGF-2 and VEGF-A in IM-resistant GIST-T1 cells.

    Techniques Used: Inhibition

    human vegf elisa kit  (R&D Systems Hematology)

     
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    R&D Systems Hematology human vegf elisa kit
    Human Vegf Elisa Kit, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vegf elisa kit  (R&D Systems)


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    R&D Systems vegf elisa kit
    Vegf Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vegf mouse vegf elisa kit beyotime  (Beyotime)

     
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    Beyotime vegf mouse vegf elisa kit beyotime
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    vegf elisa kit  (Enzo Biochem)

     
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    Enzo Biochem vegf elisa kit
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    vegf elisa kit  (R&D Systems)


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    R&D Systems vegf elisa kit
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