vector prsetb  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Thermo Fisher vector prsetb
    Vector Prsetb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector prsetb/product/Thermo Fisher
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    vector prsetb - by Bioz Stars, 2020-08
    89/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine)
    Article Snippet: .. Both dsRed (CLONTECH) and Citrine DNAs were cloned into the vector pRSETB (Invitrogen). .. Proteins were produced in JM109 (DE3) Escherichia coli , purified by nickel chelate chromatography on NiNTA-agarose, dialyzed against 10 mM Tris⋅HCl, pH 7.5.

    Article Title: Mechanisms Governing Expression of the v-FLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: .. For the generation of bicistronic ORF 72/luciferase (LUC) reporter constructs, a Bam HI/ Eco RI restriction fragment containing ORF 71 was removed from the construct pCMV 72/71 and cloned into the vector pRSETB (Invitrogen), ORF 71 was excised with the restriction enzymes Nco I and Bgl II and replaced by the coding region of the Photinus luciferase amplified from the vector pGL3-basic (Promega), and the cassette was reinserted into the parental construct pCMV 72/71 using the Bam HI and Eco RI restriction sites to yield construct pCMV 72/LUC. .. Additional reporters were generated based on these constructs: (i) the region upstream of a Hin dIII site overlapping the ORF 72 stop codon was excised to generate a monocistronic luciferase construct (pCMV LUC) which served as a positive control; overlapping PCR with primers containing the desired mutations was used (ii) to eliminate the ORF 72 start codon (GCTCGCCACTCTAT ATG GCA → GCTCGCCACTCTAT CGAT CA; the ORF 72 start codon is shown in bold and mutated nucleotides are underlined) or (iii) to optimize the nucleotides surrounding the ORF 72 start codon with regard to the efficiency of translational initiation (GCTCGCCACTCTAT ATG GCA → GCTCG G C CGC C ACC ATG GCA) ( ); (iv) the 82-bp noncoding segment between the Hin dIII and Nco I sites overlapping the ORF 72 stop codon or the ORF 71 start codon, respectively, was replaced by an unrelated nucleotide sequence of 83 bp consisting of a Sca I- Nco I polylinker fragment of the vector pGL3 basic (Invitrogen); (v) with the exception of the 16 nucleotides (nt) immediately upstream of the ORF 72 start codon, the complete 5′ region, including a small uORF, was removed.

    Luciferase:

    Article Title: Mechanisms Governing Expression of the v-FLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: .. For the generation of bicistronic ORF 72/luciferase (LUC) reporter constructs, a Bam HI/ Eco RI restriction fragment containing ORF 71 was removed from the construct pCMV 72/71 and cloned into the vector pRSETB (Invitrogen), ORF 71 was excised with the restriction enzymes Nco I and Bgl II and replaced by the coding region of the Photinus luciferase amplified from the vector pGL3-basic (Promega), and the cassette was reinserted into the parental construct pCMV 72/71 using the Bam HI and Eco RI restriction sites to yield construct pCMV 72/LUC. .. Additional reporters were generated based on these constructs: (i) the region upstream of a Hin dIII site overlapping the ORF 72 stop codon was excised to generate a monocistronic luciferase construct (pCMV LUC) which served as a positive control; overlapping PCR with primers containing the desired mutations was used (ii) to eliminate the ORF 72 start codon (GCTCGCCACTCTAT ATG GCA → GCTCGCCACTCTAT CGAT CA; the ORF 72 start codon is shown in bold and mutated nucleotides are underlined) or (iii) to optimize the nucleotides surrounding the ORF 72 start codon with regard to the efficiency of translational initiation (GCTCGCCACTCTAT ATG GCA → GCTCG G C CGC C ACC ATG GCA) ( ); (iv) the 82-bp noncoding segment between the Hin dIII and Nco I sites overlapping the ORF 72 stop codon or the ORF 71 start codon, respectively, was replaced by an unrelated nucleotide sequence of 83 bp consisting of a Sca I- Nco I polylinker fragment of the vector pGL3 basic (Invitrogen); (v) with the exception of the 16 nucleotides (nt) immediately upstream of the ORF 72 start codon, the complete 5′ region, including a small uORF, was removed.

    Subcloning:

    Article Title: Physical and functional interactions between members of the tumour suppressor p53 and the Sp families of transcription factors: importance for the regulation of genes involved in cell-cycle arrest and apoptosis
    Article Snippet: .. The bacterial expression vector for wild-type p53 fused with the His6 tag was constructed by subcloning the full-length human p53 cDNA into the EcoRI and HindIII sites of vector pRSETB (Invitrogen). ..

    Construct:

    Article Title: Physical and functional interactions between members of the tumour suppressor p53 and the Sp families of transcription factors: importance for the regulation of genes involved in cell-cycle arrest and apoptosis
    Article Snippet: .. The bacterial expression vector for wild-type p53 fused with the His6 tag was constructed by subcloning the full-length human p53 cDNA into the EcoRI and HindIII sites of vector pRSETB (Invitrogen). ..

    Article Title: Mechanisms Governing Expression of the v-FLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: .. For the generation of bicistronic ORF 72/luciferase (LUC) reporter constructs, a Bam HI/ Eco RI restriction fragment containing ORF 71 was removed from the construct pCMV 72/71 and cloned into the vector pRSETB (Invitrogen), ORF 71 was excised with the restriction enzymes Nco I and Bgl II and replaced by the coding region of the Photinus luciferase amplified from the vector pGL3-basic (Promega), and the cassette was reinserted into the parental construct pCMV 72/71 using the Bam HI and Eco RI restriction sites to yield construct pCMV 72/LUC. .. Additional reporters were generated based on these constructs: (i) the region upstream of a Hin dIII site overlapping the ORF 72 stop codon was excised to generate a monocistronic luciferase construct (pCMV LUC) which served as a positive control; overlapping PCR with primers containing the desired mutations was used (ii) to eliminate the ORF 72 start codon (GCTCGCCACTCTAT ATG GCA → GCTCGCCACTCTAT CGAT CA; the ORF 72 start codon is shown in bold and mutated nucleotides are underlined) or (iii) to optimize the nucleotides surrounding the ORF 72 start codon with regard to the efficiency of translational initiation (GCTCGCCACTCTAT ATG GCA → GCTCG G C CGC C ACC ATG GCA) ( ); (iv) the 82-bp noncoding segment between the Hin dIII and Nco I sites overlapping the ORF 72 stop codon or the ORF 71 start codon, respectively, was replaced by an unrelated nucleotide sequence of 83 bp consisting of a Sca I- Nco I polylinker fragment of the vector pGL3 basic (Invitrogen); (v) with the exception of the 16 nucleotides (nt) immediately upstream of the ORF 72 start codon, the complete 5′ region, including a small uORF, was removed.

    Purification:

    Article Title: Dissociation from the Oligomeric State Is the Rate-limiting Step in Fibril Formation by ?-Casein *
    Article Snippet: .. The vector pRSETB (Invitrogen), containing the human α-synuclein A53T gene, was a kind gift from Dr. Roberto Cappai (University of Melbourne, Australia), and the α-synuclein A53T protein was expressed and purified as described previously ( ). .. Thioflavin T (ThT), 1,4-dithiothreitol, proteinase K (molecular biology grade), and β-mercaptoethanol were obtained from Sigma.

    Amplification:

    Article Title: Mechanisms Governing Expression of the v-FLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: .. For the generation of bicistronic ORF 72/luciferase (LUC) reporter constructs, a Bam HI/ Eco RI restriction fragment containing ORF 71 was removed from the construct pCMV 72/71 and cloned into the vector pRSETB (Invitrogen), ORF 71 was excised with the restriction enzymes Nco I and Bgl II and replaced by the coding region of the Photinus luciferase amplified from the vector pGL3-basic (Promega), and the cassette was reinserted into the parental construct pCMV 72/71 using the Bam HI and Eco RI restriction sites to yield construct pCMV 72/LUC. .. Additional reporters were generated based on these constructs: (i) the region upstream of a Hin dIII site overlapping the ORF 72 stop codon was excised to generate a monocistronic luciferase construct (pCMV LUC) which served as a positive control; overlapping PCR with primers containing the desired mutations was used (ii) to eliminate the ORF 72 start codon (GCTCGCCACTCTAT ATG GCA → GCTCGCCACTCTAT CGAT CA; the ORF 72 start codon is shown in bold and mutated nucleotides are underlined) or (iii) to optimize the nucleotides surrounding the ORF 72 start codon with regard to the efficiency of translational initiation (GCTCGCCACTCTAT ATG GCA → GCTCG G C CGC C ACC ATG GCA) ( ); (iv) the 82-bp noncoding segment between the Hin dIII and Nco I sites overlapping the ORF 72 stop codon or the ORF 71 start codon, respectively, was replaced by an unrelated nucleotide sequence of 83 bp consisting of a Sca I- Nco I polylinker fragment of the vector pGL3 basic (Invitrogen); (v) with the exception of the 16 nucleotides (nt) immediately upstream of the ORF 72 start codon, the complete 5′ region, including a small uORF, was removed.

    Expressing:

    Article Title: Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity
    Article Snippet: .. The plasmid encoding the MT1-MMP biosensor with an N-terminal 6x -His tag, which was subcloned into vector pRSETb (Invitrogen) using Bgl II/ Hind III sites for bacterial expression, is transformed into BL21(DE3) competent E. Coli . ..

    Article Title: Physical and functional interactions between members of the tumour suppressor p53 and the Sp families of transcription factors: importance for the regulation of genes involved in cell-cycle arrest and apoptosis
    Article Snippet: .. The bacterial expression vector for wild-type p53 fused with the His6 tag was constructed by subcloning the full-length human p53 cDNA into the EcoRI and HindIII sites of vector pRSETB (Invitrogen). ..

    Polymerase Chain Reaction:

    Article Title: Mucosal immunization of mice with recombinant OMP P2 induces antibodies that bind to surface epitopes of multiple strains of nontypeable Haemophilus influenzae
    Article Snippet: .. The PCR products were digested with Bam HI (New England Biolabs, Ipswich, MA) and ligated into the vector pRSETB (Invitrogen), which places a 6X-Histidine tag on the N terminus of the recombinant protein. .. Ligated plasmid was transformed into chemically competent TOP 10 cells and positive clones were selected by plating onto Luria–Bertani plates containing carbenicillin.

    Article Title: Molecular Cloning of the Pasteurella haemolytica pomA Gene and Identification of Bovine Antibodies against PomA Surface Domains
    Article Snippet: .. The region of pomA encoding the mature form of the protein was amplified by PCR and cloned into the vector pRSETB (Invitrogen), downstream of and in-frame with sequences encoding an N-terminal fusion peptide with a metal binding domain. .. DNA sequencing of fusion protein coding regions was performed to verify that no errors occurred during amplification. rPomA was purified by immobilized metal affinity chromatography according to the instructions of the manufacturer (Invitrogen).

    Recombinant:

    Article Title: Mucosal immunization of mice with recombinant OMP P2 induces antibodies that bind to surface epitopes of multiple strains of nontypeable Haemophilus influenzae
    Article Snippet: .. The PCR products were digested with Bam HI (New England Biolabs, Ipswich, MA) and ligated into the vector pRSETB (Invitrogen), which places a 6X-Histidine tag on the N terminus of the recombinant protein. .. Ligated plasmid was transformed into chemically competent TOP 10 cells and positive clones were selected by plating onto Luria–Bertani plates containing carbenicillin.

    Transformation Assay:

    Article Title: Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity
    Article Snippet: .. The plasmid encoding the MT1-MMP biosensor with an N-terminal 6x -His tag, which was subcloned into vector pRSETb (Invitrogen) using Bgl II/ Hind III sites for bacterial expression, is transformed into BL21(DE3) competent E. Coli . ..

    Binding Assay:

    Article Title: Molecular Cloning of the Pasteurella haemolytica pomA Gene and Identification of Bovine Antibodies against PomA Surface Domains
    Article Snippet: .. The region of pomA encoding the mature form of the protein was amplified by PCR and cloned into the vector pRSETB (Invitrogen), downstream of and in-frame with sequences encoding an N-terminal fusion peptide with a metal binding domain. .. DNA sequencing of fusion protein coding regions was performed to verify that no errors occurred during amplification. rPomA was purified by immobilized metal affinity chromatography according to the instructions of the manufacturer (Invitrogen).

    Plasmid Preparation:

    Article Title: Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine)
    Article Snippet: .. Both dsRed (CLONTECH) and Citrine DNAs were cloned into the vector pRSETB (Invitrogen). .. Proteins were produced in JM109 (DE3) Escherichia coli , purified by nickel chelate chromatography on NiNTA-agarose, dialyzed against 10 mM Tris⋅HCl, pH 7.5.

    Article Title: Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity
    Article Snippet: .. The plasmid encoding the MT1-MMP biosensor with an N-terminal 6x -His tag, which was subcloned into vector pRSETb (Invitrogen) using Bgl II/ Hind III sites for bacterial expression, is transformed into BL21(DE3) competent E. Coli . ..

    Article Title: Physical and functional interactions between members of the tumour suppressor p53 and the Sp families of transcription factors: importance for the regulation of genes involved in cell-cycle arrest and apoptosis
    Article Snippet: .. The bacterial expression vector for wild-type p53 fused with the His6 tag was constructed by subcloning the full-length human p53 cDNA into the EcoRI and HindIII sites of vector pRSETB (Invitrogen). ..

    Article Title: Mechanisms Governing Expression of the v-FLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: .. For the generation of bicistronic ORF 72/luciferase (LUC) reporter constructs, a Bam HI/ Eco RI restriction fragment containing ORF 71 was removed from the construct pCMV 72/71 and cloned into the vector pRSETB (Invitrogen), ORF 71 was excised with the restriction enzymes Nco I and Bgl II and replaced by the coding region of the Photinus luciferase amplified from the vector pGL3-basic (Promega), and the cassette was reinserted into the parental construct pCMV 72/71 using the Bam HI and Eco RI restriction sites to yield construct pCMV 72/LUC. .. Additional reporters were generated based on these constructs: (i) the region upstream of a Hin dIII site overlapping the ORF 72 stop codon was excised to generate a monocistronic luciferase construct (pCMV LUC) which served as a positive control; overlapping PCR with primers containing the desired mutations was used (ii) to eliminate the ORF 72 start codon (GCTCGCCACTCTAT ATG GCA → GCTCGCCACTCTAT CGAT CA; the ORF 72 start codon is shown in bold and mutated nucleotides are underlined) or (iii) to optimize the nucleotides surrounding the ORF 72 start codon with regard to the efficiency of translational initiation (GCTCGCCACTCTAT ATG GCA → GCTCG G C CGC C ACC ATG GCA) ( ); (iv) the 82-bp noncoding segment between the Hin dIII and Nco I sites overlapping the ORF 72 stop codon or the ORF 71 start codon, respectively, was replaced by an unrelated nucleotide sequence of 83 bp consisting of a Sca I- Nco I polylinker fragment of the vector pGL3 basic (Invitrogen); (v) with the exception of the 16 nucleotides (nt) immediately upstream of the ORF 72 start codon, the complete 5′ region, including a small uORF, was removed.

    Article Title: Mucosal immunization of mice with recombinant OMP P2 induces antibodies that bind to surface epitopes of multiple strains of nontypeable Haemophilus influenzae
    Article Snippet: .. The PCR products were digested with Bam HI (New England Biolabs, Ipswich, MA) and ligated into the vector pRSETB (Invitrogen), which places a 6X-Histidine tag on the N terminus of the recombinant protein. .. Ligated plasmid was transformed into chemically competent TOP 10 cells and positive clones were selected by plating onto Luria–Bertani plates containing carbenicillin.

    Article Title: Dissociation from the Oligomeric State Is the Rate-limiting Step in Fibril Formation by ?-Casein *
    Article Snippet: .. The vector pRSETB (Invitrogen), containing the human α-synuclein A53T gene, was a kind gift from Dr. Roberto Cappai (University of Melbourne, Australia), and the α-synuclein A53T protein was expressed and purified as described previously ( ). .. Thioflavin T (ThT), 1,4-dithiothreitol, proteinase K (molecular biology grade), and β-mercaptoethanol were obtained from Sigma.

    Article Title: The Bradyrhizobium japonicum nolA Gene Encodes Three Functionally Distinct Proteins
    Article Snippet: .. To generate large amounts of NolA for antibody production, a polyhistidine tag system was used to express NolA as a fusion protein from the T7 promoter of the vector pRSETB (Invitrogen, San Diego, Calif.). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher prset c bacterial expression vector
    Prset C Bacterial Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prset c bacterial expression vector/product/Thermo Fisher
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    prset c bacterial expression vector - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher plasmid prset emgfp
    <t>pRSET-EmGFP</t> plasmid map used to transform Escherichia coli. The mouse interleukin (IL-22) construct was inserted into the vector between the BamHI and NcoI restriction sites (blue arrow) forming pRSET-IL-22-EmGFP.
    Plasmid Prset Emgfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid prset emgfp/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    plasmid prset emgfp - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher prseta hspa1 plasmid
    Shake flask cultivations of E. coli BL21(DE3) with no plasmid (green), BL21(DE3) <t>pRSETA-HspA1</t> (red), BL21(DE3) pET3a-Hsp27 (blue), Rosetta-2 pOPINF- h CNTF-1 (black) cells in 2x YTPG medium. EnBase system cultivations of Rosetta-2 pOPINF- h CNTF-2 are not shown (cell dry weights are listed in Table 1 ). The figure was created with Microsoft Excel version 16.0.4927.1000; https://products.office.com/en/excel ).
    Prseta Hspa1 Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prseta hspa1 plasmid/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prseta hspa1 plasmid - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    pRSET-EmGFP plasmid map used to transform Escherichia coli. The mouse interleukin (IL-22) construct was inserted into the vector between the BamHI and NcoI restriction sites (blue arrow) forming pRSET-IL-22-EmGFP.

    Journal: In Vivo

    Article Title: Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation

    doi: 10.21873/invivo.11743

    Figure Lengend Snippet: pRSET-EmGFP plasmid map used to transform Escherichia coli. The mouse interleukin (IL-22) construct was inserted into the vector between the BamHI and NcoI restriction sites (blue arrow) forming pRSET-IL-22-EmGFP.

    Article Snippet: Plasmid pRSET-EmGFP purchased from Thermo Fisher Scientific was used as E. coli BL21 expression vector.

    Techniques: Plasmid Preparation, Construct

    Shake flask cultivations of E. coli BL21(DE3) with no plasmid (green), BL21(DE3) pRSETA-HspA1 (red), BL21(DE3) pET3a-Hsp27 (blue), Rosetta-2 pOPINF- h CNTF-1 (black) cells in 2x YTPG medium. EnBase system cultivations of Rosetta-2 pOPINF- h CNTF-2 are not shown (cell dry weights are listed in Table 1 ). The figure was created with Microsoft Excel version 16.0.4927.1000; https://products.office.com/en/excel ).

    Journal: Scientific Reports

    Article Title: Assessment of recombinant protein production in E. coli with Time-Gated Surface Enhanced Raman Spectroscopy (TG-SERS)

    doi: 10.1038/s41598-020-59091-3

    Figure Lengend Snippet: Shake flask cultivations of E. coli BL21(DE3) with no plasmid (green), BL21(DE3) pRSETA-HspA1 (red), BL21(DE3) pET3a-Hsp27 (blue), Rosetta-2 pOPINF- h CNTF-1 (black) cells in 2x YTPG medium. EnBase system cultivations of Rosetta-2 pOPINF- h CNTF-2 are not shown (cell dry weights are listed in Table 1 ). The figure was created with Microsoft Excel version 16.0.4927.1000; https://products.office.com/en/excel ).

    Article Snippet: DNA methods The pRSETA-HspA1 plasmid was synthesized and codon optimized for E. coli (ThermoFisher, USA).

    Techniques: Plasmid Preparation