vector pjet1 2 blunt  (Thermo Fisher)


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    Structured Review

    Thermo Fisher vector pjet1 2 blunt
    Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector <t>pJET1.2/blunt,</t> and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.
    Vector Pjet1 2 Blunt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pjet1 2 blunt/product/Thermo Fisher
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    vector pjet1 2 blunt - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "A Closer Look on the Polyhydroxybutyrate- (PHB-) Negative Phenotype of Ralstonia eutropha PHB-4"

    Article Title: A Closer Look on the Polyhydroxybutyrate- (PHB-) Negative Phenotype of Ralstonia eutropha PHB-4

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095907

    Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.
    Figure Legend Snippet: Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.

    Techniques Used: Polymerase Chain Reaction, Plasmid Preparation, Mutagenesis, Functional Assay

    Related Articles

    Clone Assay:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: .. The purified fragments were ligated into a pJET1.2 cloning vector using a CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and transformed into competent E. coli (TOP10 cells). .. Positive clones were selected by colony PCR followed by plasmid isolation with the PrestoTM Mini Plasmid Kit (Geneaid).

    Article Title: Toxin transcripts in Crotalus atrox venom and in silico structures of toxins
    Article Snippet: .. Identification of the most common venom toxin transcripts To identify the most common toxin transcripts of each toxin family from C. atrox crude venom, we adopted the procedure developed in our lab ( ) with some minor modifications: i) we used pJET1.2 (ThermoFisher Scientific, USA) as the cloning vector; and ii) we divided PCR product (20μl) of each clone into two 10μl, one for running agarose gel to obtain the transcript with correct molecular size and the other 10μl for Alu I restriction enzyme digestion of same molecular size to acquire the most common transcript in terms of the enzyme digestion pattern. .. The recombinant plasmid DNAs isolated from the most common clones were sequenced.

    Article Title: CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing Fungus Lichtheimia corymbifera
    Article Snippet: .. The amplified fragment was ligated into the pJet1.2 cloning vector (Thermo Scientific, Waltham, MA, USA), arising the plasmid pLcpyrGcompl ( a), which was used to complement the uracil auxotrophy of the pyrG disrupted mutants. .. The plasmid was transformed into the mutants by PEG-mediated protoplast transformation.

    Article Title: One RNA plays three roles to provide catalytic activity to a group I intron lacking an endogenous internal guide sequence
    Article Snippet: .. The PCR products were cloned into the vector pJET1.2 (Fermentas) and transformed into Escherichia coli . .. Individual colonies were picked as templates for colony PCR reactions employing the primers pJET1.2-F and pJET1.2-R (Fermentas), which generate products of ∼310 bp [= the insert size (∼192 bp) plus about 120 bp].

    Article Title: The transcriptome of Candida albicans mitochondria and the evolution of organellar transcription units in yeasts
    Article Snippet: .. Cycling conditions were 98 °C/3 min, 28 cycles of 98 °C/ 30 s, 55 °C/30 s, 72 °C/30 s, 72 °C/7 min. PCR products were separated on 3 % high resolution agarose gels (Polskie Agarozy), excised, eluted from the gel (Thermo Scientific GeneJET kit) and cloned into pJET1.2 Cloning Vector (Thermo Scientific CloneJET). .. Bacterial clones containing the plasmids with the inserts of proper size were identified by Bgl II digestion.

    Amplification:

    Article Title: CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing Fungus Lichtheimia corymbifera
    Article Snippet: .. The amplified fragment was ligated into the pJet1.2 cloning vector (Thermo Scientific, Waltham, MA, USA), arising the plasmid pLcpyrGcompl ( a), which was used to complement the uracil auxotrophy of the pyrG disrupted mutants. .. The plasmid was transformed into the mutants by PEG-mediated protoplast transformation.

    Agarose Gel Electrophoresis:

    Article Title: Toxin transcripts in Crotalus atrox venom and in silico structures of toxins
    Article Snippet: .. Identification of the most common venom toxin transcripts To identify the most common toxin transcripts of each toxin family from C. atrox crude venom, we adopted the procedure developed in our lab ( ) with some minor modifications: i) we used pJET1.2 (ThermoFisher Scientific, USA) as the cloning vector; and ii) we divided PCR product (20μl) of each clone into two 10μl, one for running agarose gel to obtain the transcript with correct molecular size and the other 10μl for Alu I restriction enzyme digestion of same molecular size to acquire the most common transcript in terms of the enzyme digestion pattern. .. The recombinant plasmid DNAs isolated from the most common clones were sequenced.

    Isolation:

    Article Title: Suggested Role for G4 DNA in Recombinational Switching at the Antigenic Variation Locus of the Lyme Disease Spirochete
    Article Snippet: .. Plasmid DNA was isolated using the Qiagen plasmid miniprep kit and sequenced by the University of Calgary Core DNA Services using the pJET1.2 forward/Custom sequencing primer (CloneJet, Fermentas). ..

    Construct:

    Article Title: Structure and Activity of a Cytosolic Ribosome-Inactivating Protein from Rice
    Article Snippet: .. Construct for Recombinant Protein Production The OsRIP1 coding sequence ligated in the pJET1.2 vector (Thermo Fisher Scientific) was used as a template to prepare the OsRIP1 His6-tagged expression construct. .. DNA sequences encoding a C-terminal Gly3-linker followed by a 6xHis-tag, as well as Gibson assembly sites allowing recombination into the pET21a vector by Gibson assembly, were added to the ORF by two consecutive PCRs.

    Purification:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: .. The purified fragments were ligated into a pJET1.2 cloning vector using a CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and transformed into competent E. coli (TOP10 cells). .. Positive clones were selected by colony PCR followed by plasmid isolation with the PrestoTM Mini Plasmid Kit (Geneaid).

    Sequencing:

    Article Title: Structure and Activity of a Cytosolic Ribosome-Inactivating Protein from Rice
    Article Snippet: .. Construct for Recombinant Protein Production The OsRIP1 coding sequence ligated in the pJET1.2 vector (Thermo Fisher Scientific) was used as a template to prepare the OsRIP1 His6-tagged expression construct. .. DNA sequences encoding a C-terminal Gly3-linker followed by a 6xHis-tag, as well as Gibson assembly sites allowing recombination into the pET21a vector by Gibson assembly, were added to the ORF by two consecutive PCRs.

    Article Title: Suggested Role for G4 DNA in Recombinational Switching at the Antigenic Variation Locus of the Lyme Disease Spirochete
    Article Snippet: .. Plasmid DNA was isolated using the Qiagen plasmid miniprep kit and sequenced by the University of Calgary Core DNA Services using the pJET1.2 forward/Custom sequencing primer (CloneJet, Fermentas). ..

    Expressing:

    Article Title: Structure and Activity of a Cytosolic Ribosome-Inactivating Protein from Rice
    Article Snippet: .. Construct for Recombinant Protein Production The OsRIP1 coding sequence ligated in the pJET1.2 vector (Thermo Fisher Scientific) was used as a template to prepare the OsRIP1 His6-tagged expression construct. .. DNA sequences encoding a C-terminal Gly3-linker followed by a 6xHis-tag, as well as Gibson assembly sites allowing recombination into the pET21a vector by Gibson assembly, were added to the ORF by two consecutive PCRs.

    Polymerase Chain Reaction:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: .. The purified fragments were ligated into a pJET1.2 cloning vector using a CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and transformed into competent E. coli (TOP10 cells). .. Positive clones were selected by colony PCR followed by plasmid isolation with the PrestoTM Mini Plasmid Kit (Geneaid).

    Article Title: Toxin transcripts in Crotalus atrox venom and in silico structures of toxins
    Article Snippet: .. Identification of the most common venom toxin transcripts To identify the most common toxin transcripts of each toxin family from C. atrox crude venom, we adopted the procedure developed in our lab ( ) with some minor modifications: i) we used pJET1.2 (ThermoFisher Scientific, USA) as the cloning vector; and ii) we divided PCR product (20μl) of each clone into two 10μl, one for running agarose gel to obtain the transcript with correct molecular size and the other 10μl for Alu I restriction enzyme digestion of same molecular size to acquire the most common transcript in terms of the enzyme digestion pattern. .. The recombinant plasmid DNAs isolated from the most common clones were sequenced.

    Article Title: One RNA plays three roles to provide catalytic activity to a group I intron lacking an endogenous internal guide sequence
    Article Snippet: .. The PCR products were cloned into the vector pJET1.2 (Fermentas) and transformed into Escherichia coli . .. Individual colonies were picked as templates for colony PCR reactions employing the primers pJET1.2-F and pJET1.2-R (Fermentas), which generate products of ∼310 bp [= the insert size (∼192 bp) plus about 120 bp].

    Article Title: The transcriptome of Candida albicans mitochondria and the evolution of organellar transcription units in yeasts
    Article Snippet: .. Cycling conditions were 98 °C/3 min, 28 cycles of 98 °C/ 30 s, 55 °C/30 s, 72 °C/30 s, 72 °C/7 min. PCR products were separated on 3 % high resolution agarose gels (Polskie Agarozy), excised, eluted from the gel (Thermo Scientific GeneJET kit) and cloned into pJET1.2 Cloning Vector (Thermo Scientific CloneJET). .. Bacterial clones containing the plasmids with the inserts of proper size were identified by Bgl II digestion.

    Transformation Assay:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: .. The purified fragments were ligated into a pJET1.2 cloning vector using a CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and transformed into competent E. coli (TOP10 cells). .. Positive clones were selected by colony PCR followed by plasmid isolation with the PrestoTM Mini Plasmid Kit (Geneaid).

    Article Title: One RNA plays three roles to provide catalytic activity to a group I intron lacking an endogenous internal guide sequence
    Article Snippet: .. The PCR products were cloned into the vector pJET1.2 (Fermentas) and transformed into Escherichia coli . .. Individual colonies were picked as templates for colony PCR reactions employing the primers pJET1.2-F and pJET1.2-R (Fermentas), which generate products of ∼310 bp [= the insert size (∼192 bp) plus about 120 bp].

    Recombinant:

    Article Title: Structure and Activity of a Cytosolic Ribosome-Inactivating Protein from Rice
    Article Snippet: .. Construct for Recombinant Protein Production The OsRIP1 coding sequence ligated in the pJET1.2 vector (Thermo Fisher Scientific) was used as a template to prepare the OsRIP1 His6-tagged expression construct. .. DNA sequences encoding a C-terminal Gly3-linker followed by a 6xHis-tag, as well as Gibson assembly sites allowing recombination into the pET21a vector by Gibson assembly, were added to the ORF by two consecutive PCRs.

    Plasmid Preparation:

    Article Title: Structure and Activity of a Cytosolic Ribosome-Inactivating Protein from Rice
    Article Snippet: .. Construct for Recombinant Protein Production The OsRIP1 coding sequence ligated in the pJET1.2 vector (Thermo Fisher Scientific) was used as a template to prepare the OsRIP1 His6-tagged expression construct. .. DNA sequences encoding a C-terminal Gly3-linker followed by a 6xHis-tag, as well as Gibson assembly sites allowing recombination into the pET21a vector by Gibson assembly, were added to the ORF by two consecutive PCRs.

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: .. The purified fragments were ligated into a pJET1.2 cloning vector using a CloneJET PCR Cloning Kit (Thermo Fisher Scientific) and transformed into competent E. coli (TOP10 cells). .. Positive clones were selected by colony PCR followed by plasmid isolation with the PrestoTM Mini Plasmid Kit (Geneaid).

    Article Title: Toxin transcripts in Crotalus atrox venom and in silico structures of toxins
    Article Snippet: .. Identification of the most common venom toxin transcripts To identify the most common toxin transcripts of each toxin family from C. atrox crude venom, we adopted the procedure developed in our lab ( ) with some minor modifications: i) we used pJET1.2 (ThermoFisher Scientific, USA) as the cloning vector; and ii) we divided PCR product (20μl) of each clone into two 10μl, one for running agarose gel to obtain the transcript with correct molecular size and the other 10μl for Alu I restriction enzyme digestion of same molecular size to acquire the most common transcript in terms of the enzyme digestion pattern. .. The recombinant plasmid DNAs isolated from the most common clones were sequenced.

    Article Title: CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing Fungus Lichtheimia corymbifera
    Article Snippet: .. The amplified fragment was ligated into the pJet1.2 cloning vector (Thermo Scientific, Waltham, MA, USA), arising the plasmid pLcpyrGcompl ( a), which was used to complement the uracil auxotrophy of the pyrG disrupted mutants. .. The plasmid was transformed into the mutants by PEG-mediated protoplast transformation.

    Article Title: One RNA plays three roles to provide catalytic activity to a group I intron lacking an endogenous internal guide sequence
    Article Snippet: .. The PCR products were cloned into the vector pJET1.2 (Fermentas) and transformed into Escherichia coli . .. Individual colonies were picked as templates for colony PCR reactions employing the primers pJET1.2-F and pJET1.2-R (Fermentas), which generate products of ∼310 bp [= the insert size (∼192 bp) plus about 120 bp].

    Article Title: The transcriptome of Candida albicans mitochondria and the evolution of organellar transcription units in yeasts
    Article Snippet: .. Cycling conditions were 98 °C/3 min, 28 cycles of 98 °C/ 30 s, 55 °C/30 s, 72 °C/30 s, 72 °C/7 min. PCR products were separated on 3 % high resolution agarose gels (Polskie Agarozy), excised, eluted from the gel (Thermo Scientific GeneJET kit) and cloned into pJET1.2 Cloning Vector (Thermo Scientific CloneJET). .. Bacterial clones containing the plasmids with the inserts of proper size were identified by Bgl II digestion.

    Article Title: Suggested Role for G4 DNA in Recombinational Switching at the Antigenic Variation Locus of the Lyme Disease Spirochete
    Article Snippet: .. Plasmid DNA was isolated using the Qiagen plasmid miniprep kit and sequenced by the University of Calgary Core DNA Services using the pJET1.2 forward/Custom sequencing primer (CloneJet, Fermentas). ..

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  • 90
    Thermo Fisher vector pjet1 2 blunt
    Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector <t>pJET1.2/blunt,</t> and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.
    Vector Pjet1 2 Blunt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pjet1 2 blunt/product/Thermo Fisher
    Average 90 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    vector pjet1 2 blunt - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    93
    Thermo Fisher pjet1 2 blunt cloning vector
    Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector <t>pJET1.2/blunt,</t> and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.
    Pjet1 2 Blunt Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pjet1 2 blunt cloning vector/product/Thermo Fisher
    Average 93 stars, based on 198 article reviews
    Price from $9.99 to $1999.99
    pjet1 2 blunt cloning vector - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.

    Journal: PLoS ONE

    Article Title: A Closer Look on the Polyhydroxybutyrate- (PHB-) Negative Phenotype of Ralstonia eutropha PHB-4

    doi: 10.1371/journal.pone.0095907

    Figure Lengend Snippet: Schematic presentation of the phaCAB operon of R. eutropha PHB - 4. Primers phaCAB_fw and phaCAB_rv were applied to generate the 4,860-bp DNA fragment comprising the phaCAB operon. The PCR fragment was subcloned into vector pJET1.2/blunt, and subsequently fragments of three independent hybrid plasmids were sequenced. The arrow marks the observed G320A mutation in phaC causing a stop codon obviously leading to a truncated and non functional PHA synthase (PhaC) in strain PHB - 4.

    Article Snippet: The purified PCR product (peqGOLD Gel Extraction Kit, peqlab) was ligated with vector pJET1.2/blunt and applied for transformation of competent E. coli TOP10 cells according to the CloneJET PCR Cloning Kit (Thermo Scientific).

    Techniques: Polymerase Chain Reaction, Plasmid Preparation, Mutagenesis, Functional Assay