vector pil253  (New England Biolabs)


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  • 99
    Name:
    Antarctic Phosphatase
    Description:
    Antarctic Phosphatase 5 000 units
    Catalog Number:
    M0289L
    Price:
    289
    Category:
    Alkaline Phosphatases
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs vector pil253
    Antarctic Phosphatase
    Antarctic Phosphatase 5 000 units
    https://www.bioz.com/result/vector pil253/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vector pil253 - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract"

    Article Title: Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

    Journal: Microbiology

    doi: 10.1099/mic.0.000515

    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.
    Figure Legend Snippet: Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Techniques Used: Western Blot, Hybridization, Marker

    Related Articles

    Plasmid Preparation:

    Article Title: speedingCARs: accelerating the engineering of CAR T cells by signaling domain shuffling and single-cell sequencing
    Article Snippet: .. The vector was digested with the Type IIS restriction enzyme AarI (Thermo Fisher) for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C. ..

    Article Title: Regulation of MHC-I and MHC-II by CIITA in transmissible cancers
    Article Snippet: .. Sleeping Beauty (SB) transposon plasmid pSBbi-BH ( ) (a gift from Eric Kowarz; Addgene # 60515, Cambridge, MA, USA) was digested at SfiI sites (NEB) with the addition of Antarctic Phosphatase (NEB) to prevent re-ligation. ..

    Article Title: Critical Role for Isoprenoids in Apicoplast Biogenesis by Malaria Parasites
    Article Snippet: .. Before transfection, the pMG74 vector was linearized by AflII digestion performed overnight at 37° C, followed by deactivation with Antarctic Phosphatase (NEB M0289S). ..

    Purification:

    Article Title: Efficient translation of Eggplant mottled dwarf nucleorhabdovirus N and X genes requires both 5′ and 3′ UTRs
    Article Snippet: .. 5′ UTR and 3′ UTR N gene transcripts were purified from agarose gels and dephosphorylated using Antarctic phosphatase (NEB). ..

    Article Title: Comparative Molecular Genomic Analyses of a Spontaneous Rhesus Macaque Model of Mismatch Repair-Deficient Colorectal Cancer
    Article Snippet: .. PCRs were performed using the Veriti 96 Well Thermal Cycler (Applied Biosystems®, Foster City, CA) under the following cycling conditions: initial denaturation at 95°C for 10 min, followed by 35 cycles at 95°C for 30 sec, 55°C for 30 sec and 72°C for 30 sec, with a final extension at 72°C for 7 min. Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml); 16 μl of Antarctic Phosphatase buffer (NEB® M0289S, Ipswich, MA); 144 μl of nuclease-free H2O]. ..

    Article Title: Genetic diversity and structure of the noble crayfish populations in the Balkan Peninsula revealed by mitochondrial and microsatellite DNA markers
    Article Snippet: .. The purification of PCR products was performed with EXOAnP Mix (20 U/µl of Exonuclease I (New England Biolabs), 5 U/µl of Antarctic phosphatase (New England Biolabs). ..

    Article Title: Direct reprogramming of human embryonic to trophoblast stem cells
    Article Snippet: .. RNA was phosphatase-treated using Antarctic phosphatase (New England BioLabs; Cat. No. M0289S) and purified using MEGAclear kit (Thermo Fisher; Cat. No. AM1909). ..

    Polymerase Chain Reaction:

    Article Title: Comparative Molecular Genomic Analyses of a Spontaneous Rhesus Macaque Model of Mismatch Repair-Deficient Colorectal Cancer
    Article Snippet: .. PCRs were performed using the Veriti 96 Well Thermal Cycler (Applied Biosystems®, Foster City, CA) under the following cycling conditions: initial denaturation at 95°C for 10 min, followed by 35 cycles at 95°C for 30 sec, 55°C for 30 sec and 72°C for 30 sec, with a final extension at 72°C for 7 min. Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml); 16 μl of Antarctic Phosphatase buffer (NEB® M0289S, Ipswich, MA); 144 μl of nuclease-free H2O]. ..

    Article Title: Complete genome sequence of a HPV31 isolate from laryngeal squamous cell carcinoma and biological consequences for p97 promoter activity
    Article Snippet: .. The PCR product was then dephosphorylated by incubation in the presence of 5 U Antarctic phosphatase (New England BioLabs, South Africa) for 30 minutes at 37°C. ..

    Article Title: Genetic diversity and structure of the noble crayfish populations in the Balkan Peninsula revealed by mitochondrial and microsatellite DNA markers
    Article Snippet: .. The purification of PCR products was performed with EXOAnP Mix (20 U/µl of Exonuclease I (New England Biolabs), 5 U/µl of Antarctic phosphatase (New England Biolabs). ..

    Incubation:

    Article Title: Complete genome sequence of a HPV31 isolate from laryngeal squamous cell carcinoma and biological consequences for p97 promoter activity
    Article Snippet: .. The PCR product was then dephosphorylated by incubation in the presence of 5 U Antarctic phosphatase (New England BioLabs, South Africa) for 30 minutes at 37°C. ..

    Transfection:

    Article Title: Critical Role for Isoprenoids in Apicoplast Biogenesis by Malaria Parasites
    Article Snippet: .. Before transfection, the pMG74 vector was linearized by AflII digestion performed overnight at 37° C, followed by deactivation with Antarctic Phosphatase (NEB M0289S). ..

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  • 99
    New England Biolabs vector pil253
    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), <t>pIL253</t> (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.
    Vector Pil253, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pil253/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vector pil253 - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

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    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Journal: Microbiology

    Article Title: Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

    doi: 10.1099/mic.0.000515

    Figure Lengend Snippet: Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Article Snippet: A 17 438 bp sequence containing the novel lantibiotic cluster was restricted from the identified pJAZZ-OC clone with ClaI and PstI (NEB) and then ligated into vector pIL253 [MspI, PstI restricted and dephosphorylated (Antarctic Phosphatase, NEB)] using Fastlink DNA ligase (Epicentre) to create p nso.

    Techniques: Western Blot, Hybridization, Marker