vector pil253  (New England Biolabs)


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    Structured Review

    New England Biolabs vector pil253
    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), <t>pIL253</t> (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.
    Vector Pil253, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pil253/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vector pil253 - by Bioz Stars, 2022-08
    97/100 stars

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    1) Product Images from "Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract"

    Article Title: Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

    Journal: Microbiology

    doi: 10.1099/mic.0.000515

    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.
    Figure Legend Snippet: Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Techniques Used: Western Blot, Hybridization, Marker

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    New England Biolabs vector pil253
    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), <t>pIL253</t> (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.
    Vector Pil253, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pil253/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vector pil253 - by Bioz Stars, 2022-08
    97/100 stars
      Buy from Supplier

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    Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Journal: Microbiology

    Article Title: Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract

    doi: 10.1099/mic.0.000515

    Figure Lengend Snippet: Western hybridization using the NsoA1 leader antibody to detect pre-peptide production in UKLc10. Comparison of L. lactis TCA-precipitated culture supernatant extracts (lanes 1–5) or cell extracts (lanes 6–11) from UKLc10 (lanes 1, 2, 4–8, 10 and 11) or MG1614 (lanes 3 and 9) containing plasmids p nsoA Δ A (lanes 1 and 11), p nsoA (lanes 2, 6 and 10), p nsoA Δ A , pTG nsoA3-nsoA4 (lanes 3 and 9), pIL253 (lanes 4 and 8) and p nsoA pTG nsoA3-nsoA4 (lanes 5 and 7). Samples were induced with nisin for 3 h, except for lane 6 (2 h). M, marker.

    Article Snippet: A 17 438 bp sequence containing the novel lantibiotic cluster was restricted from the identified pJAZZ-OC clone with ClaI and PstI (NEB) and then ligated into vector pIL253 [MspI, PstI restricted and dephosphorylated (Antarctic Phosphatase, NEB)] using Fastlink DNA ligase (Epicentre) to create p nso.

    Techniques: Western Blot, Hybridization, Marker