vectastain  (Vector Laboratories)


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    Name:
    VECTASTAIN ABC AmP Reagent for Western Blot Detection Standard Kit
    Description:
    VECTASTAIN AMP Amplifed Detection Greater sensitivity can be achieved by using an amplified detection procedure Our biotin avidin and biotin streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background The VECTASTAIN ABC AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes The signal can be visualized using either a chemiluminescent chemifluorescent or a chromogenic substrate Used with the DuoLuX Chemiluminescent Chemifluorescent Substrate the VECTASTAIN ABC AmP system produces a very high and sustained light emission signal with low background and permanent fluorescence The sensitivity of VECTASTAIN ABC AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein The VECTASTAIN ABC AmP Reagent is sufficient to develop approximately twenty 100 cm2blots
    Catalog Number:
    AK-6000
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories vectastain
    VECTASTAIN ABC AmP Reagent for Western Blot Detection Standard Kit
    VECTASTAIN AMP Amplifed Detection Greater sensitivity can be achieved by using an amplified detection procedure Our biotin avidin and biotin streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background The VECTASTAIN ABC AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes The signal can be visualized using either a chemiluminescent chemifluorescent or a chromogenic substrate Used with the DuoLuX Chemiluminescent Chemifluorescent Substrate the VECTASTAIN ABC AmP system produces a very high and sustained light emission signal with low background and permanent fluorescence The sensitivity of VECTASTAIN ABC AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein The VECTASTAIN ABC AmP Reagent is sufficient to develop approximately twenty 100 cm2blots
    https://www.bioz.com/result/vectastain/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain - by Bioz Stars, 2021-05
    98/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo
    Article Snippet: .. The SAF84 antibody was used to label PrPSc and the Vectastain ABC-AmP kit (Vector laboratories, USA) to reveal antibody binding. .. Softwares and statistical analyses Kaplan-Meier survival curves were done using the GraphPad Prism software (La Jolla, CA, USA).

    Article Title: A Fluorescent Oligothiophene-Bis-Triazine ligand interacts with PrP fibrils and detects SDS-resistant oligomers in human prion diseases
    Article Snippet: .. The SAF84 antibody was used to label rPrPSc and the Vectastain ABC-AmP kit (Vector laboratories, USA) to reveal antibody binding. .. Software and statistical analysis ImageJ ( http://rsb.info.nih.gov/ij/index.html ) was used to compare the intensity of individual western blot bands.

    Article Title: Plasma cholesterol level determines in vivo prion propagation [S]
    Article Snippet: .. The SAF84 antibody was used to label PrPSc and the Vectastain ABC-AmP kit (Vector Laboratories) was used to reveal antibody binding. ..

    FLAG-tag:

    Article Title: Selective incorporation of influenza virus RNA segments into virions
    Article Snippet: .. Antigens were detected with anti-FLAG monoclonal antibody M2 (Sigma) or rabbit antiserum against influenza WSN virus used as the primary antibody and biotinylated anti-mouse IgG for the FLAG epitope or biotinylated anti-rabbit IgG for viral antigens used as the secondary antibody (VECTASTAIN ABC kit, Vector Laboratories). .. Cells were fixed with 3% formaldehyde solution, permeated by 0.1% Triton X-100 in 3% formaldehyde solution, and prehybridized at 65°C for 30 min in prehybridization buffer [5× SSC (1× SSC = 0.15 M sodium chloride/0.015 M sodium citrate, pH 7.0)/1% blocking reagent/DIG nucleic acid-detection kit (Roche Diagnostics, Indianapolis)/0.1% N -lauroylsarcosine/0.02% SDS containing 0.1 mg/ml poly(A) DNA from the DIG-detection kit)].

    Incubation:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

    Article Title: The Hemagglutinin-Neuraminidase Protein of Newcastle Disease Virus Determines Tropism and Virulence
    Article Snippet: The sections were then incubated with a 1:500 dilution of the primary NDV monoclonal antibody ( ) cocktail against HN (10D11, AVS, 15C4, and B79) for 1 h at room temperature. .. After a wash, sections were incubated with the VectaStain secondary antibody (Vector Laboratories) for 30 min, as recommended by the manufacturer. .. After a further wash cycle, the sections were incubated with the VectaStain ABC reagent for 30 min.

    Staining:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

    Immunostaining:

    Article Title: Involvement of TRPC Channels in Lung Cancer Cell Differentiation and the Correlation Analysis in Human Non-Small Cell Lung Cancer
    Article Snippet: .. The immunostaining procedure was similar to our reports , and the VECTASTAIN ABC system (Vector Laboratories, Peterborough, UK) was used. .. The rabbit anti-TRPC1, 3, 4 and 6 antibodies purchased from Abcam (Cambridge, UK) were used for human lung tissue and lung cancer section staining.

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  • 98
    Vector Laboratories vectastain abc kit
    Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the <t>VECTASTAIN</t> <t>ABC</t> kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).
    Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc kit/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain abc kit - by Bioz Stars, 2021-05
    98/100 stars
      Buy from Supplier

    99
    Vector Laboratories immunoperoxidase kit
    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive <t>immunoperoxidase</t> staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).
    Immunoperoxidase Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoperoxidase kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoperoxidase kit - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin peroxidase complex method
    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, <t>avidin-biotin-peroxidase-complex</t> <t>method,</t> 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Avidin Biotin Peroxidase Complex Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex method/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex method - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the VECTASTAIN ABC kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Selective incorporation of influenza virus RNA segments into virions

    doi: 10.1073/pnas.0437772100

    Figure Lengend Snippet: Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the VECTASTAIN ABC kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).

    Article Snippet: Antigens were detected with anti-FLAG monoclonal antibody M2 (Sigma) or rabbit antiserum against influenza WSN virus used as the primary antibody and biotinylated anti-mouse IgG for the FLAG epitope or biotinylated anti-rabbit IgG for viral antigens used as the secondary antibody (VECTASTAIN ABC kit, Vector Laboratories).

    Techniques: Infection, Incubation, FLAG-tag, Immunostaining, Plasmid Preparation, Sequencing, In Situ Hybridization, Labeling

    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Journal: Infection and Immunity

    Article Title: Analysis of Pathogen-Host Cell Interactions in Purpura Fulminans: Expression of Capsule, Type IV Pili, and PorA by Neisseria meningitidis In Vivo

    doi: 10.1128/IAI.70.9.5193-5201.2002

    Figure Lengend Snippet: Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Article Snippet: Labeling was detected with an immunoperoxidase kit (Vectastain Elite ABC kit; Vector Laboratories, Burlingame, Calif.), and sections were counterstained in hematoxylin.

    Techniques: Immunohistochemistry, Staining, Immunoperoxidase Staining

    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Journal: Viruses

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    doi: 10.3390/v10120681

    Figure Lengend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Article Snippet: Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain.

    Techniques: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Journal: Journal of Clinical Investigation

    Article Title: Minimally modified low-density lipoprotein induces monocyte adhesion to endothelial connecting segment-1 by activating ?1 integrin

    doi:

    Figure Lengend Snippet: Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Article Snippet: Antibodies were viewed using ABC (catalog no. PK6100; Vector Laboratories) and AEC (catalog no. SO1; BiomedaFoster City, California, USA) kits.

    Techniques: Staining, Avidin-Biotin Assay