vectastain elite abc reagent  (Vector Laboratories)


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    Name:
    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    PK-7100
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    50 ml
    Buy from Supplier


    Structured Review

    Vector Laboratories vectastain elite abc reagent
    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/vectastain elite abc reagent/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc reagent - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "MUC16/CA125 in the Context of Modular Proteins with an Annotated Role in Adhesion-Related Processes: In Silico Analysis"

    Article Title: MUC16/CA125 in the Context of Modular Proteins with an Annotated Role in Adhesion-Related Processes: In Silico Analysis

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms130810387

    CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). Binding was detected using biotinylated goat anti-mouse IgG and Vectastain Elite ABC reagent. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).
    Figure Legend Snippet: CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). Binding was detected using biotinylated goat anti-mouse IgG and Vectastain Elite ABC reagent. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).

    Techniques Used: Binding Assay

    Related Articles

    Blocking Assay:

    Article Title: Antagonists targeting eEF2 kinase rescue multiple aspects of pathophysiology in Alzheimer’s disease model mice
    Article Snippet: .. Following a 15-minute blocking in 3% hydrogen peroxide, sections were incubated in biotinylated anti-mouse secondary antibody (1:200, Vector Labs) for 1 hour at room temperature, followed by Vectastain Elite ABC Reagent (Vector Labs) for another 30 minutes. ..

    Incubation:

    Article Title: Antagonists targeting eEF2 kinase rescue multiple aspects of pathophysiology in Alzheimer’s disease model mice
    Article Snippet: .. Following a 15-minute blocking in 3% hydrogen peroxide, sections were incubated in biotinylated anti-mouse secondary antibody (1:200, Vector Labs) for 1 hour at room temperature, followed by Vectastain Elite ABC Reagent (Vector Labs) for another 30 minutes. ..

    Article Title: Preliminary Evaluation of a Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Providing Full Protection against Heterologous Virulent Challenge in Cattle
    Article Snippet: .. The slides were incubated with 3% hydrogen peroxide and serum blocked per manufacturer instructions (VECTASTAIN Elite ABC-HRP Kit, Peroxidase (Rabbit IgG)–(PK-6101), Vector Labs (VL); Burlingame, CA, USA). ..

    Article Title: The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infection
    Article Snippet: .. After washing, slides were then incubated with Vectastain Elite ABC Reagent (Vector Laboratories). ..

    Article Title: Characterization of early Alzheimer’s-like pathological alterations in non-human primates with aging: a pilot study
    Article Snippet: .. Sections were then incubated in biotinylated α mouse (6E10) or rabbit (AT8) secondary antibody (1:200; Vector Labs, Burlingame, CA) for 2 hrs at room temperature followed by Vectastain Elite ABC reagent (Vector Labs) for another 30 minutes. ..

    Article Title: Chk1 and the Host Cell DNA Damage Response as a Potential Antiviral Target in BK Polyomavirus Infection
    Article Snippet: .. Slides were rinsed in PBS and incubated with preformed avidin:biotin enzyme complex (Vectastain Elite, Vector labs, Burlingame, CA, USA) followed by incubation with DAB substrate. ..

    Article Title: Function and Biomarkers of the Blood-Brain Barrier in a Neonatal Germinal Matrix Haemorrhage Model
    Article Snippet: .. In short, residual agarose was removed from the sections before endogenous peroxidase was blocked by incubation with 1% H2 O2 in PBS and incubated overnight with the Vectastain Elite ABC HRP kit (Vector Laboratories). ..

    Article Title: Cellular senescence promotes endothelial activation through epigenetic alteration, and consequently accelerates atherosclerosis
    Article Snippet: .. After washing with PBS, sections were incubated with biotinylated secondary antibody (Vector Laboratories, 1:300) for 1 h at room temperature, followed by incubation with VECTASTAIN Elite ABC-HRP reagent (Vector Laboratories) for 30 min. ..

    Avidin-Biotin Assay:

    Article Title: Chk1 and the Host Cell DNA Damage Response as a Potential Antiviral Target in BK Polyomavirus Infection
    Article Snippet: .. Slides were rinsed in PBS and incubated with preformed avidin:biotin enzyme complex (Vectastain Elite, Vector labs, Burlingame, CA, USA) followed by incubation with DAB substrate. ..

    Plasmid Preparation:

    Article Title: Early intrahepatic duct defects in a cystic fibrosis porcine model). Early intrahepatic duct defects in a cystic fibrosis porcine model
    Article Snippet: .. Secondary Ab (1:200, 30 min; Vector Biotinylated Anti‐Mouse IgG) was then applied followed by Vector ABC Reagent (30 min, Standard VECTASTAIN® Elite® ABC Kit, Vector Laboratories, Inc.) and chromogen (room temperature, DAB plus for 5 min followed by DAB Enhancer for 3 min). ..

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  • 99
    Vector Laboratories vectastain elite abc reagent
    CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). Binding was detected using biotinylated goat anti-mouse IgG and <t>Vectastain</t> Elite <t>ABC</t> reagent. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).
    Vectastain Elite Abc Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain elite abc reagent/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc reagent - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories horse anti mouse igg antibody
    Immunoreactivity and localization of forkhead box P3 <t>(FOXP3)+CD4+</t> cells. Double-staining using labeled streptavidin biotin (LSAB) detection with 3,3'-diaminobenzidine (DAB-brown) for FOXP3 expression and LSAB alkaline phosphatase detection with Fast Red substrate (red) for CD4+ cells was performed as described in the text (A). Symbols used: ☆, FOXP3+/CD4+ cell; △, FOXP3-/CD4+ cell. (B) Double-staining with anti-FOXP3 mAb and <t>IgG1</t> (negative control for CD4). The arrow indicates FOXP3+ cells. Immunoreactivity is evident for only FOXP3+ cells. There is no immunoreactivity for IgG1. (C) The negative control for both FOXP3 and CD4 shows no immunoreactivity for FOXP3 or CD4. Magnification ×400 HPF
    Horse Anti Mouse Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse anti mouse igg antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse anti mouse igg antibody - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    98
    Vector Laboratories vectastain elite abc kit
    Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2 . For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, <t>Vectastain</t> Elite <t>ABC</t> reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt's”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (∗) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).
    Vectastain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain elite abc kit/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc kit - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). Binding was detected using biotinylated goat anti-mouse IgG and Vectastain Elite ABC reagent. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).

    Journal: International Journal of Molecular Sciences

    Article Title: MUC16/CA125 in the Context of Modular Proteins with an Annotated Role in Adhesion-Related Processes: In Silico Analysis

    doi: 10.3390/ijms130810387

    Figure Lengend Snippet: CA125-immunoreactivity of Herpesvirus antigens. Mouse monoclonal anti-human CA125 antibodies: clone X306 (OC125-like) and clone X325 (M-11 like) were allowed to react with immobilized Epstein-Barr Virus (EBV) capsid antigens or Herpes simplex virus type 1 antigens (HSV1). Binding was detected using biotinylated goat anti-mouse IgG and Vectastain Elite ABC reagent. The absorbance was measured at 450 nm. Non-specific binding was estimated using an irrelevant monoclonal anti-hCG antibody (c).

    Article Snippet: Subsequent to incubation for 1 h, the wells were rinsed and Vectastain Elite ABC reagent (Vector Laboratories, Burlinghame, CA, USA) was added followed by incubation for 30 min. After another washing step, addition of TMB substrate solution and incubation for 10 min, the reaction was stopped with 0.16 M H2 SO4 .

    Techniques: Binding Assay

    Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis . Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).

    Journal: BMC Gastroenterology

    Article Title: Caerulein-induced acute pancreatitis in mice that constitutively overexpress Reg/PAP genes

    doi: 10.1186/1471-230X-6-16

    Figure Lengend Snippet: Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis . Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).

    Article Snippet: Paraffin sections (5 μm) of pancreas were processed for immunohistochemistry of Reg3α using the Vectastain ABC technique (Vector Labs, Burlingame, CA, USA).

    Techniques: Immunohistochemistry, Mouse Assay, Staining, Injection, Expressing, Negative Control

    Immunoreactivity and localization of forkhead box P3 (FOXP3)+CD4+ cells. Double-staining using labeled streptavidin biotin (LSAB) detection with 3,3'-diaminobenzidine (DAB-brown) for FOXP3 expression and LSAB alkaline phosphatase detection with Fast Red substrate (red) for CD4+ cells was performed as described in the text (A). Symbols used: ☆, FOXP3+/CD4+ cell; △, FOXP3-/CD4+ cell. (B) Double-staining with anti-FOXP3 mAb and IgG1 (negative control for CD4). The arrow indicates FOXP3+ cells. Immunoreactivity is evident for only FOXP3+ cells. There is no immunoreactivity for IgG1. (C) The negative control for both FOXP3 and CD4 shows no immunoreactivity for FOXP3 or CD4. Magnification ×400 HPF

    Journal: Allergy, Asthma & Immunology Research

    Article Title: Decreased Expression of FOXP3 in Nasal Polyposis

    doi: 10.4168/aair.2012.4.1.24

    Figure Lengend Snippet: Immunoreactivity and localization of forkhead box P3 (FOXP3)+CD4+ cells. Double-staining using labeled streptavidin biotin (LSAB) detection with 3,3'-diaminobenzidine (DAB-brown) for FOXP3 expression and LSAB alkaline phosphatase detection with Fast Red substrate (red) for CD4+ cells was performed as described in the text (A). Symbols used: ☆, FOXP3+/CD4+ cell; △, FOXP3-/CD4+ cell. (B) Double-staining with anti-FOXP3 mAb and IgG1 (negative control for CD4). The arrow indicates FOXP3+ cells. Immunoreactivity is evident for only FOXP3+ cells. There is no immunoreactivity for IgG1. (C) The negative control for both FOXP3 and CD4 shows no immunoreactivity for FOXP3 or CD4. Magnification ×400 HPF

    Article Snippet: Specific immunoreactivity of the anti-FOXP3 antibodies was visualized by incubating the tissues in multiple-adsorption biotinylated secondary horse anti-mouse IgG antibody (1:200 dilution, Vectastain Elite ABC Reagent; Vector Laboratories) and the 3-amino-9-ethyl carbazole (AEC) substrate chromogen (Ready to Use kit; Dako).

    Techniques: Double Staining, Labeling, Expressing, Negative Control

    Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2 . For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, Vectastain Elite ABC reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt's”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (∗) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).

    Journal: AIDS Research and Treatment

    Article Title: Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma

    doi: 10.1155/2010/164586

    Figure Lengend Snippet: Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2 . For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, Vectastain Elite ABC reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt's”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (∗) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).

    Article Snippet: Next, a biotinylated secondary antibody (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) was applied, followed by Vectastain Elite ABC reagent.

    Techniques: Expressing, Immunohistochemistry, Staining, Negative Control, Microscopy