vectastain elite abc kit  (Vector Laboratories)


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    Name:
    VECTASTAIN ABC AmP Reagent for Western Blot Detection Standard Kit
    Description:
    VECTASTAIN AMP Amplifed Detection Greater sensitivity can be achieved by using an amplified detection procedure Our biotin avidin and biotin streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background The VECTASTAIN ABC AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes The signal can be visualized using either a chemiluminescent chemifluorescent or a chromogenic substrate Used with the DuoLuX Chemiluminescent Chemifluorescent Substrate the VECTASTAIN ABC AmP system produces a very high and sustained light emission signal with low background and permanent fluorescence The sensitivity of VECTASTAIN ABC AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein The VECTASTAIN ABC AmP Reagent is sufficient to develop approximately twenty 100 cm2blots
    Catalog Number:
    AK-6000
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories vectastain elite abc kit
    VECTASTAIN ABC AmP Reagent for Western Blot Detection Standard Kit
    VECTASTAIN AMP Amplifed Detection Greater sensitivity can be achieved by using an amplified detection procedure Our biotin avidin and biotin streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background The VECTASTAIN ABC AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes The signal can be visualized using either a chemiluminescent chemifluorescent or a chromogenic substrate Used with the DuoLuX Chemiluminescent Chemifluorescent Substrate the VECTASTAIN ABC AmP system produces a very high and sustained light emission signal with low background and permanent fluorescence The sensitivity of VECTASTAIN ABC AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein The VECTASTAIN ABC AmP Reagent is sufficient to develop approximately twenty 100 cm2blots
    https://www.bioz.com/result/vectastain elite abc kit/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain elite abc kit - by Bioz Stars, 2021-09
    98/100 stars

    Images

    1) Product Images from "Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma"

    Article Title: Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma

    Journal: AIDS Research and Treatment

    doi: 10.1155/2010/164586

    Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2 . For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, Vectastain Elite ABC reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt's”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (∗) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).
    Figure Legend Snippet: Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2 . For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, Vectastain Elite ABC reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt's”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (∗) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).

    Techniques Used: Expressing, Immunohistochemistry, Staining, Negative Control, Microscopy

    2) Product Images from "Regulation of Macrophage Foam Cell Formation During Nitrogen Mustard (NM)-Induced Pulmonary Fibrosis by Lung Lipids"

    Article Title: Regulation of Macrophage Foam Cell Formation During Nitrogen Mustard (NM)-Induced Pulmonary Fibrosis by Lung Lipids

    Journal: Toxicological Sciences

    doi: 10.1093/toxsci/kfz187

    Effects of NM on lung lipids. A, Frozen tissue sections and cytospins, prepared 28 days after exposure of rats to NM or to PBS control (CTL), were stained with Oil Red O. Arrows, cells in insets. Original magnification: ×100; inset magnification: ×600. Representative images from at least 3 rats/treatment group are shown. B, BAL was collected 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL). Large aggregate fractions were prepared and assayed for phospholipid content as described in Materials and Methods . Bars, mean ± SE (n = 3–5 rats). *Significantly different ( p ≤ .05) from CTL. C, Tissue sections, prepared 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL), were immunostained with antibody to oxidized phospholipids. Binding was visualized using a Vectastain kit. Arrows, macrophages in insets. Original magnification, ×100; inset magnification, ×1000. Representative sections from 3 rats/treatment group are shown.
    Figure Legend Snippet: Effects of NM on lung lipids. A, Frozen tissue sections and cytospins, prepared 28 days after exposure of rats to NM or to PBS control (CTL), were stained with Oil Red O. Arrows, cells in insets. Original magnification: ×100; inset magnification: ×600. Representative images from at least 3 rats/treatment group are shown. B, BAL was collected 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL). Large aggregate fractions were prepared and assayed for phospholipid content as described in Materials and Methods . Bars, mean ± SE (n = 3–5 rats). *Significantly different ( p ≤ .05) from CTL. C, Tissue sections, prepared 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL), were immunostained with antibody to oxidized phospholipids. Binding was visualized using a Vectastain kit. Arrows, macrophages in insets. Original magnification, ×100; inset magnification, ×1000. Representative sections from 3 rats/treatment group are shown.

    Techniques Used: Staining, Binding Assay

    Effects of NM on expression of ABCA1, FXR, and CD36. Lung sections, prepared 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL), were immunostained with antibody to ABCA1, FXR, and CD36. Binding was visualized using a Vectastain kit. Arrows, macrophages in insets. Original magnification, ×600; inset magnification, ×1000. Representative sections from 3 rats/treatment group are shown.
    Figure Legend Snippet: Effects of NM on expression of ABCA1, FXR, and CD36. Lung sections, prepared 3, 7, and 28 days after exposure of rats to NM or to PBS control (CTL), were immunostained with antibody to ABCA1, FXR, and CD36. Binding was visualized using a Vectastain kit. Arrows, macrophages in insets. Original magnification, ×600; inset magnification, ×1000. Representative sections from 3 rats/treatment group are shown.

    Techniques Used: Expressing, Binding Assay

    Related Articles

    Incubation:

    Article Title: A comprehensive structural, lectin and immunohistochemical characterization of the zebrafish olfactory system
    Article Snippet: .. The next day, the samples were (iv) 1.5 h incubation in Vectastain ABC reagent (Vector Laboratories, Burlingame, CA, USA). ..

    Article Title: Repositioning metformin and propranolol for colorectal and triple negative breast cancers treatment
    Article Snippet: .. Tissue slides were rinsed with TBS-Tween and incubated for 30 min with secondary antibody (Vectastain Elite ABC kit; Vector Laboratories, USA) then developed with diaminobenzidine (BD Pharmigen) and counterstained with hematoxylin. ..

    Staining:

    Article Title: Lentivirus vector-mediated genetic manipulation of oncogenic pathways induces tumor formation in rabbit brain
    Article Snippet: .. The staining was carried out with Vectastain ABC Method based on avidin-biotin-HRP reaction (Vector Laboratories, Inc.) with DAB substrate. ..

    Article Title: Melatonin Alleviated Potassium Dichromate-Induced Oxidative Stress and Reprotoxicity in Male Rats
    Article Snippet: .. Biotinylated polyvalent secondary antibody (Vectastain Elite ABC Kit; Vector Laboratories, Burlingame, CA) was incubated with tissue sections for 30 min. To visualize bound antibodies, sections were washed in PBS and covered with a 3,3′-diaminobenzidine substrate and incubated for 10 min. Counterstaining was performed by hematoxylin stain. ..

    Article Title: Salidroside promoted osteogenic differentiation of adipose-derived stromal cells through Wnt/β-catenin signaling pathway
    Article Snippet: .. Staining was performed using BCIP/NBT chromogenic substrate (Vectastain ABC-AmP Kit, Vector Laboratories). ..

    Avidin-Biotin Assay:

    Article Title: Lentivirus vector-mediated genetic manipulation of oncogenic pathways induces tumor formation in rabbit brain
    Article Snippet: .. The staining was carried out with Vectastain ABC Method based on avidin-biotin-HRP reaction (Vector Laboratories, Inc.) with DAB substrate. ..

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    Vector Laboratories avidin biotin peroxidase complex method
    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, <t>avidin-biotin-peroxidase-complex</t> <t>method,</t> 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Avidin Biotin Peroxidase Complex Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex method/product/Vector Laboratories
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    99
    Vector Laboratories immunoperoxidase kit
    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive <t>immunoperoxidase</t> staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).
    Immunoperoxidase Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    99
    Vector Laboratories vectastain elite abc
    Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using <t>Vectastain</t> Elite <t>ABC</t> and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.
    Vectastain Elite Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    Vector Laboratories biotinylated goat anti rabbit igg
    Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. <t>Biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Journal: Viruses

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    doi: 10.3390/v10120681

    Figure Lengend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Article Snippet: Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain.

    Techniques: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Journal: Infection and Immunity

    Article Title: Analysis of Pathogen-Host Cell Interactions in Purpura Fulminans: Expression of Capsule, Type IV Pili, and PorA by Neisseria meningitidis In Vivo

    doi: 10.1128/IAI.70.9.5193-5201.2002

    Figure Lengend Snippet: Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Article Snippet: Labeling was detected with an immunoperoxidase kit (Vectastain Elite ABC kit; Vector Laboratories, Burlingame, Calif.), and sections were counterstained in hematoxylin.

    Techniques: Immunohistochemistry, Staining, Immunoperoxidase Staining

    Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using Vectastain Elite ABC and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.

    Journal: Journal of Clinical Investigation

    Article Title: Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53

    doi:

    Figure Lengend Snippet: Immunohistochemical and subcellular localization of transgenic survivin expression. ( a ) Five-micrometer tissue sections were cut from fresh-frozen skin of K14-survivin transgenic mice (K14-survivin) or control nontransgenic (non-TG) littermates, fixed in ice-cold acetone, and analyzed by immunohistochemistry with Ab against survivin or a control Ab to EGFP. Binding of the primary Ab’s was visualized with a goat anti-rabbit polyclonal Ab using Vectastain Elite ABC and AEC peroxidase substrate kits. ( b ) Subcellular localization of transgenic survivin. Keratinocytes isolated from K14-survivin transgenic mice (K14-survivin) or nontransgenic littermates (non-TG) were adhered to glass coverslips, fixed in methanol-acetone, and incubated with an Ab to survivin, followed by Texas red–conjugated goat anti-rabbit Ab. Nuclei were stained with Hoechst 33342. Image merging analysis is shown.

    Article Snippet: Binding of the primary Ab was visualized using a goat anti-rabbit polyclonal Ab (1:100) and Vectastain Elite ABC and 3-amino-9-ethyl carbazole (AEC) peroxidase substrate kits (Vector Laboratories) according to the manufacturer’s instructions.

    Techniques: Immunohistochemistry, Transgenic Assay, Expressing, Mouse Assay, Binding Assay, Isolation, Incubation, Staining

    Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Detection of GNAQ in TG neurons during latency-reactivation cycle. TG were collected from 3 uninfected calves (U) (A), latently infected calves (L) (B), or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (DEX) (C). Thin sections were cut from formalin-fixed, paraffin-embedded TG sections. The GNAQ antibody (ab75825; Abcam) was diluted 1:450. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Arrows denote GNAQ + neurons in the respective samples. (D) The percentage of GNAQ-positive neurons from 296 uninfected neurons, 241 latently infected neurons, and 209 TG neurons at 6 h after latently infected calves were treated with DEX. An asterisk denotes significant differences ( P

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Infection, Formalin-fixed Paraffin-Embedded, Plasmid Preparation

    Akt3 is frequently detected in ORF2 + neurons during latency. Consecutive sections from formalin-fixed paraffin-embedded TG sections from latently infected calves were prepared. One section was stained with the Akt3 antibody (ab152157; Abcam) that was diluted 1:500. A consecutive section was stained with a peptide-specific ORF2 antibody (1:500 dilution). Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody for the sections. Areas of sections that contained ORF2 + neurons were subsequently examined for Akt3 staining. Numbers denote the ORF2-positive neurons, and neurons 1, 3, 4, and 5 were also Akt3 + . Magnification is approximately 400×, and these sections are representative of many sections that were examined.

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Akt3 is frequently detected in ORF2 + neurons during latency. Consecutive sections from formalin-fixed paraffin-embedded TG sections from latently infected calves were prepared. One section was stained with the Akt3 antibody (ab152157; Abcam) that was diluted 1:500. A consecutive section was stained with a peptide-specific ORF2 antibody (1:500 dilution). Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody for the sections. Areas of sections that contained ORF2 + neurons were subsequently examined for Akt3 staining. Numbers denote the ORF2-positive neurons, and neurons 1, 3, 4, and 5 were also Akt3 + . Magnification is approximately 400×, and these sections are representative of many sections that were examined.

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Formalin-fixed Paraffin-Embedded, Infection, Staining, Plasmid Preparation

    Comparison of Akt3 expression during the BoHV-1 latency-reactivation cycle. (A) TG were collected from 3 uninfected calves, 3 latently infected calves, or 3 latently infected calves treated with DEX for 6 h to initiate reactivation from latency. Thin sections were cut from formalin-fixed paraffin-embedded TG sections. The Akt3 antibody (ab152157; Abcam) was diluted 1:500. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Blue arrows denote Akt3-positive TG neurons that contained an Akt3-positive nucleus and a counterstained nucleolus. Black arrows denote TG neurons in which the nucleus was not visible, but they were Akt3 + . Closed circles denote TG neurons that contain a nucleus in which the nucleolus is counterstained but was not stained by the Akt3 antibody. These images are representative of many sections stained with the Akt3 antibody. Magnification is approximately 400×. (B) The percentage of Akt3-positive TG neurons from 500 total neurons was estimated from sections derived from 3 latently infected calves, 3 mock-infected calves, and 3 latently infected calves treated with DEX for 6 h. An asterisk denotes significant differences ( P

    Journal: Journal of Virology

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency

    doi: 10.1128/JVI.01937-17

    Figure Lengend Snippet: Comparison of Akt3 expression during the BoHV-1 latency-reactivation cycle. (A) TG were collected from 3 uninfected calves, 3 latently infected calves, or 3 latently infected calves treated with DEX for 6 h to initiate reactivation from latency. Thin sections were cut from formalin-fixed paraffin-embedded TG sections. The Akt3 antibody (ab152157; Abcam) was diluted 1:500. Biotinylated goat anti-rabbit IgG (Vector Laboratories) was used as a secondary antibody. Blue arrows denote Akt3-positive TG neurons that contained an Akt3-positive nucleus and a counterstained nucleolus. Black arrows denote TG neurons in which the nucleus was not visible, but they were Akt3 + . Closed circles denote TG neurons that contain a nucleus in which the nucleolus is counterstained but was not stained by the Akt3 antibody. These images are representative of many sections stained with the Akt3 antibody. Magnification is approximately 400×. (B) The percentage of Akt3-positive TG neurons from 500 total neurons was estimated from sections derived from 3 latently infected calves, 3 mock-infected calves, and 3 latently infected calves treated with DEX for 6 h. An asterisk denotes significant differences ( P

    Article Snippet: The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber.

    Techniques: Expressing, Infection, Formalin-fixed Paraffin-Embedded, Plasmid Preparation, Staining, Derivative Assay