vectastain abc kit  (Vector Laboratories)


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    Name:
    VECTASTAIN ABC AP Staining KIT Alkaline Phosphatase Standard
    Description:
    VECTASTAIN ABC AP Staining Kits remains widely popular with more than 50 000 citations to its credit Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory The sensitivity of the VECTASTAIN ABC AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system The VECTASTAIN ABC AP Kits may be preferred for tissues that have high endogenous peroxidase activity The system also offers additional substrate color choices VECTASTAIN ABC AP Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC AP complex Kit is sufficient to stain approximately 1000 2000 sections or fifty 100 cm2 blots
    Catalog Number:
    AK-5000
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories vectastain abc kit
    VECTASTAIN ABC AP Staining KIT Alkaline Phosphatase Standard
    VECTASTAIN ABC AP Staining Kits remains widely popular with more than 50 000 citations to its credit Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory The sensitivity of the VECTASTAIN ABC AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system The VECTASTAIN ABC AP Kits may be preferred for tissues that have high endogenous peroxidase activity The system also offers additional substrate color choices VECTASTAIN ABC AP Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC AP complex Kit is sufficient to stain approximately 1000 2000 sections or fifty 100 cm2 blots
    https://www.bioz.com/result/vectastain abc kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain abc kit - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: In case the primary antibody was biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted. .. In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase. .. The procedure is similar with the staining steps for frozen sections described above, except quenching of endogenous peroxidase is no longer required.

    Article Title: Induction of cyclooxygenase-2 in monocyte/macrophage by mucins secreted from colon cancer cells
    Article Snippet: .. Immunohistochemical staining was performed with the Vectastain avidin–biotin peroxidase complex kit (Vector Laboratories) as described ( ). .. Double immunostaining was carried out according to Kawahito et al. ( ).

    Article Title: Diet-induced obesity links to ER positive breast cancer progression via LPA/PKD-1-CD36 signaling-mediated microvascular remodeling
    Article Snippet: E0771 cells (1×106 cells/mouse) were implanted into mice close to the fourth mammary pad [ ] and tumor volume was measured using calipers in two dimensions and calculated using the formula: (width2 × length)/2 [ ]. .. Immunofluorescence and immunohistochemical assays Tissue immunohistochemical and immunofluorescence staining was performed using antibodies and methods as described in our previous studies [ , , ] or by using the Vectastain® ABC kit (Vector Laboratories). .. Mouse angiogenesis profiling A Proteome Profiler™ antibody array (R & D system® ) was performed to determine relative protein levels of angiogenic factors in the plasma according to the manufacturer's instruction.

    Article Title: Dynamic Populations of Dendritic Cell-Specific ICAM-3 Grabbing Nonintegrin-Positive Immature Dendritic Cells and Liver/Lymph Node-Specific ICAM-3 Grabbing Nonintegrin-Positive Endothelial Cells in the Outer Zones of the Paracortex of Human Lymph Nodes
    Article Snippet: Paraffin sections were rehydrated and subjected to antigen-retrieval by boiling in 0.01 mol/L citric acid (pH 6.0) for 10 minutes before incubation with antibodies. .. Staining was performed with the ABC-AP Vectastain kit (Vector Laboratories) or ABC-PO and diaminobenzidine tetrahydrochloride (0.5 mg/ml) and sections were counterstained with hematoxylin according to Pappanicolau. .. For immunofluorescence, Alexa 488 (Molecular Probes, Eugene, OR)- and Texas Red (Jackson, West Grove, PA)-conjugated secondary antibodies were used.

    Labeling:

    Article Title: Analysis of Pathogen-Host Cell Interactions in Purpura Fulminans: Expression of Capsule, Type IV Pili, and PorA by Neisseria meningitidis In Vivo
    Article Snippet: Briefly, sections were incubated in 0.3% aqueous hydrogen peroxide to quench endogenous peroxidase activity and blocked with normal mouse or rabbit serum before incubation with the monoclonal antibodies for 1 h at room temperature. .. Labeling was detected with an immunoperoxidase kit (Vectastain Elite ABC kit; Vector Laboratories, Burlingame, Calif.), and sections were counterstained in hematoxylin. .. Antigen retrieval treatment of the sections was required for optimal staining with all of the meningococcal monoclonal antibodies and the anti-CD31 and -CD68 antibodies ( ).

    Incubation:

    Article Title: Luteolin Inhibits Human Prostate Tumor Growth by Suppressing Vascular Endothelial Growth Factor Receptor 2-Mediated Angiogenesis
    Article Snippet: Histology and Immunohistochemistry Tumor tissues were fixed in 10% neutral-buffered formalin for 24 hours, processed, and embedded in paraffin blocks. .. The sections (5 µm) were blocked with 10% goat serum and incubated with a rabbit anti-CD31 (1∶100; Novus Biologicals Inc, Littleton, CO) and mouse ant-CD34 (1∶100; BD Pharmingen Inc, San Diego, CA) antibodies for 24 h. The slides were subsequently incubated for 30 min with biotinylated anti-rabbit/anti-mouse secondary antibody (Vector laboratories, Burlingame, CA) and followed by incubation of Vectastain ABC Kit (Vector Laboratories). .. Diaminobenzidine (Sigma) was used as the chromagen and methyl green (Sigma) as the counterstain.

    Immunohistochemistry:

    Article Title: Induction of cyclooxygenase-2 in monocyte/macrophage by mucins secreted from colon cancer cells
    Article Snippet: .. Immunohistochemical staining was performed with the Vectastain avidin–biotin peroxidase complex kit (Vector Laboratories) as described ( ). .. Double immunostaining was carried out according to Kawahito et al. ( ).

    Article Title: Diet-induced obesity links to ER positive breast cancer progression via LPA/PKD-1-CD36 signaling-mediated microvascular remodeling
    Article Snippet: E0771 cells (1×106 cells/mouse) were implanted into mice close to the fourth mammary pad [ ] and tumor volume was measured using calipers in two dimensions and calculated using the formula: (width2 × length)/2 [ ]. .. Immunofluorescence and immunohistochemical assays Tissue immunohistochemical and immunofluorescence staining was performed using antibodies and methods as described in our previous studies [ , , ] or by using the Vectastain® ABC kit (Vector Laboratories). .. Mouse angiogenesis profiling A Proteome Profiler™ antibody array (R & D system® ) was performed to determine relative protein levels of angiogenic factors in the plasma according to the manufacturer's instruction.

    Avidin-Biotin Assay:

    Article Title: Induction of cyclooxygenase-2 in monocyte/macrophage by mucins secreted from colon cancer cells
    Article Snippet: .. Immunohistochemical staining was performed with the Vectastain avidin–biotin peroxidase complex kit (Vector Laboratories) as described ( ). .. Double immunostaining was carried out according to Kawahito et al. ( ).

    Article Title: Bone Matrix Proteins: Isolation and Characterization of a Novel Cell-binding Keratan Sulfate Proteoglycan (Osteoadherin) from Bovine Bone
    Article Snippet: The sections were incubated with primary antibody against osteoadherin (diluted to 1:1,000 in PBS-0.01% BSA) or the preimmune serum (diluted to 1:1,000 in PBS 0.01% BSA) at 4°C overnight in a moist chamber. .. The sections were then treated with biotinylated secondary antibody (diluted 1:200) and avidin–peroxidase conjugate using the Vectastain ABC kit™ (Vector Labs, Burlingame, CA), following the recommendations of the manufacturer. .. Cell Attachment to Osteoadherin Primary osteoblasts isolated with the method of Robey et al. ( ) were grown to near confluency in Ham's F12 medium supplemented with 10% FBS, 50 UI penicillin, and 50 μg/ml streptomycin.

    Immunofluorescence:

    Article Title: Diet-induced obesity links to ER positive breast cancer progression via LPA/PKD-1-CD36 signaling-mediated microvascular remodeling
    Article Snippet: E0771 cells (1×106 cells/mouse) were implanted into mice close to the fourth mammary pad [ ] and tumor volume was measured using calipers in two dimensions and calculated using the formula: (width2 × length)/2 [ ]. .. Immunofluorescence and immunohistochemical assays Tissue immunohistochemical and immunofluorescence staining was performed using antibodies and methods as described in our previous studies [ , , ] or by using the Vectastain® ABC kit (Vector Laboratories). .. Mouse angiogenesis profiling A Proteome Profiler™ antibody array (R & D system® ) was performed to determine relative protein levels of angiogenic factors in the plasma according to the manufacturer's instruction.

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  • 98
    Vector Laboratories vectastain abc kit
    Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the <t>VECTASTAIN</t> <t>ABC</t> kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).
    Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc kit/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vectastain abc kit - by Bioz Stars, 2021-04
    98/100 stars
      Buy from Supplier

    99
    Vector Laboratories immunoperoxidase kit
    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive <t>immunoperoxidase</t> staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).
    Immunoperoxidase Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoperoxidase kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoperoxidase kit - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin peroxidase complex method
    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, <t>avidin-biotin-peroxidase-complex</t> <t>method,</t> 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Avidin Biotin Peroxidase Complex Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex method/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex method - by Bioz Stars, 2021-04
    99/100 stars
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    Image Search Results


    Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the VECTASTAIN ABC kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Selective incorporation of influenza virus RNA segments into virions

    doi: 10.1073/pnas.0437772100

    Figure Lengend Snippet: Confirmation of virus production with truncated NA RNA segments. ( A ) MDCK cells were infected with NA(−) ( a and d ), NAFLAG ( b and e ), or NAFLAGMet(−) ( c and f ) viruses and overlaid with 0.6% agarose. After incubation for 48 h at 37°C, the cells were fixed and permeated with 0.1% Triton X-100 in 3% formaldehyde solution. The viral proteins or FLAG epitope were detected by immunostaining with antiserum to influenza WSN strain ( a – c ) or anti-FLAG monoclonal antibody ( d – f ) as the primary antibody and biotinylated secondary antibody with the VECTASTAIN ABC kit (Vector Laboratories). ( B ) MDCK cells infected with NA(−), NAFLAG, or NAFLAGMet(−) viruses were incubated, fixed, and permeated as described above. The FLAG sequence in mRNA was detected by in situ hybridization with a digoxigenin-labeled probe specific for the sequence and visualized with the DIG nucleic acid-detection kit (Roche).

    Article Snippet: Antigens were detected with anti-FLAG monoclonal antibody M2 (Sigma) or rabbit antiserum against influenza WSN virus used as the primary antibody and biotinylated anti-mouse IgG for the FLAG epitope or biotinylated anti-rabbit IgG for viral antigens used as the secondary antibody (VECTASTAIN ABC kit, Vector Laboratories).

    Techniques: Infection, Incubation, FLAG-tag, Immunostaining, Plasmid Preparation, Sequencing, In Situ Hybridization, Labeling

    Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Journal: Infection and Immunity

    Article Title: Analysis of Pathogen-Host Cell Interactions in Purpura Fulminans: Expression of Capsule, Type IV Pili, and PorA by Neisseria meningitidis In Vivo

    doi: 10.1128/IAI.70.9.5193-5201.2002

    Figure Lengend Snippet: Immunohistochemical staining of inflammatory cells and the vascular endothelium in skin biopsy samples from patients with meningococcal disease. The inflammatory infiltrate consisted of a mixture of CD68-positive macrophages (A) (magnification, ×400) and neutrophil elastase-positive polymorphonuclear cells (B) (magnification, ×400). Multiple CD31-positive blood vessels were seen throughout the biopsy samples (C and D) (magnification, ×400 and 600, respectively). Arrows indicate positive immunoperoxidase staining (brown) with the appropriate specific mouse monoclonal antibody (nuclei were counterstained with hematoxylin).

    Article Snippet: Labeling was detected with an immunoperoxidase kit (Vectastain Elite ABC kit; Vector Laboratories, Burlingame, Calif.), and sections were counterstained in hematoxylin.

    Techniques: Immunohistochemistry, Staining, Immunoperoxidase Staining

    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Journal: Viruses

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    doi: 10.3390/v10120681

    Figure Lengend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Article Snippet: Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain.

    Techniques: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Journal: Journal of Clinical Investigation

    Article Title: Minimally modified low-density lipoprotein induces monocyte adhesion to endothelial connecting segment-1 by activating ?1 integrin

    doi:

    Figure Lengend Snippet: Human coronary lesions stained for CD 31, HAM 56, VCAM-1, 90.45, and HUTS-21. Human coronary lesions were stained with CD 31 ( a ), HAM 56 ( b ), 90.45 ( c ), or VCAM-1 ( d ). Antibodies in a–d were viewed with ABC and AEC. These four panels show that sections containing macrophages display endothelial CS-1 as detected by the 90.45 antibody but not VCAM-1 staining. In a separate study, the luminal endothelium of coronary vessels was stained for 90.45 ( e ) and HUTS-21 ( f ). Antibodies in e and f were viewed with DAB. Areas that stained most positively for 90.45 wer e mirrored by HUTS-21 ( arrow ), whereas areas of lesser staining were also parallel between the two antibodies ( double arrow ). ×1000. DAB, diaminobenzidine; ABC , avidin/biotinylated horseradish peroxide macromolecular complex; AEC , amino-9-ethyl carbazole.

    Article Snippet: Antibodies were viewed using ABC (catalog no. PK6100; Vector Laboratories) and AEC (catalog no. SO1; BiomedaFoster City, California, USA) kits.

    Techniques: Staining, Avidin-Biotin Assay