vectastain abc kit  (Vector Laboratories)


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    Vector Laboratories vectastain abc kit
    Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc kit/product/Vector Laboratories
    Average 99 stars, based on 2942 article reviews
    Price from $9.99 to $1999.99
    vectastain abc kit - by Bioz Stars, 2020-04
    99/100 stars

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    Amplification:

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: Western blots were developed using the Amplified AP Immun-Blot Kit (Bio-Rad). .. Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories).

    Blocking Assay:

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: Endogenous peroxidase activity was also quenched using BLOXALL blocking solution (Vector Labs; California, USA) for 10 min at room temperature. .. The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken
    Article Snippet: Paragraph title: Detection of H. meleagridis by ELISA and immunohistochemistry ... Following another washing step, the Vectastain ABC Kit and DAB Substrate Kit (Vector Laboratories) were used for visualizing H. meleagridis .

    Incubation:

    Article Title: Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken
    Article Snippet: After dewaxing and rehydration, samples were incubated overnight at 4 °C with a purified polyclonal anti-histomonad rabbit antibody. .. Following another washing step, the Vectastain ABC Kit and DAB Substrate Kit (Vector Laboratories) were used for visualizing H. meleagridis .

    Article Title: Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
    Article Snippet: Sections were incubated for 1 h at 4°C in a 3% normal donkey serum dissolved in 0.1 M phosphate buffered saline (PBS) and 0.3% TritonX-100 to prevent non-specific binding of IgGs. .. Goat anti-mouse (Chemicon, No. AP124B) or donkey anti-goat (Vectastain ABC kit, Vector, No. PK6105) or donkey anti-rabbit (Vectastain ABC kit, Vector, No. PK6101) biotynilated secondary antibody was used with a biotin-streptavidin immunostaining kit employing diamino benzidine (DAB) as a chromogen (Vectastaine Elite kit, Vector Laboratories).

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: Slides were incubated with the recombinant S1 spike proteins overnight at 4°C in a humidified chamber. .. The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs).

    Activity Assay:

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: Endogenous peroxidase activity was also quenched using BLOXALL blocking solution (Vector Labs; California, USA) for 10 min at room temperature. .. The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs).

    Cell Culture:

    Article Title: Potent Anti-Inflammatory and Neuroprotective Effects of TGF?1 Are Mediated through the Inhibition of ERK and p47phox-Ser345 Phosphorylation and Translocation in Microglia
    Article Snippet: Cell culture reagents were obtained from Invitrogen (Carlsbad, CA). .. The Vectastain ABC kit and biotinylated secondary antibodies were purchased from Vector Laboratories (Burlingame, CA).

    Expressing:

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Concentrations of se-To understand the complex interrelationships between rum antibodies were expressed as the highest dilution encephalitis, protection, and viral clearance leading to a with O.D. values three times above background level. persistent infection of the CNS, the expression of pro-Neutralizing antibodies were tested in serum as preand anti-inflammatory cytokine mRNAs in the CNS of viously described (Lin et al., 1997) . mice undergoing either lethal or sublethal JHMV infection were compared. .. Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) .

    Western Blot:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: Paragraph title: Western blot analysis ... Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs).

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: Purification of fAGP from pooled sera and evaluation of the pattern of sialylation AGP sialylation was characterised in a further Western blotting experiment using the biotin-conjugated lectins Sambucus nigra agglutinin (SNAI, 2 lg/mL; Vector Laboratories) and Maackia amurensis agglutinin (MAA, 5 lg/mL; Vector Laboratories) specific for sialic acid a(2-6)-linked and a(2-3)-linked to galactose, respectively. .. Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories).

    Derivative Assay:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: In this study protein derived from primary fibroblasts isolated from the caruncle (negative for cytokeratin) was included as well as the detection of a desmin protein specific band in placentome derived and fibroblast derived protein only. .. Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs).

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) . .. RNA was isolated from half brains by homogeni-activity was designated the 100% maximal response and values for the remainder were derived as percentage of zation at room temperature in guanidinium isothiocyanate using Tenbrock tissue homogenizers as previously the highest value.

    Immunohistochemistry:

    Article Title: Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken
    Article Snippet: Paragraph title: Detection of H. meleagridis by ELISA and immunohistochemistry ... Following another washing step, the Vectastain ABC Kit and DAB Substrate Kit (Vector Laboratories) were used for visualizing H. meleagridis .

    Article Title: Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
    Article Snippet: Paragraph title: Immunohistochemistry ... Goat anti-mouse (Chemicon, No. AP124B) or donkey anti-goat (Vectastain ABC kit, Vector, No. PK6105) or donkey anti-rabbit (Vectastain ABC kit, Vector, No. PK6101) biotynilated secondary antibody was used with a biotin-streptavidin immunostaining kit employing diamino benzidine (DAB) as a chromogen (Vectastaine Elite kit, Vector Laboratories).

    Concentration Assay:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: Briefly, protein was extracted with TRIzol-Reagent (Invitrogen, Karlsruhe, Germany), resuspended in a 1% sodium dodecyl sulphate (SDS) solution, diluted to a final concentration of 1000 mg/ml protein (AE10%) and stored at À20 C until further processing. .. Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs).

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: A concentration of 70 μg/mL of recombinant S1 spike proteins was used for each slide. .. The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs).

    Infection:

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Concentrations of se-To understand the complex interrelationships between rum antibodies were expressed as the highest dilution encephalitis, protection, and viral clearance leading to a with O.D. values three times above background level. persistent infection of the CNS, the expression of pro-Neutralizing antibodies were tested in serum as preand anti-inflammatory cytokine mRNAs in the CNS of viously described (Lin et al., 1997) . mice undergoing either lethal or sublethal JHMV infection were compared. .. Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) .

    Immunoperoxidase Staining:

    Article Title: Mouse hepatitis virus pathogenesis in the central nervous system is independent of IL-15 and natural killer cells.
    Article Snippet: .. Distribution of viral Ag was determined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratories, Burlingame, CA) using the anti-JHMV mAb J.3.3 specific for the carboxyl terminus of the viral nucleocapsid protein as primary Ab and horse anti-mouse secondary Ab (Vector Laboratories). ..

    Article Title: Expression of hemagglutinin/esterase by a mouse hepatitis virus coronavirus defective-interfering RNA alters viral pathogenesis.
    Article Snippet: .. The distribution of viral antigen was examined using immunoperoxidase staining (Vectastain-ABC kit, Vector Laboratories, Burlingame, CA) and anti-JHMV monoclonal antibody mAb J.3.3 specific for the carboxy terminus of the N protein (Fleming et al. 1983 ). ..

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: .. Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) . .. Radioactive signals of cytokine cDNA were quantified and normalized to the house-keeping enzyme hypoxanthine phosphoriboxyltransferase (HPRT) values to adjust Cytokine mRNA expression for differences in cDNA as previously described (Cua et al., 1995 (Cua et al., , 1996 .

    Binding Assay:

    Article Title: Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
    Article Snippet: Sections were incubated for 1 h at 4°C in a 3% normal donkey serum dissolved in 0.1 M phosphate buffered saline (PBS) and 0.3% TritonX-100 to prevent non-specific binding of IgGs. .. Goat anti-mouse (Chemicon, No. AP124B) or donkey anti-goat (Vectastain ABC kit, Vector, No. PK6105) or donkey anti-rabbit (Vectastain ABC kit, Vector, No. PK6101) biotynilated secondary antibody was used with a biotin-streptavidin immunostaining kit employing diamino benzidine (DAB) as a chromogen (Vectastaine Elite kit, Vector Laboratories).

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: .. Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories). ..

    Histopathology:

    Article Title: Mouse hepatitis virus pathogenesis in the central nervous system is independent of IL-15 and natural killer cells.
    Article Snippet: Paragraph title: Histopathology ... Distribution of viral Ag was determined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratories, Burlingame, CA) using the anti-JHMV mAb J.3.3 specific for the carboxyl terminus of the viral nucleocapsid protein as primary Ab and horse anti-mouse secondary Ab (Vector Laboratories).

    Article Title: Expression of hemagglutinin/esterase by a mouse hepatitis virus coronavirus defective-interfering RNA alters viral pathogenesis.
    Article Snippet: Paragraph title: Histopathology ... The distribution of viral antigen was examined using immunoperoxidase staining (Vectastain-ABC kit, Vector Laboratories, Burlingame, CA) and anti-JHMV monoclonal antibody mAb J.3.3 specific for the carboxy terminus of the N protein (Fleming et al. 1983 ).

    Nucleic Acid Electrophoresis:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: Gel electrophoresis and Western blotting were performed with the NuPAGE Gel System (Invitrogen) according to the manufacturer's protocol using 10% and 12% NuPAGE Novex Bis-Tris gels. .. Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs).

    Fluorescence:

    Article Title: Potent Anti-Inflammatory and Neuroprotective Effects of TGF?1 Are Mediated through the Inhibition of ERK and p47phox-Ser345 Phosphorylation and Translocation in Microglia
    Article Snippet: The Vectastain ABC kit and biotinylated secondary antibodies were purchased from Vector Laboratories (Burlingame, CA). .. The fluorescence probe Dichlorodihydro-fluorescein Diacetate (DCFH-DA) was obtained from Calbiochem (La Jolla, CA).

    Isolation:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: In this study protein derived from primary fibroblasts isolated from the caruncle (negative for cytokeratin) was included as well as the detection of a desmin protein specific band in placentome derived and fibroblast derived protein only. .. Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs).

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) . .. RNA was isolated from half brains by homogeni-activity was designated the 100% maximal response and values for the remainder were derived as percentage of zation at room temperature in guanidinium isothiocyanate using Tenbrock tissue homogenizers as previously the highest value.

    Immunodetection:

    Article Title: Bovine caruncular epithelial cell line (BCEC-1) isolated from the placenta forms a functional epithelial barrier in a polarised cell culture model.
    Article Snippet: .. Blotting on a nitrocellulose membrane (0.2 mm pore size) was followed by immunodetection (Vectastain ABC Kit, Vector Labs). ..

    Microscopy:

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs). .. Protein histochemistry Images of stained slides were taken with an Olympus BX41 microscope (New Jersey).

    Mouse Assay:

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Concentrations of se-To understand the complex interrelationships between rum antibodies were expressed as the highest dilution encephalitis, protection, and viral clearance leading to a with O.D. values three times above background level. persistent infection of the CNS, the expression of pro-Neutralizing antibodies were tested in serum as preand anti-inflammatory cytokine mRNAs in the CNS of viously described (Lin et al., 1997) . mice undergoing either lethal or sublethal JHMV infection were compared. .. Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) .

    Immunostaining:

    Article Title: Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
    Article Snippet: .. Goat anti-mouse (Chemicon, No. AP124B) or donkey anti-goat (Vectastain ABC kit, Vector, No. PK6105) or donkey anti-rabbit (Vectastain ABC kit, Vector, No. PK6101) biotynilated secondary antibody was used with a biotin-streptavidin immunostaining kit employing diamino benzidine (DAB) as a chromogen (Vectastaine Elite kit, Vector Laboratories). ..

    Purification:

    Article Title: Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken
    Article Snippet: After dewaxing and rehydration, samples were incubated overnight at 4 °C with a purified polyclonal anti-histomonad rabbit antibody. .. Following another washing step, the Vectastain ABC Kit and DAB Substrate Kit (Vector Laboratories) were used for visualizing H. meleagridis .

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: .. Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories). ..

    Plasmid Preparation:

    Article Title: Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
    Article Snippet: .. Goat anti-mouse (Chemicon, No. AP124B) or donkey anti-goat (Vectastain ABC kit, Vector, No. PK6105) or donkey anti-rabbit (Vectastain ABC kit, Vector, No. PK6101) biotynilated secondary antibody was used with a biotin-streptavidin immunostaining kit employing diamino benzidine (DAB) as a chromogen (Vectastaine Elite kit, Vector Laboratories). ..

    Software:

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories). .. Densitometric analysis was then performed using the Imagemaster 1D software (Amersham Biosciences) that analysed digital images of the membranes acquired with a scanner in order to display profile plots of fAGP peaks and to calculate the area under each peak.

    Recombinant:

    Article Title: Potent Anti-Inflammatory and Neuroprotective Effects of TGF?1 Are Mediated through the Inhibition of ERK and p47phox-Ser345 Phosphorylation and Translocation in Microglia
    Article Snippet: The recombinant human TGFβ1 was obtained from R & D system (Minneapolis, MN). .. The Vectastain ABC kit and biotinylated secondary antibodies were purchased from Vector Laboratories (Burlingame, CA).

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: Slides were incubated with the recombinant S1 spike proteins overnight at 4°C in a humidified chamber. .. The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs).

    Single Radial Immunodiffusion Assay:

    Article Title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
    Article Snippet: Purification of fAGP from pooled sera and evaluation of the pattern of sialylation After each step of the purification protocol, protein concentrations were quantified by spectrophotometric measurement at 280 nm and fAGP concentrations were determined by SRID. .. Purification of fAGP from pooled sera and evaluation of the pattern of sialylation Glycan analysis of fAGP-lectins binding was performed following a peroxidase reaction using the Vectastain ABC Kit (Vector Laboratories) as described previously (Ceciliani et al., 2004) and developed using the HRP Immun-Blot Assay Kit (Bio-Rad Laboratories).

    Standard Deviation:

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) . .. Data shown are mean values for 3-4 mice at each time point { 1 standard deviation. described (Cua et al., 1995) .

    Staining:

    Article Title: Mouse hepatitis virus pathogenesis in the central nervous system is independent of IL-15 and natural killer cells.
    Article Snippet: Deparaffinized sections were stained with hemotoxylin and eosin to determine inflammation or luxol fast blue for demyelination. .. Distribution of viral Ag was determined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratories, Burlingame, CA) using the anti-JHMV mAb J.3.3 specific for the carboxyl terminus of the viral nucleocapsid protein as primary Ab and horse anti-mouse secondary Ab (Vector Laboratories).

    Article Title: Expression of hemagglutinin/esterase by a mouse hepatitis virus coronavirus defective-interfering RNA alters viral pathogenesis.
    Article Snippet: At critical time points (e.g., day 3 p.i.) five step sections were taken through each hemisphere and stained for viral antigen. .. The distribution of viral antigen was examined using immunoperoxidase staining (Vectastain-ABC kit, Vector Laboratories, Burlingame, CA) and anti-JHMV monoclonal antibody mAb J.3.3 specific for the carboxy terminus of the N protein (Fleming et al. 1983 ).

    Article Title: Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.
    Article Snippet: Sections were stained with hematoxylin and eosin or luxol fast blue. .. Distri-SDS; room temperature), exposed to Storage Phosphor Screens (Molecular Dynamics, Sunnyvale, CA), and bution of JHMV antigen was examined by immunoperoxidase staining (Vectastain-ABC kit; Vector Laboratory, Burlin-scanned using a phosphorimaging scanner (Molecular Dynamics). game, CA) using the anti-JHMV mAb J3.3 specific for the viral nucleocapsid protein (Fleming et al., 1983) .

    Article Title: Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity.
    Article Snippet: The slides were then washed thrice with PBS and signal was developed using the Vectastain ABC kit (Vector Labs). .. Protein histochemistry Images of stained slides were taken with an Olympus BX41 microscope (New Jersey).

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    Vector Laboratories abc hrp vectastain kit
    Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in aorta of Ldlr −/− /E06-scFv mice a , Staining of atherosclerotic lesions of WHHL rabbit aorta with E06-scFv plasma (left panel), and Ldlr −/− mice (right panel) (both at dilution of 1:20), visualized using biotinylated anti-Myc mAb and <t>ABC-AP</t> <t>VectaStain</t> kit. b , Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst dye; Green, FITC-labeled anti-His tag mAb; Red, Annexin V-PE. c , Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+/Annexin V+) by FACS analysis. d , Expression of E06-scFv in aortic lesion of Ldlr −/− / E06-scFv but not Ldlr −/− mouse. Cross-sections at the AV were stained with biotinylated anti-Myc mAb to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using Hematoxylin QS (Original ×200). Panels a-c are representative of similar studies with 5 other plasma samples. Panel d is representative of studies in 3 other aortic sections.
    Abc Hrp Vectastain Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abc hrp vectastain kit/product/Vector Laboratories
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    abc hrp vectastain kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories vectastain abc ap kit
    Figure 6. Immunohistochemical detection of LC3B is feasible in liver from starved Gfp-Lc3 tg/tg mice using <t>Vectastain</t> <t>ABC.</t> ( A ) Western blot analysis of LC3B in liver from wild-type mice, heterozygous Gfp-Lc3 tg/+ or homozygous Gfp-Lc3 tg/tg transgenic mice. The GFP-LC3 expression in each group was quantified. **p
    Vectastain Abc Ap Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectastain abc ap kit/product/Vector Laboratories
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    vectastain abc ap kit - by Bioz Stars, 2020-04
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    Vector Laboratories pax7 cells
    Immunohistochemical determination of satellite cells per fiber (A), myonuclei per fiber (B), and relative satellite cell number [C; (satellite cells / satellite cells + myonuclei)]. (D) Transverse sections of mounted muscle were cut 6 μm thick from the midbelly of the gastrocnemius and soleus muscles and stained with antibodies against <t>Pax7.</t> Pax7-stained satellite cells were identified as any nuclei localized to the myofiber membrane and stained brown or with a brown rim (arrows). Sections were counterstained with hematoxylin to identify myonuclei, which are stained blue (arrow heads). Images were captured by light microscopy at 40× magnification and analyzed by a single investigator blinded to the sample. Values are mean ± SE. * Significantly greater than control within muscle (p
    Pax7 Cells, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pax7 cells/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pax7 cells - by Bioz Stars, 2020-04
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    Image Search Results


    Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in aorta of Ldlr −/− /E06-scFv mice a , Staining of atherosclerotic lesions of WHHL rabbit aorta with E06-scFv plasma (left panel), and Ldlr −/− mice (right panel) (both at dilution of 1:20), visualized using biotinylated anti-Myc mAb and ABC-AP VectaStain kit. b , Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst dye; Green, FITC-labeled anti-His tag mAb; Red, Annexin V-PE. c , Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+/Annexin V+) by FACS analysis. d , Expression of E06-scFv in aortic lesion of Ldlr −/− / E06-scFv but not Ldlr −/− mouse. Cross-sections at the AV were stained with biotinylated anti-Myc mAb to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using Hematoxylin QS (Original ×200). Panels a-c are representative of similar studies with 5 other plasma samples. Panel d is representative of studies in 3 other aortic sections.

    Journal: Nature

    Article Title: Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice

    doi: 10.1038/s41586-018-0198-8

    Figure Lengend Snippet: Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in aorta of Ldlr −/− /E06-scFv mice a , Staining of atherosclerotic lesions of WHHL rabbit aorta with E06-scFv plasma (left panel), and Ldlr −/− mice (right panel) (both at dilution of 1:20), visualized using biotinylated anti-Myc mAb and ABC-AP VectaStain kit. b , Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst dye; Green, FITC-labeled anti-His tag mAb; Red, Annexin V-PE. c , Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+/Annexin V+) by FACS analysis. d , Expression of E06-scFv in aortic lesion of Ldlr −/− / E06-scFv but not Ldlr −/− mouse. Cross-sections at the AV were stained with biotinylated anti-Myc mAb to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using Hematoxylin QS (Original ×200). Panels a-c are representative of similar studies with 5 other plasma samples. Panel d is representative of studies in 3 other aortic sections.

    Article Snippet: Biotinylated antibodies (E06, anti-myc and anti-polyHis) were revealed with ABC-HRP VectaStain kit (Vector Laboratories, Burlingame, California) and/or NovaRed substrate (Vector Labs).

    Techniques: Mouse Assay, Staining, Microscopy, Binding Assay, Labeling, FACS, Expressing

    Figure 6. Immunohistochemical detection of LC3B is feasible in liver from starved Gfp-Lc3 tg/tg mice using Vectastain ABC. ( A ) Western blot analysis of LC3B in liver from wild-type mice, heterozygous Gfp-Lc3 tg/+ or homozygous Gfp-Lc3 tg/tg transgenic mice. The GFP-LC3 expression in each group was quantified. **p

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 6. Immunohistochemical detection of LC3B is feasible in liver from starved Gfp-Lc3 tg/tg mice using Vectastain ABC. ( A ) Western blot analysis of LC3B in liver from wild-type mice, heterozygous Gfp-Lc3 tg/+ or homozygous Gfp-Lc3 tg/tg transgenic mice. The GFP-LC3 expression in each group was quantified. **p

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Immunohistochemistry, Mouse Assay, Western Blot, Transgenic Assay, Expressing

    Figure 15. SQSTM1 accumulates in cytoplasmic inclusions of liver from autophagy-deficient mice. Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for SQSTM1 using rabbit polyclonal anti-SQSTM1 (Sigma, 1:5,000) and Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The positive area of SQSTM1 inclusions was quantified. ***p

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 15. SQSTM1 accumulates in cytoplasmic inclusions of liver from autophagy-deficient mice. Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for SQSTM1 using rabbit polyclonal anti-SQSTM1 (Sigma, 1:5,000) and Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The positive area of SQSTM1 inclusions was quantified. ***p

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Mouse Assay, Isolation, Staining

    Figure 5. The highest expression of LC3A and LC3B in nonstarved control mice was found in brain tissue. ( A ) Western blot analysis of LC3A and LC3B in different tissue lysates. GAPDH served as a loading control. ( B ) Immunohistochemical detection of LC3A and LC3B in mouse brain using the Vectastain ABC system. Tissue samples were isolated from fed control mice. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for LC3A ( A ) and LC3B ( B ) using rabbit polyclonal anti-LC3A (Abgent, 1:3000) and biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100). Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm.

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 5. The highest expression of LC3A and LC3B in nonstarved control mice was found in brain tissue. ( A ) Western blot analysis of LC3A and LC3B in different tissue lysates. GAPDH served as a loading control. ( B ) Immunohistochemical detection of LC3A and LC3B in mouse brain using the Vectastain ABC system. Tissue samples were isolated from fed control mice. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for LC3A ( A ) and LC3B ( B ) using rabbit polyclonal anti-LC3A (Abgent, 1:3000) and biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100). Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm.

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Expressing, Mouse Assay, Western Blot, Immunohistochemistry, Isolation, Staining

    Figure 13. ATG5 and CTSD are not suitable targets for the immunohistochemical detection of autophagy in liver. Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for ATG5 ( A ) or CTSD ( B ) using rabbit polyclonal anti-ATG5 (Abcam, 1:100) and rabbit monoclonal anti-CTSD (clone EPR3057Y, Abcam, 1:1000), respectively, in combination with Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The ATG5- and CTSD-positive area was quantified. Neither the effect of starvation nor the results between Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice were statistically significant.

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 13. ATG5 and CTSD are not suitable targets for the immunohistochemical detection of autophagy in liver. Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for ATG5 ( A ) or CTSD ( B ) using rabbit polyclonal anti-ATG5 (Abcam, 1:100) and rabbit monoclonal anti-CTSD (clone EPR3057Y, Abcam, 1:1000), respectively, in combination with Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The ATG5- and CTSD-positive area was quantified. Neither the effect of starvation nor the results between Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice were statistically significant.

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Immunohistochemistry, Isolation, Mouse Assay, Staining

    Figure 7. Optimal immunohistochemical detection of LC3B requires processing of tissue samples in a suitable fixative. Liver samples were isolated from Gfp-Lc3 tg/tg mice that underwent starvation for 48 h. After fixation in different fixatives for 24 h, tissues were paraffin-embedded and stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100) and Vectastain ABC. For formalin-fixed samples, heat-mediated antigen retrieval was performed either in citrate buffer (pH 6.0) or in EDTA buffer (pH 8.0). Scale bar, 20 μm.

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 7. Optimal immunohistochemical detection of LC3B requires processing of tissue samples in a suitable fixative. Liver samples were isolated from Gfp-Lc3 tg/tg mice that underwent starvation for 48 h. After fixation in different fixatives for 24 h, tissues were paraffin-embedded and stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100) and Vectastain ABC. For formalin-fixed samples, heat-mediated antigen retrieval was performed either in citrate buffer (pH 6.0) or in EDTA buffer (pH 8.0). Scale bar, 20 μm.

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Immunohistochemistry, Isolation, Mouse Assay, Staining

    Figure 4. Liver from autophagy-deficient Atg7 F/F Alb-Cre + mice but not from autophagy-competent Atg7 +/+ Alb-Cre + shows immunohistochemical staining for LC3A and LC3B. Liver samples were isolated from fed mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for LC3A ( A ) and LC3B ( B ) using rabbit polyclonal anti-LC3A (Abgent, 1:100) and biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100) in combination with Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The LC3A and LC3B positive area was quantified. ***p

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 4. Liver from autophagy-deficient Atg7 F/F Alb-Cre + mice but not from autophagy-competent Atg7 +/+ Alb-Cre + shows immunohistochemical staining for LC3A and LC3B. Liver samples were isolated from fed mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for LC3A ( A ) and LC3B ( B ) using rabbit polyclonal anti-LC3A (Abgent, 1:100) and biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:100) in combination with Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar, 20 μm. The LC3A and LC3B positive area was quantified. ***p

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Mouse Assay, Immunohistochemistry, Staining, Isolation

    Figure 9. LC3B dots are detectable in frozen liver sections using a staining procedure with alkaline phosphatase and are localized on the surface of lipid droplets. Liver samples were isolated from Atg7 +/+ Alb-Cre + ( A ) or Atg7 F/F Alb-Cre + mice ( B ) that were fed normal chow (control) or underwent starvation for 48 h. After fixation in 4% paraformaldehyde, frozen sections were stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:1,000) and Vectastain ABC-AP kit, containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase. LC3B-positive dots (arrows) were detected on the surface of lipid droplets. These structures could be stained using oil red O. Scale bar, 20 μm.

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 9. LC3B dots are detectable in frozen liver sections using a staining procedure with alkaline phosphatase and are localized on the surface of lipid droplets. Liver samples were isolated from Atg7 +/+ Alb-Cre + ( A ) or Atg7 F/F Alb-Cre + mice ( B ) that were fed normal chow (control) or underwent starvation for 48 h. After fixation in 4% paraformaldehyde, frozen sections were stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:1,000) and Vectastain ABC-AP kit, containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase. LC3B-positive dots (arrows) were detected on the surface of lipid droplets. These structures could be stained using oil red O. Scale bar, 20 μm.

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Staining, Isolation, Mouse Assay

    Figure 8. Immunohistochemical staining of LC3B is enhanced in frozen liver sections. Liver samples were isolated from Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice ( A ) or from transgenic Gfp-Lc3 tg/+ or Gfp-Lc3 tg/tg mice ( B ). Some animals were fed normal chow (control), others underwent starvation for 48 h. After fixation in acetone, frozen sections were stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:1,000 [ Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + samples] or 1:30,000 [ Gfp-Lc3 tg/+ and Gfp-Lc3 tg/tg samples]) and Vectastain ABC. Scale bar, 40 μm.

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 8. Immunohistochemical staining of LC3B is enhanced in frozen liver sections. Liver samples were isolated from Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice ( A ) or from transgenic Gfp-Lc3 tg/+ or Gfp-Lc3 tg/tg mice ( B ). Some animals were fed normal chow (control), others underwent starvation for 48 h. After fixation in acetone, frozen sections were stained for LC3B using biotinylated mouse monoclonal anti-LC3B (clone 5F10, Nanotools, 1:1,000 [ Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + samples] or 1:30,000 [ Gfp-Lc3 tg/+ and Gfp-Lc3 tg/tg samples]) and Vectastain ABC. Scale bar, 40 μm.

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Immunohistochemistry, Staining, Isolation, Mouse Assay, Transgenic Assay

    Figure 14. BECN1 is upregulated in mouse tissue after starvation. ( A ) Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for BECN1 using rabbit polyclonal anti-BECN1 (Lifespan, 1:1,000) and Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar 20 μm. The BECN1 positive area was quantified. ***p

    Journal: Autophagy

    Article Title: Immunohistochemical analysis of macroautophagy

    doi: 10.4161/auto.22968

    Figure Lengend Snippet: Figure 14. BECN1 is upregulated in mouse tissue after starvation. ( A ) Liver samples were isolated from fed Atg7 +/+ Alb-Cre + and Atg7 F/F Alb-Cre + mice (control) or from mice that underwent starvation for 48 h. After fixation in neutral buffered formalin for 24 h, tissues were paraffin-embedded and stained for BECN1 using rabbit polyclonal anti-BECN1 (Lifespan, 1:1,000) and Vectastain ABC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). Scale bar 20 μm. The BECN1 positive area was quantified. ***p

    Article Snippet: In some experiments, frozen sections were stained using a Vectastain ABC-AP kit (Vector Laboratories, AK-5000) containing biotinylated alkaline phosphatase instead of biotinylated horseradish peroxidase.

    Techniques: Isolation, Mouse Assay, Staining

    Immunohistochemical determination of satellite cells per fiber (A), myonuclei per fiber (B), and relative satellite cell number [C; (satellite cells / satellite cells + myonuclei)]. (D) Transverse sections of mounted muscle were cut 6 μm thick from the midbelly of the gastrocnemius and soleus muscles and stained with antibodies against Pax7. Pax7-stained satellite cells were identified as any nuclei localized to the myofiber membrane and stained brown or with a brown rim (arrows). Sections were counterstained with hematoxylin to identify myonuclei, which are stained blue (arrow heads). Images were captured by light microscopy at 40× magnification and analyzed by a single investigator blinded to the sample. Values are mean ± SE. * Significantly greater than control within muscle (p

    Journal: Molecules and Cells

    Article Title: ?-Hydroxy-?-Methylbutyrate Did Not Enhance High Intensity Resistance Training-Induced Improvements in Myofiber Dimensions and Myogenic Capacity in Aged Female Rats

    doi: 10.1007/s10059-012-0196-x

    Figure Lengend Snippet: Immunohistochemical determination of satellite cells per fiber (A), myonuclei per fiber (B), and relative satellite cell number [C; (satellite cells / satellite cells + myonuclei)]. (D) Transverse sections of mounted muscle were cut 6 μm thick from the midbelly of the gastrocnemius and soleus muscles and stained with antibodies against Pax7. Pax7-stained satellite cells were identified as any nuclei localized to the myofiber membrane and stained brown or with a brown rim (arrows). Sections were counterstained with hematoxylin to identify myonuclei, which are stained blue (arrow heads). Images were captured by light microscopy at 40× magnification and analyzed by a single investigator blinded to the sample. Values are mean ± SE. * Significantly greater than control within muscle (p

    Article Snippet: Sections were incubated at 37°C with Pax7 antibody ( ) (MO15020, 1:200 in 1% goat serum; Neuromics) for 1 h, and then incubated in biotinylated goat anti-mouse secondary Ab (BA-9200, 1:200 in 1% goat serum; Vector Labs) at room temperature for 30 min. Pax7 cells were presented by DAB substrate (Elite Pk-6102; Vector Labs) after the application of Vectastain ABC reagent at room temperature for 1 h. Sections were rinsed with PBS followed by 5 min in deionized (DI) water.

    Techniques: Immunohistochemistry, Staining, Light Microscopy